##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_41442-1.34100000035721.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2995253 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.680674053243585 33.0 33.0 33.0 33.0 33.0 2 32.65400051347916 33.0 33.0 33.0 33.0 33.0 3 32.73004450709172 33.0 33.0 33.0 33.0 33.0 4 36.672862359206384 37.0 37.0 37.0 37.0 37.0 5 36.68489506562551 37.0 37.0 37.0 37.0 37.0 6 36.577174949828944 37.0 37.0 37.0 37.0 37.0 7 36.4599684901409 37.0 37.0 37.0 37.0 37.0 8 36.41963934265319 37.0 37.0 37.0 37.0 37.0 9 36.45384914062351 37.0 37.0 37.0 37.0 37.0 10-11 36.310438383669094 37.0 37.0 37.0 37.0 37.0 12-13 36.35306633529789 37.0 37.0 37.0 37.0 37.0 14-15 38.24753050910891 40.0 37.0 40.0 37.0 40.0 16-17 38.26448750739921 40.0 37.0 40.0 37.0 40.0 18-19 38.14940774619039 40.0 37.0 40.0 37.0 40.0 20-21 38.18620914493701 40.0 37.0 40.0 37.0 40.0 22-23 37.98824239555056 40.0 37.0 40.0 33.0 40.0 24-25 38.078662971041176 40.0 37.0 40.0 37.0 40.0 26-27 38.02952388329133 40.0 37.0 40.0 37.0 40.0 28-29 37.81010468898621 40.0 37.0 40.0 33.0 40.0 30-31 37.72176023194034 40.0 37.0 40.0 33.0 40.0 32-33 37.625004799260694 40.0 37.0 40.0 33.0 40.0 34-35 37.63918590516394 40.0 37.0 40.0 33.0 40.0 36-37 37.57977372862993 40.0 37.0 40.0 33.0 40.0 38-39 37.2821613065741 40.0 37.0 40.0 33.0 40.0 40-41 37.06820225203013 40.0 37.0 40.0 33.0 40.0 42-43 36.94081368084766 37.0 37.0 40.0 33.0 40.0 44-45 36.76340729814811 37.0 37.0 40.0 33.0 40.0 46-47 36.89435333175528 37.0 37.0 40.0 33.0 40.0 48-49 36.5521904159682 37.0 37.0 40.0 33.0 40.0 50-51 36.495420921037386 37.0 37.0 40.0 33.0 40.0 52-53 36.4421361066995 37.0 37.0 40.0 33.0 40.0 54-55 35.87686899904615 37.0 37.0 40.0 30.0 40.0 56-57 35.78313100763108 37.0 37.0 40.0 27.0 40.0 58-59 35.69412500379768 37.0 37.0 40.0 27.0 40.0 60-61 35.310952697484986 37.0 33.0 40.0 27.0 40.0 62-63 35.09578072369847 37.0 33.0 38.5 27.0 40.0 64-65 34.865665604875446 37.0 33.0 37.0 27.0 40.0 66-67 34.03349166163927 37.0 33.0 37.0 27.0 40.0 68-69 33.98627711916156 37.0 33.0 37.0 27.0 40.0 70-71 33.691603013167835 37.0 33.0 37.0 27.0 38.5 72-73 32.88695495839583 37.0 33.0 37.0 22.0 37.0 74-75 31.951140855213232 37.0 33.0 37.0 18.5 37.0 76-77 29.432544262538087 33.0 27.0 35.0 15.0 37.0 78-79 31.44668046405429 33.0 33.0 37.0 15.0 37.0 80-81 31.72324508146724 37.0 33.0 37.0 15.0 37.0 82-83 31.594104905328532 37.0 33.0 37.0 15.0 37.0 84-85 31.467011801674182 37.0 33.0 37.0 15.0 37.0 86-87 31.34591618804822 35.0 33.0 37.0 15.0 37.0 88-89 31.192585734827745 33.0 33.0 37.0 15.0 37.0 90-91 31.08947558019306 33.0 33.0 37.0 15.0 37.0 92-93 30.92149611401775 33.0 33.0 37.0 15.0 37.0 94-95 30.80875772430576 33.0 33.0 37.0 15.0 37.0 96-97 30.6545622356442 33.0 33.0 37.0 15.0 37.0 98-99 30.53087852678889 33.0 33.0 37.0 15.0 37.0 100-101 29.032442501518236 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16907439315184547 2306 2 0.21583682372496327 2306 3 0.14778614917986488 2306 4 0.1842770479293776 2306 5 0.15046126130180681 2306 6 0.20066645749755452 2306 7 0.08643014231657986 2306 8 0.20951918013633275 2306 9 0.15093582380519166 2306 10-11 0.19975932792574724 2306 12-13 0.24154653784643187 2306 14-15 0.3210324866487966 2306 16-17 0.3459305184365178 2306 18-19 0.48194982457818725 2306 