##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_50110001-2.341000000358f7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5903582 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.684888428753936 33.0 33.0 33.0 33.0 33.0 2 32.66277100919408 33.0 33.0 33.0 33.0 33.0 3 32.72603141618089 33.0 33.0 33.0 33.0 33.0 4 36.67323668918294 37.0 37.0 37.0 37.0 37.0 5 36.66871333370147 37.0 37.0 37.0 37.0 37.0 6 36.54831744523918 37.0 37.0 37.0 37.0 37.0 7 36.49371076746287 37.0 37.0 37.0 37.0 37.0 8 36.42371817652401 37.0 37.0 37.0 37.0 37.0 9 36.40992858234204 37.0 37.0 37.0 37.0 37.0 10-11 36.279148235766016 37.0 37.0 37.0 37.0 37.0 12-13 36.351553514459525 37.0 37.0 37.0 37.0 37.0 14-15 38.25392067053528 40.0 37.0 40.0 37.0 40.0 16-17 38.21554092074947 40.0 37.0 40.0 37.0 40.0 18-19 38.12800465886643 40.0 37.0 40.0 37.0 40.0 20-21 38.099737413658346 40.0 37.0 40.0 37.0 40.0 22-23 38.029590001460136 40.0 37.0 40.0 37.0 40.0 24-25 38.0124122947729 40.0 37.0 40.0 35.0 40.0 26-27 37.94064383284589 40.0 37.0 40.0 33.0 40.0 28-29 37.82732966189002 40.0 37.0 40.0 33.0 40.0 30-31 37.718941313934494 40.0 37.0 40.0 33.0 40.0 32-33 37.62444902095034 40.0 37.0 40.0 33.0 40.0 34-35 37.529387836062924 40.0 37.0 40.0 33.0 40.0 36-37 37.45669688334981 40.0 37.0 40.0 33.0 40.0 38-39 37.37303106825652 40.0 37.0 40.0 33.0 40.0 40-41 37.188851785238185 40.0 37.0 40.0 33.0 40.0 42-43 37.148564888232265 40.0 37.0 40.0 33.0 40.0 44-45 37.028021885695836 40.0 37.0 40.0 33.0 40.0 46-47 36.92124264556671 38.5 37.0 40.0 33.0 40.0 48-49 36.76707014487137 37.0 37.0 40.0 33.0 40.0 50-51 36.604704567498175 37.0 37.0 40.0 33.0 40.0 52-53 36.42723333054407 37.0 37.0 40.0 33.0 40.0 54-55 36.100975136789835 37.0 37.0 40.0 30.0 40.0 56-57 35.98070205512518 37.0 37.0 40.0 30.0 40.0 58-59 35.81364906932774 37.0 37.0 40.0 27.0 40.0 60-61 35.51716127598465 37.0 35.0 40.0 27.0 40.0 62-63 35.21930143428176 37.0 33.0 38.5 27.0 40.0 64-65 34.97133511485061 37.0 33.0 37.0 27.0 40.0 66-67 34.57269713540017 37.0 33.0 37.0 27.0 40.0 68-69 34.36247044590894 37.0 33.0 37.0 27.0 40.0 70-71 34.13618409975503 37.0 33.0 37.0 27.0 40.0 72-73 33.84876834098349 37.0 33.0 37.0 27.0 37.0 74-75 33.5316988059114 37.0 33.0 37.0 27.0 37.0 76-77 30.760173653893517 33.0 30.0 35.0 24.5 37.0 78-79 33.25267066672403 35.0 33.0 37.0 27.0 37.0 80-81 33.64481572374196 37.0 33.0 37.0 27.0 37.0 82-83 33.551611547023484 37.0 33.0 37.0 27.0 37.0 84-85 33.47127125531584 37.0 33.0 37.0 27.0 37.0 86-87 33.365711020868346 37.0 33.0 37.0 27.0 37.0 88-89 33.20852153827964 37.0 33.0 37.0 27.0 37.0 90-91 33.120927091382825 37.0 33.0 37.0 27.0 37.0 92-93 32.971138962751766 37.0 33.0 37.0 27.0 37.0 94-95 32.8637657611938 37.0 33.0 37.0 27.0 37.0 96-97 32.712411888240055 33.0 33.0 37.0 27.0 37.0 98-99 32.58084634040824 33.0 33.0 37.0 27.0 37.0 100-101 30.92983835915212 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15599276578942778 2306 2 0.20281342633565913 2306 3 0.16410650435831542 2306 4 0.16807683114976868 2306 5 0.16299976287510987 2306 6 0.17798283267418213 2306 7 0.19984726491106386 2306 8 0.17686792688392927 2306 9 0.19912842415954657 2306 10-11 0.22080357450193588 2306 12-13 0.21309863217383196 2306 