##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_50330665-1.341000000356c2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2794520 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66504337059674 33.0 33.0 33.0 33.0 33.0 2 32.623441950674895 33.0 33.0 33.0 33.0 33.0 3 32.70519445199891 33.0 33.0 33.0 33.0 33.0 4 36.636686085624724 37.0 37.0 37.0 37.0 37.0 5 36.65954582540114 37.0 37.0 37.0 37.0 37.0 6 36.55411984884703 37.0 37.0 37.0 37.0 37.0 7 36.369086283154175 37.0 37.0 37.0 37.0 37.0 8 36.32757790246625 37.0 37.0 37.0 37.0 37.0 9 36.41596803744471 37.0 37.0 37.0 37.0 37.0 10-11 36.23668214934944 37.0 37.0 37.0 35.0 37.0 12-13 36.285778595250704 37.0 37.0 37.0 35.0 37.0 14-15 38.12877721397592 40.0 37.0 40.0 35.0 40.0 16-17 38.11571307415943 40.0 37.0 40.0 37.0 40.0 18-19 37.99241282939467 40.0 37.0 40.0 37.0 40.0 20-21 38.07335982565879 40.0 37.0 40.0 35.0 40.0 22-23 37.77005585932468 40.0 37.0 40.0 33.0 40.0 24-25 37.97456289452214 40.0 37.0 40.0 35.0 40.0 26-27 37.92203580579134 40.0 37.0 40.0 33.0 40.0 28-29 37.654030030201966 40.0 37.0 40.0 33.0 40.0 30-31 37.52103187667292 40.0 37.0 40.0 33.0 40.0 32-33 37.50687076850407 40.0 37.0 40.0 33.0 40.0 34-35 37.3375241544165 40.0 37.0 40.0 33.0 40.0 36-37 37.31501975294505 40.0 37.0 40.0 33.0 40.0 38-39 37.27382466398522 40.0 37.0 40.0 33.0 40.0 40-41 37.159069893935275 40.0 37.0 40.0 33.0 40.0 42-43 36.55027643387773 37.0 37.0 40.0 33.0 40.0 44-45 36.370729141319444 37.0 37.0 40.0 33.0 40.0 46-47 36.784619362180266 37.0 37.0 40.0 33.0 40.0 48-49 36.49985632595222 37.0 37.0 40.0 33.0 40.0 50-51 36.494714476904804 37.0 37.0 40.0 33.0 40.0 52-53 36.45472174112191 37.0 37.0 40.0 33.0 40.0 54-55 35.903420086454915 37.0 35.0 40.0 30.0 40.0 56-57 35.846330317907906 37.0 37.0 40.0 33.0 40.0 58-59 35.79631994045489 37.0 37.0 40.0 33.0 40.0 60-61 35.48722821808396 37.0 33.0 40.0 27.0 40.0 62-63 35.099423872436056 37.0 33.0 37.0 27.0 40.0 64-65 34.84129761103875 37.0 33.0 37.0 27.0 40.0 66-67 33.8692083076879 37.0 33.0 37.0 27.0 40.0 68-69 33.6955292858881 37.0 33.0 37.0 27.0 37.0 70-71 33.23371115611984 37.0 33.0 37.0 27.0 37.0 72-73 31.864620042082358 33.0 30.0 37.0 18.5 37.0 74-75 30.674384867526445 33.0 27.0 37.0 15.0 37.0 76-77 28.116023682063467 30.0 24.5 33.0 15.0 35.0 78-79 30.023713732590927 33.0 27.0 37.0 15.0 37.0 80-81 30.323378433505574 33.0 27.0 37.0 15.0 37.0 82-83 30.08166393513018 33.0 27.0 37.0 6.0 37.0 84-85 29.937076492564017 33.0 27.0 37.0 6.0 37.0 86-87 29.78589847272519 33.0 27.0 37.0 6.0 37.0 88-89 29.639343787126233 33.0 27.0 37.0 6.0 37.0 90-91 29.54262198874941 33.0 27.0 37.0 6.0 37.0 92-93 29.311153078167273 33.0 27.0 37.0 6.0 37.0 94-95 29.1377748235833 33.0 27.0 37.0 6.0 37.0 96-97 28.96335059330404 33.0 27.0 37.0 6.0 37.0 98-99 28.835395166253953 33.0 27.0 37.0 6.0 37.0 100-101 27.331177805132903 33.0 21.