##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_50220371-2.34100000035905.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3312039 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67179613525082 33.0 33.0 33.0 33.0 33.0 2 32.6659350931556 33.0 33.0 33.0 33.0 33.0 3 32.72869341212468 33.0 33.0 33.0 33.0 33.0 4 36.6842697202539 37.0 37.0 37.0 37.0 37.0 5 36.67712366913554 37.0 37.0 37.0 37.0 37.0 6 36.55358073984032 37.0 37.0 37.0 37.0 37.0 7 36.5082600174696 37.0 37.0 37.0 37.0 37.0 8 36.44518980603791 37.0 37.0 37.0 37.0 37.0 9 36.43552687634415 37.0 37.0 37.0 37.0 37.0 10-11 36.29827064234449 37.0 37.0 37.0 37.0 37.0 12-13 36.36444468196178 37.0 37.0 37.0 37.0 37.0 14-15 38.298134925343575 40.0 37.0 40.0 37.0 40.0 16-17 38.12507763344574 40.0 37.0 40.0 37.0 40.0 18-19 37.85799457675468 40.0 37.0 40.0 35.0 40.0 20-21 37.79419505627802 40.0 37.0 40.0 33.0 40.0 22-23 37.67805783687933 40.0 37.0 40.0 33.0 40.0 24-25 37.62045963226882 40.0 37.0 40.0 33.0 40.0 26-27 37.506007930462175 40.0 37.0 40.0 33.0 40.0 28-29 37.35023183603816 40.0 37.0 40.0 33.0 40.0 30-31 37.21436734289662 40.0 37.0 40.0 33.0 40.0 32-33 37.1064327744933 40.0 37.0 40.0 33.0 40.0 34-35 37.003157269585294 37.0 37.0 40.0 33.0 40.0 36-37 36.92320531249783 37.0 37.0 40.0 33.0 40.0 38-39 36.793719065506174 37.0 37.0 40.0 33.0 40.0 40-41 36.59749568166317 37.0 37.0 40.0 33.0 40.0 42-43 36.5533197223825 37.0 37.0 40.0 33.0 40.0 44-45 36.415181101430264 37.0 37.0 40.0 33.0 40.0 46-47 36.280016630238954 37.0 37.0 40.0 33.0 40.0 48-49 36.11384965575587 37.0 37.0 40.0 33.0 40.0 50-51 35.93531779064196 37.0 37.0 40.0 33.0 40.0 52-53 35.72152441441662 37.0 37.0 40.0 27.0 40.0 54-55 35.37808265542767 37.0 37.0 40.0 27.0 40.0 56-57 35.2134349867257 37.0 33.0 40.0 27.0 40.0 58-59 35.04551501356113 37.0 33.0 40.0 27.0 40.0 60-61 34.74987009512871 37.0 33.0 40.0 27.0 40.0 62-63 34.45632418579612 37.0 33.0 37.0 27.0 40.0 64-65 34.221571968204486 37.0 33.0 37.0 27.0 40.0 66-67 33.83142801156629 37.0 33.0 37.0 24.5 40.0 68-69 33.57795122581588 37.0 33.0 37.0 24.5 40.0 70-71 33.316369161111936 37.0 33.0 37.0 22.0 40.0 72-73 33.029075140721474 37.0 33.0 37.0 22.0 37.0 74-75 32.69385097820406 37.0 33.0 37.0 22.0 37.0 76-77 29.870675737815887 33.0 30.0 35.0 18.5 37.0 78-79 32.34090012225097 35.0 33.0 37.0 22.0 37.0 80-81 32.6797518084781 37.0 33.0 37.0 22.0 37.0 82-83 32.530578142346755 37.0 33.0 37.0 22.0 37.0 84-85 32.37610909774915 37.0 33.0 37.0 22.0 37.0 86-87 32.21326062283687 37.0 33.0 37.0 22.0 37.0 88-89 31.98483894664284 35.0 33.0 37.0 22.0 37.0 90-91 31.8381466824515 33.0 33.0 37.0 18.5 37.0 92-93 31.638191307529894 33.0 33.0 37.0 15.0 37.0 94-95 31.479510506971685 33.0 33.0 37.0 15.0 37.0 96-97 31.27503571063022 33.0 33.0 37.0 15.0 37.0 98-99 31.0961436444438 33.0 33.0 37.0 15.0 37.0 100-101 29.532469122495236 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.05542903413534361 2306 2 0.13676010692235963 2306 3 0.10449527144039195 2306 4 0.11557716767815407 2306 5 0.048915047465349915 2306 6 0.11598594080003721 2306 7 0.1017655624904279 2306 8 0.11117438769121435 