14-15 0.38124908149335823 2306 16-17 0.4330760255228796 2306 18-19 0.5153231345859268 2306 20-21 0.49441509399773764 2306 22-23 0.47023661294388575 2306 24-25 0.4249454949461793 2306 26-27 0.4641899926604296 2306 28-29 0.4555029569934348 2306 30-31 0.4893480643304784 2306 32-33 0.5225830608217521 2306 34-35 0.44964042830114437 2306 36-37 0.47482647029271163 2306 38-39 0.5242978413030954 2306 40-41 0.5505201089105043 2306 42-43 0.5520483654838912 2306 44-45 0.5886896402756463 2306 46-47 0.4838451834341271 2306 48-49 0.48045984257293384 2306 50-51 0.5721816039607219 2306 52-53 0.5257836840734456 2306 54-55 0.6500527346089697 2306 56-57 0.6540181986824294 2306 58-59 0.3835132328066919 2306 60-61 0.5452025843153194 2306 62-63 0.5259081691554002 2306 64-65 0.4840708490779946 2306 66-67 0.6394627578878698 2306 68-69 0.6838249532244021 2306 70-71 0.46053847887055355 2306 72-73 0.4827199567308895 2306 74-75 0.6252107088909042 2306 76-77 0.2722799130700473 2306 78-79 0.48148958258538954 2306 80-81 0.5958644717096817 2306 82-83 0.5617506019166782 2306 84-85 0.5612389518978631 2306 86-87 0.6874624701994705 2306 88-89 0.6208086159200192 2306 90-91 0.6352767101168126 2306 92-93 0.5166227475181486 2306 94-95 0.5399563103713874 2306 96-97 0.5609328068926871 2306 98-99 0.5692975606853707 2306 100-101 0.5529810295835276 2307 1 0.1097687919070296 2307 2 0.16584011388076192 2307 3 0.10579503872622809 2307 4 0.13813943921596916 2307 5 0.10306631249728326 2307 6 0.12787543502493293 2307 7 0.1541177353689207 2307 8 0.20040057086973917 2307 9 0.17149468121321831 2307 10-11 0.18024855939423645 2307 12-13 0.2173439663295582 2307 14-15 0.3025594722673475 2307 16-17 0.4041957528101676 2307 18-19 0.4010424557278469 2307 20-21 0.34656496246026336 2307 22-23 0.38855020296617937 2307 24-25 0.33284476422755205 2307 26-27 0.3670298631688027 2307 28-29 0.22368713072452096 2307 30-31 0.31373649134081916 2307 32-33 0.4816683793993377 2307 34-35 0.39628254265927154 2307 36-37 0.3850394601801099 2307 38-39 0.3271888125859448 2307 40-41 0.450633295736786 2307 42-43 0.40610644500842596 2307 44-45 0.34176919126205973 2307 46-47 0.3356246727993266 2307 48-49 0.40995172701230587 2307 50-51 0.37890561420245916 2307 52-53 0.3694936964751676 2307 54-55 0.4887241059236871 2307 56-57 0.5648774017828444 2307 58-59 0.3271337547366642 2307 60-61 0.19646915847269497 2307 62-63 0.252420072713214 2307 64-65 0.2383707756807425 2307 66-67 0.3461981434483903 2307 68-69 0.2367129457659516 2307 70-71 0.21950148472026854 2307 72-73 0.25904525750762275 2307 74-75 0.2444704005796936 2307 76-77 -0.06819491913064368 2307 78-79 0.2427139205955342 2307 80-81 0.3372254139942896 2307 82-83 0.2882514721098559 2307 84-85 0.2729005344007902 2307 86-87 0.35116377986786773 2307 88-89 0.3337240214303705 2307 90-91 0.3020644499275278 2307 92-93 0.3036147472334747 2307 94-95 0.2647440169484341 2307 96-97 0.3950806322859819 2307 98-99 0.3064870362553904 2307 100-101 0.15540428772381532 2201 1 0.15871171550742247 2201 2 0.19051924431959577 2201 3 0.14664443831410523 2201 4 0.14962455580006662 2201 5 0.1502576945204126 2201 6 0.11369165979417772 2201 7 0.15868389733757482 2201 8 0.1461902340532788 2201 9 0.17113713250027018 