##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_40255-1.341000000357f8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7531203 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67675721926497 33.0 33.0 33.0 33.0 33.0 2 32.65980667364828 33.0 33.0 33.0 33.0 33.0 3 32.724669485074294 33.0 33.0 33.0 33.0 33.0 4 36.67687937770367 37.0 37.0 37.0 37.0 37.0 5 36.68212448927482 37.0 37.0 37.0 37.0 37.0 6 36.5705862662313 37.0 37.0 37.0 37.0 37.0 7 36.50073235311809 37.0 37.0 37.0 37.0 37.0 8 36.444910062841224 37.0 37.0 37.0 37.0 37.0 9 36.465070586996525 37.0 37.0 37.0 37.0 37.0 10-11 36.33338458676522 37.0 37.0 37.0 37.0 37.0 12-13 36.37429080055338 37.0 37.0 37.0 37.0 37.0 14-15 38.298198972992765 40.0 37.0 40.0 37.0 40.0 16-17 38.25172040907674 40.0 37.0 40.0 37.0 40.0 18-19 38.16111722124606 40.0 37.0 40.0 37.0 40.0 20-21 38.15079974606978 40.0 37.0 40.0 37.0 40.0 22-23 38.04097678684269 40.0 37.0 40.0 37.0 40.0 24-25 38.055272909255 40.0 37.0 40.0 37.0 40.0 26-27 37.97545405428588 40.0 37.0 40.0 35.0 40.0 28-29 37.82490094875945 40.0 37.0 40.0 33.0 40.0 30-31 37.72102790218243 40.0 37.0 40.0 33.0 40.0 32-33 37.61152587441873 40.0 37.0 40.0 33.0 40.0 34-35 37.50055588728653 40.0 37.0 40.0 33.0 40.0 36-37 37.37595693543249 40.0 37.0 40.0 33.0 40.0 38-39 37.31281257456478 40.0 37.0 40.0 33.0 40.0 40-41 37.19480911615315 40.0 37.0 40.0 33.0 40.0 42-43 37.0795669031893 40.0 37.0 40.0 33.0 40.0 44-45 36.97683012926355 37.0 37.0 40.0 33.0 40.0 46-47 36.931650096272804 37.0 37.0 40.0 33.0 40.0 48-49 36.73623098461162 37.0 37.0 40.0 33.0 40.0 50-51 36.60128036649657 37.0 37.0 40.0 33.0 40.0 52-53 36.43595618123692 37.0 37.0 40.0 33.0 40.0 54-55 36.07181548552071 37.0 37.0 40.0 33.0 40.0 56-57 35.939961251874365 37.0 37.0 40.0 33.0 40.0 58-59 35.801238195279026 37.0 37.0 40.0 30.0 40.0 60-61 35.48287664002683 37.0 33.0 40.0 27.0 40.0 62-63 35.25080681001428 37.0 33.0 40.0 27.0 40.0 64-65 35.01923451007761 37.0 33.0 37.0 27.0 40.0 66-67 34.493920094837435 37.0 33.0 37.0 27.0 40.0 68-69 34.26646533097036 37.0 33.0 37.0 27.0 40.0 70-71 33.97417809611559 37.0 33.0 37.0 27.0 40.0 72-73 33.503685599764076 37.0 33.0 37.0 27.0 37.0 74-75 32.982399558211355 37.0 33.0 37.0 24.5 37.0 76-77 30.319867290790064 33.0 30.0 35.0 22.0 37.0 78-79 32.67439026673428 35.0 33.0 37.0 27.0 37.0 80-81 33.05788709984314 37.0 33.0 37.0 27.0 37.0 82-83 32.937752175847606 37.0 33.0 37.0 27.0 37.0 84-85 32.82034430621509 37.0 33.0 37.0 27.0 37.0 86-87 32.69071249307713 37.0 33.0 37.0 27.0 37.0 88-89 32.53169287828253 37.0 33.0 37.0 22.0 37.0 90-91 32.439337181589714 33.0 33.0 37.0 22.0 37.0 92-93 32.26853677958222 33.0 33.0 37.0 22.0 37.0 94-95 32.149366176957386 33.0 33.0 37.0 22.0 37.0 96-97 31.985046147341933 33.0 33.0 37.0 22.0 37.0 98-99 31.853980698701125 33.0 33.0 37.0 22.0 37.0 100-101 30.231148994390402 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.12757678476722134 2306 2 0.19269987640365116 2306 3 0.15489350902293353 2306 4 0.16652511259615466 2306 5 0.1549182248938763 2306 6 0.1847459175370858 2306 7 0.22436022854637372 2306 8 0.1944556028288318 2306 9 0.17121751390997275 2306 10-11 0.2719541151906313 2306 12-13 0.24478667045905667 2306 14-15 0.37218662237689415 2306 16-17 0.45733518280644603 