##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_35011-1.341000000358a7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5918099 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68992255790246 33.0 33.0 33.0 33.0 33.0 2 32.6708507241937 33.0 33.0 33.0 33.0 33.0 3 32.74190985990603 33.0 33.0 33.0 33.0 33.0 4 36.69387500952586 37.0 37.0 37.0 37.0 37.0 5 36.70254603716497 37.0 37.0 37.0 37.0 37.0 6 36.58728875606846 37.0 37.0 37.0 37.0 37.0 7 36.52047878888136 37.0 37.0 37.0 37.0 37.0 8 36.46954216210307 37.0 37.0 37.0 37.0 37.0 9 36.496359050431565 37.0 37.0 37.0 37.0 37.0 10-11 36.364898086361855 37.0 37.0 37.0 37.0 37.0 12-13 36.401191581959004 37.0 37.0 37.0 37.0 37.0 14-15 38.329796781027156 40.0 37.0 40.0 37.0 40.0 16-17 38.31173329476239 40.0 37.0 40.0 37.0 40.0 18-19 38.22286092206298 40.0 37.0 40.0 37.0 40.0 20-21 38.2426810534937 40.0 37.0 40.0 37.0 40.0 22-23 38.13658211868372 40.0 37.0 40.0 37.0 40.0 24-25 38.153489997379225 40.0 37.0 40.0 37.0 40.0 26-27 38.1027640801548 40.0 37.0 40.0 37.0 40.0 28-29 37.96096626636357 40.0 37.0 40.0 33.0 40.0 30-31 37.88165203049155 40.0 37.0 40.0 35.0 40.0 32-33 37.78493676770193 40.0 37.0 40.0 33.0 40.0 34-35 37.664782897345916 40.0 37.0 40.0 33.0 40.0 36-37 37.485187642180364 40.0 37.0 40.0 33.0 40.0 38-39 37.52789096633902 40.0 37.0 40.0 33.0 40.0 40-41 37.31113791776717 40.0 37.0 40.0 33.0 40.0 42-43 37.25154006379414 40.0 37.0 40.0 33.0 40.0 44-45 37.21639178391575 40.0 37.0 40.0 33.0 40.0 46-47 37.17120844041305 40.0 37.0 40.0 33.0 40.0 48-49 36.97983448739198 38.5 37.0 40.0 33.0 40.0 50-51 36.88024744094345 37.0 37.0 40.0 33.0 40.0 52-53 36.739190152107966 37.0 37.0 40.0 33.0 40.0 54-55 36.36696395244486 37.0 37.0 40.0 33.0 40.0 56-57 36.27716273418204 37.0 37.0 40.0 33.0 40.0 58-59 36.1213441174269 37.0 37.0 40.0 33.0 40.0 60-61 35.85154726205155 37.0 37.0 40.0 30.0 40.0 62-63 35.62977723083037 37.0 35.0 40.0 27.0 40.0 64-65 35.40658419198462 37.0 33.0 38.5 27.0 40.0 66-67 34.86871071267987 37.0 33.0 37.0 27.0 40.0 68-69 34.66705077086409 37.0 33.0 37.0 27.0 40.0 70-71 34.3570039129119 37.0 33.0 37.0 27.0 40.0 72-73 33.83784869093944 37.0 33.0 37.0 27.0 40.0 74-75 33.26900394873421 37.0 33.0 37.0 24.5 37.0 76-77 30.651584824789175 33.0 30.0 35.0 22.0 37.0 78-79 32.90879089721209 35.0 33.0 37.0 27.0 37.0 80-81 33.20084109779171 37.0 33.0 37.0 27.0 37.0 82-83 33.07364003204407 37.0 33.0 37.0 27.0 37.0 84-85 32.948516913961726 37.0 33.0 37.0 27.0 37.0 86-87 32.81334445402147 37.0 33.0 37.0 27.0 37.0 88-89 32.63935826352348 37.0 33.0 37.0 22.0 37.0 90-91 32.53451598224362 37.0 33.0 37.0 22.0 37.0 92-93 32.36920850766437 33.0 33.0 37.0 22.0 37.0 94-95 32.24818366505866 33.0 33.0 37.0 22.0 37.0 96-97 32.08810008078608 33.0 33.0 37.0 22.0 37.0 98-99 31.964004911036465 33.0 33.0 37.0 22.0 37.0 100-101 30.391717593774622 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1767715550387976 2306 2 0.18944387317641542 2306 3 0.13881387186150818 2306 4 0.13676936770546178 2306 5 0.1277845075320343 2306 6 0.2187784768924672 2306 7 0.18191096039713983 2306 8 0.17956671760441623 2306 9 0.15817988868082722 2306 10-11 0.2634056082289291 2306 12-13 0.24225162252439247 2306 14-15 0.39178506844095295 2306 16-17 0.47492871937036085 2306 18-19 