FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_40255-1.341000000357f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_40255-1.341000000357f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7531203
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1920692.550309691559237TruSeq Adapter, Index 7 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148810.1975912745945103No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA119230.1583146809347723No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC101680.1350116309439541No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG93980.1247875007485524No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC93920.12470783220157523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA336050.064.1607364
TCGGAAG338500.064.090043
GATCGGA346250.062.8046841
GAAGAGC346700.062.1574866
ATCGGAA352400.061.4677472
GGAAGAG391950.055.199585
GCCGTCT246300.043.33076550-51
ATGCCGT245750.043.16132448-49
TGCCGTC246450.043.07307450-51
TATGCCG247600.043.001948-49
TCGTATG250400.042.85325644-45
CGTATGC249950.042.6300946-47
CATCTCG246350.042.49989740-41
CTCGTAT248500.042.43558544-45
TCTCGTA248950.042.41596642-43
ATCTCGT247150.042.24450742-43
CGTCTTC254400.042.09216352-53
GTATGCC257550.041.60263446-47
GTCACGA258300.040.77743528-29
TCACGAG258600.040.49167330-31