##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_35008-1.341000000357b4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2356005 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67109534996742 33.0 33.0 33.0 33.0 33.0 2 32.66020827629823 33.0 33.0 33.0 33.0 33.0 3 32.72711815127727 33.0 33.0 33.0 33.0 33.0 4 36.67882283781231 37.0 37.0 37.0 37.0 37.0 5 36.68685889885633 37.0 37.0 37.0 37.0 37.0 6 36.559918166557374 37.0 37.0 37.0 37.0 37.0 7 36.51001037773689 37.0 37.0 37.0 37.0 37.0 8 36.4488534616862 37.0 37.0 37.0 37.0 37.0 9 36.459737564224184 37.0 37.0 37.0 37.0 37.0 10-11 36.341794690588515 37.0 37.0 37.0 37.0 37.0 12-13 36.36916729803205 37.0 37.0 37.0 37.0 37.0 14-15 38.25397654079681 40.0 37.0 40.0 37.0 40.0 16-17 38.211996578954626 40.0 37.0 40.0 37.0 40.0 18-19 38.153610879433614 40.0 37.0 40.0 37.0 40.0 20-21 38.15562785308181 40.0 37.0 40.0 37.0 40.0 22-23 38.09096903444602 40.0 37.0 40.0 37.0 40.0 24-25 38.06716411892165 40.0 37.0 40.0 37.0 40.0 26-27 37.97492683589381 40.0 37.0 40.0 35.0 40.0 28-29 37.87577785276347 40.0 37.0 40.0 33.0 40.0 30-31 37.77538884679787 40.0 37.0 40.0 33.0 40.0 32-33 37.68955902046048 40.0 37.0 40.0 33.0 40.0 34-35 37.62420410822558 40.0 37.0 40.0 33.0 40.0 36-37 37.568033811473235 40.0 37.0 40.0 33.0 40.0 38-39 37.447495230273276 40.0 37.0 40.0 33.0 40.0 40-41 37.30364154575224 40.0 37.0 40.0 33.0 40.0 42-43 37.263277454844115 40.0 37.0 40.0 33.0 40.0 44-45 37.157024496976874 40.0 37.0 40.0 33.0 40.0 46-47 37.090626081014264 38.5 37.0 40.0 33.0 40.0 48-49 36.92554536174583 37.0 37.0 40.0 33.0 40.0 50-51 36.754263467182795 37.0 37.0 40.0 33.0 40.0 52-53 36.59000278012992 37.0 37.0 40.0 33.0 40.0 54-55 36.27435722759502 37.0 37.0 40.0 33.0 40.0 56-57 36.1540238666726 37.0 37.0 40.0 33.0 40.0 58-59 35.969550998406206 37.0 37.0 40.0 33.0 40.0 60-61 35.690118866470996 37.0 35.0 40.0 27.0 40.0 62-63 35.454648865346215 37.0 33.0 40.0 27.0 40.0 64-65 35.20981937644444 37.0 33.0 37.0 27.0 40.0 66-67 34.75028087801172 37.0 33.0 37.0 27.0 40.0 68-69 34.503821086967136 37.0 33.0 37.0 27.0 40.0 70-71 34.21528880456536 37.0 33.0 37.0 27.0 40.0 72-73 33.83633353919028 37.0 33.0 37.0 27.0 37.0 74-75 33.41339640620457 37.0 33.0 37.0 27.0 37.0 76-77 30.76667706562592 33.0 30.0 35.0 24.5 37.0 78-79 33.16192325568069 35.0 33.0 37.0 27.0 37.0 80-81 33.536941347747565 37.0 33.0 37.0 27.0 37.0 82-83 33.458692787154526 37.0 33.0 37.0 27.0 37.0 84-85 33.35144195364611 37.0 33.0 37.0 27.0 37.0 86-87 33.24231548744591 37.0 33.0 37.0 27.0 37.0 88-89 33.087420654879764 37.0 33.0 37.0 27.0 37.0 90-91 32.980481365701685 37.0 33.0 37.0 27.0 37.0 92-93 32.79881536753955 33.0 33.0 37.0 27.0 37.0 94-95 32.67789435930739 33.0 33.0 37.0 27.0 37.0 96-97 32.52411985543324 33.0 33.0 37.0 27.0 37.0 98-99 32.395221147663094 33.0 33.0 37.0 24.5 37.0 100-101 30.811604814081463 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.12476767706885283 2306 2 0.18085481735132447 2306 3 0.15497086540452187 2306 4 0.16691473066430973 2306 5 0.1685095578730369 2306 6 0.15188210821953163 2306 7 0.20671700398707316 2306 8 0.13323728515192812 2306 9 0.11356267298300793 2306 10-11 0.22146222984527952 2306 12-13 0.26235441628747935 2306 14-15 0.3962750165978619 2306 16-17 0.3982221762218785 2306 18-19 0.5519590500560341 2306 20-21 