##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_35008-2.341000000358b3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5694438 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67949989094622 33.0 33.0 33.0 33.0 33.0 2 32.66179384163986 33.0 33.0 33.0 33.0 33.0 3 32.73137243745564 33.0 33.0 33.0 33.0 33.0 4 36.682104537796356 37.0 37.0 37.0 37.0 37.0 5 36.687977110296046 37.0 37.0 37.0 37.0 37.0 6 36.57480018221289 37.0 37.0 37.0 37.0 37.0 7 36.50267190546284 37.0 37.0 37.0 37.0 37.0 8 36.44808214612223 37.0 37.0 37.0 37.0 37.0 9 36.478205750945044 37.0 37.0 37.0 37.0 37.0 10-11 36.338643602757635 37.0 37.0 37.0 37.0 37.0 12-13 36.37167636209227 37.0 37.0 37.0 37.0 37.0 14-15 38.28818989687832 40.0 37.0 40.0 37.0 40.0 16-17 38.25609963617129 40.0 37.0 40.0 37.0 40.0 18-19 38.149997769753575 40.0 37.0 40.0 37.0 40.0 20-21 38.16693877429169 40.0 37.0 40.0 37.0 40.0 22-23 38.06006826310164 40.0 37.0 40.0 35.0 40.0 24-25 38.06686059273979 40.0 37.0 40.0 37.0 40.0 26-27 38.0141659282268 40.0 37.0 40.0 35.0 40.0 28-29 37.85245594034038 40.0 37.0 40.0 33.0 40.0 30-31 37.75874686843548 40.0 37.0 40.0 33.0 40.0 32-33 37.65581335682292 40.0 37.0 40.0 33.0 40.0 34-35 37.53223109989081 40.0 37.0 40.0 33.0 40.0 36-37 37.36139246752708 40.0 37.0 40.0 33.0 40.0 38-39 37.38738001888861 40.0 37.0 40.0 33.0 40.0 40-41 37.16466005951773 40.0 37.0 40.0 33.0 40.0 42-43 37.112726488549 40.0 37.0 40.0 33.0 40.0 44-45 37.0761614227778 40.0 37.0 40.0 33.0 40.0 46-47 37.02926013067488 40.0 37.0 40.0 33.0 40.0 48-49 36.83841926104033 37.0 37.0 40.0 33.0 40.0 50-51 36.752712295752445 37.0 37.0 40.0 33.0 40.0 52-53 36.619694691556916 37.0 37.0 40.0 33.0 40.0 54-55 36.236722219119784 37.0 37.0 40.0 33.0 40.0 56-57 36.14434058286349 37.0 37.0 40.0 33.0 40.0 58-59 35.99866738385772 37.0 37.0 40.0 33.0 40.0 60-61 35.762480775100194 37.0 37.0 40.0 27.0 40.0 62-63 35.57165412987199 37.0 35.0 40.0 27.0 40.0 64-65 35.380833016357364 37.0 33.0 40.0 27.0 40.0 66-67 34.87586255219567 37.0 33.0 40.0 27.0 40.0 68-69 34.682798460532894 37.0 33.0 37.0 27.0 40.0 70-71 34.37544108830406 37.0 33.0 37.0 27.0 40.0 72-73 33.87427995528268 37.0 33.0 37.0 27.0 40.0 74-75 33.310008555717 37.0 33.0 37.0 22.0 40.0 76-77 30.73682969241214 33.0 30.0 35.0 22.0 37.0 78-79 32.91302513083819 37.0 33.0 37.0 22.0 37.0 80-81 33.15879257970673 37.0 33.0 37.0 24.5 37.0 82-83 32.97780509683309 37.0 33.0 37.0 22.0 37.0 84-85 32.81232722175569 37.0 33.0 37.0 22.0 37.0 86-87 32.64724604956626 37.0 33.0 37.0 22.0 37.0 88-89 32.43304405105473 37.0 33.0 37.0 22.0 37.0 90-91 32.29623581817907 37.0 33.0 37.0 22.0 37.0 92-93 32.113260430616684 33.0 33.0 37.0 22.0 37.0 94-95 31.96982081462648 33.0 33.0 37.0 22.0 37.0 96-97 31.80647247366641 33.0 33.0 37.0 22.0 37.0 98-99 31.66517011863155 33.0 33.0 37.0 15.0 37.0 100-101 30.047901917625587 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14917645001940372 2306 2 0.17872109435641192 2306 3 0.12663222986583378 2306 4 0.14671462162613835 2306 5 0.1271669605409329 2306 6 0.207549229021474 2306 7 0.23542258469615263 2306 8 0.1777458363660287 2306 9 0.1974285255927768 2306 10-11 0.23996243642597648 2306 12-13 0.24052386477450938 2306 14-15 0.3832642590861397 2306 16-17 0.44975439108954873 2306 18-19 0.5282585224073202 2306 20-21 0.5030533796190042 2306 22-23 