2201 10-11 0.23697928530946655 2201 12-13 0.18183277335291592 2201 14-15 0.31147347850860996 2201 16-17 0.2873431170626901 2201 18-19 0.3376940257330432 2201 20-21 0.39471201926031796 2201 22-23 0.37094497934149473 2201 24-25 0.3493365964486017 2201 26-27 0.35998352851907356 2201 28-29 0.3063510758478927 2201 30-31 0.42647825439107123 2201 32-33 0.4817169852550265 2201 34-35 0.4040749844054474 2201 36-37 0.3190427081666556 2201 38-39 0.43190306135648626 2201 40-41 0.5185787531397636 2201 42-43 0.4297100687264148 2201 44-45 0.3833030391828416 2201 46-47 0.38077677234873875 2201 48-49 0.3412586480871198 2201 50-51 0.45467791245125966 2201 52-53 0.39957348555940797 2201 54-55 0.6557778405865804 2201 56-57 0.4702144930074823 2201 58-59 0.38570200795903986 2201 60-61 0.365922897404829 2201 62-63 0.41681094206236224 2201 64-65 0.4073507156931413 2201 66-67 0.5241395897865431 2201 68-69 0.5105188414783584 2201 70-71 0.38516613037372593 2201 72-73 0.3256486932925853 2201 74-75 0.3596835622569543 2201 76-77 0.15256429568141883 2201 78-79 0.4493838795757483 2201 80-81 0.4114826855096325 2201 82-83 0.4178351401192728 2201 84-85 0.3524242095150285 2201 86-87 0.4247283097663157 2201 88-89 0.4473729976328755 2201 90-91 0.4517239949949925 2201 92-93 0.4275492946170445 2201 94-95 0.34578245437257493 2201 96-97 0.31739472486393794 2201 98-99 0.28578894655206 2201 100-101 0.3331167432700113 1101 1 0.08374935813839812 1101 2 0.15483533123651938 1101 3 0.07700350111016974 1101 4 0.0939300211670755 1101 5 0.09456315988742148 1101 6 0.09020242150597113 1101 7 0.1275170999714348 1101 8 0.146148836030207 1101 9 0.14916542745763195 1101 10-11 0.1989729431323397 1101 12-13 0.1651564668608856 1101 14-15 0.23309282723490554 1101 16-17 0.28208438533252433 1101 18-19 0.33144588125157526 1101 20-21 0.35348313668758635 1101 22-23 0.2689485301090784 1101 24-25 0.2755268783218199 1101 26-27 0.2228555341113534 1101 28-29 0.25294703469115376 1101 30-31 0.4030416507321988 1101 32-33 0.3459634052131335 1101 34-35 0.4073951058554215 1101 36-37 0.35102622938787675 1101 38-39 0.3744112611220558 1101 40-41 0.5354839315593267 1101 42-43 0.3427269082594009 1101 44-45 0.4322958252816207 1101 46-47 0.41614251205452746 1101 48-49 0.4017192779757792 1101 50-51 0.37849135640011156 1101 52-53 0.2966982154373028 1101 54-55 0.5472304499039709 1101 56-57 0.4353313359705808 1101 58-59 0.4090008153411375 1101 60-61 0.44511140152866346 1101 62-63 0.36646207500796635 1101 64-65 0.2034151830602582 1101 66-67 0.4653538056311035 1101 68-69 0.3928922729368409 1101 70-71 0.3238615722148239 1101 72-73 0.21731538952926854 1101 74-75 0.21500515984214275 1101 76-77 0.04308249247815965 1101 78-79 0.345503228893449 1101 80-81 0.48563955562983807 1101 82-83 0.5714253540997376 1101 84-85 0.514669567318137 1101 86-87 0.4434100547757751 1101 88-89 0.3091538696171767 1101 90-91 0.3481236680688795 1101 92-93 0.4007079992610656 1101 94-95 0.32571860679299647 1101 96-97 0.36850294994143695 1101 98-99 0.3724864606599141 1101 100-101 0.2828808336785613 2304 1 0.1305219819612944 2304 2 0.15760738778989491 2304 3 0.11473747271797663 2304 4 0.1093423941079621 2304 5 0.07587125999413047 2304 6 0.11508335065367703 