2306 9 0.1412318767010774 2306 10-11 0.11854702691845631 2306 12-13 0.12647126735583925 2306 14-15 0.2285190030080102 2306 16-17 0.40350180398839086 2306 18-19 0.5660814055217145 2306 20-21 0.525450272732499 2306 22-23 0.5630412713357416 2306 24-25 0.47263355014943187 2306 26-27 0.49229684685965225 2306 28-29 0.5200332531514817 2306 30-31 0.7320825035589067 2306 32-33 0.6526956544537299 2306 34-35 0.7203400186004671 2306 36-37 0.6585329989531132 2306 38-39 0.6348410970149843 2306 40-41 0.793493384430981 2306 42-43 0.6791640799883609 2306 44-45 0.7273561619256697 2306 46-47 0.6794374026262275 2306 48-49 0.6066452677726275 2306 50-51 0.6488174546955321 2306 52-53 0.7028390493973404 2306 54-55 0.6709212767237531 2306 56-57 0.7956494289837934 2306 58-59 0.6893759589527306 2306 60-61 0.8533756901317275 2306 62-63 0.5821092937390304 2306 64-65 0.67474029594608 2306 66-67 0.8547458754718704 2306 68-69 0.7937695273587408 2306 70-71 0.7699318714387005 2306 72-73 0.4603778626038775 2306 74-75 0.704434975322485 2306 76-77 0.41553675284862024 2306 78-79 0.6306881000523603 2306 80-81 0.8129694035177337 2306 82-83 0.9895789447813215 2306 84-85 0.9787390255975126 2306 86-87 1.1609756213294773 2306 88-89 1.056345525368819 2306 90-91 1.0134508494091818 2306 92-93 0.9779875162564551 2306 94-95 1.0054789836812965 2306 96-97 1.1857529828719642 2306 98-99 1.1337613947951155 2306 100-101 0.8290230784039139 2307 1 0.10013608568092991 2307 2 0.20698907254676158 2307 3 0.12021824666626912 2307 4 0.15576673684208941 2307 5 0.12346726477066028 2307 6 0.1748200386469918 2307 7 0.18737436243604577 2307 8 0.1749354709805786 2307 9 0.1799412460663632 2307 10-11 0.20676742049460017 2307 12-13 0.19028778732437246 2307 14-15 0.2516717071849399 2307 16-17 0.35722431629775997 2307 18-19 0.5378924611331968 2307 20-21 0.5018488147073725 2307 22-23 0.45737734224289994 2307 24-25 0.5046880901302018 2307 26-27 0.5251210259921493 2307 28-29 0.47140962045467916 2307 30-31 0.591036215145742 2307 32-33 0.5973605417513639 2307 34-35 0.5531733427617951 2307 36-37 0.5437576267585342 2307 38-39 0.6918251474372781 2307 40-41 0.7403372974026823 2307 42-43 0.6488928206288733 2307 44-45 0.724111295281233 2307 46-47 0.651935953988918 2307 48-49 0.6395289300572387 2307 50-51 0.7380717221058859 2307 52-53 0.6650223319609267 2307 54-55 0.7444327411908702 2307 56-57 0.8589552840682657 2307 58-59 0.7632119505490138 2307 60-61 0.5517366258057592 2307 62-63 0.7689341397302698 2307 64-65 0.6896653067096992 2307 66-67 0.7937129242334962 2307 68-69 0.6985786795093603 2307 70-71 0.6230254809249303 2307 72-73 0.6788534105772328 2307 74-75 0.7235444437454248 2307 76-77 0.6172692097698693 2307 78-79 0.8120931001980267 2307 80-81 0.9185111291280208 2307 82-83 0.9038661504813419 2307 84-85 0.9887860132411959 2307 86-87 1.1543758239768422 2307 88-89 1.013872936176469 2307 90-91 1.0543667604644682 2307 92-93 1.0511101146747706 2307 94-95 1.1528984669628848 2307 96-97 1.2083654829124235 2307 98-99 1.1134804724582636 2307 100-101 0.8962082870886157 2201 1 0.17299238975870423 2201 2 0.17522681154465403 