FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_35008-2.341000000358b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_35008-2.341000000358b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5694438
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT1942623.411434104647377TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG277850.077.947123
CGGAAGA279050.077.15174
GATCGGA281600.076.924111
ATCGGAA282500.076.563222
GAAGAGC303000.070.887956
GAGCACA315600.067.937449
AGAGCAC315750.067.905178
AAGAGCA346900.061.8897367
GGAAGAG372050.058.242185
GCCGTCT236050.045.0000150-51
ATGCCGT236300.044.92854748-49
TGCCGTC235350.044.91181650-51
TATGCCG236500.044.88051248-49
TCTCGTA236950.044.8259242-43
CGTATGC237850.044.75008846-47
CTCGTAT236950.044.71627444-45
CGTCTTC238250.044.6147852-53
TATCTCG237050.044.4377440-41
ATCTCGT237500.044.4021742-43
TCGTATC235050.044.36161838-39
Produced by FastQC (version 0.11.2)