2306 18-19 0.4647221337118097 2306 20-21 0.48431315554521603 2306 22-23 0.43439021370653563 2306 24-25 0.39156532063895355 2306 26-27 0.4890791819054954 2306 28-29 0.5100597490470165 2306 30-31 0.582678716593307 2306 32-33 0.5702074401627115 2306 34-35 0.5824819694989074 2306 36-37 0.6041363225365117 2306 38-39 0.5949678819969861 2306 40-41 0.7562805255153435 2306 42-43 0.7160316874939738 2306 44-45 0.6286373256879898 2306 46-47 0.5343957815240259 2306 48-49 0.5995037156854792 2306 50-51 0.6188134923362156 2306 52-53 0.5560255122240036 2306 54-55 0.6782181670421679 2306 56-57 0.7362400461747001 2306 58-59 0.5886186159820781 2306 60-61 0.596691316910956 2306 62-63 0.5312408134156641 2306 64-65 0.5670895921683226 2306 66-67 0.6736260954756617 2306 68-69 0.651730173918132 2306 70-71 0.5556220445183797 2306 72-73 0.46993677391063926 2306 74-75 0.5308901872455181 2306 76-77 0.1821627837330304 2306 78-79 0.5561412556419398 2306 80-81 0.6438719027872892 2306 82-83 0.7585678834652754 2306 84-85 0.7570678783926965 2306 86-87 0.9001516122210589 2306 88-89 0.7367899495887826 2306 90-91 0.6880623688174623 2306 92-93 0.5565356643617108 2306 94-95 0.6059033617611433 2306 96-97 0.7859158785463407 2306 98-99 0.7223874536782802 2306 100-101 0.4111688627726764 2307 1 0.1291128912837891 2307 2 0.20866302515648982 2307 3 0.14783347895303933 2307 4 0.1520908941550303 2307 5 0.11129702581064294 2307 6 0.15239446976168125 2307 7 0.14357521871338008 2307 8 0.16235037861548562 2307 9 0.14802992026050532 2307 10-11 0.23633147416863665 2307 12-13 0.1896238615074921 2307 14-15 0.31692294787933406 2307 16-17 0.3675187198270464 2307 18-19 0.40938043792038314 2307 20-21 0.3711969109138167 2307 22-23 0.42304495277654297 2307 24-25 0.41216780609358494 2307 26-27 0.3726171513648495 2307 28-29 0.3487221586826479 2307 30-31 0.4886945530421656 2307 32-33 0.472253360219149 2307 34-35 0.38755850452102436 2307 36-37 0.3969364441811578 2307 38-39 0.5071106425458467 2307 40-41 0.5864296837305716 2307 42-43 0.5687671132598027 2307 44-45 0.48379412257460785 2307 46-47 0.47141680971790123 2307 48-49 0.5563242120109422 2307 50-51 0.5265187668786737 2307 52-53 0.5099602173712867 2307 54-55 0.6173222082980132 2307 56-57 0.6619238667351297 2307 58-59 0.5356103429341843 2307 60-61 0.3581366858986499 2307 62-63 0.3902718040955264 2307 64-65 0.3754841252397867 2307 66-67 0.47043827378234226 2307 68-69 0.3284187428935823 2307 70-71 0.35564621238161465 2307 72-73 0.3609898644309695 2307 74-75 0.461719984570351 2307 76-77 0.1505465381448019 2307 78-79 0.5140559238684475 2307 80-81 0.49169501699228135 2307 82-83 0.4565783922996616 2307 84-85 0.5167477813831667 2307 86-87 0.5802808524810317 2307 88-89 0.5950276905577709 2307 90-91 0.5291555185079844 2307 92-93 0.4978714654590064 2307 94-95 0.5502678402488002 2307 96-97 0.7009421578085195 2307 98-99 0.5986697617498749 2307 100-101 0.4149684240354574 2201 1 0.09606874593016812 2201 2 0.17219910972423236 2201 3 0.10693725945597521 2201 4 0.10854065103405475 2201 5 0.10325231637123267 2201 6 0.08575675949870032 2201 7 0.13576545100390547 2201 8 0.09688427453037463 2201 9 0.12259218969043673 2201 10-11 0.18742763387260908 2201 12-13 0.14748552510928903 2201 14-15 0.1928897909168228 2201 16-17 