0.5231306708691292 2306 22-23 0.40177148672165686 2306 24-25 0.326040710256855 2306 26-27 0.5190801295596046 2306 28-29 0.5613183396553723 2306 30-31 0.45090591904615707 2306 32-33 0.49994002254314296 2306 34-35 0.43911299467066556 2306 36-37 0.48247089531957954 2306 38-39 0.3999326014388771 2306 40-41 0.45076282183484295 2306 42-43 0.4209008154159548 2306 44-45 0.3775014583998768 2306 46-47 0.4190383040720249 2306 48-49 0.3804752172786223 2306 50-51 0.4693309306054516 2306 52-53 0.38310898471100074 2306 54-55 0.6082159705700931 2306 56-57 0.5998536258306686 2306 58-59 0.5553269748212912 2306 60-61 0.44158993542710334 2306 62-63 0.262959965361091 2306 64-65 0.36252093537559205 2306 66-67 0.574717293524138 2306 68-69 0.6067266465210892 2306 70-71 0.31645423241555903 2306 72-73 0.2823649808368671 2306 74-75 0.3377835954479167 2306 76-77 0.09383759790018686 2306 78-79 0.5552529824212584 2306 80-81 0.5430818385434364 2306 82-83 0.5658692290101044 2306 84-85 0.5467132916184454 2306 86-87 0.6810188472678789 2306 88-89 0.6470830805384225 2306 90-91 0.6432445762511563 2306 92-93 0.39849632244094124 2306 94-95 0.42842440522744596 2306 96-97 0.4864064963680832 2306 98-99 0.6485417804792704 2306 100-101 0.4413531449347943 2307 1 0.08062763284003438 2307 2 0.14441254368582435 2307 3 0.1113115166623615 2307 4 0.13244475782722986 2307 5 0.12626391281713012 2307 6 0.08931198163236331 2307 7 0.17334006988220807 2307 8 0.26848120187807467 2307 9 0.18937940239372608 2307 10-11 0.15125940171324004 2307 12-13 0.21798338459176136 2307 14-15 0.30634616073765386 2307 16-17 0.3559473935312667 2307 18-19 0.40869382934071297 2307 20-21 0.3971069283769779 2307 22-23 0.45410660394251323 2307 24-25 0.4344253137495073 2307 26-27 0.31949669205545916 2307 28-29 0.380993368974174 2307 30-31 0.43465361068121666 2307 32-33 0.41783107375653117 2307 34-35 0.39257822939620723 2307 36-37 0.48007632524613086 2307 38-39 0.4239614878558484 2307 40-41 0.47252621940987183 2307 42-43 0.47977635031045907 2307 44-45 0.519044841167549 2307 46-47 0.4246751531251931 2307 48-49 0.48298473758558913 2307 50-51 0.5074921737467832 2307 52-53 0.6369287323579513 2307 54-55 0.5713835847382924 2307 56-57 0.5184332612509266 2307 58-59 0.48560846550872583 2307 60-61 0.4482085567006564 2307 62-63 0.3847972487310827 2307 64-65 0.49406950554806883 2307 66-67 0.43865329580442847 2307 68-69 0.4014222684480515 2307 70-71 0.4427566695899898 2307 72-73 0.318090740438393 2307 74-75 0.3822717815712835 2307 76-77 0.17874843975740973 2307 78-79 0.34513455378823465 2307 80-81 0.3844121693416511 2307 82-83 0.4428111844261977 2307 84-85 0.4699371338730316 2307 86-87 0.4342857347162905 2307 88-89 0.46427839146264915 2307 90-91 0.27498956125659646 2307 92-93 0.3078677104310472 2307 94-95 0.38686930701700817 2307 96-97 0.5102759561412356 2307 98-99 0.4461905696218338 2307 100-101 0.17136925668803826 2201 1 0.14485544064995537 2201 2 0.21922378547377974 2201 3 0.08542409208455837 2201 4 0.15811767359613071 2201 5 0.07259173733530133 2201 6 0.09854894354437249 2201 7 0.09537960982486737 2201 8 0.18284176256648976 2201 9 0.10551681559192616 2201 10-11 0.2074008815841708 2201 12-13 0.16359422636303833 2201 14-15 0.22889648785677963 2201 16-17 0.22984319785344098 2201 18-19 0.27623613895460863 2201 20-21 0.3958516293688703 