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15302641304590736 2306 2 0.18596557332293884 2306 3 0.16692822481068248 2306 4 0.1339495672346871 2306 5 0.16662418514938793 2306 6 0.16087493129406738 2306 7 0.2207237160397142 2306 8 0.08747930742139687 2306 9 0.21367794404712726 2306 10-11 0.24040549336473305 2306 12-13 0.2871091927863674 2306 14-15 0.32740122680114325 2306 16-17 0.3789938748371213 2306 18-19 0.5360788349070233 2306 20-21 0.47831618748574556 2306 22-23 0.4607003321890346 2306 24-25 0.38573398274596116 2306 26-27 0.48828915743767 2306 28-29 0.5430825504139776 2306 30-31 0.6667971505845642 2306 32-33 0.5193478534910341 2306 34-35 0.5473087611097824 2306 36-37 0.524591272758208 2306 38-39 0.6094420267666507 2306 40-41 0.6965073036224325 2306 42-43 0.7146391704792165 2306 44-45 0.7805949054396208 2306 46-47 0.638784555220127 2306 48-49 0.6273154922467086 2306 50-51 0.6541658386249694 2306 52-53 0.6466457153320917 2306 54-55 0.7928826113925496 2306 56-57 0.8078455803471414 2306 58-59 0.6905590840425688 2306 60-61 0.7165077044201098 2306 62-63 0.6242429585679403 2306 64-65 0.5770100951573482 2306 66-67 0.5503015468040502 2306 68-69 0.5624436714131775 2306 70-71 0.5995917040616376 2306 72-73 0.06338591749359068 2306 74-75 0.2587512827376841 2306 76-77 0.043699327574412195 2306 78-79 0.5143045153069252 2306 80-81 0.6696360952718123 2306 82-83 0.625934323356919 2306 84-85 0.5802722680775325 2306 86-87 0.6498202295003672 2306 88-89 0.48872499283215376 2306 90-91 0.41413315821529295 2306 92-93 0.3847226988884245 2306 94-95 0.25966995540546023 2306 96-97 0.4195346403224143 2306 98-99 0.5854288310797138 2306 100-101 0.4005762628878422 2307 1 0.12792212658078483 2307 2 0.22021988459462705 2307 3 0.15020304494730397 2307 4 0.16745968153846036 2307 5 0.13221901461108843 2307 6 0.1322215595999765 2307 7 0.1021281337545048 2307 8 0.16831967559375016 2307 9 0.1450794792347878 2307 10-11 0.1654048726745856 2307 12-13 0.22203319716781067 2307 14-15 0.33648266008621164 2307 16-17 0.4595383105708635 2307 18-19 0.49974258159474516 2307 20-21 0.41531950386477234 2307 22-23 0.40562859934417617 2307 24-25 0.39781724191208667 2307 26-27 0.29609361016358804 2307 28-29 0.333214797392273 2307 30-31 0.5862282028500445 2307 32-33 0.5499040086963944 2307 34-35 0.4867059426294489 2307 36-37 0.38432855460666104 2307 38-39 0.42585198019595083 2307 40-41 0.5566909563692306 2307 42-43 0.5187267412326335 2307 44-45 0.3927807573231732 2307 46-47 0.43112993078396045 2307 48-49 0.5371265836219692 2307 50-51 0.504927835723926 2307 52-53 0.41601240518522076 2307 54-55 0.5116611605093411 2307 56-57 0.5601752724385349 2307 58-59 0.6265726626389849 2307 60-61 0.43555104626636165 2307 62-63 0.3497580998307086 2307 64-65 0.2587389128962059 2307 66-67 0.3452118455053963 2307 68-69 0.30929877119280036 2307 70-71 0.08869722433253457 2307 72-73 0.07211871227827871 2307 74-75 0.2267072596050923 2307 76-77 0.16049438242027847 2307 78-79 0.6643302686880013 2307 80-81 0.7921465491667377 2307 82-83 0.7209373678946953 2307 84-85 0.6398714621481965 2307 86-87 0.5346378118217636 2307 88-89 0.6972466898453078 2307 90-91 0.730955712790788 2307 92-93 0.6576522553958455 2307 94-95 0.6351282666990308 2307 96-97 0.8335322341486737 2307 98-99 0.702187065323546 2307 100-101 0.3014185920202621 2201 1 0.15044722198212668 2201 2 0.15991971905413038 2201 3 0.09165016464795173 2201 4 0.14026976175713202 2201 5 0.12313613550949043 2201 6 0.12058105441859368 2201 7 0.07890350001725466 2201 8 0.10278448194882372 2201 9 0.17523063491659485 2201 10-11 0.2504226771542051 2201 12-13 0.2027153270259987 2201 14-15 0.3414545322466509 2201 16-17 0.3608746316702707 2201 18-19 0.36310407593035876 2201 20-21 