FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_41442-1.34100000035721.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_41442-1.34100000035721.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995253
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2254677.527477645461002TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61710.2060260018101977No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT54850.1831230951108304TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC50850.16976863056309435No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC48620.1623235165777315No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG48380.16152224870486734No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG41760.1394206098783642No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA32750.10933967848458878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG319700.078.4513853
GATCGGA320550.078.358051
CGGAAGA319100.078.151094
GAAGAGC320550.077.684786
ATCGGAA323600.077.520572
GGAAGAG329600.075.83995
CGACGTA200.002076764671.229576
GAGCACA402700.061.6485679
AGAGCAC402750.061.5937548
AAGAGCA408000.060.8128287
TCGGACG1400.054.2773973
CGCGAGG558.110783E-751.8960231
ATCGGAC1500.050.6589052
CATCGGA405.3498405E-447.5713461
CGCACCG405.3936493E-447.492723
ACGCGGC502.8306544E-547.492722
GCCGTCT271500.044.82943350-51
ATGCCGT271300.044.78795248-49
CGTCTTC272050.044.6795552-53
TCGTATG277400.044.6220344-45
Produced by FastQC (version 0.11.2)