2201 3 0.13278149858497557 2201 4 0.14524475576089912 2201 5 0.12698941505389172 2201 6 0.0727203424875853 2201 7 0.2118359890027932 2201 8 0.1721591835627052 2201 9 0.15111471287167433 2201 10-11 0.19900328814678403 2201 12-13 0.16670760218551806 2201 14-15 0.28123108770298444 2201 16-17 0.3333633931487512 2201 18-19 0.5007636390831323 2201 20-21 0.5434323867794859 2201 22-23 0.5497575016399026 2201 24-25 0.5084333123416584 2201 26-27 0.42843904919163833 2201 28-29 0.4684039341089914 2201 30-31 0.6095920395284722 2201 32-33 0.600547180285183 2201 34-35 0.5757844123356506 2201 36-37 0.6370049466415111 2201 38-39 0.4704215586184972 2201 40-41 0.7701745235882171 2201 42-43 0.572857289645377 2201 44-45 0.6733680355242697 2201 46-47 0.6572455720406012 2201 48-49 0.6231000621635445 2201 50-51 0.7398855825011807 2201 52-53 0.7158436849698973 2201 54-55 0.7554115316420891 2201 56-57 0.7893920928887539 2201 58-59 0.7310064380331269 2201 60-61 0.7846499307266441 2201 62-63 0.7489433518416675 2201 64-65 0.6622468020797783 2201 66-67 0.7543295210741903 2201 68-69 0.7673887769668468 2201 70-71 0.6227194349164336 2201 72-73 0.6453060318350907 2201 74-75 0.6716992014321903 2201 76-77 0.45030240229432295 2201 78-79 0.7744772799513413 2201 80-81 0.8140937626349469 2201 82-83 0.9408273974281158 2201 84-85 0.9298792970769085 2201 86-87 0.9987506151858483 2201 88-89 0.9709136936231673 2201 90-91 0.9838458423192122 2201 92-93 0.8834585450727133 2201 94-95 0.9089546709656027 2201 96-97 1.0513148915006632 2201 98-99 0.8970390590282449 2201 100-101 1.1039486299179906 1101 1 0.019438482646727095 1101 2 0.10376354415677724 1101 3 0.10809763660087413 1101 4 0.06629294382225481 1101 5 0.05832842464617016 1101 6 0.08139317578275751 1101 7 0.10517598396031502 1101 8 0.11539659413386971 1101 9 0.03813426057514846 1101 10-11 0.10712896616364986 1101 12-13 0.021849659008246647 1101 14-15 0.08960530444170445 1101 16-17 0.25650548235437753 1101 18-19 0.37798620440984365 1101 20-21 0.40735830396717176 1101 22-23 0.46901133033900777 1101 24-25 0.29728411277744016 1101 26-27 0.44421125402732287 1101 28-29 0.3390562412746334 1101 30-31 0.40593485411096 1101 32-33 0.2267699237178178 1101 34-35 0.37804173144827047 1101 36-37 0.4326035328968203 1101 38-39 0.4735291600405702 1101 40-41 0.5600771898570329 1101 42-43 0.4928420465539247 1101 44-45 0.47750529736542546 1101 46-47 0.4544725553194695 1101 48-49 0.46004697965886265 1101 50-51 0.4792813408045049 1101 52-53 0.2801196843870031 1101 54-55 0.6285428651003215 1101 56-57 0.6512761528711337 1101 58-59 0.6619979097663347 1101 60-61 0.4804311333811171 1101 62-63 0.37073195167465656 1101 64-65 0.2939370504334633 1101 66-67 0.5418248660329752 1101 68-69 0.5025886372577872 1101 70-71 0.5232033278955939 1101 72-73 0.39271540020671125 1101 74-75 0.5058519376711956 1101 76-77 0.2538423153693792 1101 78-79 0.6511470229930723 1101 80-81 0.7179287070173217 1101 82-83 0.7680577115190701 1101 84-85 0.7871285551616438 1101 86-87 0.8399813271238017 1101 88-89 0.7509817902259712 1101 90-91 0.669793053573656 1101 92-93 