0.5005607129838126 2306 24-25 0.4981713454394239 2306 26-27 0.5136433453911948 2306 28-29 0.5297445118330586 2306 30-31 0.6544373374124532 2306 32-33 0.6287222108836374 2306 34-35 0.5719503312684893 2306 36-37 0.5735442250698455 2306 38-39 0.6271973169941063 2306 40-41 0.8174158435515864 2306 42-43 0.7081724056943202 2306 44-45 0.6594773450166329 2306 46-47 0.6261696945106436 2306 48-49 0.49273167419833186 2306 50-51 0.6078804602045196 2306 52-53 0.646976650463877 2306 54-55 0.7811197608394025 2306 56-57 0.7567781837361665 2306 58-59 0.6213858849677365 2306 60-61 0.6608151251424559 2306 62-63 0.6807774681379399 2306 64-65 0.5961258860518797 2306 66-67 0.6712135016575544 2306 68-69 0.5491356089537689 2306 70-71 0.4725822056054412 2306 72-73 0.3403723696486267 2306 74-75 0.5118963875912286 2306 76-77 0.23417443866502197 2306 78-79 0.5158149829689336 2306 80-81 0.697150307989368 2306 82-83 0.6879530871788759 2306 84-85 0.6116021606505981 2306 86-87 0.7206997071139014 2306 88-89 0.6114731465473255 2306 90-91 0.6810884329863498 2306 92-93 0.6585062304459726 2306 94-95 0.6238371371710656 2306 96-97 0.5663533793403701 2306 98-99 0.7310432321139437 2306 100-101 0.5742193664419979 2307 1 0.1295387761257487 2307 2 0.16803796322948017 2307 3 0.11517523825489207 2307 4 0.12347566842027646 2307 5 0.060813437418957506 2307 6 0.1423316868126605 2307 7 0.14560244941196743 2307 8 0.1649074652266549 2307 9 0.1659753390101244 2307 10-11 0.17069073940906065 2307 12-13 0.17263463994618178 2307 14-15 0.3028755805878873 2307 16-17 0.3415399006162616 2307 18-19 0.4330970143820494 2307 20-21 0.3717262345985759 2307 22-23 0.4160170335728708 2307 24-25 0.35010393987739263 2307 26-27 0.28832338878908814 2307 28-29 0.30574487433913333 2307 30-31 0.43782793899256944 2307 32-33 0.4582959577309893 2307 34-35 0.43772120943302895 2307 36-37 0.43095830984484707 2307 38-39 0.42266155615555334 2307 40-41 0.4433173524509826 2307 42-43 0.40016243549180075 2307 44-45 0.43823564602008247 2307 46-47 0.36464605063044075 2307 48-49 0.43634314738702784 2307 50-51 0.4723856522684642 2307 52-53 0.46074159741201726 2307 54-55 0.49037337111708723 2307 56-57 0.5631103421384651 2307 58-59 0.46087693672383523 2307 60-61 0.3863276470277981 2307 62-63 0.2869693161219189 2307 64-65 0.233458162718577 2307 66-67 0.2997927992184728 2307 68-69 0.23851675195341926 2307 70-71 0.18811134877991975 2307 72-73 0.13454039249936756 2307 74-75 0.22835191375208552 2307 76-77 -0.018695079716096075 2307 78-79 0.26063005345194057 2307 80-81 0.44424736706243806 2307 82-83 0.34872575216413537 2307 84-85 0.3535659023303097 2307 86-87 0.4029283610337231 2307 88-89 0.45295200421612947 2307 90-91 0.41065922322388104 2307 92-93 0.37559492102775494 2307 94-95 0.36147960077262553 2307 96-97 0.4689976811819605 2307 98-99 0.3577330631758322 2307 100-101 0.16783739559160082 2201 1 0.15579594242019112 2201 2 0.17285819575931072 2201 3 0.11619771871718143 2201 4 0.11029389626492048 2201 5 0.09820354665268383 2201 6 0.12482459589301698 2201 7 0.16431292030819122 2201 8 0.11611738716680264 2201 9 0.09964039730186869 2201 10-11 0.18332713934945133 2201 12-13 0.1547403785566246 2201 14-15 0.28432808991445313 2201 16-17 0.23634967738171042 2201 18-19 0.2777614457830211 2201 20-21 0.31312503155992033 2201 22-23 0.30061804774788925 2201 