2201 22-23 0.3242472775655898 2201 24-25 0.2970036660220359 2201 26-27 0.18522548914075543 2201 28-29 0.18508100195532506 2201 30-31 0.31852789052990005 2201 32-33 0.3528940513077927 2201 34-35 0.37413810365643485 2201 36-37 0.31736062737157056 2201 38-39 0.2968125497834748 2201 40-41 0.5125821279827818 2201 42-43 0.3731925925249726 2201 44-45 0.3412877692591252 2201 46-47 0.34049366504460465 2201 48-49 0.24537139475608427 2201 50-51 0.31321737842280584 2201 52-53 0.34895297321617846 2201 54-55 0.5527864614458835 2201 56-57 0.5869218081976939 2201 58-59 0.37333095707079167 2201 60-61 0.29100099258037204 2201 62-63 0.3925236543293522 2201 64-65 0.27525484797308764 2201 66-67 0.590564645609966 2201 68-69 0.4285129320816097 2201 70-71 0.2542903748657821 2201 72-73 0.2755420868695708 2201 74-75 0.26967223735479706 2201 76-77 0.1535289361913037 2201 78-79 0.26817468056376015 2201 80-81 0.3726072344397622 2201 82-83 0.5039051739002716 2201 84-85 0.5482233093390505 2201 86-87 0.47862486905468415 2201 88-89 0.48437748526514923 2201 90-91 0.5092958705684865 2201 92-93 0.3358607011444761 2201 94-95 0.40103997153367743 2201 96-97 0.5373562622973651 2201 98-99 0.23282980532722064 2201 100-101 0.4458088830883753 1101 1 0.10618768305479165 1101 2 0.14348433462778587 1101 3 0.13883348691861386 1101 4 0.13688415411714772 1101 5 0.12462031755057978 1101 6 0.17864015004877842 1101 7 0.17553638090084434 1101 8 0.17925723610228061 1101 9 0.12789412757809515 1101 10-11 0.16623820648037224 1101 12-13 0.10371843287708771 1101 14-15 0.28362595730084195 1101 16-17 0.3807989437469317 1101 18-19 0.3590273687406196 1101 20-21 0.355017766776875 1101 22-23 0.3542717690257433 1101 24-25 0.39696056659423107 1101 26-27 0.4164497132953855 1101 28-29 0.36749561894565375 1101 30-31 0.4395237198809028 1101 32-33 0.42764496160383203 1101 34-35 0.39715794844406105 1101 36-37 0.4579571709558081 1101 38-39 0.3862870361345401 1101 40-41 0.5565716257754758 1101 42-43 0.5926562644329252 1101 44-45 0.5011366689000525 1101 46-47 0.5564517353877179 1101 48-49 0.5071151847396465 1101 50-51 0.34823974583825645 1101 52-53 0.34250612022902516 1101 54-55 0.5882107520557724 1101 56-57 0.5057601672822258 1101 58-59 0.5730804756660177 1101 60-61 0.6455636805991603 1101 62-63 0.4930956248069833 1101 64-65 0.37525380883648296 1101 66-67 0.6151300823924757 1101 68-69 0.6556099283087988 1101 70-71 0.5008396372396149 1101 72-73 0.28409690268632204 1101 74-75 0.32844864905280957 1101 76-77 0.1078241207580426 1101 78-79 0.5156949171306309 1101 80-81 0.4891527138615075 1101 82-83 0.5175387698795078 1101 84-85 0.50880848674813 1101 86-87 0.4051824049352035 1101 88-89 0.46119570835639223 1101 90-91 0.5397908187563942 1101 92-93 0.46014440819998725 1101 94-95 0.2660981866879055 1101 96-97 0.4833580233864936 1101 98-99 0.4559135075334382 1101 100-101 0.4151084580072819 2304 1 0.0994227945073547 2304 2 0.15661748662515151 2304 3 0.08399041279697173 2304 4 0.10668249319844136 2304 5 0.07844416057972836 2304 6 0.10666041836361018 2304 7 0.1340620259163856 2304 8 0.06144913268353491 2304 9 0.17344490717584904 2304 10-11 0.1360923138671879 2304 12-13 0.10772159770003498 2304 14-15 0.06489925561788823 2304 16-17 0.13446409130465042 2304 18-19 0.3396947347637891 2304 20-21 0.3258365181937961 2304 