0.4877009461622208 2201 22-23 0.43348840608744155 2201 24-25 0.30770817947682616 2201 26-27 0.34256384818218777 2201 28-29 0.32559849085323833 2201 30-31 0.4913030355051049 2201 32-33 0.5412606331839811 2201 34-35 0.6494261479730241 2201 36-37 0.543118002150841 2201 38-39 0.6854086960565908 2201 40-41 0.7386818237460488 2201 42-43 0.6197524836971908 2201 44-45 0.732145219137081 2201 46-47 0.6000950666204119 2201 48-49 0.5301530486436974 2201 50-51 0.647428827679434 2201 52-53 0.6843721653056036 2201 54-55 0.8599884328677447 2201 56-57 0.6586060319935925 2201 58-59 0.5865535492072596 2201 60-61 0.624121104855142 2201 62-63 0.633460787844804 2201 64-65 0.4872349366859794 2201 66-67 0.6241058414533072 2201 68-69 0.6163056834742804 2201 70-71 0.4952618434547844 2201 72-73 0.10337818559965228 2201 74-75 0.0059234216326835565 2201 76-77 8.609436294904071E-4 2201 78-79 0.4240066629560779 2201 80-81 0.5565997407665044 2201 82-83 0.5734483125679652 2201 84-85 0.4120877540956265 2201 86-87 0.45399865694834673 2201 88-89 0.357105927750375 2201 90-91 0.4326312885248811 2201 92-93 0.15206550737516977 2201 94-95 0.478544822855806 2201 96-97 0.3842109189853886 2201 98-99 0.27150095593884416 2201 100-101 0.3328102285381149 1101 1 0.12855971488666995 1101 2 0.17895355538460223 1101 3 0.147489978739749 1101 4 0.15785569243630704 1101 5 0.1051363631404314 1101 6 0.12800391202247852 1101 7 0.22105469882550466 1101 8 0.1279874048453209 1101 9 0.15618095086257 1101 10-11 0.1490134543553836 1101 12-13 0.16704732386798327 1101 14-15 0.2561107591053755 1101 16-17 0.29563729760314317 1101 18-19 0.29869910752495343 1101 20-21 0.3442594184993055 1101 22-23 0.2618797108737496 1101 24-25 0.24483107569152196 1101 26-27 0.29562163042295 1101 28-29 0.3147762965486294 1101 30-31 0.4360184320007292 1101 32-33 0.36193193554990444 1101 34-35 0.41957562802361537 1101 36-37 0.36900896837213537 1101 38-39 0.43761243006840544 1101 40-41 0.4917649263269226 1101 42-43 0.39573356583737507 1101 44-45 0.44917547100062905 1101 46-47 0.38900987214002924 1101 48-49 0.39324384457400186 1101 50-51 0.31286897659040136 1101 52-53 0.2641539277453475 1101 54-55 0.40868812715074654 1101 56-57 0.44533241096164744 1101 58-59 0.3457389257923964 1101 60-61 0.4444464586195451 1101 62-63 0.2676567896082176 1101 64-65 0.3026572208904028 1101 66-67 0.5339375170187068 1101 68-69 0.5701747611079568 1101 70-71 0.22872510410950042 1101 72-73 -0.09524457518904583 1101 74-75 0.25115091570419423 1101 76-77 0.1240885198210222 1101 78-79 0.5841059489440994 1101 80-81 0.5397968317007447 1101 82-83 0.6565395479124199 1101 84-85 0.7123542119565265 1101 86-87 0.675598816001866 1101 88-89 0.6818889940255595 1101 90-91 0.6306666030157544 1101 92-93 0.6029782642163468 1101 94-95 0.6252837575430199 1101 96-97 0.7482819326346011 1101 98-99 0.5261873799144361 1101 100-101 0.5539477421167298 2304 1 0.1477427073631219 2304 2 0.24586414651302846 2304 3 0.12698113664399102 2304 4 0.14470541531581205 2304 5 0.08664252034091646 2304 6 0.09353348172219711 2304 7 0.12023834194422989 2304 8 0.13875951805177067 2304 9 0.109789492600612 2304 10-11 0.09410887303705096 2304 12-13 0.14152837026010445 2304 14-15 0.1528934990263977 2304 16-17 0.2178442164534431 2304 18-19 0.3381746699321724 2304 20-21 0.25475079284169055 