2304 7 0.09734581296987699 2304 8 0.08174365125731953 2304 9 0.1319087467399811 2304 10-11 0.13938942901793894 2304 12-13 0.12236058530823613 2304 14-15 0.17761172981543183 2304 16-17 0.18976840882639578 2304 18-19 0.2869215000923333 2304 20-21 0.3069203111463068 2304 22-23 0.178776498726684 2304 24-25 0.17509330055404604 2304 26-27 0.21427153173767977 2304 28-29 0.24357860492481365 2304 30-31 0.36104190773609446 2304 32-33 0.359181198289221 2304 34-35 0.3298812784601708 2304 36-37 0.3397111595343887 2304 38-39 0.3045897833769118 2304 40-41 0.41626759211140296 2304 42-43 0.33366533505062534 2304 44-45 0.2763536569339635 2304 46-47 0.29621185177028053 2304 48-49 0.2252498056511527 2304 50-51 0.22192286137210004 2304 52-53 0.34467319878699954 2304 54-55 0.41770985040789554 2304 56-57 0.39898399424034636 2304 58-59 0.27084527792986535 2304 60-61 0.3310241908976863 2304 62-63 0.30128692131699353 2304 64-65 0.2838077508989798 2304 66-67 0.30571506054488395 2304 68-69 0.2271532146732369 2304 70-71 0.13681435208015813 2304 72-73 0.1309986171452806 2304 74-75 0.14533027217907346 2304 76-77 -0.023075516630399306 2304 78-79 0.2186777813043932 2304 80-81 0.36531015427966906 2304 82-83 0.25754905671735173 2304 84-85 0.28846364430877003 2304 86-87 0.4262870698279144 2304 88-89 0.3086564860596397 2304 90-91 0.26151349493376586 2304 92-93 0.20334823703940685 2304 94-95 0.2594805847668695 2304 96-97 0.3192826928685548 2304 98-99 0.1917691847600551 2304 100-101 0.21508773352733357 2202 1 0.0889176085095329 2202 2 0.11505787251599742 2202 3 0.0888925393695672 2202 4 0.07766164122606511 2202 5 0.07791869871286394 2202 6 0.052824510349026355 2202 7 0.09451333600392786 2202 8 0.0846993018299429 2202 9 0.09507114026778396 2202 10-11 0.12300026292905386 2202 12-13 0.11584476785638032 2202 14-15 0.15050903988372255 2202 16-17 0.11415289829870545 2202 18-19 0.23002646125394932 2202 20-21 0.22775097301536817 2202 22-23 0.1657174059124671 2202 24-25 0.1513867303072729 2202 26-27 0.016514721401890142 2202 28-29 0.06579998600538062 2202 30-31 0.11284701657335461 2202 32-33 0.112949529300856 2202 34-35 0.07212484258393204 2202 36-37 0.14562298403846796 2202 38-39 0.14024928700570882 2202 40-41 0.24218237543568222 2202 42-43 0.10964483058873498 2202 44-45 0.130533576495921 2202 46-47 0.0567606281499593 2202 48-49 0.06203600501783768 2202 50-51 0.05916456720345309 2202 52-53 0.165208553592592 2202 54-55 0.2371817548104289 2202 56-57 0.3581625950854743 2202 58-59 0.2487825335452527 2202 60-61 0.2035387340076369 2202 62-63 0.1420914108217275 2202 64-65 0.14664196158319953 2202 66-67 0.11737236989962696 2202 68-69 0.12267144237678451 2202 70-71 0.07740265753124476 2202 72-73 0.09952429615177039 2202 74-75 0.03302432220835527 2202 76-77 -0.10291744457229157 2202 78-79 0.22754723924764875 2202 80-81 0.27079678290379405 2202 82-83 0.1912115557277616 2202 84-85 0.15179733097074433 2202 86-87 0.1637750344176041 2202 88-89 0.24547218517341918 2202 90-91 0.11828120277982634 2202 92-93 -0.02018429059651794 2202 94-95 0.1657591069017741 2202 96-97 0.23990286866109756 2202 98-99 0.2620021708435729 2202 100-101 0.3160639230593034 1102 1 0.08191273563198109 1102 2 0.1492334833943545 