FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_44065-1.341000000357de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_44065-1.341000000357de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3840504
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT183560.47795810133253347TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCG156.6233345E-494.984772
CGTGCGC250.00499151357.088241
CGAGGCG1900.052.5812721
CGGGTCG558.1088365E-751.89841
GGTACGG953.8016879E-1045.0696641
CGCGAGG857.1304385E-944.775091
CGCGCCC751.3474346E-744.4019661
CGGCGAG652.5635381E-643.914031
CGGTGCG459.554877E-442.2875861
GGCCCGC951.9041181E-840.0619241
CGAGCGG951.9041181E-840.0619241
CGCCGAG853.5997436E-739.1782041
CGTGCGG500.00160405138.0588261
CGCGCAA500.00160405138.0588261
GTACGCA500.00160405138.0588261
CGCGATA756.934133E-637.993913
ACGGCGT651.3304249E-436.5307044
CGAGCCC2250.035.9444471
CGAGTGG2150.035.403561
CGAGGGG4450.035.2792471
Produced by FastQC (version 0.11.2)