0.5369615635786786 2306 20-21 0.49757345047920154 2306 22-23 0.4531537847981113 2306 24-25 0.41509189983997885 2306 26-27 0.4645845064052665 2306 28-29 0.5116235096551662 2306 30-31 0.5552455930475375 2306 32-33 0.4961937710287927 2306 34-35 0.5509449219012126 2306 36-37 0.4885505100990173 2306 38-39 0.5591739339028265 2306 40-41 0.6728177376957873 2306 42-43 0.4737951863895873 2306 44-45 0.5264267609728677 2306 46-47 0.5452661117579538 2306 48-49 0.4541037186481347 2306 50-51 0.4814195323268322 2306 52-53 0.45046803734668117 2306 54-55 0.6738708799914619 2306 56-57 0.6053325034646164 2306 58-59 0.5635317681294367 2306 60-61 0.5174946586335452 2306 62-63 0.5570918557619038 2306 64-65 0.5010936016914513 2306 66-67 0.6402184769720378 2306 68-69 0.5668394204308029 2306 70-71 0.4514696820970201 2306 72-73 0.29365273750536147 2306 74-75 0.4222819769310959 2306 76-77 0.10190835277055044 2306 78-79 0.35970646990648447 2306 80-81 0.5277229123952552 2306 82-83 0.4913577317764606 2306 84-85 0.5929794489750719 2306 86-87 0.6065656351360573 2306 88-89 0.5508430417032955 2306 90-91 0.4802649659969518 2306 92-93 0.4586742967634905 2306 94-95 0.5571543389958507 2306 96-97 0.5827571199423005 2306 98-99 0.5242596340935748 2306 100-101 0.3529362630943069 2307 1 0.14434163697530522 2307 2 0.21245571295989407 2307 3 0.1413361328523166 2307 4 0.15596931709831807 2307 5 0.12424945593066639 2307 6 0.12489369407678907 2307 7 0.16473124868083033 2307 8 0.2204753835814941 2307 9 0.16172163320671729 2307 10-11 0.2301595581878786 2307 12-13 0.20218726357810368 2307 14-15 0.2850810412219076 2307 16-17 0.37042501807514583 2307 18-19 0.46182916781612704 2307 20-21 0.426474054504844 2307 22-23 0.37843945392972245 2307 24-25 0.42476401238819506 2307 26-27 0.42732494162240187 2307 28-29 0.3143021691789727 2307 30-31 0.40585390432121216 2307 32-33 0.4546596674477925 2307 34-35 0.3857344251070103 2307 36-37 0.40048876052969007 2307 38-39 0.4048957625738865 2307 40-41 0.5116651232559448 2307 42-43 0.43255022794293296 2307 44-45 0.3665467197703549 2307 46-47 0.3025095832958584 2307 48-49 0.37976025136347147 2307 50-51 0.4038562285956573 2307 52-53 0.36237714117056896 2307 54-55 0.44933969776407423 2307 56-57 0.45554604014194666 2307 58-59 0.338686612176744 2307 60-61 0.30804455147119114 2307 62-63 0.32898457379524615 2307 64-65 0.25834117534439116 2307 66-67 0.40220267109103247 2307 68-69 0.27780048229134735 2307 70-71 0.24400704966617326 2307 72-73 0.12452153632187901 2307 74-75 0.18639892002634895 2307 76-77 -0.03769577045957817 2307 78-79 0.2730803796344716 2307 80-81 0.329228124132392 2307 82-83 0.3269758082429064 2307 84-85 0.3553177743664975 2307 86-87 0.381383835874999 2307 88-89 0.3759364943606798 2307 90-91 0.28312384870604035 2307 92-93 0.2304303226085409 2307 94-95 0.2883410679196743 2307 96-97 0.3813975170961186 2307 98-99 0.346231080350357 2307 100-101 0.07421951478475464 2201 1 0.1234849949104202 2201 2 0.17226624967973692 2201 3 0.1387874929309163 2201 4 0.15355970979842937 2201 5 0.09224251420967278 2201 6 0.11518006484297416 2201 7 0.11194563392272983 2201 8 0.14749360457894767 2201 9 0.17651243064624822 2201 10-11 0.24099011196179987 2201 12-13 0.17774426483973826 2201 14-15 0.2978670873600464 2201 16-17 0.2694492247621483 