0.2593301685121645 2201 18-19 0.34912634547800536 2201 20-21 0.3085343131859517 2201 22-23 0.3188364497473941 2201 24-25 0.3000749350097536 2201 26-27 0.25491642143784077 2201 28-29 0.35141848874052783 2201 30-31 0.46934542342653884 2201 32-33 0.5227198591643045 2201 34-35 0.5042870325955704 2201 36-37 0.40590484409541006 2201 38-39 0.40893884457882024 2201 40-41 0.5448086139982848 2201 42-43 0.4431617081897983 2201 44-45 0.4008297953597264 2201 46-47 0.38222215499481393 2201 48-49 0.35137323106575735 2201 50-51 0.4305161608023411 2201 52-53 0.29669785543129024 2201 54-55 0.6059044068787713 2201 56-57 0.5849683223359463 2201 58-59 0.306141232271834 2201 60-61 0.40904585116449965 2201 62-63 0.4671025445145318 2201 64-65 0.2870827161534919 2201 66-67 0.3923487759315094 2201 68-69 0.3854308933014181 2201 70-71 0.31461038165247857 2201 72-73 0.36233478596663815 2201 74-75 0.29702498575493763 2201 76-77 0.17805784806481384 2201 78-79 0.3316812073215516 2201 80-81 0.3679217625557527 2201 82-83 0.4926090898481448 2201 84-85 0.456669965748965 2201 86-87 0.5514772220249071 2201 88-89 0.5008561069433313 2201 90-91 0.4794635093472337 2201 92-93 0.3541415610151333 2201 94-95 0.469214776698891 2201 96-97 0.5119773680432829 2201 98-99 0.4485525729278095 2201 100-101 0.43956002592627286 1101 1 0.08840218675770473 1101 2 0.16426324655608227 1101 3 0.11708352392893318 1101 4 0.10639676176962354 1101 5 0.10910072473345167 1101 6 0.07771484916127491 1101 7 0.13230915049971514 1101 8 0.11411290850639944 1101 9 0.18665134664644967 1101 10-11 0.14693404941626653 1101 12-13 0.110421651844959 1101 14-15 0.203968231713354 1101 16-17 0.21787805097964963 1101 18-19 0.2986926218580095 1101 20-21 0.2706797373436203 1101 22-23 0.18113706866596146 1101 24-25 0.21379176426140845 1101 26-27 0.22449693046425523 1101 28-29 0.2703706992968762 1101 30-31 0.3639181029470322 1101 32-33 0.2492614191119671 1101 34-35 0.3807161112255102 1101 36-37 0.3112044893645347 1101 38-39 0.33534877580319034 1101 40-41 0.486078559858413 1101 42-43 0.37095002005529665 1101 44-45 0.33704001991925736 1101 46-47 0.3993414084475475 1101 48-49 0.33236435349132165 1101 50-51 0.23235717997145144 1101 52-53 0.2796467419360056 1101 54-55 0.3478406359406563 1101 56-57 0.41563176831079574 1101 58-59 0.32759686920601894 1101 60-61 0.330911413121477 1101 62-63 0.36540540220666884 1101 64-65 0.2505577609599712 1101 66-67 0.44597337205303234 1101 68-69 0.4003363345754778 1101 70-71 0.36019539303239156 1101 72-73 0.11474604123024079 1101 74-75 0.16409121562303142 1101 76-77 -0.04991279051278852 1101 78-79 0.3698709790410746 1101 80-81 0.4638682490455892 1101 82-83 0.46896312925304073 1101 84-85 0.4587965680844803 1101 86-87 0.46677119284023405 1101 88-89 0.33579942442127475 1101 90-91 0.34897432801897565 1101 92-93 0.356291806908132 1101 94-95 0.4007990810976807 1101 96-97 0.49446400361932064 1101 98-99 0.31776222694116285 1101 100-101 0.30389949469817523 2304 1 0.07950982365701975 2304 2 0.17850345345006247 2304 3 0.10556275220385913 2304 4 0.10474955727521262 2304 5 0.07827565706041639 2304 6 0.04759378961998095 2304 7 0.09558763738991871 2304 8 0.08909885798158967 2304 9 0.09601610375897707 2304 10-11 0.11367058451293843 2304 12-13 0.16477602474149933 2304 