1102 3 0.07552465445837697 1102 4 0.09865555688158878 1102 5 0.04059383595920707 1102 6 0.020428669974741354 1102 7 0.051491521235796256 1102 8 0.09123225651359235 1102 9 0.10046632239708231 1102 10-11 0.14520177227205977 1102 12-13 0.10122764336828283 1102 14-15 0.1337806915987727 1102 16-17 0.14156545666978815 1102 18-19 0.25880781546722176 1102 20-21 0.2604411795611554 1102 22-23 0.16536514681319403 1102 24-25 0.16606740333774894 1102 26-27 0.12919170272136427 1102 28-29 0.22681214633087876 1102 30-31 0.2102559293009847 1102 32-33 0.20264414941973286 1102 34-35 0.23264427890904216 1102 36-37 0.2678297102575016 1102 38-39 0.2074049461096621 1102 40-41 0.3190795393608994 1102 42-43 0.19979008322469838 1102 44-45 0.15320100565369188 1102 46-47 0.05571228285940322 1102 48-49 0.23931518685811426 1102 50-51 0.24653545637712426 1102 52-53 0.29506636664251573 1102 54-55 0.3555156875246581 1102 56-57 0.37972464293621755 1102 58-59 0.29849596869426875 1102 60-61 0.34804767378624035 1102 62-63 0.19048715631880242 1102 64-65 0.15871837832836633 1102 66-67 0.2943183160716103 1102 68-69 0.28070072872596796 1102 70-71 0.24517660213528103 1102 72-73 0.09994812448323387 1102 74-75 0.16321643349658643 1102 76-77 -0.0769417208010097 1102 78-79 0.2634419170332336 1102 80-81 0.3664452913228544 1102 82-83 0.3911006165625679 1102 84-85 0.3895256755538341 1102 86-87 0.431054273842328 1102 88-89 0.2715574907623832 1102 90-91 0.2670660271411194 1102 92-93 0.18173897484704327 1102 94-95 0.18639407954984932 1102 96-97 0.4043422286127978 1102 98-99 0.5005751214853476 1102 100-101 0.3450116208951002 2305 1 0.07683676408606033 2305 2 0.14593905240499083 2305 3 0.0881803006003068 2305 4 0.0629551482753925 2305 5 0.05261871870132495 2305 6 0.018818768788968043 2305 7 0.06393850395627254 2305 8 0.10323308007296106 2305 9 0.04451562862829661 2305 10-11 0.14396772325221718 2305 12-13 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-1.2841222859909962 1213 94-95 -1.334324165223709 1213 96-97 -1.4983827267545777 1213 98-99 -1.5328096749580702 1213 100-101 -1.4087341961979902 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 9.0 8 74.0 9 230.0 10 322.0 11 208.0 12 249.0 13 523.0 14 1310.0 15 2778.0 16 4815.0 17 7305.0 18 9565.0 19 11792.0 20 14348.0 21 17405.0 22 21565.0 23 26010.0 24 31532.0 25 38044.0 26 46499.0 27 57843.0 28 73583.0 29 92109.0 30 107470.0 31 133479.0 32 174948.0 33 243269.0 34 367473.0 35 633826.0 36 1256385.0 37 2028486.0 38 499925.0 39 202.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.12483235527499 28.979578263980233 16.41280243278051 16.482786947964264 2 33.40519366039127 23.52819017335577 28.34099704213476 14.725619124118206 3 39.2794897520667 20.540907799683676 21.81613803241239 18.363464415837228 4 33.30459050619337 20.54589235636182 27.256941562161924 18.892575575282887 5 35.12599638660055 18.48476060805118 26.948910000741922 19.440333004606355 6 32.167301140223 19.47282175465675 28.71897095695461 19.640906148165637 7 35.97537901565524 20.004346513692873 22.22850466039093 21.79176981026096 8 31.079486996199932 26.00942952939419 22.15805590571961 20.753027568686267 9 29.43968593982433 22.552714606149284 26.845870862808376 