0.6667180916659134 1101 94-95 0.7856128486875527 1101 96-97 0.8420045777798002 1101 98-99 0.894556563139723 1101 100-101 0.8392641434066235 2304 1 0.0835847160266141 2304 2 0.1706526295942794 2304 3 0.10398282880181142 2304 4 0.0827374944110204 2304 5 0.023237686384142364 2304 6 0.04734309584154772 2304 7 0.09424740678057475 2304 8 0.07997970423115675 2304 9 0.027256384462525318 2304 10-11 0.04951583013768612 2304 12-13 0.1083274422546836 2304 14-15 0.04944302167685777 2304 16-17 0.14620561354149686 2304 18-19 0.4155389713562556 2304 20-21 0.39662163411011875 2304 22-23 0.4589014618833076 2304 24-25 0.3419037004006853 2304 26-27 0.2838557351177968 2304 28-29 0.40043207753954846 2304 30-31 0.4466820153052353 2304 32-33 0.5437540547378461 2304 34-35 0.4418851205436951 2304 36-37 0.44367377037153943 2304 38-39 0.4887012651912528 2304 40-41 0.6603110806902208 2304 42-43 0.5853447813846131 2304 44-45 0.561264973701924 2304 46-47 0.5760889779853571 2304 48-49 0.622312869373296 2304 50-51 0.5659607932208743 2304 52-53 0.5120321165308184 2304 54-55 0.7046204973635142 2304 56-57 0.7708242746917193 2304 58-59 0.7580467680388381 2304 60-61 0.7483172590870737 2304 62-63 0.5498974151368969 2304 64-65 0.47082762512021503 2304 66-67 0.6915094751262387 2304 68-69 0.5142069002967347 2304 70-71 0.5660223465771921 2304 72-73 0.45016326588853417 2304 74-75 0.4565469660607704 2304 76-77 0.4493974663253759 2304 78-79 0.6816775407977715 2304 80-81 0.8474736414812725 2304 82-83 0.8505577311941082 2304 84-85 0.9763900388776747 2304 86-87 0.915213235318248 2304 88-89 0.9708577890369909 2304 90-91 0.8838395647651183 2304 92-93 0.8681342859802612 2304 94-95 0.8739829168358462 2304 96-97 0.8688421660677612 2304 98-99 0.943221868898938 2304 100-101 0.924259109623069 2202 1 0.09601584726091517 2202 2 0.15331502303978795 2202 3 0.08326056609428178 2202 4 0.07213146013329919 2202 5 0.037765425577191536 2202 6 0.07383035165813112 2202 7 0.10845367694705743 2202 8 0.12418603956027852 2202 9 0.044589103818978515 2202 10-11 0.1630317292789485 2202 12-13 0.1076623760548685 2202 14-15 0.028395896055236847 2202 16-17 0.23232143687688733 2202 18-19 0.38304249828417625 2202 20-21 0.40740958075929257 2202 22-23 0.3908851049744939 2202 24-25 0.3294241044524995 2202 26-27 0.2599853023401195 2202 28-29 0.44745588253817914 2202 30-31 0.4725971974402512 2202 32-33 0.46909097274043887 2202 34-35 0.4452203616943393 2202 36-37 0.36476534234421365 2202 38-39 0.529673225707036 2202 40-41 0.6086941240679025 2202 42-43 0.5783450919931994 2202 44-45 0.6067208026630269 2202 46-47 0.5168211128329219 2202 48-49 0.37827457220342353 2202 50-51 0.4568633283734016 2202 52-53 0.4853580611447512 2202 54-55 0.6874800077966015 2202 56-57 0.6657090617130592 2202 58-59 0.6725805347761309 2202 60-61 0.5018683514844469 2202 62-63 0.5562488720106344 2202 64-65 0.5061867744436626 2202 66-67 0.5273160474301548 2202 68-69 0.5076404067092852 2202 70-71 0.5208164434935867 2202 72-73 0.43253283471820225 2202 74-75 0.4122682730495697 2202 76-77 0.29810977109538683 2202 78-79 0.6598776509226383 2202 80-81 