14-15 0.17875606055601168 2304 16-17 0.19913547415730903 2304 18-19 0.3092430017710299 2304 20-21 0.2739336275826858 2304 22-23 0.22216414830828768 2304 24-25 0.14240480731967864 2304 26-27 0.14982968366459204 2304 28-29 0.27650902232282704 2304 30-31 0.35670879787720366 2304 32-33 0.2762265030089637 2304 34-35 0.2581061823343447 2304 36-37 0.2743574271316831 2304 38-39 0.22141243895064378 2304 40-41 0.370537362182894 2304 42-43 0.36208770641685106 2304 44-45 0.31268686546665947 2304 46-47 0.26825180945830596 2304 48-49 0.23036165974226464 2304 50-51 0.2614272492800538 2304 52-53 0.2549938200854598 2304 54-55 0.4221772288859782 2304 56-57 0.436660875948796 2304 58-59 0.3764498716275355 2304 60-61 0.38993171283673433 2304 62-63 0.2886768857884121 2304 64-65 0.23536128138482582 2304 66-67 0.3594624521607841 2304 68-69 0.30928189482917645 2304 70-71 0.22596998858583817 2304 72-73 0.11822548081187989 2304 74-75 0.1557176242430245 2304 76-77 0.021006602285002174 2304 78-79 0.241386572379362 2304 80-81 0.32429848599451816 2304 82-83 0.3505201688952795 2304 84-85 0.33931911039522333 2304 86-87 0.422895369292867 2304 88-89 0.3720206894514888 2304 90-91 0.19429793191272893 2304 92-93 0.15944979957112793 2304 94-95 0.17312504943131302 2304 96-97 0.31260179042016745 2304 98-99 0.2798710625258707 2304 100-101 0.2328606587182911 2202 1 0.09958358064938011 2202 2 0.12827203112561847 2202 3 0.10561599353616913 2202 4 0.06787106109439378 2202 5 0.0865049923246346 2202 6 0.05283451143046847 2202 7 0.06603692324618038 2202 8 0.07694563652604813 2202 9 0.040426828692957884 2202 10-11 0.17675623916836258 2202 12-13 0.11506281805154828 2202 14-15 0.1271749704574816 2202 16-17 0.10155653500860495 2202 18-19 0.23434309547577215 2202 20-21 0.19634769607232982 2202 22-23 0.17190521796215563 2202 24-25 0.22579138198532434 2202 26-27 0.14909761534531185 2202 28-29 0.23490979467709394 2202 30-31 0.23024909801634408 2202 32-33 0.2659873074500041 2202 34-35 0.20556763560975355 2202 36-37 0.2353315305476471 2202 38-39 0.3222461982146356 2202 40-41 0.37321895062029853 2202 42-43 0.27782087505699593 2202 44-45 0.21533508933581658 2202 46-47 0.17598546791870717 2202 48-49 0.16852540168508057 2202 50-51 0.19161011550943385 2202 52-53 0.27579077184171297 2202 54-55 0.42850496123114823 2202 56-57 0.4388171499074005 2202 58-59 0.3719021744926323 2202 60-61 0.32112084024678467 2202 62-63 0.25032892883579905 2202 64-65 0.28738072511938384 2202 66-67 0.3138648004584823 2202 68-69 0.3504223596615006 2202 70-71 0.2662009139911845 2202 72-73 0.23661911882761189 2202 74-75 0.19510023775355023 2202 76-77 -0.018871155616015045 2202 78-79 0.29685782915876047 2202 80-81 0.4113711355671654 2202 82-83 0.3865077601154283 2202 84-85 0.32414710539763547 2202 86-87 0.32487009030828773 2202 88-89 0.261809356856439 2202 90-91 0.25105762893405625 2202 92-93 0.16766671185780524 2202 94-95 0.27873011942591575 2202 96-97 0.40516805813178536 2202 98-99 0.4157145472065622 2202 100-101 0.43679004651395914 1102 1 0.12904962329644576 1102 2 0.1651703017672972 1102 3 0.07785082275694322 1102 4 0.07821040930720358 1102 5 0.10125303475282266 1102 6 0.06475778148203659 1102 7 0.033618965300981074 1102 8 0.10637439464549203 1102 9 0.06833738230650255 