21.161728591218008 10-11 22.642863265048238 30.15873244413307 31.347790206013908 15.850614084804784 12-13 22.505048629797976 29.327880937369887 31.508802621865843 16.65826781096629 14-15 22.403584806647896 28.996709116600734 31.8065371159408 16.793168960810572 16-17 22.133562301667023 28.925743726435915 31.817581258293693 17.12311271360337 18-19 21.061636816427722 29.266486007986337 31.82085892937542 17.851018246210522 20-21 21.619969166174805 29.048967986636587 31.214348442551355 18.116714404637253 22-23 20.981042510328905 28.56356409004236 31.493618191140726 18.961775208488007 24-25 20.537395770906546 27.794472914918433 32.166606646608784 19.501524667566233 26-27 20.4977080016844 28.257734033337726 31.612968194563912 19.631589770413964 28-29 20.689498341854147 28.76567141779347 30.947956681214894 19.59687355913749 30-31 19.784945817640885 30.45019108737712 30.464351981559666 19.300511113422324 32-33 19.412646423815236 31.167577582559197 31.000255438139085 18.419520555486482 34-35 20.014145649200774 30.33061114421719 32.13032358998317 17.524919616598872 36-37 19.658684219084968 29.93998003908166 33.15011545622827 17.2512202856051 38-39 20.195164547012947 29.43670512985561 31.73341825499536 18.634712068136082 40-41 20.393706022728956 29.177213447648654 31.28514952536839 19.143931004254004 42-43 20.40182053437903 28.836550402930904 31.360480764450305 19.40114829823976 44-45 20.018797302924966 28.325284303489802 31.795391433887637 19.860526959697598 46-47 20.72036393227358 28.622891849535094 30.978479604357812 19.67826461383352 48-49 20.44662070500577 29.808397654334197 30.404232409518013 19.340749231142027 50-51 21.452982969006175 29.59167149287435 28.866114195047 20.089231343072477 52-53 21.08383125291073 29.544336033158913 28.7567763171158 20.61505639681456 54-55 21.720700890930598 29.434206631581567 29.02434772030171 19.82074475718613 56-57 21.224236887291074 30.08397569208529 28.954229672721503 19.73755774790213 58-59 20.78975046921925 31.044491032228137 28.724613112251067 19.441145386301542 60-61 20.990167993762576 31.008549684829017 28.476117399168206 19.5251649222402 62-63 20.412749294053466 30.74791328734563 29.226936136560155 19.612401282040747 64-65 20.810485367617552 29.645920552860773 29.856814426774054 19.686779652747628 66-67 21.08581251507452 29.651636866953957 29.218888633134533 20.043661984836994 68-69 21.22217783077642 30.34107032181087 29.418092530071817 19.01865931734089 70-71 20.550351129330743 30.942655935954605 29.540282021764835 18.966710912949818 72-73 20.349153481186256 30.332694911042644 29.61762353903397 19.700528068737132 74-75 20.60570891356241 29.38889400072831 29.295271085363257 20.710126000346023 76-77 20.598025809868442 28.71487212728364 28.87710926491634 21.809992797931578 78-79 19.91106865421629 29.284079457826433 29.29958010195019 21.505271786007086 80-81 19.48402151697667 29.873906599840616 28.932945464845844 21.709126418336865 82-83 18.64240235582478 30.104661820275613 28.986048224547023 22.266887599352582 84-85 18.36870910630045 30.47681865207556 30.020572740069085 21.133899501554897 86-87 18.80578732673925 30.178565195451988 30.60230233761586 20.413345140192902 88-89 19.623583070310307 29.947607727908842 