2201 18-19 0.3036857546468781 2201 20-21 0.30022806207643526 2201 22-23 0.3146551054760778 2201 24-25 0.36245327855974097 2201 26-27 0.26614095497419754 2201 28-29 0.4022736759442722 2201 30-31 0.4367485850700987 2201 32-33 0.44028961128532274 2201 34-35 0.37122570066427585 2201 36-37 0.40663942379836726 2201 38-39 0.35459371638970083 2201 40-41 0.5697958427759957 2201 42-43 0.36016990347810207 2201 44-45 0.33401782277269376 2201 46-47 0.4014780749187068 2201 48-49 0.3429810221958931 2201 50-51 0.3905859000662275 2201 52-53 0.3663264545986209 2201 54-55 0.5539967950806428 2201 56-57 0.4134147837286335 2201 58-59 0.37072925934036505 2201 60-61 0.35318213328583425 2201 62-63 0.3259322685279997 2201 64-65 0.25647531092801046 2201 66-67 0.40339304682741783 2201 68-69 0.4298432198116089 2201 70-71 0.2832185481594749 2201 72-73 0.311700178229664 2201 74-75 0.47058852088175485 2201 76-77 0.35891503977853034 2201 78-79 0.49818118921530896 2201 80-81 0.5333440435278689 2201 82-83 0.5659228970032899 2201 84-85 0.5867976891805995 2201 86-87 0.5595380364600118 2201 88-89 0.6222950857241258 2201 90-91 0.6074109531097776 2201 92-93 0.4928545414499652 2201 94-95 0.6011584182581231 2201 96-97 0.5432523288562976 2201 98-99 0.5232381889396507 2201 100-101 0.5034111042147238 1101 1 0.07123658262079857 1101 2 0.18155037183187517 1101 3 0.1257128773021563 1101 4 0.10915823846888628 1101 5 0.10386930702540553 1101 6 0.12913711309784048 1101 7 0.11703077143258866 1101 8 0.10997505582265177 1101 9 0.09798790956136827 1101 10-11 0.16756007224909553 1101 12-13 0.13798474643970593 1101 14-15 0.19677899892004547 1101 16-17 0.2837977851736184 1101 18-19 0.28042289004104504 1101 20-21 0.3136117172993451 1101 22-23 0.2656927046156454 1101 24-25 0.19168818191716497 1101 26-27 0.27202068847859096 1101 28-29 0.19235825044317778 1101 30-31 0.2925279088245034 1101 32-33 0.22542265252779004 1101 34-35 0.24111959894482737 1101 36-37 0.33403787321037015 1101 38-39 0.3054023043854599 1101 40-41 0.42517037397690416 1101 42-43 0.3050711805980697 1101 44-45 0.24417135237990806 1101 46-47 0.26246839714309544 1101 48-49 0.2547385814994598 1101 50-51 0.26663364010821766 1101 52-53 0.22726291983887847 1101 54-55 0.38715808887507563 1101 56-57 0.3471227410526154 1101 58-59 0.34942324175028716 1101 60-61 0.30654670020928876 1101 62-63 0.2520456556914965 1101 64-65 0.22501811699974184 1101 66-67 0.3043268014824321 1101 68-69 0.22344519610769709 1101 70-71 0.19445161830302737 1101 72-73 0.02091306208342303 1101 74-75 0.1149861897784561 1101 76-77 -0.08400107755771558 1101 78-79 0.30720088438997095 1101 80-81 0.4366913697275976 1101 82-83 0.4677144052406632 1101 84-85 0.3021883800722662 1101 86-87 0.41580981636499104 1101 88-89 0.2811933041999808 1101 90-91 0.2728163065113023 1101 92-93 0.27228042945971254 1101 94-95 0.2590782348554086 1101 96-97 0.25687811123714255 1101 98-99 0.2546489966477665 1101 100-101 0.1699043038079715 2304 1 0.13096833976864275 2304 2 0.1997361376458855 2304 3 0.11109493964976025 2304 4 0.08664896912482334 2304 5 0.1072972257256879 2304 6 0.10167543213631092 2304 7 0.13816531395602993 2304 8 0.1304722026005507 2304 9 0.1328554829289601 2304 10-11 0.18223947697403986 2304 12-13 0.12300402985137993 2304 14-15 0.18286567378962104 