1102 10-11 0.1662646681539499 1102 12-13 0.1291642823946617 1102 14-15 0.15378123618940265 1102 16-17 0.1650924547615702 1102 18-19 0.252418446701391 1102 20-21 0.2333791449007876 1102 22-23 0.16769863807454755 1102 24-25 0.1457048872459339 1102 26-27 0.11430578639706823 1102 28-29 0.17718476243535974 1102 30-31 0.26039269977681556 1102 32-33 0.280426746647791 1102 34-35 0.21906436637361537 1102 36-37 0.2609226646625089 1102 38-39 0.18577061072850398 1102 40-41 0.28454682163621925 1102 42-43 0.2225829085712263 1102 44-45 0.20623310461871824 1102 46-47 0.1880963676614229 1102 48-49 0.2907184668702385 1102 50-51 0.2561210658947246 1102 52-53 0.24124585230119067 1102 54-55 0.3395558806300514 1102 56-57 0.28961332210075597 1102 58-59 0.23957858127273113 1102 60-61 0.2791086881731246 1102 62-63 0.2797427999676074 1102 64-65 0.24724647936123745 1102 66-67 0.2337787080701048 1102 68-69 0.2317910397999512 1102 70-71 0.13232826935404063 1102 72-73 0.08986627930236324 1102 74-75 0.19617773377832037 1102 76-77 0.013218618015937267 1102 78-79 0.3226608681428189 1102 80-81 0.4369107687517868 1102 82-83 0.4025749774128755 1102 84-85 0.3509058621651846 1102 86-87 0.37754843373794955 1102 88-89 0.35595426438008815 1102 90-91 0.33935087737668823 1102 92-93 0.24359289707660992 1102 94-95 0.1606665168500072 1102 96-97 0.3379444571867438 1102 98-99 0.3833321302679096 1102 100-101 0.3117280844572683 2305 1 0.10759057650095372 2305 2 0.142289443120589 2305 3 0.09363560617484268 2305 4 0.08591736273260153 2305 5 0.06392902817088952 2305 6 -0.027428792011249925 2305 7 0.03943877150950925 2305 8 0.05814394715017812 2305 9 0.02968693561133051 2305 10-11 0.09849104824041177 2305 12-13 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14 962.0 15 2729.0 16 6107.0 17 9890.0 18 12088.0 19 14121.0 20 16967.0 21 21162.0 22 26227.0 23 32863.0 24 41318.0 25 51735.0 26 66461.0 27 89719.0 28 129338.0 29 174574.0 30 162468.0 31 167792.0 32 223406.0 33 317333.0 34 493498.0 35 867581.0 36 1661487.0 37 2374457.0 38 566149.0 39 305.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.25807245291651 27.353363239434557 20.302603107556898 15.085961200092033 2 31.60027687475693 25.699068263065012 28.24334970123631 14.457305160941752 3 35.025129164569805 19.972381155981406 27.011123223862683 17.99136645558611 4 30.95587219802401 19.68092000424944 28.281977053011794 21.08123074471476 5 31.775733571382954 18.227911264641254 31.23528870487225 18.761066459103546 6 29.108350950040784 18.795151319118606 33.2541560757292 18.842341655111408 7 31.927648212377225 21.917481177973823 25.83131805104709 20.32355255860186 8 29.899897798532322 28.297842987368682 22.14988760759735 19.652371606501646 9 29.42397117698195 25.530303724385067 24.719636424619015 20.326088674013963 10-11 24.542812084603217 28.717350999568065 28.850716147207823 17.8891207686209 12-13 22.717539282900752 27.926096269082112 28.558491916895612 20.797872531121524 14-15 22.774382791168954 28.504124772629286 28.436101111601957 20.285391324599804 16-17 23.698544575149548 27.52525061401213 28.90938539301092 19.866819417827404 18-19 21.425620847028025 27.383639771760237 30.687135906441508 20.503603474770234 20-21 22.71865041427661 27.79260277246654 30.418744688761418 19.07000212449543 