22-23 0.23510182857402384 2304 24-25 0.2665208851845904 2304 26-27 0.2727190022006525 2304 28-29 0.3793242923505531 2304 30-31 0.41561445025715926 2304 32-33 0.32772996567684487 2304 34-35 0.30934149454502347 2304 36-37 0.41890095695816854 2304 38-39 0.3570635353933156 2304 40-41 0.4725282214509505 2304 42-43 0.39368608128118154 2304 44-45 0.3457192607382993 2304 46-47 0.1823439714855155 2304 48-49 0.48478094381783876 2304 50-51 0.3766793100322303 2304 52-53 0.3292404191226055 2304 54-55 0.5795431534185624 2304 56-57 0.5857619028009822 2304 58-59 0.35483501697795106 2304 60-61 0.43704042078609007 2304 62-63 0.22717780570891932 2304 64-65 0.2881008955501798 2304 66-67 0.40385657054302015 2304 68-69 0.31042274443331763 2304 70-71 0.2880780997880734 2304 72-73 0.24645295571686887 2304 74-75 0.3924754341951271 2304 76-77 0.31304285291161804 2304 78-79 0.527734964907367 2304 80-81 0.5906495533546519 2304 82-83 0.4903899409684911 2304 84-85 0.5624663424011231 2304 86-87 0.5223858027356414 2304 88-89 0.44488149132295973 2304 90-91 0.30989364597095914 2304 92-93 0.33041619861474913 2304 94-95 0.5687741339881072 2304 96-97 0.4116063624905095 2304 98-99 0.4337435048392848 2304 100-101 0.37822902522205837 2202 1 0.13682779080583884 2202 2 0.08174597893258806 2202 3 0.0816954754893402 2202 4 0.08237978801908241 2202 5 0.12078097057656834 2202 6 0.11937134620216483 2202 7 0.09486163731067876 2202 8 0.06548494719643116 2202 9 -0.02849621227161947 2202 10-11 0.14569238089742953 2202 12-13 0.14847333338026658 2202 14-15 0.17352749019828906 2202 16-17 0.20344924071208936 2202 18-19 0.2135143343217294 2202 20-21 0.21969603892756595 2202 22-23 0.14729878718284795 2202 24-25 0.111711359253853 2202 26-27 0.08262696289038018 2202 28-29 0.1374534061725754 2202 30-31 0.24414939401327018 2202 32-33 0.19067624178629217 2202 34-35 0.12441343538871763 2202 36-37 0.1842878975154889 2202 38-39 0.2574425956547728 2202 40-41 0.30868000676750285 2202 42-43 0.10143295302645328 2202 44-45 0.19102884075989124 2202 46-47 0.17343563109916005 2202 48-49 0.14428566244991714 2202 50-51 0.23384698533434545 2202 52-53 0.14023072731323083 2202 54-55 0.2785430028126399 2202 56-57 0.3428644569501742 2202 58-59 0.42541330130072197 2202 60-61 0.2641197901078769 2202 62-63 0.24539497096604634 2202 64-65 0.12870478287997145 2202 66-67 0.2915208847875874 2202 68-69 0.16488121007713374 2202 70-71 0.11846305701386939 2202 72-73 0.2010176939913535 2202 74-75 0.3396363004125291 2202 76-77 0.043104219522497544 2202 78-79 0.344974040144578 2202 80-81 0.4241711440925968 2202 82-83 0.347812585735781 2202 84-85 0.48468145856919875 2202 86-87 0.5694942750612739 2202 88-89 0.5780851126529996 2202 90-91 0.34595952227471116 2202 92-93 0.27501337019307215 2202 94-95 0.29585042755510926 2202 96-97 0.4948386201581556 2202 98-99 0.45513092479011874 2202 100-101 0.46217157012277355 1102 1 0.10710551841606275 1102 2 0.15559262673625085 1102 3 0.0821058461272699 1102 4 0.0896129467693072 1102 5 0.04222057886826036 1102 6 0.059443186147582594 1102 7 0.024807657016495455 1102 8 0.07041541495875236 1102 9 0.1050247477348023 1102 10-11 0.20847056001568376 1102 12-13 0.13574381679487857 1102 14-15 0.1365514903588192 1102 16-17 0.21766773046786625 1102 18-19 0.1928463030974399 1102 20-21 0.21453781044443332 