24-25 0.2599142684432465 2201 26-27 0.19984805312946463 2201 28-29 0.2599570455770177 2201 30-31 0.36385363355000067 2201 32-33 0.4415246954960921 2201 34-35 0.3311418144565721 2201 36-37 0.3830359237006178 2201 38-39 0.4265231350328591 2201 40-41 0.523321471043694 2201 42-43 0.39763780642113744 2201 44-45 0.38672485064246587 2201 46-47 0.3127892138234216 2201 48-49 0.3485324183392251 2201 50-51 0.30444542647401107 2201 52-53 0.4146841259776437 2201 54-55 0.5169186113550523 2201 56-57 0.4007896061618723 2201 58-59 0.3446260734690796 2201 60-61 0.26446963835599746 2201 62-63 0.2646889810368549 2201 64-65 0.14167754263144872 2201 66-67 0.2824787246673921 2201 68-69 0.3427353513815916 2201 70-71 0.23367128966331308 2201 72-73 0.3070756366170073 2201 74-75 0.3907461472671869 2201 76-77 0.21339330846923232 2201 78-79 0.4971293179076923 2201 80-81 0.4275727552284181 2201 82-83 0.5008606508660947 2201 84-85 0.5457364803326996 2201 86-87 0.5372526217065143 2201 88-89 0.5222947132261737 2201 90-91 0.4998825044225086 2201 92-93 0.36533524489477287 2201 94-95 0.42208782134716216 2201 96-97 0.4720175084513585 2201 98-99 0.3473883984031083 2201 100-101 0.29103389000931656 1101 1 0.08256406638425062 1101 2 0.16782435528238437 1101 3 0.10141766369992666 1101 4 0.10959007926081199 1101 5 0.10612170484559869 1101 6 0.13031284769650142 1101 7 0.15476211692795516 1101 8 0.15230223663182585 1101 9 0.10830575183711488 1101 10-11 0.15358327954167095 1101 12-13 0.134550026958415 1101 14-15 0.28178687724658147 1101 16-17 0.305366255535823 1101 18-19 0.33311353239553654 1101 20-21 0.32570560102549706 1101 22-23 0.28742113443786366 1101 24-25 0.2605738130051165 1101 26-27 0.2679292196478471 1101 28-29 0.32065476803928306 1101 30-31 0.388032000814988 1101 32-33 0.30749389555354156 1101 34-35 0.3899620281258436 1101 36-37 0.41035385781118094 1101 38-39 0.44694469850382745 1101 40-41 0.5444869136134898 1101 42-43 0.48285279084898036 1101 44-45 0.44605196463754737 1101 46-47 0.37260574882274256 1101 48-49 0.4013774088575204 1101 50-51 0.40745910648005434 1101 52-53 0.4130180531826255 1101 54-55 0.5466693353051753 1101 56-57 0.5841333488352305 1101 58-59 0.5134888744208936 1101 60-61 0.4418931986034167 1101 62-63 0.42194234831971045 1101 64-65 0.3361350462413739 1101 66-67 0.6239692448530221 1101 68-69 0.5438087909681073 1101 70-71 0.40091632198909366 1101 72-73 0.2858165967456827 1101 74-75 0.368061432686396 1101 76-77 0.11206376747672664 1101 78-79 0.5547832397928332 1101 80-81 0.6735865768042046 1101 82-83 0.6656387767786569 1101 84-85 0.643242438867567 1101 86-87 0.7143102644773549 1101 88-89 0.5105819856977192 1101 90-91 0.6106028045544534 1101 92-93 0.48161032032123074 1101 94-95 0.38803447945907976 1101 96-97 0.5731247175768246 1101 98-99 0.5771290991743747 1101 100-101 0.5256907456646154 2304 1 0.11613059775380208 2304 2 0.15749678282445956 2304 3 0.11366817552332265 2304 4 0.09156745640170527 2304 5 0.09936930406414746 2304 6 0.09426147345229907 2304 7 0.12150667685497041 2304 8 0.13867564204881688 2304 9 0.09454968070889436 2304 10-11 0.04492762385432769 2304 12-13 0.0888367113545101 2304 14-15 0.16664698952484258 2304 16-17 0.16867554339926727 2304 18-19 0.3131288665163723 2304 20-21 0.19788867436602686 2304 22-23 0.14510688228543245 2304 24-25 0.1175198386194225 