2304 16-17 0.2028961658927173 2304 18-19 0.3455805879910301 2304 20-21 0.29356208914089876 2304 22-23 0.26564561180283164 2304 24-25 0.16233347939862597 2304 26-27 0.10733657447236311 2304 28-29 0.2620625951932283 2304 30-31 0.3425822298364949 2304 32-33 0.3285752151708152 2304 34-35 0.285288824229724 2304 36-37 0.22309774734114285 2304 38-39 0.2641776119809265 2304 40-41 0.44184944357625255 2304 42-43 0.34031166333225116 2304 44-45 0.31427762881143195 2304 46-47 0.30406983205015337 2304 48-49 0.30438029280846735 2304 50-51 0.2569661975053208 2304 52-53 0.2532812882262334 2304 54-55 0.4709019784457169 2304 56-57 0.5729897279504073 2304 58-59 0.3915669284932761 2304 60-61 0.4384105461259864 2304 62-63 0.3097260006299294 2304 64-65 0.27074304788008163 2304 66-67 0.3759707130114691 2304 68-69 0.34748465331374945 2304 70-71 0.2686902890591014 2304 72-73 0.1150040913954129 2304 74-75 0.20635733535493017 2304 76-77 0.04663178413562008 2304 78-79 0.3290534554111346 2304 80-81 0.4328572755167812 2304 82-83 0.41519209228263776 2304 84-85 0.3884207962456827 2304 86-87 0.4183113482503842 2304 88-89 0.4463861301027805 2304 90-91 0.34210653568354843 2304 92-93 0.26287856841746304 2304 94-95 0.3671110640218487 2304 96-97 0.411874384881429 2304 98-99 0.4690510739884033 2304 100-101 0.4887288069651028 2202 1 0.10462058252416284 2202 2 0.12349418085997144 2202 3 0.07824418119150778 2202 4 0.08408759026718116 2202 5 0.09810223934051265 2202 6 0.09647015595751185 2202 7 0.08943586108938462 2202 8 0.10235346111925026 2202 9 0.040131828781376555 2202 10-11 0.16531913072426363 2202 12-13 0.12489645567712415 2202 14-15 0.042951773324425346 2202 16-17 0.11542706179740492 2202 18-19 0.22435607724716533 2202 20-21 0.11680656992798077 2202 22-23 0.12767075146964402 2202 24-25 0.19660879549537924 2202 26-27 0.017781327426611426 2202 28-29 0.1320196065239898 2202 30-31 0.1240237411377123 2202 32-33 0.10679092303475812 2202 34-35 0.16711940146075932 2202 36-37 0.12871121676955255 2202 38-39 0.13948531312435364 2202 40-41 0.24263851560149874 2202 42-43 0.11619086952763524 2202 44-45 0.1629836199825334 2202 46-47 0.07453491416105607 2202 48-49 0.08893044282962137 2202 50-51 0.1594902202257913 2202 52-53 0.18347817958031953 2202 54-55 0.268380140883167 2202 56-57 0.37868965487700024 2202 58-59 0.3487260449361713 2202 60-61 0.2362074612867957 2202 62-63 0.21454512202679865 2202 64-65 0.14941157940652516 2202 66-67 0.178713237147754 2202 68-69 0.19692378653060416 2202 70-71 0.10739875147130107 2202 72-73 0.0506898262963702 2202 74-75 0.059321904600643904 2202 76-77 -0.1354596535099617 2202 78-79 0.1263472092091078 2202 80-81 0.2258185583999932 2202 82-83 0.2559571548852517 2202 84-85 0.18437380471216613 2202 86-87 0.26697909915790774 2202 88-89 0.31716875734976924 2202 90-91 0.12024640994208369 2202 92-93 0.039586265736858195 2202 94-95 0.09679211434675494 2202 96-97 0.25625146320741266 2202 98-99 0.21339996090201652 2202 100-101 0.2605482557043892 1102 1 0.09708764827266236 1102 2 0.16105452584203306 1102 3 0.09612209414789419 1102 4 0.06762511205607069 1102 5 0.04160347075296755 1102 6 0.011797286446693533 1102 7 0.04418567299001808 1102 8 0.0397518570918578 1102 9 0.06292931789053569 1102 10-11 0.12911828227547772 1102 