20-21 0.5608552540794065 2306 22-23 0.4736982185014469 2306 24-25 0.45006948793498935 2306 26-27 0.5386261001460255 2306 28-29 0.5093191721479542 2306 30-31 0.6715073493767747 2306 32-33 0.6047387686033758 2306 34-35 0.563023840309782 2306 36-37 0.6012030050525041 2306 38-39 0.5820230506911628 2306 40-41 0.6624077762281573 2306 42-43 0.5382790003377877 2306 44-45 0.6053933288946283 2306 46-47 0.5953681132664528 2306 48-49 0.32574984802776186 2306 50-51 0.4050390734561873 2306 52-53 0.6562195152328201 2306 54-55 0.5684111129204013 2306 56-57 0.5857961380560894 2306 58-59 0.4607578321897563 2306 60-61 0.3599951127281287 2306 62-63 0.33240924502914737 2306 64-65 0.4850066358212075 2306 66-67 0.2665667269360128 2306 68-69 0.28643068319257026 2306 70-71 0.4302010949567787 2306 72-73 0.045570944323166884 2306 74-75 0.07976946479815439 2306 76-77 -0.13489665314657984 2306 78-79 0.05398001578982203 2306 80-81 0.14693872891160353 2306 82-83 0.12955296719093212 2306 84-85 0.1141354025511987 2306 86-87 0.23857462303915966 2306 88-89 0.2142523210566516 2306 90-91 0.2662490095699823 2306 92-93 0.22850039437794223 2306 94-95 0.1123267224797786 2306 96-97 0.1195522087259242 2306 98-99 0.23934405666615532 2306 100-101 0.027910440719910667 2307 1 0.14699907263902645 2307 2 0.17773586218650195 2307 3 0.11204896969267963 2307 4 0.1171696761345089 2307 5 0.11869042796847395 2307 6 0.14678023954883912 2307 7 0.184667321803758 2307 8 0.16448712885427597 2307 9 0.10065537508724276 2307 10-11 0.1315702253616422 2307 12-13 0.19326929425668737 2307 14-15 0.258412294341106 2307 16-17 0.3762790761288244 2307 18-19 0.4082118438089566 2307 20-21 0.37758201689992177 2307 22-23 0.35006039798862076 2307 24-25 0.4114276610119134 2307 26-27 0.3932535039921774 2307 28-29 0.4455571529171891 2307 30-31 0.4160986955306285 2307 32-33 0.4395344416803013 2307 34-35 0.31883313518157763 2307 36-37 0.3814113383858384 2307 38-39 0.4951240122296241 2307 40-41 0.5659133852025207 2307 42-43 0.560594705465995 2307 44-45 0.4331377199202677 2307 46-47 0.28953878634337116 2307 48-49 0.44139488007904504 2307 50-51 0.5369501311484939 2307 52-53 0.4463236818994858 2307 54-55 0.4043886770229719 2307 56-57 0.5209924521586586 2307 58-59 0.3238788257795022 2307 60-61 0.16306001657428482 2307 62-63 0.17778184118299123 2307 64-65 0.15545936018018125 2307 66-67 0.14441127821805821 2307 68-69 0.24211978575667104 2307 70-71 0.1692435629054927 2307 72-73 0.12996597637445007 2307 74-75 0.1654465481314844 2307 76-77 -0.14377085186452732 2307 78-79 0.21503770809751543 2307 80-81 0.33956693403980864 2307 82-83 0.369456813344776 2307 84-85 0.40143508203837897 2307 86-87 0.3727292704750589 2307 88-89 0.5208228338771654 2307 90-91 0.43952625315972327 2307 92-93 0.39382491360871086 2307 94-95 0.2812970750438808 2307 96-97 0.4997405100079746 2307 98-99 0.4907262682046145 2307 100-101 0.09966524841221869 2201 1 0.03861328517592 2201 2 0.17320491063241406 2201 3 0.07181737566594393 2201 4 0.13865886696624585 2201 5 0.049968644672766516 2201 6 0.055746639965576605 2201 7 0.12893390454382114 2201 8 0.11388806651707029 2201 9 0.18105127715357838 2201 10-11 0.1427310171657723 2201 12-13 0.09309022671023826 2201 14-15 0.24411727784518433 2201 16-17 0.27763727633719526 2201 18-19 0.27297514907028386 