2304 26-27 0.15932917804705227 2304 28-29 0.2189798235939051 2304 30-31 0.2376674038277713 2304 32-33 0.16122596664224886 2304 34-35 0.1296527497753246 2304 36-37 0.1368910247600894 2304 38-39 0.06457020659978951 2304 40-41 0.3171400266028357 2304 42-43 0.22360434205208435 2304 44-45 0.21923830512272247 2304 46-47 0.2205638794649829 2304 48-49 0.21933656488449316 2304 50-51 0.09242879131465287 2304 52-53 0.21104609932987017 2304 54-55 0.42687750761224663 2304 56-57 0.45098455883493926 2304 58-59 0.28049301858504094 2304 60-61 0.3284383811154967 2304 62-63 0.21475250341532615 2304 64-65 0.12099764651823364 2304 66-67 0.20475705223290674 2304 68-69 0.2392862909539275 2304 70-71 0.20486864041047426 2304 72-73 0.02106930514254657 2304 74-75 0.0840330437075707 2304 76-77 0.04197869896255213 2304 78-79 0.27452437451410816 2304 80-81 0.300035401508417 2304 82-83 0.28276965794544395 2304 84-85 0.3375791183151051 2304 86-87 0.4013183702304701 2304 88-89 0.3194409895360053 2304 90-91 0.3371785720700089 2304 92-93 0.1624537234935346 2304 94-95 0.26312425779120474 2304 96-97 0.32161758040523125 2304 98-99 0.22826346646626305 2304 100-101 0.2871966415810263 2202 1 0.08728115575052442 2202 2 0.11671379900861467 2202 3 0.09757769598208199 2202 4 0.06274099614173423 2202 5 0.06530359132089814 2202 6 0.1015684978430329 2202 7 0.05549901515959732 2202 8 0.07442478838152056 2202 9 0.05791226910503866 2202 10-11 0.12822708349086298 2202 12-13 0.12791194271798645 2202 14-15 0.11742448279327533 2202 16-17 0.12385759223422355 2202 18-19 0.2521610593423915 2202 20-21 0.17130117910862452 2202 22-23 0.10629146997624161 2202 24-25 0.11377774224987292 2202 26-27 0.08408385838352928 2202 28-29 0.11343286646029327 2202 30-31 0.14663650967917619 2202 32-33 0.20266489119694597 2202 34-35 0.17484313053684986 2202 36-37 0.18826583442947253 2202 38-39 0.27198073240536047 2202 40-41 0.3664597252160675 2202 42-43 0.24130070224536126 2202 44-45 0.29309916278466375 2202 46-47 0.18895946964450872 2202 48-49 0.14122650824076288 2202 50-51 0.21752556534645606 2202 52-53 0.2747547092101854 2202 54-55 0.37179611881296637 2202 56-57 0.4399456753966646 2202 58-59 0.3937661871072393 2202 60-61 0.22732970003357167 2202 62-63 0.17246647180247265 2202 64-65 0.21122543320086606 2202 66-67 0.2208461528695409 2202 68-69 0.22406707019381145 2202 70-71 0.2726640728098815 2202 72-73 0.07212019758631527 2202 74-75 0.2085193390672586 2202 76-77 -0.009087110721871738 2202 78-79 0.37514220734407644 2202 80-81 0.44572268934513914 2202 82-83 0.4165307999124437 2202 84-85 0.3398945498453898 2202 86-87 0.4061551079260468 2202 88-89 0.3727468506326801 2202 90-91 0.23403433530624085 2202 92-93 0.243904155633885 2202 94-95 0.39328428593758247 2202 96-97 0.47084685630481715 2202 98-99 0.4007486787590757 2202 100-101 0.5207534967425609 1102 1 0.09371337751559139 1102 2 0.1475353034760829 1102 3 0.06907971483603603 1102 4 0.09982910347049767 1102 5 0.11678403922844893 1102 6 0.06884463179267186 1102 7 0.058721970595193795 1102 8 0.07628604408515116 1102 9 0.13756111846818442 1102 10-11 0.21253907865688149 1102 12-13 0.10490431499380293 1102 14-15 0.13432387711781502 1102 16-17 0.1772780756871768 1102 18-19 0.22396662930471223 1102 20-21 0.2199302247696977 1102 22-23 0.15125609616917046 1102 24-25 0.15981833218479125 