22-23 21.341255498798994 30.545119695559404 28.722154135003898 19.39147067063771 24-25 21.39968873498696 26.928964735116022 30.63175298820122 21.039593541695794 26-27 21.15500671008337 27.14456110132737 29.133997583121847 22.566434605467414 28-29 22.360179376389137 29.310084989078106 28.507736413425583 19.821999221107173 30-31 20.249354585183802 28.794662685363814 29.87590428780103 21.080078441651352 32-33 20.076931932388494 30.16709282700254 28.68523793609069 21.070737304518282 34-35 22.082235733122584 29.8996189586179 28.784458472305154 19.233686835954362 36-37 21.674373551432087 30.504285868881826 29.031588158241544 18.789752421444547 38-39 20.420091532773615 28.682320567069258 31.26106333055064 19.636524569606483 40-41 20.619970349682927 28.12200914925208 28.081767631093168 23.17625286997183 42-43 20.86469279101115 29.657484047814762 29.76846500836573 19.709358152808353 44-45 20.307966309375665 28.148417844048613 29.975119225357837 21.568496621217893 46-47 22.008398096350053 28.045282558889216 28.62694304818087 21.31937629657986 48-49 20.8971329517578 29.672909931836788 29.382834359219533 20.04712275718588 50-51 22.081760337786598 28.34599331535953 28.831835741322486 20.74041060553139 52-53 20.57871250329097 28.161031543168047 27.81571637210905 23.44453958143193 54-55 22.439032002927288 28.041041790214173 29.328244670218638 20.1916815366399 56-57 20.9738471418202 27.607884441569635 29.420056169440716 21.998212247169448 58-59 20.71091614671735 27.810521968024776 29.710189678574366 21.768372206683505 60-61 21.826579577645877 27.97257296202553 29.510703363914377 20.69014409641422 62-63 20.191952482946633 28.105326546953368 29.570389216507742 22.132331753592265 64-65 22.030114866911532 28.21701784255668 29.518501555103487 20.2343657354283 66-67 21.17649902723012 30.691427671291972 27.80445014328566 20.32762315819225 68-69 20.980765348407253 30.416084482648703 28.044751577873644 20.558398591070404 70-71 20.725989126048738 31.14079793136187 27.911107693133236 20.222105249456156 72-73 20.83090017229204 31.352821600134362 27.699078729354326 20.117199498219268 74-75 21.631996835368632 30.58625380518894 27.430435959053728 20.351313400388698 76-77 21.707987213616004 29.618232583020752 27.698143432395998 20.97563677096724 78-79 21.339417395071116 29.56146603905829 28.297367492184332 20.801749073686263 80-81 20.91695652912985 30.117504523235567 28.10341635759218 20.8621225900424 82-83 20.188459563962343 30.0595474431502 28.346703610877917 21.405289382009535 84-85 20.436657423866077 29.83435531781293 28.46082305035526 21.268164207965736 86-87 20.270983138733946 30.03953663811925 28.916550271422132 20.77292995172467 88-89 20.3028456076776 30.41464694229761 28.7818665808943 20.500640869130493 90-91 20.81716428961415 29.56927247969105 28.541875537796813 21.07168769289798 92-93 20.460961201222215 29.961521582327865 28.037979312162996 21.53953790428692 94-95 20.26577415427857 30.356731713661784 27.683060674111324 21.69443345794832 96-97 20.534212473928548 30.72183660452867 27.385945966912413 21.358004954630374 98-99 20.701289624071176 29.984154821303072 27.696081263756938 21.61847429086881 100-101 20.95133754861875 30.123169715403147 27.274737217661905 21.650755518316195 