2201 20-21 0.353514796028243 2201 22-23 0.3230022064623128 2201 24-25 0.26288516231422676 2201 26-27 0.24719516052977042 2201 28-29 0.3404721772269568 2201 30-31 0.4299688335754226 2201 32-33 0.40078313937087273 2201 34-35 0.3662887481352115 2201 36-37 0.31845987111721996 2201 38-39 0.37217189380629634 2201 40-41 0.5280004445878319 2201 42-43 0.3457787651985882 2201 44-45 0.5483150510541392 2201 46-47 0.4035103959978201 2201 48-49 0.2821615062294214 2201 50-51 0.2981630153297701 2201 52-53 0.4142836141793964 2201 54-55 0.4889199541544116 2201 56-57 0.5527796547980017 2201 58-59 0.3590113357638671 2201 60-61 0.35059365956786337 2201 62-63 0.1966745525912259 2201 64-65 0.19480429853753378 2201 66-67 0.3116723044378844 2201 68-69 0.4252874363904624 2201 70-71 0.29580904736460667 2201 72-73 0.20323824586568406 2201 74-75 0.2533204194633143 2201 76-77 0.04222606600315615 2201 78-79 0.49619220540607145 2201 80-81 0.6476417951268019 2201 82-83 0.5678924141435289 2201 84-85 0.5422592268551867 2201 86-87 0.6069854347396877 2201 88-89 0.5847494053305446 2201 90-91 0.581691776688114 2201 92-93 0.4358173385088655 2201 94-95 0.5154523103277846 2201 96-97 0.47934634435421586 2201 98-99 0.42912431908150594 2201 100-101 0.4011715863181067 1101 1 0.1540693878632382 1101 2 0.1929517942598693 1101 3 0.15675789290908426 1101 4 0.13528329981816967 1101 5 0.1387550245233271 1101 6 0.09516488980339233 1101 7 0.1350966578801973 1101 8 0.14611067303448522 1101 9 0.17016510379914962 1101 10-11 0.21804940801035855 1101 12-13 0.20525016809725827 1101 14-15 0.3058197146632935 1101 16-17 0.3647534728789452 1101 18-19 0.3892728923479112 1101 20-21 0.30866921789022683 1101 22-23 0.2556942898220811 1101 24-25 0.33077480865122055 1101 26-27 0.33795676084411497 1101 28-29 0.40084016602828143 1101 30-31 0.44816617757260957 1101 32-33 0.3919618250403687 1101 34-35 0.39074605623603986 1101 36-37 0.41659946168592654 1101 38-39 0.5481991046349606 1101 40-41 0.5873494598939146 1101 42-43 0.529182124393401 1101 44-45 0.45917727986168444 1101 46-47 0.4833362966300001 1101 48-49 0.4466584967887215 1101 50-51 0.5089722386873703 1101 52-53 0.40682345524490415 1101 54-55 0.5525547363715972 1101 56-57 0.5245953882071959 1101 58-59 0.48560318765968447 1101 60-61 0.5802984048858804 1101 62-63 0.4991738925812754 1101 64-65 0.3061872700762649 1101 66-67 0.6136546878003131 1101 68-69 0.5540880192457607 1101 70-71 0.40569855453670556 1101 72-73 0.278701941225556 1101 74-75 0.3615622552937694 1101 76-77 0.0550283147440247 1101 78-79 0.4347143766239121 1101 80-81 0.5701472133479868 1101 82-83 0.6175827722679941 1101 84-85 0.7185235296288681 1101 86-87 0.6447988883005742 1101 88-89 0.5338739967895023 1101 90-91 0.5414619704342911 1101 92-93 0.5110713655928087 1101 94-95 0.5161914191404016 1101 96-97 0.6910090782484382 1101 98-99 0.4850213288783074 1101 100-101 0.4462113813364148 2304 1 0.10815454851907447 2304 2 0.1740251308274665 2304 3 0.11837735176258946 2304 4 0.11132633162591077 2304 5 0.08647190496605361 2304 6 0.06419425208996898 2304 7 0.0984660583779231 2304 8 0.050716718473701405 2304 9 0.04590278304651463 2304 10-11 0.1005790414043588 2304 12-13 0.14764898942028282 2304 14-15 0.19166026106364598 2304 16-17 0.19513791855758456 2304 18-19 0.3560891254256475 2304 