FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_35011-1.341000000358a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_35011-1.341000000358a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5918099
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2030303.430662447519043TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC117960.19932076161618792No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG100990.17064601318768072No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA100840.17039255341960316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG268250.082.40343
CGGAAGA267900.081.924614
GATCGGA271350.081.6112061
ATCGGAA275100.080.23072
GAAGAGC286900.076.308016
GGAAGAG323150.068.10075
AGAGCAC327550.066.852448
GAGCACA328850.066.544849
AAGAGCA348750.062.706877
GCCGTCT236900.045.63969450-51
ATGCCGT237450.045.3608648-49
TATGCCG237650.045.29270648-49
TCGTATG240900.045.28412244-45
TGCCGTC237800.045.14733550-51
CGTATGC239100.045.08280646-47
TCTCGTA240300.045.05094542-43
CGTATCT237550.045.0338338-39
CGTCTTC241100.044.91436452-53
AATTCGT236550.044.8425534-35
TCGTATC235850.044.8348738-39
Produced by FastQC (version 0.11.2)