1102 22-23 0.10990438989591667 1102 24-25 0.08710065951672874 1102 26-27 0.03775559251332794 1102 28-29 0.04532820801070869 1102 30-31 0.18928163570050316 1102 32-33 0.16539129004748077 1102 34-35 0.027932188022511184 1102 36-37 0.10339270758110786 1102 38-39 0.1597974609047057 1102 40-41 0.22615186276654242 1102 42-43 0.06555562274330384 1102 44-45 0.10422692235241726 1102 46-47 0.00794539370625813 1102 48-49 0.12244019860065691 1102 50-51 0.06898334076146995 1102 52-53 0.053140847113361644 1102 54-55 0.270353148699904 1102 56-57 0.16342111619204758 1102 58-59 0.0520663223911626 1102 60-61 0.06335874822799781 1102 62-63 0.027855388761480526 1102 64-65 0.14188079074624937 1102 66-67 0.2117820571031146 1102 68-69 0.26727370099680314 1102 70-71 2.783199922120616E-4 1102 72-73 0.00904350018403477 1102 74-75 0.061120557832303746 1102 76-77 -0.19544804504114666 1102 78-79 0.19532170133294358 1102 80-81 0.276385540175518 1102 82-83 0.24536792055407375 1102 84-85 0.2808165497738244 1102 86-87 0.33822684183429885 1102 88-89 0.21533853909732414 1102 90-91 0.18154588071188726 1102 92-93 0.1556908329185802 1102 94-95 -0.07750909324435895 1102 96-97 0.07037541304639916 1102 98-99 0.29331245108795656 1102 100-101 0.21836826709064638 2305 1 0.0666843066262004 2305 2 0.0863775489302725 2305 3 0.04489566253759847 2305 4 0.03929395547558556 2305 5 0.06172995065148257 2305 6 -0.019631211219042655 2305 7 0.08948747798923051 2305 8 0.09740411452249731 2305 9 -8.745173786337546E-4 2305 10-11 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-1.5499409500927754 1213 90-91 -1.4904118277472378 1213 92-93 -1.7197615491396832 1213 94-95 -1.596369183351925 1213 96-97 -1.7716920200901214 1213 98-99 -1.6102729994635396 1213 100-101 -1.7875088062986606 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 11.0 9 22.0 10 24.0 11 10.0 12 22.0 13 50.0 14 171.0 15 417.0 16 805.0 17 1327.0 18 2007.0 19 2838.0 20 4011.0 21 5094.0 22 6677.0 23 8566.0 24 10978.0 25 13977.0 26 17744.0 27 23725.0 28 33504.0 29 44109.0 30 45547.0 31 52783.0 32 70388.0 33 99278.0 34 152912.0 35 267095.0 36 534396.0 37 789911.0 38 167543.0 39 63.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.611671349371434 25.531942933494538 24.771742055450197 15.084643661683828 2 28.821373469071588 23.463702326607965 33.899843166716536 13.81508103760391 3 36.85976708423194 19.393164312282174 26.840959456425175 16.906109147060707 4 27.930453076329442 19.208264671898096 34.77019325381766 18.091088997954802 5 32.296281204836156 18.108026086532075 32.73656040628097 16.8591323023508 6 25.67876553742458 19.3826838228272 38.19015664228217 16.74839399746605 7 31.475102981530174 20.82279112310882 29.72841738451319 17.97368851084781 8 27.526214927387677 28.13198613755064 26.6491794372253 17.69261949783638 9 30.347813353537024 24.53084776984769 25.93818773729258 19.18315113932271 10-11 24.26902744264125 28.82279536758199 28.954649926464505 17.95352726331226 12-13 23.68040390406642 26.875834304256568 28.99159806536913 20.45216372630788 14-15 22.96291816019066 27.410595478362737 29.814495300307087 19.811991061139516 16-17 22.68719718336761 27.65314589739835 30.57907347395273 19.080583445281313 18-19 20.93253197679971 27.961337093936557 31.387963947444934 19.7181669818188 20-21 21.824246016612452 28.10931732486815 