20-21 0.27975091085907167 2304 22-23 0.25406545013985493 2304 24-25 0.11074278797534731 2304 26-27 0.1848068658845179 2304 28-29 0.2753130432133304 2304 30-31 0.3744267904582941 2304 32-33 0.391543273244217 2304 34-35 0.2608232248941249 2304 36-37 0.3154811527522625 2304 38-39 0.2534554054127156 2304 40-41 0.459307367632519 2304 42-43 0.36036964803755467 2304 44-45 0.39361026291399526 2304 46-47 0.3637278035408613 2304 48-49 0.3721967053723958 2304 50-51 0.2860173069105869 2304 52-53 0.4015143598655868 2304 54-55 0.489592509207732 2304 56-57 0.5417668049245279 2304 58-59 0.37318910236731995 2304 60-61 0.4429557917031133 2304 62-63 0.36018176823333903 2304 64-65 0.32721620503023985 2304 66-67 0.20883241499088712 2304 68-69 0.22475544106182355 2304 70-71 0.332824808637163 2304 72-73 0.06561041667990963 2304 74-75 0.208861096154088 2304 76-77 0.14450016384293107 2304 78-79 0.3452229536429279 2304 80-81 0.5071006538510687 2304 82-83 0.3207732052861729 2304 84-85 0.2799432617118143 2304 86-87 0.3306858266307664 2304 88-89 0.3163785317104022 2304 90-91 0.2823216992390698 2304 92-93 0.10018686027786217 2304 94-95 0.2613802686863984 2304 96-97 0.45842617462584556 2304 98-99 0.3482026371746336 2304 100-101 0.262211035958007 2202 1 0.055613463567247834 2202 2 0.07504560032070628 2202 3 0.05818381888604307 2202 4 0.05280066748358081 2202 5 0.04503921216300455 2202 6 0.048910821732611964 2202 7 0.03841849084748361 2202 8 0.10951284781616977 2202 9 0.038456403845721354 2202 10-11 0.16778427726718093 2202 12-13 0.08035574503994525 2202 14-15 0.06686740306216876 2202 16-17 0.09674976235749 2202 18-19 0.23253160649307603 2202 20-21 0.10803341390717236 2202 22-23 0.021507235725358953 2202 24-25 0.12784541878605893 2202 26-27 0.016497015799522785 2202 28-29 0.1158802473759124 2202 30-31 0.15230617156522186 2202 32-33 0.22194764018392021 2202 34-35 0.11889231802001632 2202 36-37 0.2349447730362968 2202 38-39 0.13801184248448095 2202 40-41 0.28915470505288 2202 42-43 0.08676082657893147 2202 44-45 0.21342033624011947 2202 46-47 0.20532964442146096 2202 48-49 0.24272758992239574 2202 50-51 0.24525326418567062 2202 52-53 0.18600833235541359 2202 54-55 0.4039290967096676 2202 56-57 0.4149572639426182 2202 58-59 0.23687786131841904 2202 60-61 0.3746203976825342 2202 62-63 0.25973399806866126 2202 64-65 0.29127009200155385 2202 66-67 0.2768361671589119 2202 68-69 0.12885406211861294 2202 70-71 0.0215107770743046 2202 72-73 0.010895950655488207 2202 74-75 -0.031903375880673934 2202 76-77 -0.01218182918507793 2202 78-79 0.24748305530349768 2202 80-81 0.21013845028952005 2202 82-83 0.19855942615749456 2202 84-85 0.26267105351371356 2202 86-87 0.2647373953289289 2202 88-89 0.21033578103638817 2202 90-91 0.20255378413938274 2202 92-93 0.15777233586730333 2202 94-95 0.3565571872720774 2202 96-97 0.5143652469731386 2202 98-99 0.3273735969397116 2202 100-101 0.16260363980805792 1102 1 0.11828560959698109 1102 2 0.16179680222379034 1102 3 0.1252913615853899 1102 4 0.1598190566601616 1102 5 0.09783917699115108 1102 6 0.023684521740456432 1102 7 0.035801395900108446 1102 8 0.11634254474409289 1102 9 0.1513019138495011 1102 10-11 0.17134438786840178 1102 12-13 0.10066051035099122 1102 14-15 0.11923054176998704 1102 16-17 0.1429979294998489 1102 18-19 