0.7571056843409139 2202 82-83 0.7591209709937132 2202 84-85 0.7050318974030674 2202 86-87 0.8855628763263965 2202 88-89 0.7075282278965105 2202 90-91 0.6098318831758505 2202 92-93 0.6794845047638205 2202 94-95 0.8185179705352859 2202 96-97 0.9426398067457562 2202 98-99 0.9472872031344153 2202 100-101 0.8602881308910675 1102 1 0.1321506547921345 1102 2 0.15696000675863786 1102 3 0.09287261914909806 1102 4 0.07916917738612739 1102 5 0.09407521547606024 1102 6 0.03987835744742796 1102 7 0.03549763943638595 1102 8 0.0771613210551294 1102 9 0.09329112879036217 1102 10-11 0.125496775958446 1102 12-13 0.07530006125116273 1102 14-15 0.11040752648720087 1102 16-17 0.21395491849055048 1102 18-19 0.3997454864612564 1102 20-21 0.3376536608060121 1102 22-23 0.2551817396173348 1102 24-25 0.32250345393135404 1102 26-27 0.36259017236648106 1102 28-29 0.4578345622770712 1102 30-31 0.4409732648485445 1102 32-33 0.5148757023068526 1102 34-35 0.3450634507563919 1102 36-37 0.45865349020887436 1102 38-39 0.42004215009463053 1102 40-41 0.642139667845079 1102 42-43 0.4195056667864492 1102 44-45 0.4925277101160148 1102 46-47 0.4440408326829868 1102 48-49 0.5209773454960498 1102 50-51 0.47436307486511 1102 52-53 0.3603654525155022 1102 54-55 0.6149633007824846 1102 56-57 0.653493233183795 1102 58-59 0.5605026323704081 1102 60-61 0.43038335401701033 1102 62-63 0.46416553962087903 1102 64-65 0.5444509258048456 1102 66-67 0.5784691767541119 1102 68-69 0.6161011295312449 1102 70-71 0.5528444167180808 1102 72-73 0.39416897848344945 1102 74-75 0.5610321590762624 1102 76-77 0.31428668239063384 1102 78-79 0.5685596242060029 1102 80-81 0.8285538193125035 1102 82-83 0.8187509646352495 1102 84-85 0.8611733150214249 1102 86-87 0.9637681709885868 1102 88-89 0.9540581837697175 1102 90-91 0.9241614676059342 1102 92-93 0.8320001876799239 1102 94-95 0.7985871235710142 1102 96-97 1.0067769010098253 1102 98-99 1.1570100198115583 1102 100-101 1.0944008984240874 2305 1 0.04440814519828962 2305 2 0.08522708857149297 2305 3 0.01417609644973794 2305 4 -0.02372286595811346 2305 5 -0.05289166488066144 2305 6 -0.012928660033914241 2305 7 0.03585147340380246 2305 8 0.01781354193636986 2305 9 -0.09497787957064219 2305 10-11 0.026837615465460374 2305 12-13 0.08867110124153044 2305 14-15 0.0012589526851414234 2305 16-17 0.09341862782266475 2305 18-19 0.2846814477392954 2305 20-21 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-1.5514002092956893 1213 76-77 -1.2539054400210397 1213 78-79 -1.6060910830242179 1213 80-81 -1.7200963193304553 1213 82-83 -1.732933274345278 1213 84-85 -1.8975898660532593 1213 86-87 -1.9645676395679956 1213 88-89 -2.00479416017518 1213 90-91 -1.9343113133786503 1213 92-93 -2.1900811261845945 1213 94-95 -2.1866632053408424 1213 96-97 -2.4621412890679046 1213 98-99 -2.560929496948127 1213 100-101 -2.346345031171026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 1.0 8 25.0 9 95.0 10 135.0 11 108.0 12 167.0 13 434.0 14 1333.0 15 3520.0 16 6698.0 17 10389.0 18 12788.0 19 14056.0 20 16167.0 21 18607.0 22 21354.0 23 24271.0 24 28788.0 25 33038.0 26 38065.0 27 44953.0 28 52723.0 29 62464.0 30 74561.0 31 89727.0 32 112044.0 33 148721.0 34 215882.0 35 361143.0 36 704978.0 37 958879.0 38 255875.0 39 49.