FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_50330665-1.341000000356c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_50330665-1.341000000356c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2794520
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT35154112.579655897971746TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC197220.7057383736741909No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA183350.6561055208050041No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC111180.3978500780098192No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT106450.380924094298842TruSeq Adapter, Index 27 (97% over 39bp)
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT83560.29901378412034985No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT75790.27120936690379743No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC75170.26899073901779197No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT52700.18858337031046477No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC48830.1747348381833016No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT47410.16965346463793424No Hit
GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA40000.1431372829680947No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT37290.13343973204700627No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC36410.1302907118217082No Hit
GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA35540.12717747591715214No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG34640.12395688705037TruSeq Adapter, Index 27 (97% over 38bp)
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC33720.12066472954210383No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC29630.10602894235861614TruSeq Adapter, Index 27 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA156.5752416E-495.1583941
TCGGAAG455850.082.676063
GATCGGA457950.082.379181
CGGAAGA454900.082.344624
GAAGAGC455650.081.951586
ATCGGAA465700.080.927392
GGAAGAG474950.078.9413765
AGTACGC408.961979E-871.36881
GAGCACA533750.069.755529
AGAGCAC533850.069.724668
AAGAGCA535700.069.439557
GCGATCG452.0252628E-763.4389271
GTCGCGC250.004989019657.0950361
ATTGCGG250.004989019657.0950361
AATGTCG250.00502629256.9877749
CGTGGCA1700.055.975521
CGATTGC352.7657856E-454.3762251
CGAGGTG2750.053.6347271
CACGCCG903.6379788E-1252.8657721
ATCGGAC1300.051.1492732
Produced by FastQC (version 0.11.2)