0.2825478259464447 1102 20-21 0.21872048896343443 1102 22-23 0.15368909679177278 1102 24-25 0.10339667225867544 1102 26-27 0.10871931261670653 1102 28-29 0.19963203864521972 1102 30-31 0.275088027641047 1102 32-33 0.27973449280197116 1102 34-35 0.22230395824045956 1102 36-37 0.30601063471109313 1102 38-39 0.21276361949492184 1102 40-41 0.3994535232310241 1102 42-43 0.16312222095285733 1102 44-45 0.24742058814664603 1102 46-47 0.2817179226878679 1102 48-49 0.3689930637562 1102 50-51 0.3184485974984099 1102 52-53 0.2318836897180887 1102 54-55 0.41198193648047976 1102 56-57 0.45102267408684327 1102 58-59 0.38282605769144595 1102 60-61 0.31230709963196546 1102 62-63 0.178385715904831 1102 64-65 0.1630817514714522 1102 66-67 0.45905801244552435 1102 68-69 0.3572224801693764 1102 70-71 0.14591873732060634 1102 72-73 0.14728269015324003 1102 74-75 0.15502092264282652 1102 76-77 0.005395037953238813 1102 78-79 0.38458986723626154 1102 80-81 0.4786684331075861 1102 82-83 0.2955395778242398 1102 84-85 0.43593506700259255 1102 86-87 0.5392088669145849 1102 88-89 0.318494730491107 1102 90-91 0.4087573982851218 1102 92-93 0.28849905055464475 1102 94-95 0.2647246326309407 1102 96-97 0.40370995729250936 1102 98-99 0.6186204689022787 1102 100-101 0.5596625419708907 2305 1 0.04995550768025936 2305 2 0.12107353286380373 2305 3 0.08182979299942872 2305 4 0.050523569604834506 2305 5 0.07197469861873884 2305 6 -0.016649191301503663 2305 7 -0.028061656160282666 2305 8 0.003935553891452059 2305 9 0.025706071315447332 2305 10-11 0.11174124063810353 2305 12-13 0.07525607943986756 2305 14-15 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pass #Quality Count 8 7.0 9 17.0 10 20.0 11 24.0 12 41.0 13 102.0 14 400.0 15 1117.0 16 2395.0 17 4028.0 18 4977.0 19 5997.0 20 7659.0 21 10224.0 22 13354.0 23 17849.0 24 23131.0 25 30045.0 26 40863.0 27 60514.0 28 95701.0 29 111458.0 30 70809.0 31 61054.0 32 79751.0 33 112442.0 34 174235.0 35 304265.0 36 613235.0 37 940889.0 38 208490.0 39 160.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.70735349884597 23.749041093691584 21.91727088074917 13.626334526713274 2 27.844993394547974 28.97995595029869 30.494886408593864 12.680164246559473 3 34.42001356833983 17.74193979182748 32.4637554386874 15.374291201145295 4 27.309862771371208 17.471065257306602 30.950750418457336 24.26832155286486 5 36.481091914439276 16.188382083249728 31.608815682681897 15.721710319629093 6 31.991955270556442 17.059360261053072 35.17963257193967 15.769051896450817 7 30.27323568326282 26.180693250286367 26.462806313857296 17.08326475259352 8 27.00382905884745 33.676120180832804 22.854062745283954 16.465988015035794 9 34.847006246216935 23.453544658831824 23.88936760934719 17.810081485604055 10-11 26.436431246375513 29.943998052919067 27.065860546671683 16.553710154033734 12-13 21.937779546502416 28.616731207680957 27.029436244617735 22.416053001198897 14-15 21.061225879750392 29.199102713527036 27.907759377922332 21.83191202880024 16-17 24.668867704998544 25.675109915589765 28.455309117460192 21.200713261951496 18-19 19.564190403949183 25.756138129233157 32.97155532437493 21.708116142442726 20-21 24.16710708577873 26.010140044931095 32.43393796784445 17.38881490144572 22-23 19.642076442071502 34.48979275008401 28.2958826287702 