2304 22-23 0.3134675104015656 2304 24-25 0.20037843182654314 2304 26-27 0.14416090531532433 2304 28-29 0.3735024053625722 2304 30-31 0.3981478978632609 2304 32-33 0.19563357888639388 2304 34-35 0.273546106126183 2304 36-37 0.40401402080859583 2304 38-39 0.372620071795545 2304 40-41 0.493956528526887 2304 42-43 0.43139822348003776 2304 44-45 0.3032151628352082 2304 46-47 0.325579553272199 2304 48-49 0.3167482304340794 2304 50-51 0.2751715597730353 2304 52-53 0.18181514760666317 2304 54-55 0.566543570370925 2304 56-57 0.4930039083152664 2304 58-59 0.36432820719119263 2304 60-61 0.3266818663066928 2304 62-63 0.2678835185240942 2304 64-65 0.13727656860160664 2304 66-67 0.15904822579387456 2304 68-69 0.11231546714778062 2304 70-71 0.10814482414742344 2304 72-73 -0.09532789723572677 2304 74-75 0.14512394326221312 2304 76-77 0.1363112997366258 2304 78-79 0.35799962470116853 2304 80-81 0.5479265405582439 2304 82-83 0.5071343212631767 2304 84-85 0.5589669549846974 2304 86-87 0.6415708888419331 2304 88-89 0.48288457629728043 2304 90-91 0.5202174208332622 2304 92-93 0.502555280344307 2304 94-95 0.38849561330757254 2304 96-97 0.5032054024965866 2304 98-99 0.6000218339458918 2304 100-101 0.444441825255538 2202 1 0.09061841851752916 2202 2 0.1613559031663243 2202 3 0.08511404415150992 2202 4 0.06190647275825256 2202 5 0.0638302894691094 2202 6 0.11064757473852183 2202 7 0.1305663046619827 2202 8 0.007876182516213248 2202 9 0.0976899108357756 2202 10-11 0.1960416292939442 2202 12-13 0.1347495026156551 2202 14-15 0.10524486529563148 2202 16-17 0.14178571975725163 2202 18-19 0.23751100391304902 2202 20-21 0.24570222864497993 2202 22-23 0.132866856106709 2202 24-25 0.12586922324967986 2202 26-27 0.08526616881847104 2202 28-29 0.10870486473153562 2202 30-31 0.17794071074074225 2202 32-33 0.22586917938606632 2202 34-35 0.2435750068040008 2202 36-37 0.19873791344126346 2202 38-39 0.1841734082588502 2202 40-41 0.17528696242126784 2202 42-43 0.047726028762191675 2202 44-45 0.2305516055216188 2202 46-47 0.2173104827138772 2202 48-49 0.26834592062893137 2202 50-51 0.2079653013331253 2202 52-53 0.08072017364445827 2202 54-55 0.2869571574379606 2202 56-57 0.33056387206759297 2202 58-59 0.17261639492939906 2202 60-61 0.1806915073628943 2202 62-63 0.12300528277740597 2202 64-65 0.20105860276325416 2202 66-67 0.13293968706799575 2202 68-69 0.09474944962182263 2202 70-71 0.16938938067102072 2202 72-73 0.010965965680973966 2202 74-75 -0.01822251907553607 2202 76-77 0.007773098644509702 2202 78-79 0.41643564259259946 2202 80-81 0.24603979672939147 2202 82-83 0.4411458459238702 2202 84-85 0.35785023991595466 2202 86-87 0.40434168041500484 2202 88-89 0.3410175306606362 2202 90-91 0.2639221053857348 2202 92-93 0.2589733580743854 2202 94-95 0.3372763308203197 2202 96-97 0.28917264277236043 2202 98-99 0.28070753157770767 2202 100-101 0.3521337256625259 1102 1 0.06878812486662866 1102 2 0.11033198818876855 1102 3 0.056324221517158435 1102 4 0.07430572608180341 1102 5 -0.0015669570042007308 1102 6 0.08594284887467296 1102 7 -0.06234296104033632 1102 8 -0.0030642748794420527 1102 9 0.05970825470811292 1102 10-11 0.09665382322441474 1102 12-13 0.048261089663022005 1102 14-15 0.08882883911075368 1102 16-17 0.10979220804145484 1102 18-19 0.22210593749768037 