FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_35010-1.341000000357eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_35010-1.341000000357eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6764852
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT712811.0536963705931779TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG147950.053.9644283
CGGAAGA150250.053.137174
GATCGGA152800.052.3285331
ATCGGAA155500.051.252662
GAAGAGC173350.045.9669726
TATGCCG94350.041.51036548-49
GCCGTCT95450.041.18554350-51
TGCCGTC95450.040.83714750-51
CGTATGC96100.040.74872646-47
ATGCCGT96650.040.645448-49
CTCGTAT96500.040.33090244-45
TCGTATG98550.040.2148144-45
TCTCGTA97450.040.0594242-43
CCGTCTT98200.039.7911852-53
CATCTCG97150.039.45043640-41
CGTCTTC100700.039.15714652-53
GTATGCC102200.038.85099446-47
ATCTCGT100150.038.29184342-43
CGAGATT102100.038.21058332-33
TCACGAG101300.038.08859330-31
Produced by FastQC (version 0.11.2)