17.57224817907429 24-25 19.99355313223958 25.3221514175931 32.64707522202632 22.037220228140995 26-27 19.833533260796333 25.484675251139056 28.120913325184883 26.56087816287973 28-29 23.71510854007992 30.198267057907962 27.8185014755014 18.268122926510717 30-31 18.886768496684585 26.510031039114224 31.974093674223848 22.629106789977342 32-33 18.543825847098727 30.93726973981831 28.33104582484351 22.187858588239457 34-35 18.960518527149457 26.543166804273294 32.729188485914214 21.767126182663034 36-37 23.367211025643893 27.139535438866158 28.25974785622032 21.233505679269626 38-39 24.00550079944551 31.244831315160486 27.305846919789357 17.443820965604644 40-41 23.75050019390078 30.924144645475415 27.16899684266425 18.156358317959555 42-43 19.038436048815715 30.983782960840788 32.0836322855732 17.894148704770295 44-45 18.728070270619476 26.66778622809239 32.53132782711657 22.07281567417157 46-47 23.509783424851406 26.28176771047459 27.99975561052464 22.208693254149363 48-49 19.423656255748863 31.295938743778706 31.391776791667347 17.88862820880508 50-51 23.799943510256625 26.73190141404645 31.00039930102289 18.467755774674032 52-53 19.012547221331328 26.681323150594206 26.90521073732143 27.40091889075304 54-55 23.784667404145186 26.72279852226002 30.958685896766923 18.53384817682787 56-57 19.402582829891898 26.710565424009562 30.89121657499524 22.995635171103295 58-59 19.256775653380142 26.579221502166984 31.578853339535605 22.58514950491727 60-61 23.19998009570094 26.87968719525717 31.535105706522465 18.38522700251943 62-63 18.960637697776008 26.929422572603496 31.189666809289147 22.92027292033135 64-65 23.39425471518407 26.777477436417037 31.009051811138537 18.819216037260357 66-67 19.542507517395872 35.02724095875351 26.46851376641905 18.961737757431568 68-69 19.565076187224122 35.423423303079964 26.442005288147254 18.569495221548657 70-71 18.91987748833043 36.29944224528583 26.339508009646238 18.441172256737488 72-73 18.818809675684882 36.0352468515284 26.273131372503183 18.872812100283536 74-75 19.57366891356146 34.70387013210833 26.210614490434054 19.511846463896156 76-77 19.92660644203031 33.575023282978435 26.623806173420373 19.874564101570886 78-79 19.740645456412242 33.09602712579739 27.23449757674207 19.928829841048305 80-81 19.146196658995564 33.207358918227264 27.202175913620597 20.444268509156576 82-83 18.592568775421213 32.60070489923667 27.432156119555078 21.374570205787037 84-85 18.766261898262808 31.924849474402095 27.876263910001008 21.432624717334086 86-87 18.925787981692803 31.851256014897977 28.42849184699115 20.794464156418073 88-89 19.255915431912847 32.224269849612355 27.917946943930033 20.60186777454476 90-91 19.708078068471202 31.7123778051307 27.50001502703865 21.079529099359448 92-93 19.166943911110753 32.117365923918776 27.526799182830352 21.188890982140112 94-95 18.928374037691125 32.47404849781185 27.527959174435818 21.069618290061204 96-97 19.3881615236744 32.91055804300932 26.840636460845108 20.860643972471177 98-99 19.902086584088575 32.05142249487004 26.542678185918263 21.50381273512312 100-101 19.78180068125292 32.026051127701024 26.23727159507967 21.954876595966386 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 835.0 1 560.5 2 281.5 3 418.5 4 492.0 5 377.5 6 350.5 7 387.0 8 414.0 