1102 20-21 0.12177121618852027 1102 22-23 0.004906122896066734 1102 24-25 -0.0072320428786767366 1102 26-27 0.04368755879598041 1102 28-29 0.11306208670772122 1102 30-31 0.12313937603976655 1102 32-33 0.2121627741000509 1102 34-35 0.19765212269818022 1102 36-37 0.20097577749833562 1102 38-39 0.11681393422713171 1102 40-41 0.33598070027896654 1102 42-43 0.021701495650766844 1102 44-45 0.05244909654184937 1102 46-47 0.04280397665375091 1102 48-49 0.1105399286367259 1102 50-51 0.14030678218289694 1102 52-53 0.12260777744888429 1102 54-55 0.13083373314897528 1102 56-57 0.2475205495885362 1102 58-59 0.10907654928876553 1102 60-61 0.16514767172301248 1102 62-63 0.01841630083437451 1102 64-65 0.029184981220069517 1102 66-67 0.03168160542487897 1102 68-69 0.1360644200633203 1102 70-71 0.026356641814217596 1102 72-73 -0.16305656716388484 1102 74-75 -0.07250666958184127 1102 76-77 -0.35607307804755806 1102 78-79 0.06318845005421281 1102 80-81 0.07463142150560031 1102 82-83 0.06282604643040557 1102 84-85 0.02982912709650165 1102 86-87 0.17781624673008878 1102 88-89 0.14023163014054063 1102 90-91 0.10827307140827003 1102 92-93 0.07885814669966607 1102 94-95 -0.02146881500002351 1102 96-97 0.1813963577817752 1102 98-99 0.3016798261593827 1102 100-101 0.01708315060783505 2305 1 0.07000489761092155 2305 2 0.12180031618194676 2305 3 0.06370675614838461 2305 4 0.0817551940974397 2305 5 0.04076759237631222 2305 6 0.029010971823140608 2305 7 0.12797953271254414 2305 8 0.07098989653967891 2305 9 -0.03744446441982063 2305 10-11 0.08158197595006556 2305 12-13 0.0880917590839303 2305 14-15 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3266.0 19 4247.0 20 5982.0 21 8241.0 22 11495.0 23 15727.0 24 21428.0 25 29022.0 26 40546.0 27 62407.0 28 113202.0 29 177447.0 30 123288.0 31 66091.0 32 80376.0 33 115315.0 34 179914.0 35 316362.0 36 574329.0 37 725703.0 38 114940.0 39 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.98315243803661 19.798250732206625 19.874446096259906 12.344150733496859 2 29.174527289123 31.083692369351446 28.65644189341998 11.085338448105578 3 37.21985225783988 14.178860124467562 34.74091444402735 13.860373173665208 4 29.41169102505649 13.713287949593559 28.38826645900182 28.486754566348132 5 43.80033064712366 12.26096073744328 29.32331849476833 14.615390120664728 6 38.32969526072456 13.199368764582111 33.646923264102604 14.82401271059073 7 32.126841103302176 28.26549818931337 23.185663369737917 16.421997337646538 8 27.810357413795572 37.60377453015187 18.752558578933055 15.833309477119506 9 42.61898286646723 21.377123799436042 19.0136767673876 16.990216566709133 10-11 31.165048022558434 30.731288378683995 23.024222406710276 15.079441192047295 12-13 24.15634885418605 29.103388059487855 22.549042411576945 24.191220674749154 14-15 23.270722700141704 29.457187638664244 22.830557662854446 24.44153199833961 16-17 29.473397935960378 23.09042340008302 23.8009210884159 23.635257575540702 18-19 21.092495312253984 23.295342312812217 31.116613944434107 24.495548430499692 20-21 27.37928649272263 24.143918122596748 31.18448448829933 17.29231089638129 22-23 20.275668389206437 37.78058963319958 24.186326295384855 17.75741568220913 24-25 20.716813620943846 22.218860484090293 32.21886048409029 24.84546541087557 26-27 