FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_50220371-2.34100000035905.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_50220371-2.34100000035905.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312039
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1685355.0885572301533895No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1019523.0782246223549903No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT39170.11826551559326445TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG701.2769306E-954.3723951
CGGTGCG903.6379788E-1252.862051
CGCGTTG451.5182795E-552.7678836
CGCGGAG1200.047.575841
GGCCCGC857.1268005E-944.7772641
CGCTCGG459.630866E-442.2194023
CGACGGG1600.041.6288641
AGCCGAC704.2656357E-640.7792971
CGGAGCG500.001603636538.0606771
GGCACGC756.850805E-638.0606731
GTCGGCT756.850805E-638.0606731
CGGGGTG4850.036.294981
CGGGCGA1054.597132E-836.248261
GTTACGG958.5985084E-735.0558851
GGCGCGT550.002560213734.6006131
GGTCCGG550.002581168734.5431483
CGAGCGG851.6153908E-533.5829471
TCGCCGG1001.3008521E-633.247782
GTTTGAT132150.032.0239683
CCGGGCG1201.4863144E-731.7172281
Produced by FastQC (version 0.11.2)