30.972386076676035 19.094050581843362 22-23 21.14855116907746 29.48491576623629 30.28045804695495 19.086075017731304 24-25 21.522704748079907 27.39327802784799 31.149700446306355 19.93431677776575 26-27 21.40250126803636 27.55601112900864 30.257554631675234 20.78393297127977 28-29 21.350867251979516 28.769272560966552 30.051464237130226 19.828395949923706 30-31 20.20933741651652 28.72555448736314 30.44424778385445 20.62086031226589 32-33 19.96341264131443 29.515259942147832 30.418653610667214 20.10267380587053 34-35 21.051780450381045 28.931984439761376 30.632108166154147 19.384126943703432 36-37 20.175688612816757 29.36048232874 31.26180901291243 19.202020045530823 38-39 20.81103800836047 29.742354365094442 29.71847883365164 19.728128792893454 40-41 20.643973319455963 28.590238696976154 31.049771761987273 19.71601622158061 42-43 20.620754904382682 29.446033326626917 30.771313871153684 19.161897897836717 44-45 20.15626604957084 28.873798696254894 31.166091251970517 19.803844002203753 46-47 21.096637273315718 28.79780133490381 30.256666560553487 19.84889483122699 48-49 20.684893160003305 30.11480182238051 30.0449567782058 19.15534823941039 50-51 21.35138066133968 29.357100548580313 29.649015987118794 19.642502802961218 52-53 20.22776245767633 28.983497590480106 29.38455360205395 21.404186349789615 54-55 21.439852626314387 29.134982017710147 29.729643670025936 19.69552168594953 56-57 20.732503641735583 29.00882805106539 29.40747656488488 20.851191742314143 58-59 20.577809136994635 28.778506143703613 30.21907016808738 20.424614551214372 60-61 20.80150775744432 29.18131810744003 30.337292371287393 19.67988176382826 62-63 20.237279394364506 29.23676896776035 29.955250124953707 20.57070151292143 64-65 21.156283720626178 28.99772217302825 29.801753493785853 20.044240612559715 66-67 20.841741564136846 30.18221218164241 28.853906308709902 20.122139945510845 68-69 21.051872954143086 30.263865125224488 28.73045423861219 19.953807682020237 70-71 20.526519698846528 31.14104388973274 28.782892886294714 19.54954352512602 72-73 20.249012530660057 31.475678087754954 28.61669800782333 19.658611373761655 74-75 20.949385604996177 30.518907842235578 28.50175853824136 20.029948014526887 76-77 21.309160331268988 29.61451008427763 29.115687729564417 19.96064185488897 78-79 21.13481993261614 29.43794712272955 29.6655385084285 19.761694436225806 80-81 20.35666725107943 30.052584413329537 29.4384628217967 20.152285513794336 82-83 19.564589443577688 29.89720675655513 29.677115922924486 20.861087876942694 84-85 19.70162273678044 29.45239133733844 30.061859477425905 20.78412644845521 86-87 19.668314007734768 29.656661116558848 30.722511097208127 19.95251377849826 88-89 19.947237257653388 30.12483446639437 30.27727714703375 19.650651128918494 90-91 20.398953338129225 29.596875552565848 29.647162361437978 20.35700874786695 92-93 19.678502702818623 30.40964491891544 29.594499707307005 20.31735267095893 94-95 19.32252084673746 30.87425706623179 29.58317142207981 20.22005066495094 96-97 19.864882021763417 31.380949813019072 28.98864696463307 19.765521200584445 98-99 20.315679503017506 30.441494013073772 28.678184430998392 20.564642052910326 100-101 20.242453681186273 30.280894618502302 28.34591875793334 21.13073294237808 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 