19.27540328929476 22.32165094542175 25.476843966047834 32.926101799235646 28-29 25.938676409544392 30.637390321056923 23.827043642557577 19.5968896268411 30-31 17.4000722843279 24.66047836479968 31.14794311724375 26.791506233628677 32-33 17.8673081602565 30.616724875828403 24.14309076335113 27.37287620056396 34-35 18.988448821264477 30.268149807480356 31.460715972689407 19.282685398565764 36-37 17.76651317446867 32.22212520611946 32.05486785452546 17.956493764886407 38-39 17.74155137168597 25.20382721185201 30.056656691582013 26.997964724880006 40-41 26.21148153019075 24.166324459200368 22.661828345156266 26.960365665452613 42-43 19.592432598592882 31.313144506748863 30.293402938377312 18.801019956280943 44-45 18.062781604169075 25.297201877557946 29.989629559120278 26.6503869591527 46-47 25.984167014822958 24.47369056703701 23.835468642226243 25.706673775913785 48-49 18.77947049242483 30.819525938506526 31.509470726819977 18.89153284224867 50-51 25.173506770024918 23.980014224432924 29.707128766902695 21.139350238639466 52-53 17.500705053047188 24.631869256680826 23.33117164784855 34.53625404242344 54-55 25.601321218110694 24.610139182124037 31.160194653454965 18.628344946310303 56-57 18.993412068746434 24.432938582440418 29.97655476788325 26.597094580929898 58-59 18.049738397917206 24.12773654962995 30.212284596150667 27.610240456302183 60-61 24.37863658898468 23.79336389067369 31.698652840096575 20.129346680245057 62-63 17.764188873639892 23.500127936500554 31.674590379533026 27.06109281032653 64-65 26.00782370204869 25.152892516878257 30.173879729146236 18.66540405192682 66-67 19.488505348039578 38.31144983738609 23.08335367647743 19.116691138096897 68-69 18.618001417434442 36.81644581892633 24.809362226087952 19.756190537551273 70-71 18.11080217885239 39.054506210015674 23.762305710493983 19.072385900637958 72-73 19.93710815643138 38.580638117754866 23.243708578454623 18.238545147359133 74-75 21.254867356426967 36.284118479353275 23.477451532271996 18.983562631947756 76-77 20.641100848213185 34.428654208263374 23.98416816661185 20.946076776911593 78-79 20.433887983548917 34.37056842446569 24.537729354863703 20.65781423712169 80-81 19.657083896052907 34.70573382539495 24.619621848867315 21.017560429684824 82-83 19.3633537427455 33.69956114216839 24.87606014961176 22.061024965474346 84-85 20.138003129325906 32.72167609329571 24.77639491921157 22.363925858166812 86-87 19.002516823268607 33.112173141404234 25.52812660075651 22.35718343457065 88-89 18.633608618236806 33.31651798895878 25.67749613020409 22.372377262600324 90-91 19.462357239980715 32.34614810570174 24.89508532225315 23.296409332064393 92-93 20.024105111604733 32.04440381378452 24.16604566080245 23.765445413808294 94-95 20.36636299026672 33.173582480455565 23.663379275652122 22.79667525362559 96-97 19.885066461948984 33.94885267826538 22.935462559521135 23.230618300264496 98-99 19.834736925756726 32.194082359839435 24.603872640722578 23.367308073681265 100-101 20.830996848631766 33.052370488645536 23.826810237946468 22.28982242477623 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1215.0 1 718.0 2 363.0 3 571.0 4 489.0 5 312.5 6 278.0 7 300.0 8 350.5 9 367.0 10 334.0 11 312.5 12 291.0 13 264.5 14 278.5 15 288.5 16 289.0 17 316.5 18 312.0 19 315.5 20 337.0 