FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_50110001-2.341000000358f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_50110001-2.341000000358f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5903582
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT277860.4706634040147152TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC193630.3279873134649438No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147580.24998382338044933No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76600.12975173377789959No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG75380.12768519180389126No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA62460.10580017352177033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGG2550.059.7009931
CGGACGG851.8189894E-1255.969681
CGCGGAG3950.055.40291
CGATTCG601.4773632E-647.5742261
CCGAGTG1300.043.9146771
TGTACCG1750.043.4256942
CAACGGG1750.040.7779121
CACGGGG5900.040.3171421
CGAGTGG2850.040.062511
CAAGGCG1900.040.0625081
GGACGGG7550.039.69771
CGGAGCC1950.039.0352671
CGCGGGG5000.039.0108681
CGAACGG756.853312E-638.0593831
GTTACGG3300.037.4827271
ACGCGGA905.633974E-737.0021741
CGGACCG2600.036.5955621
CGAGGGG8850.036.0166471
ATGACCG1601.8189894E-1235.680671
TGCGGCC3650.035.134662
Produced by FastQC (version 0.11.2)