30.078158518917707 20.35065068286314 90-91 19.534902869759605 30.170761399870916 29.473125022819268 20.82121070755021 92-93 18.938181519913915 30.572502360087146 29.682892920721493 20.806423199277447 94-95 19.084444470808464 29.99987712483989 29.810225670824224 21.105452733527418 96-97 19.41671186188218 30.684067608370867 29.343326580810867 20.555893948936085 98-99 20.216914255639853 30.4825165801823 28.709066781371025 20.591502382806816 100-101 19.902805112898402 30.943837588292556 27.956153854626205 21.197203444182836 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11118.0 1 6366.5 2 1713.0 3 1741.5 4 1376.0 5 956.0 6 847.0 7 893.0 8 909.5 9 893.5 10 858.0 11 826.5 12 777.0 13 743.5 14 767.5 15 798.0 16 810.5 17 869.5 18 986.5 19 1166.0 20 1491.5 21 1912.5 22 2529.5 23 3496.0 24 5106.5 25 7163.0 26 9690.5 27 13265.0 28 17865.5 29 23514.5 30 30915.0 31 39908.5 32 56487.5 33 90293.5 34 137801.0 35 181530.0 36 237912.0 37 322671.5 38 401806.5 39 444110.0 40 447388.5 41 436919.5 42 438343.0 43 433504.5 44 385234.5 45 318902.0 46 275011.5 47 239870.0 48 202882.5 49 162976.5 50 120033.0 51 90369.5 52 73317.0 53 56218.0 54 40220.5 55 29163.0 56 22042.0 57 16874.0 58 12744.0 59 9535.0 60 7128.0 61 5607.0 62 4501.5 63 3470.0 64 2553.0 65 1924.0 66 1530.5 67 1231.0 68 1011.5 69 852.0 70 707.5 71 556.0 72 415.5 73 314.0 74 238.5 75 183.0 76 137.0 77 103.0 78 74.0 79 54.5 80 35.5 81 22.0 82 15.5 83 12.0 84 9.5 85 5.5 86 1.5 87 1.5 88 2.5 89 3.0 90 1.5 91 0.0 92 1.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15958108145190497 2 0.0 3 0.0016769479953018353 4 0.011179653302012236 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.469434319706239E-6 22-23 8.469434319706239E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.79187957412974E-4 38-39 0.0017277646012200728 40-41 0.005411968530292287 42-43 0.002549299730231578 44-45 0.0012788845822756423 46-47 0.0011179653302012236 48-49 0.0031082823953321897 50-51 8.638823006100364E-4 52-53 0.014482732686697669 54-55 0.026272185259728752 56-57 0.027711989094078814 58-59 0.020733175214640874 60-61 0.03024434995567098 62-63 0.005716868165801712 64-65 0.010383526475959848 66-67 0.02797454155798971 68-69 0.02615361317925287 70-71 0.02994791975448126 72-73 0.03037986090478628 74-75 0.038095515570038666 76-77 0.018819083058387264 78-79 0.010188729486606606 80-81 0.015143348563634756 82-83 0.007224427474709422 84-85 0.0105867928996328 86-87 0.03265813873678726 88-89 0.015219573472512111 90-91 0.02072470578032117 92-93 0.04821648958208762 94-95 0.05563571404615028 96-97 0.03947603336415079 98-99 0.034445189378245276 100-101 0.03450447541848322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5903582.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.512319571366966 #Duplication Level Percentage of deduplicated Percentage of total 1 80.27664850914807 28.50809995975166 2 9.508544310405295 6.753409284192319 3 2.941370812311819 3.1336470079412573 4 1.8461885599724774 2.622497525229776 5 1.0962519656702137 1.9465225067809917 6 0.5752579838277391 1.2257247214602554 7 0.41855916567561424 1.0404804795698002 8 0.40124530696810984 1.1399321254050203 9 0.3260490544028267 1.0420882394305675 >10 2.1217117445903244 