21 379.5 22 438.0 23 461.5 24 537.0 25 657.5 26 824.5 27 1089.5 28 1416.0 29 1904.0 30 2479.0 31 3430.0 32 5802.5 33 10294.5 34 18041.5 35 31873.0 36 52096.0 37 79863.5 38 116500.5 39 147578.5 40 165170.5 41 167878.0 42 183652.5 43 214189.0 44 205501.0 45 176681.5 46 170614.5 47 165169.0 48 142860.5 49 118193.0 50 96815.5 51 84152.0 52 79695.5 53 80300.5 54 71860.0 55 46676.0 56 28079.0 57 22868.5 58 21983.5 59 19333.5 60 14991.0 61 11181.5 62 7505.0 63 4739.0 64 3214.0 65 2198.0 66 1465.0 67 1097.5 68 854.5 69 654.5 70 476.5 71 343.0 72 239.0 73 164.5 74 113.0 75 67.5 76 55.0 77 42.5 78 23.0 79 15.0 80 16.0 81 10.5 82 6.0 83 4.0 84 2.5 85 2.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15473140288851037 2 0.0 3 0.0020397062822953495 4 0.009804903883314486 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.7892160371011837E-5 22-23 1.0735296222607102E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.588236978085682E-4 38-39 0.002057598442666361 40-41 0.005814952120578847 42-43 0.0030953437441850478 44-45 0.0013955885089389235 46-47 0.0013419120278258879 48-49 0.003345833989379214 50-51 0.0011629904241157694 52-53 0.014456865579777566 54-55 0.02628358358501639 56-57 0.027732848575068345 58-59 0.02082647467185778 60-61 0.03095343744185048 62-63 0.0059044129224339065 64-65 0.01139730615633454 66-67 0.028126476103230606 68-69 0.02648039734909752 70-71 0.030327211828865063 72-73 0.029879907819589772 74-75 0.03712623276984956 76-77 0.018929905672530525 78-79 0.01061005110001002 80-81 0.015226228475731074 82-83 0.007908334883987234 84-85 0.011021570788543291 86-87 0.032885790761919755 88-89 0.015924022730200535 90-91 0.020862258992599803 92-93 0.04782574467171464 94-95 0.0545710891315861 96-97 0.03862917424101456 98-99 0.03485392840273106 100-101 0.034764467600876 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2794520.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.499606760753764 #Duplication Level Percentage of deduplicated Percentage of total 1 75.53819518896418 18.506560775466845 2 9.73000559395861 4.767626216638406 3 4.188838641542339 3.0787469850611195 4 2.2399230645499095 2.19508937023261 5 1.477568835542225 1.809992771636468 6 0.8973775497326211 1.3191238250626782 7 0.8164347445897644 1.4001631131785965 8 0.5913783672130423 1.1590829954828936 9 0.4594399487469601 1.0130488267033249 >10 3.3081349295895275 15.828815857622208 >50 0.377287297237965 6.488290859655292 >100 0.33245714299823426 16.53749498372563 >500 0.03039727614756762 4.964000702405623 >1k 0.011234851411974585 5.0398864757243045 >5k 5.89585677818671E-4 1.03719574221906 >10k+ 7.369820972324231E-4 14.854880499184908 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 351541 12.579655897971746 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 19722 0.7057383736741909 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 18335 0.6561055208050041 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11118 0.3978500780098192 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 10645 0.380924094298842 TruSeq Adapter, Index 27 (97% over 39bp) TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 8356 0.29901378412034985 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7579 0.27120936690379743 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7517 