12-13 0.07926085765938495 1102 14-15 0.08358533078867225 1102 16-17 0.09089433042679218 1102 18-19 0.19004694369483133 1102 20-21 0.17288497979822637 1102 22-23 0.03670835651150384 1102 24-25 0.047456801340068466 1102 26-27 0.06076767897266677 1102 28-29 0.1379978645121014 1102 30-31 0.1932014406967042 1102 32-33 0.18866748595745975 1102 34-35 0.04986376503993739 1102 36-37 0.06699776188800399 1102 38-39 0.13628913388879482 1102 40-41 0.170532985865961 1102 42-43 0.015337494688388631 1102 44-45 0.0870376652212741 1102 46-47 -0.010884614711017093 1102 48-49 0.1147346695571585 1102 50-51 0.13037733382680017 1102 52-53 0.1368060468297898 1102 54-55 0.2106258496974931 1102 56-57 0.19037995596955426 1102 58-59 0.12843009119167448 1102 60-61 0.0764179117796644 1102 62-63 0.023759628727951565 1102 64-65 0.06083776623450632 1102 66-67 0.1497537529900086 1102 68-69 0.14908706852717302 1102 70-71 0.05275626241616038 1102 72-73 -0.14633700572370145 1102 74-75 -0.1183734934126548 1102 76-77 -0.276217258237736 1102 78-79 0.029566300628921738 1102 80-81 0.1343293811856583 1102 82-83 0.06361876380245235 1102 84-85 -0.044310448006676495 1102 86-87 0.06680246259273304 1102 88-89 0.04539860857023115 1102 90-91 0.06379789178794226 1102 92-93 -0.09301451079424794 1102 94-95 -0.1405632994688304 1102 96-97 -0.011585611834149745 1102 98-99 0.15411109362631947 1102 100-101 0.19923245876003293 2305 1 0.10022888951699827 2305 2 0.14150926966291877 2305 3 0.07772289164356039 2305 4 0.05168961953133078 2305 5 0.04785084554760033 2305 6 -0.0472986290328663 2305 7 0.025699189294783764 2305 8 0.04611293043639364 2305 9 0.04317294324641807 2305 10-11 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-1.6249681815711412 1213 98-99 -1.6130878859015176 1213 100-101 -1.5155232927788767 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 30.0 10 41.0 11 49.0 12 55.0 13 162.0 14 396.0 15 1032.0 16 2009.0 17 3429.0 18 5245.0 19 7668.0 20 10178.0 21 13775.0 22 18037.0 23 23299.0 24 30265.0 25 38422.0 26 51171.0 27 71714.0 28 113296.0 29 152091.0 30 125069.0 31 122508.0 32 161810.0 33 229578.0 34 352843.0 35 609153.0 36 1198395.0 37 1975298.0 38 600566.0 39 509.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.13951712037159 25.279156269796932 22.11103831492263 15.47028829490885 2 28.957896784085563 25.870570938404374 31.50896934978614 13.66256292772392 3 35.92399033184274 19.41372264609029 28.181049492529887 16.481237529537086 4 28.063175914605036 19.873577194211354 32.02208047561068 20.041166415572935 5 34.68105214191246 18.39004382995283 30.276090345903306 16.652813682231404 6 28.905160255007562 19.444656130287783 34.8315058602433 16.818677754461355 7 31.14048953895499 23.1041082617915 27.51233124014992 18.24307095910359 8 27.803539616353156 29.217236818782517 25.333438997894426 17.645784566969898 9 32.0369767386453 23.61545489522903 25.774357610442138 18.57321075568354 10-11 24.5395016203683 30.063589676347085 29.120575711896674 16.276332991387942 12-13 22.71623877870242 28.47323101556767 29.12677702755564 19.683753178174275 14-15 21.93581925547376 28.77656321734395 29.6699328618869 19.617684665295393 16-17 23.179064763870965 27.309132544082143 30.31981215589668 19.191990536150204 18-19 20.73180087051602 27.48878482769552 