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2946.0 1 1870.5 2 918.5 3 1467.0 4 1547.0 5 1079.0 6 1058.5 7 1203.0 8 1257.5 9 1211.5 10 1154.0 11 1138.0 12 1145.0 13 1187.5 14 1221.5 15 1262.0 16 1384.5 17 1567.5 18 1760.0 19 2088.5 20 2660.0 21 3435.5 22 4386.0 23 5928.5 24 8287.5 25 11125.5 26 14879.5 27 19894.5 28 25873.5 29 32847.0 30 40857.0 31 50674.5 32 65960.5 33 88580.5 34 119855.0 35 170221.5 36 239993.5 37 325136.0 38 408083.5 39 448813.0 40 474071.5 41 469817.5 42 443969.5 43 454702.5 44 452391.0 45 438830.0 46 449433.0 47 418011.5 48 332645.0 49 261807.5 50 218223.0 51 192506.5 52 173231.0 53 148207.5 54 116427.5 55 85446.5 56 64046.0 57 52756.5 58 45308.5 59 35458.0 60 25548.5 61 18845.0 62 13484.0 63 9269.0 64 6620.5 65 4629.5 66 3296.5 67 2483.5 68 1764.0 69 1148.5 70 709.0 71 460.5 72 330.0 73 245.0 74 174.5 75 120.0 76 76.5 77 56.0 78 45.5 79 33.0 80 24.5 81 17.5 82 13.5 83 8.0 84 5.0 85 7.0 86 5.5 87 2.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16083751825571557 2 0.0 3 0.001500424301403109 4 0.010662307203776077 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.9834273488578116E-5 22-23 1.7261518511717185E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.701292874458436E-4 38-39 0.0017261518511717184 40-41 0.005623271607470944 42-43 0.0024099735460589764 44-45 0.0011551939311687655 46-47 0.0012149453414016325 48-49 0.003113712377690523 50-51 7.634902418644139E-4 52-53 0.014944491603798224 54-55 0.027193530701535996 56-57 0.029185244375964904 58-59 0.021550341957320765 60-61 0.03126990468853382 62-63 0.0057494134735181085 64-65 0.011100484212150437 66-67 0.02903918537317345 68-69 0.027658263892236076 70-71 0.03166824742341961 72-73 0.03208650729504967 74-75 0.039781161123926684 76-77 0.019578545419636145 78-79 0.010848200480056108 80-81 0.01586731893961695 82-83 0.007555233871666983 84-85 0.011120401348894726 86-87 0.03474212552762155 88-89 0.015860679894035522 90-91 0.021776069507089373 92-93 0.04961358763002405 94-95 0.05690989872401528 96-97 0.04107577501230547 98-99 0.03624918887460609 100-101 0.03623591078344323 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7531203.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.57801622527256 #Duplication Level Percentage of deduplicated Percentage of total 1 88.16048231962242 35.77377481993493 2 5.881882859323364 4.773502762015521 3 1.6840966725684254 2.0501190631322728 4 0.8535974683087018 1.385491676755283 5 0.5985497823956581 1.2143981390842187 6 0.3798458818532552 0.9248035414166612 7 0.26138436134181886 0.7424521198892582 8 0.22885263947029544 0.742910889409768 9 0.1858502198511975 0.6787289912933129 >10 1.3741094332065198 11.396472154815127 >50 0.18023175912592104 5.166622527211709 >100 0.17223700207896278 14.738826070984542 >500 0.02378518614657382 6.805576256005455 >1k 0.014734760461293545 9.972240179119845 >5k 2.2896193559640058E-4 0.5974377375836751 >10k+ 1.3069230998221618E-4 3.036643071348601 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 192069 2.550309691559237 TruSeq Adapter, Index 7 (97% over 38bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14881 0.1975912745945103 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 11923 0.1583146809347723 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10168 