14.728109053625497 >50 0.24364364906078537 6.009097059876763 >100 0.20246627250354038 15.203925254303236 >500 0.027358287069959762 6.653977587616739 >1k 0.01441958197618503 8.587509336982645 >5k 1.4240007745537257E-4 0.3614704473582016 >10k+ 1.423963396510252E-4 1.0435094104752118 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 27786 0.4706634040147152 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 19363 0.3279873134649438 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14758 0.24998382338044933 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7660 0.12975173377789959 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7538 0.12768519180389126 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6246 0.10580017352177033 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.420437964611993E-4 0.0 0.0 2 6.097992710188492E-4 0.0 0.0 3 7.622490887735615E-4 0.0 0.0 4 8.638823006100364E-4 0.0 0.0 5 9.824543810859237E-4 0.0 0.0 6 0.0011518430674800485 0.0 0.0 7 0.0013720483597924108 0.0 0.0 8 0.0017616423384988979 0.0 0.0 9 0.002066541974008322 0.0 0.0 10-11 0.0030320574864548338 0.0 0.0 12-13 0.0047174749160763755 0.0 0.0 14-15 0.007893512785966216 0.0 0.0 16-17 0.014203241354147363 0.0 0.0 18-19 0.02112276919334736 0.0 0.0 20-21 0.028203216284621777 0.0 0.0 22-23 0.03602897359603034 0.0 0.0 24-25 0.0446508577334913 0.0 0.0 26-27 0.054915812128975255 0.0 0.0 28-29 0.06723883906414783 0.0 0.0 30-31 0.08018860413897867 0.0 0.0 32-33 0.09400225151441954 0.0 0.0 34-35 0.10881529213958577 0.0 0.0 36-37 0.12448374563104231 0.0 0.0 38-39 0.14165975843140655 0.0 0.0 40-41 0.1585816882021796 0.0 0.0 42-43 0.17829006186413604 0.0 0.0 44-45 0.19849813215095513 0.0 0.0 46-47 0.21945998209222806 0.0 0.0 48-49 0.24244602683591082 0.0 0.0 50-51 0.2665415674754751 0.0 0.0 52-53 0.2912977239919764 0.0 0.0 54-55 0.31812889191680577 0.0 0.0 56-57 0.3465607829280596 0.0 0.0 58-59 0.37592431171448115 0.0 0.0 60-61 0.4092091885909267 0.0 0.0 62-63 0.44475540443073375 0.0 0.0 64-65 0.4847565427227063 0.0 0.0 66-67 0.5282216796514387 0.0 0.0 68-69 0.5782675670465829 0.0 0.0 70-71 0.6341150169507259 0.0 0.0 72-73 0.6971021999863811 0.0 0.0 74-75 0.7707015842246283 0.0 0.0 76-77 0.8618242280703479 0.0 0.0 78-79 0.97189299648925 0.0 1.6938868639412477E-5 80-81 1.1072091486151967 0.0 3.3877737278824954E-5 82-83 1.280239691766795 0.0 3.3877737278824954E-5 84-85 1.4951600570636607 0.0 3.3877737278824954E-5 86-87 1.7477270579116204 0.0 3.3877737278824954E-5 88-89 2.041938267309576 0.0 3.3877737278824954E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGGG 255 0.0 59.700993 1 CGGACGG 85 1.8189894E-12 55.96968 1 CGCGGAG 395 0.0 55.4029 1 CGATTCG 60 1.4773632E-6 47.574226 1 CCGAGTG 130 0.0 43.914677 1 TGTACCG 175 0.0 43.425694 2 CAACGGG 175 0.0 40.777912 1 CACGGGG 590 0.0 40.317142 1 CGAGTGG 285 0.0 40.06251 1 CAAGGCG 190 0.0 40.062508 1 GGACGGG 755 0.0 39.6977 1 CGGAGCC 195 0.0 39.035267 1 CGCGGGG 500 0.0 39.010868 1 CGAACGG 75 6.853312E-6 38.059383 1 GTTACGG 330 0.0 37.482727 1 ACGCGGA 90 5.633974E-7 37.002174 1 CGGACCG 260 0.0 36.595562 1 CGAGGGG 885 0.0 36.016647 1 ATGACCG 160 1.8189894E-12 35.68067 1 TGCGGCC 365 0.0 35.13466 2 >>END_MODULE