32.30079456257829 19.478619739210178 20-21 22.76370451208425 27.73299658091077 31.884339837986758 17.61895906901822 22-23 20.881529866114665 31.58763394215343 29.69954900118476 17.831287190547148 24-25 20.732544352502384 27.40502482300482 31.742524077410668 20.119906747082126 26-27 20.682232588538987 27.69171316667734 29.479322667633646 22.146731577150028 28-29 22.299973352929715 29.644781204234672 29.42762532360476 18.627620119230855 30-31 20.00150724075417 28.050282700576656 31.32902136311001 20.619188695559167 32-33 19.99450330249629 30.064840077869597 29.694805713794242 20.245850905839866 34-35 22.296399570199824 29.84763350528607 29.75430961867992 18.101657305834188 36-37 20.241596328928086 29.675306247231457 31.822304411408243 18.260793012432213 38-39 20.85290826819057 27.524071414093715 31.09663484744372 20.526385470271997 40-41 22.562698571422175 27.785884759862235 30.697478437075244 18.953938231640347 42-43 20.41846593189336 29.817892251084626 31.10183045577631 18.661811361245707 44-45 20.182425801862024 27.802217717151294 31.418594640354954 20.596761840631732 46-47 22.2816002600873 27.688964184431498 29.356779040532693 20.672656514948514 48-49 20.409899093351683 30.0056582614546 30.74143502533967 18.843007619854053 50-51 22.709967423385645 27.93008303919079 30.241562717713222 19.11838681971035 52-53 20.59558447073922 27.766119262401435 28.382564847281994 23.255731419577355 54-55 22.643168686396713 28.019887066840184 30.072279960385416 19.264664286377684 56-57 20.548387091321903 28.198803567628616 30.254707954451533 20.998101386597952 58-59 20.52617955964301 28.206437588460158 30.537412716562347 20.729970135334487 60-61 22.240599958385992 28.474528829532492 30.419687921982018 18.8651832900995 62-63 20.177898333863375 28.29727690975068 30.387121009597884 21.13770374678806 64-65 22.124450955518853 28.05094162770908 30.320994409828778 19.50361300694329 66-67 20.385344291014928 31.297635089596334 28.538092648440205 19.778927970948537 68-69 20.218703736349397 31.71775461054066 28.545928443807206 19.517613209302734 70-71 19.942666675681338 32.392111259102 28.272056089299358 19.3931659759173 72-73 19.933334550318797 32.21039379959427 28.087686507248332 19.7685851428386 74-75 20.20076120081791 31.466790245655503 28.368114152907363 19.964334400619226 76-77 20.102271183163662 31.183136620496217 28.537822744145767 20.17676945219435 78-79 19.933390204696348 31.06708331023779 28.685287982251808 20.31423850281405 80-81 19.84903436597991 31.157396152504884 28.449710245996634 20.543859235518575 82-83 19.54671372605481 30.872592722524512 28.60992130463062 20.970772246790055 84-85 19.71416681629506 30.6550935265476 28.84496033967406 20.785779317483286 86-87 20.023596596241703 30.513458384588176 29.10076431166945 20.362180707500666 88-89 20.28655798168108 30.58905603733682 28.691474054622805 20.432911926359292 90-91 20.322567315183186 30.456716425730313 28.570641467959558 20.650074791126944 92-93 19.89480601308467 30.9020523004676 28.695208436211523 20.507933250236217 94-95 19.58364188451225 31.085330885212198 28.731254459198546 20.599772771077003 96-97 19.80265193071307 31.206486479176565 28.568527086443 20.422334503667365 98-99 20.353272212690168 30.666398242259618 28.165825431674367 20.814504113375843 