1102 26-27 0.10474710135977006 1102 28-29 0.19670150109342188 1102 30-31 0.2610508299495038 1102 32-33 0.29599332215931184 1102 34-35 0.22667988081246193 1102 36-37 0.22629627283177456 1102 38-39 0.17647843282833975 1102 40-41 0.31074778179873874 1102 42-43 0.14040283184611013 1102 44-45 0.11939416186498875 1102 46-47 0.10101770194516746 1102 48-49 0.07407984238644616 1102 50-51 0.10789657097132732 1102 52-53 0.14635700558665832 1102 54-55 0.2514265553643469 1102 56-57 0.17453274447421308 1102 58-59 0.19573057383721704 1102 60-61 0.15294363716316894 1102 62-63 0.07556194502716096 1102 64-65 0.04282744808304528 1102 66-67 0.1110125738392469 1102 68-69 0.06781195846949117 1102 70-71 0.0821645052699509 1102 72-73 -0.0330248240328288 1102 74-75 -0.11605281852481397 1102 76-77 -0.2823585718662116 1102 78-79 0.02177326048364847 1102 80-81 0.22294374681509765 1102 82-83 0.1844265490786583 1102 84-85 0.16922480936384687 1102 86-87 0.24708264319669837 1102 88-89 0.12156463243449878 1102 90-91 0.08641724235566528 1102 92-93 -0.03861489439614019 1102 94-95 -0.015733673012839233 1102 96-97 0.19346750649494027 1102 98-99 0.1728758270329145 1102 100-101 0.2548766786461023 2305 1 0.08613742735029462 2305 2 0.14532399526255801 2305 3 0.05914893132175081 2305 4 0.09937246804075528 2305 5 0.046187932326475334 2305 6 0.02063053937588677 2305 7 0.0665393748544858 2305 8 -0.022195280575260767 2305 9 0.01679616920669247 2305 10-11 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-1.4870715539724912 1213 88-89 -1.4649925911709758 1213 90-91 -1.3978493625645854 1213 92-93 -1.4775995702419706 1213 94-95 -1.5079175383366952 1213 96-97 -1.6165627253149708 1213 98-99 -1.6669107420524227 1213 100-101 -1.545485237969043 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 4.0 9 17.0 10 37.0 11 29.0 12 68.0 13 204.0 14 553.0 15 1305.0 16 2603.0 17 4438.0 18 6666.0 19 9095.0 20 12363.0 21 15912.0 22 20355.0 23 26053.0 24 33360.0 25 42260.0 26 55400.0 27 76674.0 28 116660.0 29 150983.0 30 127635.0 31 129072.0 32 168900.0 33 236347.0 34 351728.0 35 571581.0 36 1021915.0 37 1708801.0 38 798840.0 39 4578.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.06285895803956 27.222142974109552 19.227583596449772 16.487414471401117 2 29.59083934182794 27.549478982824994 28.04896637736683 14.81071529798024 3 33.67494722393786 21.74842646465097 27.513147697636057 17.06347861377511 4 29.242474775708455 22.26415074457082 28.008293908989344 20.485080570731373 5 32.90115723448038 20.496825147626506 29.33696003012062 17.26505758777249 6 30.301901609956943 21.250279658853078 30.895551062282177 17.5522676689078 7 30.87653250417337 24.92967699358567 25.000184390452578 19.19360611178838 8 28.49979225342343 30.438982740702418 22.768638450361564 18.29258655551259 9 30.820337318625647 25.293646185980073 24.678168416268647 19.20784807912563 10-11 26.092943675916747 29.868399655944977 27.798318288828504 16.240338379309776 12-13 24.109130699113766 29.500768995992228 27.192973213511152 19.197127091382853 14-15 23.328816645294935 29.786863953914327 27.696771832444224 19.187547568346517 16-17 24.75148557241294 27.873681300946647 27.66896399609584 19.705869130544578 18-19 22.026870781629373 28.31968668374298 29.8340854707699 19.81935706385775 20-21 23.648263611754285 28.058227023400917 30.071438167263302 