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.799653070531434 31.478133645263405 20.142805991670308 15.579407292534848 2 30.37431020588828 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19.37577699527859 29.932964225193707 33.598049538992235 17.09320924053547 22-23 19.24922000383148 29.566953615080514 33.717814920763985 17.466011460324022 24-25 19.24387061867327 29.010014676759543 34.06152524170156 17.684589462865624 26-27 19.295077745159404 29.44307117156531 33.80754272519134 17.45430835808395 28-29 19.09344968462026 29.838779072347883 33.67978154846607 17.387989694565796 30-31 18.443291277669132 30.95888967491023 33.43352840953865 17.164290637881983 32-33 18.22686870535039 31.345690675743853 33.48835868176673 16.939081937139026 34-35 18.57298479879011 30.757805086232377 33.9656175546242 16.703592560353307 36-37 18.67032224813127 30.39814457250017 34.324291601512435 16.607241577856126 38-39 19.037794700490085 30.165123005520407 33.658118370385374 17.138963923604134 40-41 18.867297562887444 30.081424291774127 33.14703762957827 17.90424051576015 42-43 19.05563760667967 29.738799644138158 33.13573127932271 18.06983146985946 44-45 18.697548702430947 29.550277913563434 33.42233290388499 18.329840480120627 46-47 18.85689346108289 29.898919112645196 33.236085979442635 18.008101446829272 48-49 18.712059520563685 30.55302178698771 32.7771623261475 17.95775636630111 50-51 19.276787040626665 30.4321210188852 31.850397885031818 18.440694055456323 52-53 19.23247363958995 30.371706883262284 31.832640178043647 18.56317929910412 54-55 19.59013104734741 30.470158010939763 31.769910074547024 18.1698008671658 56-57 19.534658403739307 30.486762005735567 31.721761490045058 18.256818100480068 58-59 19.286033427942808 31.12270698358047 31.57975877679417 18.011500811682552 60-61 19.2035861952323 31.01306844093832 31.3966801871321 18.38666517669728 62-63 18.719878037899377 30.878601481921653 31.813985452535043 18.587535027643927 64-65 18.67173338462658 30.471510355629487 32.15549100953988 18.70126525020405 66-67 18.95278832818699 30.45467230106841 31.69316248918784 18.899376881556755 68-69 19.282164373202285 30.528777330400214 31.685037938503502 18.504020357894 70-71 18.876685713647152 30.739301608701965 31.896043625181157 18.487969052469726 72-73 18.882984988797926 30.593607236980798 31.686753283153035 18.83665449106824 74-75 19.176625325815753 30.236819479458976 31.27640940657019 19.310145788155083 76-77 19.208062517883445 29.959827835738807 30.93086023997602 19.901249406401725 78-79 18.926868258144427 30.13757967630336 31.15434427410128 19.781207791450928 80-81 18.657552257519402 30.559396509109227 30.720862512920753 20.062188720450617 82-83 18.230967519466006 30.78302759316009 30.52093439245558 20.465070494918322 84-85 18.16095265185486 30.91278087690787 31.034561893321882 19.89170457791539 86-87 18.412846887994 30.602134825453962 31.32544203197738 19.65957625457466 88-89 18.800591206817753 