9 433.0 10 439.0 11 423.5 12 432.5 13 449.5 14 431.5 15 455.5 16 492.5 17 493.0 18 547.5 19 619.5 20 668.5 21 763.0 22 922.0 23 1116.5 24 1439.0 25 2093.0 26 3294.5 27 5342.5 28 7473.0 29 9417.0 30 11773.5 31 14103.5 32 17510.5 33 26417.0 34 43110.0 35 73175.5 36 112245.5 37 154284.0 38 201255.5 39 233132.5 40 243735.5 41 230167.0 42 207803.5 43 202353.0 44 192618.5 45 174933.5 46 165842.0 47 147223.0 48 114029.5 49 88363.5 50 69761.5 51 56736.5 52 47173.0 53 36437.0 54 27212.0 55 19503.5 56 13266.5 57 8694.5 58 5983.5 59 4331.5 60 3065.5 61 2204.5 62 1567.5 63 1083.0 64 769.0 65 527.5 66 356.5 67 281.5 68 218.0 69 148.0 70 103.0 71 86.0 72 65.0 73 41.0 74 28.0 75 20.5 76 15.5 77 14.0 78 10.5 79 5.0 80 4.0 81 4.0 82 3.5 83 3.5 84 2.5 85 2.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16249044738457818 2 0.0 3 0.0015023772616203038 4 0.010817116283666188 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.502377261620304E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 5.842578239634515E-4 38-39 0.0017360803912056845 40-41 0.0054920235452564446 42-43 0.002337031295853806 44-45 0.0010850502445035528 46-47 0.001135129486557563 48-49 0.0032050714914566483 50-51 6.343370660174617E-4 52-53 0.014906921051410348 54-55 0.027226414596696842 56-57 0.028812257261740498 58-59 0.021784470293494406 60-61 0.030882199266639578 62-63 0.005558795867995124 64-65 0.010933967848458877 66-67 0.028879029584479174 68-69 0.027393345403543542 70-71 0.03091558542800892 72-73 0.0314831501712877 74-75 0.039896462836361404 76-77 0.01923042894873989 78-79 0.010616799315450147 80-81 0.015524565036743139 82-83 0.0071780246944081185 84-85 0.0110341263325669 86-87 0.03408727075809623 88-89 0.0153242480685271 90-91 0.021734391051440396 92-93 0.05024617286085683 94-95 0.057975069217859054 96-97 0.04098151308086496 98-99 0.03595689579477927 100-101 0.035522875696977846 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2995253.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.096544247013306 #Duplication Level Percentage of deduplicated Percentage of total 1 85.70721516340758 31.794414995976396 2 6.489894338029595 4.8150530493831205 3 2.1362860352941913 2.3774648829770277 4 1.0832070613845695 1.6073295472531977 5 0.6986897284603147 1.295948721338089 6 0.5213910328515027 1.1605083312103046 7 0.38697852450934134 1.0048896169973291 8 0.3491688877700394 1.0362367275873352 9 0.28129912485514763 0.9391702888651595 >10 1.8850559877477215 13.897493316920636 >50 0.2425172598232312 6.210427500328184 >100 0.1847908004851499 14.964556536873234 >500 0.026686864102718887 6.790773088804242 >1k 0.006454373680491524 3.9109453689379055 >5k 2.7361319942765773E-4 0.5664096454056068 >10k+ 9.120439900797362E-5 7.628378381142399 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 225467 7.527477645461002 TruSeq Adapter, Index 6 (97% over 36bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6171 0.2060260018101977 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 5485 0.1831230951108304 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5085 0.16976863056309435 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 4862 0.1623235165777315 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 4838 0.16152224870486734 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4176 