18.2220711975815 22-23 21.630741957166055 32.41363384323196 27.560173906706243 18.395450292895738 24-25 21.245906970977646 28.341673050088524 30.09051990732009 20.321900071613737 26-27 21.382584198826997 28.13814111243287 27.873584715471484 22.605689973268653 28-29 23.057516826067822 30.66177030990591 27.73955041744242 18.541162446583844 30-31 20.91955343090925 28.433912178866468 29.991941259172545 20.654593131051737 32-33 20.97703056912728 30.630959543329823 27.651271293146053 20.74073859439685 34-35 22.668681264068553 30.53430382418774 28.168864074031536 18.62815083771217 36-37 20.864410649058723 30.17788529081202 29.941196614217475 19.016507445911774 38-39 20.73573323997758 28.703249793457424 29.804578980221873 20.75643798634312 40-41 22.56002742475075 28.353593830414553 30.016895479930067 19.069483264904637 42-43 20.686887135189718 30.3037811851738 29.747779033904443 19.261552645732035 44-45 20.34722801496562 28.297840767703626 30.272154060535073 21.082777156795686 46-47 22.555041207367978 28.100465898773702 27.947472679155027 21.397020214703293 48-49 20.353949042773905 30.509595090921287 29.839245303960162 19.297210562344645 50-51 22.330377127230776 28.15344431538012 30.04221073511725 19.473967822271845 52-53 20.510769179970033 28.348161947896315 27.750465478047403 23.390603394086245 54-55 22.17958149376576 28.3357345640563 30.13505034504298 19.349633597134964 56-57 20.43103012153589 28.092175665981124 29.898737072275804 21.57805714020718 58-59 20.242791600173295 28.592971677884805 29.882071472000156 21.28216524994175 60-61 22.136332156529157 28.163926377350553 30.135669902256012 19.564071563864278 62-63 20.257906514925274 28.400968192173764 29.77854777320629 21.562577519694667 64-65 21.993993519323478 28.397876656392423 30.145657156405598 19.4624726678785 66-67 20.3294500792226 31.938986302043627 27.805955572106615 19.925608046627154 68-69 20.18848579000916 32.37016801290591 27.810283202082033 19.631062995002896 70-71 20.094667569115792 32.01521714728289 28.11298836378162 19.77712691981969 72-73 20.135005475473847 32.131018585694065 27.80413824262761 19.92983769620448 74-75 19.95114388486049 31.83999227015798 28.26234029751108 19.946523547470456 76-77 20.289433401863395 31.36788173602079 28.002675043669257 20.34000981844656 78-79 20.15389635661888 31.494141379654334 28.106027715605236 20.245934548121546 80-81 20.1459270698359 30.99019556616006 28.34182605988569 20.522051304118357 82-83 20.26969439523361 31.04329748442998 27.988004996454624 20.69900312388178 84-85 20.112451775552735 30.842772736784635 28.498326738023 20.54644874963963 86-87 20.3886566819131 30.43492123554606 28.293876885623998 20.882545196916844 88-89 20.30209213530864 30.857059404474636 28.223891866619148 20.616956593597575 90-91 20.368780281998635 30.352112311157004 28.421511808404137 20.857595598440223 92-93 20.39589967327542 30.484961814196392 28.089854179214303 21.02928433331389 94-95 20.154111932820417 30.40001033164402 28.5233146373918 20.922563098143765 96-97 20.37108626814087 30.191086730180416 28.222042852498618 21.215784149180095 98-99 20.29088963274626 30.522339480916617 28.14337784970995 21.043393036627172 100-101 20.407268232615838 30.142757498991422 28.251056164118733 21.19891810427401 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 23.0 1 17.0 2 