146.0 1 94.0 2 66.5 3 152.5 4 160.5 5 87.5 6 67.0 7 78.0 8 79.5 9 72.5 10 81.0 11 79.5 12 74.0 13 79.5 14 89.5 15 101.0 16 113.0 17 131.5 18 167.0 19 225.5 20 319.0 21 473.5 22 701.0 23 1055.0 24 1516.0 25 2193.0 26 3336.0 27 4987.0 28 6757.0 29 8480.0 30 10602.0 31 13059.0 32 18571.5 33 29115.5 34 44766.0 35 67575.5 36 100580.0 37 142066.0 38 182527.5 39 200129.0 40 197049.5 41 178060.0 42 152493.0 43 143092.5 44 135002.5 45 124997.0 46 122138.5 47 108393.5 48 81196.5 49 61241.5 50 48809.0 51 39908.0 52 33024.5 53 25624.0 54 18956.5 55 13345.5 56 9292.0 57 6193.5 58 4438.0 59 3319.0 60 2390.5 61 1740.0 62 1210.5 63 867.0 64 635.0 65 467.0 66 330.5 67 224.0 68 160.5 69 120.0 70 98.5 71 79.0 72 58.0 73 42.5 74 31.5 75 25.5 76 21.0 77 17.0 78 11.0 79 5.5 80 4.5 81 2.5 82 2.0 83 1.0 84 0.0 85 1.0 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15840373853196407 2 0.0 3 0.001952457656074584 4 0.010314069791872259 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 6.366709748069296E-5 22-23 8.488946330759061E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.852275355952131E-4 38-39 0.0015492327053635286 40-41 0.005560259846647185 42-43 0.00216468131434356 44-45 9.76228828037292E-4 46-47 0.001273341949613859 48-49 0.0032045772398615452 50-51 6.154486089800319E-4 52-53 0.01481321134717456 54-55 0.02682507040519863 56-57 0.02852285967135044 58-59 0.02139214475351283 60-61 0.030517762059078826 62-63 0.005623926944127877 64-65 0.010653627645102621 66-67 0.028607749134658033 68-69 0.026952404600160015 70-71 0.030878542278136082 72-73 0.031493990887116116 74-75 0.03921893204810686 76-77 0.019397242365784453 78-79 0.010526293450141236 80-81 0.015449882321981488 82-83 0.006769934698780351 84-85 0.011141742059121267 86-87 0.033764784030594165 88-89 0.015513549419462183 90-91 0.021922703899185273 92-93 0.0487477743043839 94-95 0.0560270457830098 96-97 0.04040738453441312 98-99 0.03529279437013079 100-101 0.03537768383343838 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2356005.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.585559315484176 #Duplication Level Percentage of deduplicated Percentage of total 1 87.45541105885181 34.619713619302296 2 5.264607320818573 4.168048507419917 3 1.9285184994562679 2.290244503537039 4 1.1302002366243091 1.789584340210634 5 0.6757223769438379 1.3374424116655124 6 0.5471982402926884 1.299668903906087 7 0.381893453779881 1.0582226176759015 8 0.28012796444529725 0.8871217721980126 9 0.21224430138693087 0.7561628443733268 >10 1.6506475995318945 12.962376540644001 >50 0.22174003804709663 6.166552443407526 >100 0.21234090497259384 18.153198768343024 >500 0.03197243646863246 8.525360822939916 >1k 0.007267654767632586 4.603727129841289 >5k 0.0 0.0 >10k+ 1.0791361267232881E-4 1.382574774535539 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 32365 1.373723739975085 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 4984 0.2115445425625158 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3865 0.16404888784191884 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 3818 0.16205398545419045 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3783 0.16056841984630763 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3195 0.13561091763387598 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 