30.50429989870246 31.171559141140587 19.523549753339204 90-91 18.665534992923515 30.531393905058092 30.73989085015807 20.063180251860324 92-93 18.27500088735299 30.649353591665076 30.663490824014865 20.412154696967065 94-95 18.046823170871708 30.51995132555686 30.68816092051251 20.745064583058927 96-97 18.076246945500788 31.014290072462238 30.376779462891424 20.532683519145557 98-99 18.655055224122226 30.77775280953175 30.055526157459422 20.5116658088866 100-101 18.756581551434678 30.791852097843325 29.612009189744853 20.83955716097714 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 148424.0 1 83083.0 2 14540.5 3 11458.5 4 10157.0 5 8334.5 6 7983.0 7 8244.0 8 8352.0 9 7907.0 10 7083.0 11 6295.5 12 5718.5 13 5325.0 14 5092.5 15 4869.0 16 4788.0 17 4835.5 18 4845.5 19 4905.0 20 5110.0 21 5368.5 22 5864.5 23 6524.0 24 7367.5 25 8662.0 26 10475.5 27 12904.5 28 15795.0 29 19256.5 30 23763.0 31 29346.0 32 37680.5 33 52601.0 34 73116.0 35 94883.0 36 123162.0 37 161350.5 38 190344.5 39 194168.5 40 193652.0 41 193143.5 42 185038.0 43 179369.5 44 169314.5 45 156555.0 46 152218.0 47 141842.5 48 118232.0 49 95296.0 50 76464.0 51 61664.0 52 50162.5 53 39224.5 54 30753.5 55 25590.5 56 20352.5 57 14905.5 58 10930.0 59 7843.5 60 5606.0 61 4347.5 62 3343.5 63 2540.5 64 2050.5 65 1535.0 66 1044.0 67 744.0 68 562.5 69 455.0 70 338.5 71 239.0 72 197.5 73 173.5 74 137.5 75 94.0 76 69.0 77 53.0 78 39.5 79 32.5 80 21.0 81 14.5 82 18.5 83 17.5 84 9.0 85 5.5 86 7.0 87 6.0 88 4.0 89 3.0 90 1.5 91 1.0 92 0.5 93 1.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16047516348690338 2 0.0 3 0.0015700298215087442 4 0.010537315532818304 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.5289321774290704E-5 22-23 1.0567508414001164E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.850149107543721E-4 38-39 0.0016153191432830352 40-41 0.005721550984152058 42-43 0.002400334054037407 44-45 0.0011473294849486976 46-47 0.0011473294849486976 48-49 0.0032608311677489303 50-51 8.152077919372326E-4 52-53 0.014839801101375921 54-55 0.027400039673445876 56-57 0.028517176277211714 58-59 0.02136146343687378 60-61 0.03046461711350621 62-63 0.005751743865334919 64-65 0.01092982298819549 66-67 0.028713430004900304 68-69 0.026901857133928675 70-71 0.030660870841194808 72-73 0.03155156083608919 74-75 0.039567770790138644 76-77 0.0201235553083765 78-79 0.010809051463464047 80-81 0.015277597878527395 82-83 0.007246291483886512 84-85 0.010778858582281187 86-87 0.03429911302372949 88-89 0.015322887200301687 90-91 0.021723778011068107 92-93 0.049259685649836855 94-95 0.05685319526732626 96-97 0.04073019671567877 98-99 0.035235092340398164 100-101 0.03546153894926962 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3312039.