20.5 3 37.5 4 36.0 5 27.5 6 32.5 7 40.0 8 44.0 9 50.0 10 57.0 11 66.5 12 88.0 13 109.5 14 144.0 15 208.5 16 316.5 17 508.5 18 845.5 19 1371.5 20 2268.0 21 3752.5 22 5950.0 23 8931.0 24 13076.0 25 18792.0 26 25822.0 27 34443.5 28 44642.0 29 56579.5 30 70404.5 31 86098.5 32 104631.0 33 126309.5 34 150787.0 35 178339.0 36 209562.0 37 244144.0 38 276435.0 39 301359.0 40 319069.0 41 324256.5 42 316358.5 43 302515.5 44 284651.0 45 266634.5 46 250924.5 47 233307.0 48 212919.5 49 192337.5 50 172775.5 51 153450.5 52 132788.5 53 112127.0 54 93544.5 55 76680.0 56 62469.0 57 50969.0 58 40964.0 59 32513.0 60 25184.5 61 19134.5 62 14419.0 63 10662.0 64 7780.0 65 5583.5 66 3937.5 67 2814.5 68 2020.0 69 1415.5 70 1031.5 71 754.5 72 559.5 73 384.5 74 278.0 75 210.5 76 155.5 77 123.0 78 98.0 79 66.5 80 50.5 81 43.5 82 29.0 83 22.0 84 16.5 85 13.5 86 11.5 87 9.0 88 8.0 89 4.5 90 1.5 91 0.5 92 0.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15908505808650478 2 0.0 3 0.0016682945709480023 4 0.010905378195354835 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.268298645098954E-5 22-23 9.65854751601475E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 6.673178283792009E-4 38-39 0.001606831086755181 40-41 0.0054702500931610816 42-43 0.0022039049331997293 44-45 9.307327606341486E-4 46-47 0.0011941476928890964 48-49 0.0030204912231900673 50-51 5.882933487027166E-4 52-53 0.014558065255956777 54-55 0.02676295711710269 56-57 0.028694666620305637 58-59 0.021099536073621313 60-61 0.030916132548989034 62-63 0.005628299052514049 64-65 0.010325865344393951 66-67 0.02841369069256703 68-69 0.027079055035808628 70-71 0.03127613295640413 72-73 0.03125857196092046 74-75 0.038862483005346615 76-77 0.018842948153970595 78-79 0.010641963263099888 80-81 0.014760016704018903 82-83 0.007033178691207104 84-85 0.01087903670212934 86-87 0.03440199015249617 88-89 0.01540099303917261 90-91 0.020783438154915377 92-93 0.04906542138135493 94-95 0.05593177061546723 96-97 0.04011809418242854 98-99 0.0357980892934474 100-101 0.035517113365708786 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5694438.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.53443711535482 #Duplication Level Percentage of deduplicated Percentage of total 1 96.77155494403674 84.70843590803887 2 2.165949585110994 3.7919035550585423 3 0.3578138473951581 0.939631011714439 4 0.21346733460575196 0.747429719089184 5 0.11852611166560283 0.5187558234060117 6 0.06436327690683795 0.3380401928963865 7 0.063095590299211 0.3866125886911733 8 0.0466161575992647 0.32644152927458436 9 0.023037056940944007 0.18148822309079202 >10 0.15535739003066448 2.5057870363453834 >50 0.013032259277334694 0.7978836351476593 >100 0.006785032287503573 1.058904664269825 >500 2.622613678803596E-4 0.15171966953404695 >1k 1.1915394178667903E-4 0.14629270357057417 >5k 0.0 0.0 >10k+ 1.9998534370165722E-5 3.4006737398725626 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 194262 3.411434104647377 TruSeq Adapter, Index 7 (97% over 35bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001053659729019791 0.0 0.0 2 0.001264391674823749 0.0 0.0 3 0.001633172579980676 0.0 0.0 4 0.0019492704986866132 0.0 0.0 5 0.00231805140384354 0.0 0.0 6 0.003073174209641057 0.0 0.0 7 0.003758053033503921 0.0 0.0 8 0.004460492852850448 0.0 0.0 9 0.004899517739942028 