3051 0.12949887627572945 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3005 0.12754641861965488 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 2681 0.11379432556382521 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.48894633075906E-4 0.0 0.0 2 8.913393647297013E-4 0.0 0.0 3 9.337840963834967E-4 0.0 0.0 4 0.0010611182913448825 0.0 0.0 5 0.0011884524863062685 0.0 0.0 6 0.001273341949613859 0.0 0.0 7 0.0014431208762290403 0.0 0.0 8 0.0017826787294594026 0.0 0.0 9 0.001994902387728379 0.0 4.2444731653795305E-5 10-11 0.003331911434822931 0.0 4.2444731653795305E-5 12-13 0.004393029726167814 0.0 4.2444731653795305E-5 14-15 0.005645149309954776 0.0 4.2444731653795305E-5 16-17 0.007173159649491406 0.0 8.488946330759061E-5 18-19 0.009592509353757737 0.0 1.273341949613859E-4 20-21 0.012393861642908227 0.0 1.6977892661518122E-4 22-23 0.014707099518040072 0.0 1.6977892661518122E-4 24-25 0.017317450514748485 0.0 1.6977892661518122E-4 26-27 0.020819140876186595 0.0 1.6977892661518122E-4 28-29 0.024426943066759195 0.0 1.6977892661518122E-4 30-31 0.03809414665928128 0.0 1.6977892661518122E-4 32-33 0.04267817767789118 0.0 1.6977892661518122E-4 34-35 0.045925199649406515 0.0 1.9100129244207885E-4 36-37 0.050339451741401225 0.0 2.122236582689765E-4 38-39 0.055050816954972506 0.0 2.3344602409587417E-4 40-41 0.060632299167446585 0.0 2.546683899227718E-4 42-43 0.06725367730543866 0.0 2.546683899227718E-4 44-45 0.07412972383335349 0.0 2.546683899227718E-4 46-47 0.08056010067890348 0.0 2.546683899227718E-4 48-49 0.08735125774351073 0.0 2.546683899227718E-4 50-51 0.0940363029789835 0.0 2.546683899227718E-4 52-53 0.10174002177414734 0.0 2.546683899227718E-4 54-55 0.10991063261750295 0.0 2.546683899227718E-4 56-57 0.11954558670291446 0.0 2.7589075574966943E-4 58-59 0.1290319842275377 0.0 2.971131215765671E-4 60-61 0.14034350521327416 0.0 3.3955785323036244E-4 62-63 0.1528859234169707 0.0 3.6078021905726007E-4 64-65 0.165258562694052 0.0 3.820025848841577E-4 66-67 0.17915921231066997 0.0 3.820025848841577E-4 68-69 0.19363286580461417 0.0 3.820025848841577E-4 70-71 0.21010142168628676 0.0 4.0322495071105533E-4 72-73 0.2277584300542656 0.0 4.24447316537953E-4 74-75 0.24774989866320318 0.0 4.24447316537953E-4 76-77 0.2714552812918478 0.0 4.24447316537953E-4 78-79 0.29456643767733937 0.0 4.24447316537953E-4 80-81 0.320160610864578 0.0 4.24447316537953E-4 82-83 0.3478557982686794 0.0 4.24447316537953E-4 84-85 0.3807716876661976 0.0 4.456696823648507E-4 86-87 0.41837771991146033 0.0 4.6689204819174834E-4 88-89 0.45846676895846994 0.0 4.6689204819174834E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGAGCG 20 0.002074986 71.24448 2 TCGGAAG 4845 0.0 66.6615 3 CGGAAGA 4855 0.0 66.2293 4 GATCGGA 5040 0.0 64.18314 1 ATCGGAA 5155 0.0 62.560616 2 AACGCCG 85 0.0 61.56252 1 GAAGAGC 5310 0.0 60.367702 6 GGAAGAG 5930 0.0 54.21624 5 TTGTGTT 26310 0.0 48.529327 1 CTGACCG 40 5.349778E-4 47.571037 1 GCCGTCT 3640 0.0 43.65444 50-51 TGCCGTC 3670 0.0 43.03871 50-51 TCGTATG 3780 0.0 42.90639 44-45 CGTATGC 3760 0.0 42.822475 46-47 CGAGTGG 100 6.2755134E-10 42.81393 1 GGCCGGC 100 6.2755134E-10 42.81393 1 CGTCTTC 3705 0.0 42.76081 52-53 TATGCCG 3760 0.0 42.63662 48-49 ATGCCGT 3730 0.0 42.53383 48-49 CTCGTAT 3760 0.0 42.376762 44-45 >>END_MODULE