100-101 20.491085098321566 30.575142433136865 27.660692240553892 21.273080227987677 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 134.0 1 81.0 2 42.5 3 73.0 4 73.0 5 61.5 6 67.0 7 68.0 8 61.5 9 61.5 10 64.5 11 71.5 12 84.5 13 101.0 14 125.0 15 150.5 16 184.5 17 242.5 18 328.0 19 492.0 20 755.0 21 1190.0 22 2017.5 23 3286.0 24 5117.0 25 7898.0 26 12441.0 27 18934.0 28 26523.5 29 35463.5 30 46095.5 31 57788.5 32 75371.0 33 103765.5 34 140622.0 35 187966.0 36 255477.0 37 326594.0 38 386807.0 39 418298.5 40 425174.0 41 414866.0 42 386113.0 43 359539.5 44 325647.0 45 292591.0 46 273031.0 47 245918.0 48 210002.0 49 180851.0 50 151530.0 51 124136.0 52 100985.5 53 79107.0 54 63014.0 55 49662.0 56 36187.5 57 24832.5 58 17512.5 59 12761.5 60 9211.5 61 6580.5 62 4479.0 63 2976.0 64 2000.0 65 1355.0 66 930.5 67 666.5 68 471.5 69 303.0 70 197.0 71 151.0 72 120.0 73 75.0 74 49.0 75 39.5 76 33.5 77 23.5 78 16.0 79 12.0 80 12.0 81 9.5 82 4.0 83 2.5 84 1.5 85 1.5 86 0.5 87 0.5 88 1.0 89 2.0 90 1.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16339706382066269 2 0.0 3 0.0019431915552612416 4 0.011050845888181324 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.224329467959221E-5 22-23 2.1121647339796107E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.688279631685782E-4 38-39 0.002289586571633898 40-41 0.006091483092797197 42-43 0.002940133309699618 44-45 0.0012926448171955217 46-47 0.0014531693369779723 48-49 0.003438604186918806 50-51 8.448658935918443E-4 52-53 0.014692217889562173 54-55 0.026875184075156565 56-57 0.02831990475319862 58-59 0.020961122820013656 60-61 0.030786913162486808 62-63 0.00569439612280903 64-65 0.011143781136476426 66-67 0.028750786358930462 68-69 0.02706950323068269 70-71 0.030736221208871296 72-73 0.031107962202051705 74-75 0.03910884221436647 76-77 0.019989527042383038 78-79 0.011000153934565812 80-81 0.0156215703725132 82-83 0.007941739399763337 84-85 0.0111775757722201 86-87 0.03365100854176316 88-89 0.015832786845911162 90-91 0.021755296759989988 92-93 0.04954293600022575 94-95 0.056758090731500094 96-97 0.04070563875325506 98-99 0.035391432282562356 100-101 0.03510417787874113 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5918099.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.1396678076779 #Duplication Level Percentage of deduplicated Percentage of total 1 90.89888576964164 49.21235479656459 2 4.523232199328047 4.897725773772256 3 1.3062965807818254 2.1216738882550055 4 0.7392763014356454 1.6009669351125837 5 0.3609958864655564 0.9772098686591717 6 0.30805714724732597 1.0006866970650687 7 0.23121634528630208 0.8762583287854022 8 0.18406016111150517 0.7971964783363652 9 0.13800253462841666 0.6724270243260041 >10 1.0666260182167355 11.568066410965448 >50 0.1348730176349634 5.001026698830307 >100 0.0915846898560483 9.838452078257868 >500 0.01396662860021351 5.294781537700314 >1k 0.002800944611839091 2.1404834840010487 >5k 0.0 0.0 >10k+ 1.2577515406681145E-4 4.000689999368555 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 203030 3.430662447519043 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 11796 0.19932076161618792 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 10099 0.17064601318768072 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 