0.0 0.0 10-11 0.006690739279275672 0.0 0.0 12-13 0.008402936338932833 0.0 0.0 14-15 0.010404889824070434 0.0 3.5121990967326364E-5 16-17 0.012389282313724374 0.0 3.5121990967326364E-5 18-19 0.014654650731116926 0.0 3.5121990967326364E-5 20-21 0.017429288017535707 0.0 3.5121990967326364E-5 22-23 0.020642950191046068 0.0 5.2682986450989545E-5 24-25 0.024190271278746033 8.780497741831591E-6 5.2682986450989545E-5 26-27 0.028220519742246736 1.7560995483663182E-5 7.024398193465273E-5 28-29 0.03237369517413308 1.7560995483663182E-5 8.780497741831591E-5 30-31 0.04814346911846261 1.7560995483663182E-5 1.0536597290197909E-4 32-33 0.053394206768077906 1.7560995483663182E-5 1.4048796386930545E-4 34-35 0.05851323695156572 1.7560995483663182E-5 1.4926846161113705E-4 36-37 0.06421177998601443 1.7560995483663182E-5 1.5804895935296864E-4 38-39 0.0699893475001396 1.7560995483663182E-5 1.7560995483663182E-4 40-41 0.07634642786522568 1.7560995483663182E-5 2.1073194580395818E-4 42-43 0.08369570447513872 1.7560995483663182E-5 2.4585393677128455E-4 44-45 0.09164205493149631 1.7560995483663182E-5 2.4585393677128455E-4 46-47 0.09929864896237346 1.7560995483663182E-5 2.5463443451311614E-4 48-49 0.10754353634195332 1.7560995483663182E-5 2.721954299967793E-4 50-51 0.1168508639482948 1.7560995483663182E-5 3.2487841644776886E-4 52-53 0.12712404630623778 2.6341493225494773E-5 3.5121990967326364E-4 54-55 0.1383630834157822 4.3902488709157955E-5 3.600004074150952E-4 56-57 0.1500411454124182 5.2682986450989545E-5 3.687809051569268E-4 58-59 0.16238652523743344 5.2682986450989545E-5 3.951223983824216E-4 60-61 0.17656702909049146 5.2682986450989545E-5 4.4780538483341114E-4 62-63 0.191555338735798 5.2682986450989545E-5 4.917078735425691E-4 64-65 0.207404137159804 5.2682986450989545E-5 5.092688690262323E-4 66-67 0.22572025544926472 5.2682986450989545E-5 5.092688690262323E-4 68-69 0.24566076582096424 7.024398193465273E-5 5.531713577353902E-4 70-71 0.26830566949714796 7.024398193465273E-5 5.707323532190534E-4 72-73 0.29247837978041025 7.024398193465273E-5 5.882933487027166E-4 74-75 0.31946962983880056 7.024398193465273E-5 6.23415339670043E-4 76-77 0.34908624872199856 8.780497741831591E-5 6.497568328955377E-4 78-79 0.38275067706418087 8.780497741831591E-5 6.497568328955377E-4 80-81 0.41977803604148467 8.780497741831591E-5 6.497568328955377E-4 82-83 0.4616346687767959 8.780497741831591E-5 6.673178283792009E-4 84-85 0.5087683806549479 8.780497741831591E-5 6.673178283792009E-4 86-87 0.5624962463372154 8.780497741831591E-5 6.760983261210325E-4 88-89 0.6225109484026343 8.780497741831591E-5 6.848788238628641E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 27785 0.0 77.94712 3 CGGAAGA 27905 0.0 77.1517 4 GATCGGA 28160 0.0 76.92411 1 ATCGGAA 28250 0.0 76.56322 2 GAAGAGC 30300 0.0 70.88795 6 GAGCACA 31560 0.0 67.93744 9 AGAGCAC 31575 0.0 67.90517 8 AAGAGCA 34690 0.0 61.889736 7 GGAAGAG 37205 0.0 58.24218 5 GCCGTCT 23605 0.0 45.00001 50-51 ATGCCGT 23630 0.0 44.928547 48-49 TGCCGTC 23535 0.0 44.911816 50-51 TATGCCG 23650 0.0 44.880512 48-49 TCTCGTA 23695 0.0 44.82592 42-43 CGTATGC 23785 0.0 44.750088 46-47 CTCGTAT 23695 0.0 44.716274 44-45 CGTCTTC 23825 0.0 44.61478 52-53 TATCTCG 23705 0.0 44.43774 40-41 ATCTCGT 23750 0.0 44.40217 42-43 TCGTATC 23505 0.0 44.361618 38-39 >>END_MODULE