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.095356078033866 #Duplication Level Percentage of deduplicated Percentage of total 1 80.89946837961386 34.05491927963487 2 8.919994294893844 7.509806721151739 3 3.3137835455501605 4.184846949463907 4 1.7081468222686211 2.8762019486783856 5 0.9839726592521969 2.0710339731135554 6 0.6713945804336274 1.695755635932942 7 0.5322023116146362 1.5682272069079592 8 0.4341378825987546 1.462015100396659 9 0.29481899961271296 1.1169459690540193 >10 1.929204924445117 14.923464825930957 >50 0.1616836840549542 4.839754405129185 >100 0.13843745995345105 11.881265335673893 >500 0.010422375325347436 2.8621912686784614 >1k 0.0021963143519870745 1.2242438675518743 >5k 0.0 0.0 >10k+ 1.357660306888431E-4 7.729327512701503 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 168535 5.0885572301533895 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101952 3.0782246223549903 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT 3917 0.11826551559326445 TruSeq Adapter, Index 19 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.5096440591430235E-4 0.0 0.0 2 2.4154304946288373E-4 0.0 0.0 3 2.4154304946288373E-4 0.0 0.0 4 3.019288118286047E-4 0.0 0.0 5 3.019288118286047E-4 0.0 0.0 6 3.6231457419432563E-4 0.0 0.0 7 5.736647424743489E-4 0.0 0.0 8 6.340505048400699E-4 0.0 0.0 9 9.05786435485814E-4 0.0 0.0 10-11 0.002249369648123105 0.0 0.0 12-13 0.007895438429318013 0.0 0.0 14-15 0.017270328036596186 0.0 0.0 16-17 0.03727311182024125 0.0 0.0 18-19 0.05801562119286639 0.0 0.0 20-21 0.06656020656761591 0.0 0.0 22-23 0.07336870127435094 0.0 0.0 24-25 0.08272849444103768 0.0 0.0 26-27 0.09319032777089883 0.0 0.0 28-29 0.10418053652146003 0.0 0.0 30-31 0.11595576018277562 0.0 0.0 32-33 0.13105220077420587 0.0 0.0 34-35 0.14761299610300482 0.0 0.0 36-37 0.16491351702078388 0.0 0.0 38-39 0.18378406776007167 0.0 0.0 40-41 0.203726465781351 0.0 0.0 42-43 0.22496715769349335 0.0 0.0 44-45 0.24711363604112146 0.0 0.0 46-47 0.27105659081912986 0.0 0.0 48-49 0.29647899677509837 0.0 0.0 50-51 0.3249659801711272 0.0 0.0 52-53 0.3542681713590933 0.0 0.0 54-55 0.38595560016050534 0.0 0.0 56-57 0.4178845720113803 0.0 0.0 58-59 0.45330082163887564 0.0 0.0 60-61 0.48897371075642526 0.0 0.0 62-63 0.5268506802003238 0.0 0.0 64-65 0.5675053947130453 0.0 0.0 66-67 0.6134740563139505 0.0 0.0 68-69 0.6645906041565331 0.0 0.0 70-71 0.7196624194340706 0.0 0.0 72-73 0.7803953999333946 0.0 0.0 74-75 0.8483897683571963 0.0 0.0 76-77 0.9251400723240275 0.0 0.0 78-79 1.0118842199623859 0.0 0.0 80-81 1.1105394592273823 0.0 0.0 82-83 1.2294390253254868 0.0 0.0 84-85 1.3567020195112436 0.0 0.0 86-87 1.5027751786739225 0.0 0.0 88-89 1.6739839114213328 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGG 70 1.2769306E-9 54.372395 1 CGGTGCG 90 3.6379788E-12 52.86205 1 CGCGTTG 45 1.5182795E-5 52.767883 6 CGCGGAG 120 0.0 47.57584 1 GGCCCGC 85 7.1268005E-9 44.777264 1 CGCTCGG 45 9.630866E-4 42.219402 3 CGACGGG 160 0.0 41.628864 1 AGCCGAC 70 4.2656357E-6 40.779297 1 CGGAGCG 50 0.0016036365 38.060677 1 GGCACGC 75 6.850805E-6 38.060673 1 GTCGGCT 75 6.850805E-6 38.060673 1 CGGGGTG 485 0.0 36.29498 1 CGGGCGA 105 4.597132E-8 36.24826 1 GTTACGG 95 8.5985084E-7 35.055885 1 GGCGCGT 55 0.0025602137 34.600613 1 GGTCCGG 55 0.0025811687 34.543148 3 CGAGCGG 85 1.6153908E-5 33.582947 1 TCGCCGG 100 1.3008521E-6 33.24778 2 GTTTGAT 13215 0.0 32.023968 3 CCGGGCG 120 1.4863144E-7 31.717228 1 >>END_MODULE