10084 0.17039255341960316 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.265846684889861E-4 0.0 0.0 2 7.772766221044967E-4 0.0 0.0 3 8.279685757200074E-4 0.0 1.6897317871836886E-5 4 9.293524829510287E-4 0.0 1.6897317871836886E-5 5 0.0010814283437975607 0.0 3.379463574367377E-5 6 0.0011659149331567451 0.0 3.379463574367377E-5 7 0.001334888111875114 0.0 3.379463574367377E-5 8 0.001622142515696341 0.0 3.379463574367377E-5 9 0.0018249103301583836 0.0 3.379463574367377E-5 10-11 0.0031429011241616606 0.0 6.758927148734754E-5 12-13 0.004452443259229019 0.0 8.448658935918442E-5 14-15 0.005871817960463318 0.0 1.0138390723102131E-4 16-17 0.006902554350645368 0.0 1.0138390723102131E-4 18-19 0.008330377710815585 0.0 1.0138390723102131E-4 20-21 0.010265120607140908 0.0 1.0138390723102131E-4 22-23 0.012301247410697252 0.0 1.0983256616693975E-4 24-25 0.014785153137857275 0.0 1.3517854297469508E-4 26-27 0.01769994047074914 0.0 1.436272019106135E-4 28-29 0.021172339293411616 0.0 1.5207586084653197E-4 30-31 0.02978997140804843 0.0 1.5207586084653197E-4 32-33 0.03373549513112234 0.0 1.6052451978245042E-4 34-35 0.03757963494696523 0.0 1.7742183765428728E-4 36-37 0.04186310502747588 0.0 1.8587049659020573E-4 38-39 0.04670418659775715 0.0 1.8587049659020573E-4 40-41 0.05180717659505189 0.0 1.8587049659020573E-4 42-43 0.0564370416919352 0.0 2.0276781446204261E-4 44-45 0.062156783791551984 0.0 2.0276781446204261E-4 46-47 0.06783428259648917 0.0 2.0276781446204261E-4 48-49 0.07431440400033862 0.0 2.0276781446204261E-4 50-51 0.08063400088440562 0.0 2.1121647339796107E-4 52-53 0.0874520686456918 0.0 2.1966513233387952E-4 54-55 0.09468412069483799 0.0 2.1966513233387952E-4 56-57 0.10231325971397234 0.0 2.1966513233387952E-4 58-59 0.11028034509054344 0.0 2.1966513233387952E-4 60-61 0.11915988563219372 0.0 2.1966513233387952E-4 62-63 0.1286899729119097 0.0 2.1966513233387952E-4 64-65 0.1384397253239596 0.0 2.450111091416348E-4 66-67 0.14891606240449848 0.0 2.5345976807755326E-4 68-69 0.16085401748095124 0.0 2.5345976807755326E-4 70-71 0.17392409285481708 0.0 2.5345976807755326E-4 72-73 0.18899650039649557 0.0 2.5345976807755326E-4 74-75 0.20539534739111326 0.0 2.5345976807755326E-4 76-77 0.22454000853990447 0.0 2.7035708594939017E-4 78-79 0.24551802867778993 0.0 2.7035708594939017E-4 80-81 0.2688701219766685 0.0 2.7035708594939017E-4 82-83 0.2949680294297206 0.0 3.0415172169306393E-4 84-85 0.3252902663507319 0.0 3.0415172169306393E-4 86-87 0.3592707725909958 0.0 3.0415172169306393E-4 88-89 0.39848944737152925 0.0 3.0415172169306393E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 26825 0.0 82.4034 3 CGGAAGA 26790 0.0 81.92461 4 GATCGGA 27135 0.0 81.611206 1 ATCGGAA 27510 0.0 80.2307 2 GAAGAGC 28690 0.0 76.30801 6 GGAAGAG 32315 0.0 68.1007 5 AGAGCAC 32755 0.0 66.85244 8 GAGCACA 32885 0.0 66.54484 9 AAGAGCA 34875 0.0 62.70687 7 GCCGTCT 23690 0.0 45.639694 50-51 ATGCCGT 23745 0.0 45.36086 48-49 TATGCCG 23765 0.0 45.292706 48-49 TCGTATG 24090 0.0 45.284122 44-45 TGCCGTC 23780 0.0 45.147335 50-51 CGTATGC 23910 0.0 45.082806 46-47 TCTCGTA 24030 0.0 45.050945 42-43 CGTATCT 23755 0.0 45.03383 38-39 CGTCTTC 24110 0.0 44.914364 52-53 AATTCGT 23655 0.0 44.84255 34-35 TCGTATC 23585 0.0 44.83487 38-39 >>END_MODULE