0.1394206098783642 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3275 0.10933967848458878 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005074696528139693 0.0 0.0 2 0.005608875110049134 0.0 0.0 3 0.006209826014697256 0.0 0.0 4 0.006610459951129337 0.0 0.0 5 0.007378341662624159 0.0 3.3386161369340086E-5 6 0.008346540342335021 0.0 3.3386161369340086E-5 7 0.010182779217648727 0.0 3.3386161369340086E-5 8 0.012386265868025171 0.0 3.3386161369340086E-5 9 0.014155732420600196 0.0 3.3386161369340086E-5 10-11 0.022735975892520598 0.0 3.3386161369340086E-5 12-13 0.030398099926784147 0.0 3.3386161369340086E-5 14-15 0.04028040369210881 0.0 3.3386161369340086E-5 16-17 0.051214371540567696 0.0 3.3386161369340086E-5 18-19 0.06435182603940301 0.0 3.3386161369340086E-5 20-21 0.07415066440130433 0.0 3.3386161369340086E-5 22-23 0.08418320589279102 0.0 3.3386161369340086E-5 24-25 0.09650269943807752 0.0 3.3386161369340086E-5 26-27 0.10800423202981518 0.0 3.3386161369340086E-5 28-29 0.12174263743329862 0.0 3.3386161369340086E-5 30-31 0.21567460244593695 0.0 3.3386161369340086E-5 32-33 0.23407037736044334 0.0 3.3386161369340086E-5 34-35 0.24725791110133266 0.0 3.3386161369340086E-5 36-37 0.2605122171649607 0.0 3.3386161369340086E-5 38-39 0.2762370991699199 0.0 3.3386161369340086E-5 40-41 0.2922791497078878 0.0 3.3386161369340086E-5 42-43 0.309823577507476 0.0 3.3386161369340086E-5 44-45 0.32695067828994745 0.0 3.3386161369340086E-5 46-47 0.3468154443047048 0.0 3.3386161369340086E-5 48-49 0.3666969034001468 0.0 3.3386161369340086E-5 50-51 0.388748462984596 0.0 3.3386161369340086E-5 52-53 0.4119351520556026 0.0 3.3386161369340086E-5 54-55 0.4371917831315084 0.0 3.3386161369340086E-5 56-57 0.4659372680705103 0.0 3.3386161369340086E-5 58-59 0.49755396288727527 0.0 3.3386161369340086E-5 60-61 0.5314242235964708 0.0 3.3386161369340086E-5 62-63 0.5675814363594661 0.0 3.3386161369340086E-5 64-65 0.6076949092447282 0.0 3.3386161369340086E-5 66-67 0.6506795920077536 0.0 3.3386161369340086E-5 68-69 0.6960346922280021 0.0 3.3386161369340086E-5 70-71 0.7475829253822632 0.0 3.3386161369340086E-5 72-73 0.8004165257492439 0.0 3.3386161369340086E-5 74-75 0.857573634013554 0.0 3.3386161369340086E-5 76-77 0.919705280321896 0.0 3.3386161369340086E-5 78-79 0.9911182794909144 0.0 3.3386161369340086E-5 80-81 1.0685407877064141 0.0 3.3386161369340086E-5 82-83 1.1529410036481058 0.0 3.3386161369340086E-5 84-85 1.2453038190763852 0.0 3.3386161369340086E-5 86-87 1.3443104806171633 0.0 3.3386161369340086E-5 88-89 1.4512296624024748 0.0 3.3386161369340086E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 31970 0.0 78.451385 3 GATCGGA 32055 0.0 78.35805 1 CGGAAGA 31910 0.0 78.15109 4 GAAGAGC 32055 0.0 77.68478 6 ATCGGAA 32360 0.0 77.52057 2 GGAAGAG 32960 0.0 75.8399 5 CGACGTA 20 0.0020767646 71.22957 6 GAGCACA 40270 0.0 61.648567 9 AGAGCAC 40275 0.0 61.593754 8 AAGAGCA 40800 0.0 60.812828 7 TCGGACG 140 0.0 54.277397 3 CGCGAGG 55 8.110783E-7 51.896023 1 ATCGGAC 150 0.0 50.658905 2 CATCGGA 40 5.3498405E-4 47.571346 1 CGCACCG 40 5.3936493E-4 47.49272 3 ACGCGGC 50 2.8306544E-5 47.49272 2 GCCGTCT 27150 0.0 44.829433 50-51 ATGCCGT 27130 0.0 44.787952 48-49 CGTCTTC 27205 0.0 44.67955 52-53 TCGTATG 27740 0.0 44.62203 44-45 >>END_MODULE