0.1350116309439541 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 9398 0.1247875007485524 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 9392 0.12470783220157523 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001779264215823156 0.0 0.0 2 0.0020581041302432028 0.0 0.0 3 0.0024431687739661246 0.0 0.0 4 0.0027883991442004684 0.0 0.0 5 0.003279688517226265 0.0 0.0 6 0.003903758801880656 0.0 0.0 7 0.0044348824483950305 0.0 0.0 8 0.005125343188863718 0.0 0.0 9 0.00592202865863528 0.0 0.0 10-11 0.010097988329354553 0.0 0.0 12-13 0.013975190948909491 0.0 0.0 14-15 0.018881445633586028 0.0 6.639045581429687E-6 16-17 0.025082314206641358 0.0 1.3278091162859373E-5 18-19 0.032591074759238337 0.0 1.3278091162859373E-5 20-21 0.037742974130427764 0.0 1.3278091162859373E-5 22-23 0.04270234117975574 0.0 1.3278091162859373E-5 24-25 0.048783706932345336 0.0 1.3278091162859373E-5 26-27 0.055635201972380775 0.0 1.3278091162859373E-5 28-29 0.06340952434823494 0.0 1.3278091162859373E-5 30-31 0.11243887596709318 0.0 2.6556182325718747E-5 32-33 0.12404392764343225 0.0 4.64733190700078E-5 34-35 0.13336514763975954 0.0 5.311236465143749E-5 36-37 0.1430581541886469 0.0 5.311236465143749E-5 38-39 0.15382668612172584 0.0 5.975141023286718E-5 40-41 0.16549812825387922 0.0 6.639045581429686E-5 42-43 0.17788658730882703 0.0 6.639045581429686E-5 44-45 0.1919281687135508 0.0 7.966854697715623E-5 46-47 0.20642120521781182 0.0 7.966854697715623E-5 48-49 0.22233499747649876 0.0 7.966854697715623E-5 50-51 0.2387998305184444 0.0 7.966854697715623E-5 52-53 0.2571634305966789 0.0 7.966854697715623E-5 54-55 0.2771137625688751 0.0 7.966854697715623E-5 56-57 0.29842509888526436 0.0 7.966854697715623E-5 58-59 0.32263105907515705 0.0 7.966854697715623E-5 60-61 0.3487357863013386 0.0 9.294663814001562E-5 62-63 0.37686542242985616 0.0 1.0622472930287499E-4 64-65 0.40705980173419837 0.0 1.0622472930287499E-4 66-67 0.4390400842999452 0.0 1.1286377488430466E-4 68-69 0.4753357464936213 0.0 1.1950282046573436E-4 70-71 0.5139484355952163 0.0 1.1950282046573436E-4 72-73 0.5577196631135823 0.0 1.1950282046573436E-4 74-75 0.6062710034505776 0.0 1.2614186604716405E-4 76-77 0.6602730002099266 0.0 1.3278091162859373E-4 78-79 0.7216244204279183 0.0 1.3278091162859373E-4 80-81 0.788167574290588 0.0 1.3278091162859373E-4 82-83 0.8635937711412107 0.0 1.3278091162859373E-4 84-85 0.9474316387435049 0.0 1.460590027914531E-4 86-87 1.040059602695612 0.0 1.460590027914531E-4 88-89 1.1439009146347536 0.0 1.460590027914531E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 33605 0.0 64.160736 4 TCGGAAG 33850 0.0 64.09004 3 GATCGGA 34625 0.0 62.804684 1 GAAGAGC 34670 0.0 62.157486 6 ATCGGAA 35240 0.0 61.467747 2 GGAAGAG 39195 0.0 55.19958 5 GCCGTCT 24630 0.0 43.330765 50-51 ATGCCGT 24575 0.0 43.161324 48-49 TGCCGTC 24645 0.0 43.073074 50-51 TATGCCG 24760 0.0 43.0019 48-49 TCGTATG 25040 0.0 42.853256 44-45 CGTATGC 24995 0.0 42.63009 46-47 CATCTCG 24635 0.0 42.499897 40-41 CTCGTAT 24850 0.0 42.435585 44-45 TCTCGTA 24895 0.0 42.415966 42-43 ATCTCGT 24715 0.0 42.244507 42-43 CGTCTTC 25440 0.0 42.092163 52-53 GTATGCC 25755 0.0 41.602634 46-47 GTCACGA 25830 0.0 40.777435 28-29 TCACGAG 25860 0.0 40.491673 30-31 >>END_MODULE