FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_40825-1.341000000356f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_40825-1.341000000356f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808189
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT670313.707079293149112TruSeq Adapter, Index 27 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124580.6889766501178803No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA41200.22785228756507203No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC37710.20855120786599188No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36570.20224655719064769No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT22130.12238764863628747TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA105550.070.606094
GATCGGA107200.069.937811
ATCGGAA107250.069.710292
TCGGAAG107700.069.507233
GAAGAGC110350.067.521796
GGAAGAG130300.057.2929885
GAGCACA143050.051.8877039
AAGAGCA147100.050.3622677
AGAGCAC149750.049.7247438
TATGCCG86150.042.78779648-49
GTATGCC87150.042.58836746-47
GATCTCG84250.042.4442140-41
CTCGTAT84450.042.26050644-45
TCGTATG88800.041.9551644-45
GCCGTCT88150.041.90246650-51
TACTCGA87200.041.82520736-37
CTCGATC84450.041.8094738-39
CTGCTTG89400.041.74286358-59
TCTCGTA85750.041.6746342-43
TCGATCT85600.041.55290638-39
Produced by FastQC (version 0.11.2)