0.26899073901779197 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5270 0.18858337031046477 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 4883 0.1747348381833016 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4741 0.16965346463793424 No Hit GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA 4000 0.1431372829680947 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 3729 0.13343973204700627 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 3641 0.1302907118217082 No Hit GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA 3554 0.12717747591715214 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG 3464 0.12395688705037 TruSeq Adapter, Index 27 (97% over 38bp) GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 3372 0.12066472954210383 No Hit CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC 2963 0.10602894235861614 TruSeq Adapter, Index 27 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.006262256129854143 0.0 0.0 2 0.006799020940984498 0.0 0.0 3 0.007335785752114853 0.0 0.0 4 0.0075504916765669955 0.0 0.0 5 0.008946080185505918 0.0 0.0 6 0.009590197958862345 0.0 0.0 7 0.010878433505575198 0.0 0.0 8 0.012452943618224238 0.0 0.0 9 0.014134806693099352 0.0 0.0 10-11 0.022740935831556047 0.0 0.0 12-13 0.030237751027010006 0.0 0.0 14-15 0.03692941900576843 0.0 0.0 16-17 0.04367476346563989 0.0 0.0 18-19 0.050259078482172245 0.0 0.0 20-21 0.05589510899904098 0.0 0.0 22-23 0.06094069822366632 0.0 0.0 24-25 0.06663040522164809 0.0 0.0 26-27 0.07121079827662712 0.0 0.0 28-29 0.07811717217983769 0.0 0.0 30-31 0.15830983496271273 0.0 0.0 32-33 0.16983238624164437 0.0 0.0 34-35 0.17802699569156777 0.0 0.0 36-37 0.18885175271602994 0.0 0.0 38-39 0.20128680417388317 0.0 0.0 40-41 0.21583313055551578 0.0 0.0 42-43 0.23283068290797704 0.0 0.0 44-45 0.2510627943260381 0.0 0.0 46-47 0.26872235661222676 0.0 0.0 48-49 0.28877946838813107 0.0 0.0 50-51 0.31236133575712466 0.0 0.0 52-53 0.3359074188053762 0.0 0.0 54-55 0.36372972818229965 0.0 0.0 56-57 0.3936990968037445 0.0 0.0 58-59 0.4252071912170963 0.0 0.0 60-61 0.46097361979874896 0.0 0.0 62-63 0.4983503428137927 0.0 0.0 64-65 0.5402716745630735 0.0 0.0 66-67 0.5858251148676696 0.0 0.0 68-69 0.6369609092080215 0.0 0.0 70-71 0.6898322431043613 0.0 0.0 72-73 0.7474271073386485 0.0 0.0 74-75 0.8105148648068362 0.0 0.0 76-77 0.8805447804989766 0.0 0.0 78-79 0.9634391594978744 0.0 0.0 80-81 1.0560847658989738 0.0 0.0 82-83 1.1618990023331377 0.0 0.0 84-85 1.2855875069779426 0.0 0.0 86-87 1.4231961123913948 0.0 0.0 88-89 1.567675307387315 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGCGA 15 6.5752416E-4 95.158394 1 TCGGAAG 45585 0.0 82.67606 3 GATCGGA 45795 0.0 82.37918 1 CGGAAGA 45490 0.0 82.34462 4 GAAGAGC 45565 0.0 81.95158 6 ATCGGAA 46570 0.0 80.92739 2 GGAAGAG 47495 0.0 78.941376 5 AGTACGC 40 8.961979E-8 71.3688 1 GAGCACA 53375 0.0 69.75552 9 AGAGCAC 53385 0.0 69.72466 8 AAGAGCA 53570 0.0 69.43955 7 GCGATCG 45 2.0252628E-7 63.438927 1 GTCGCGC 25 0.0049890196 57.095036 1 ATTGCGG 25 0.0049890196 57.095036 1 AATGTCG 25 0.005026292 56.987774 9 CGTGGCA 170 0.0 55.97552 1 CGATTGC 35 2.7657856E-4 54.376225 1 CGAGGTG 275 0.0 53.634727 1 CACGCCG 90 3.6379788E-12 52.865772 1 ATCGGAC 130 0.0 51.149273 2 >>END_MODULE