FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_35008-1.341000000357b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_35008-1.341000000357b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2356005
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT323651.373723739975085TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC49840.2115445425625158No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG38650.16404888784191884No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC38180.16205398545419045No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA37830.16056841984630763No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA31950.13561091763387598No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG30510.12949887627572945No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA30050.12754641861965488No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC26810.11379432556382521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCG200.00207498671.244482
TCGGAAG48450.066.66153
CGGAAGA48550.066.22934
GATCGGA50400.064.183141
ATCGGAA51550.062.5606162
AACGCCG850.061.562521
GAAGAGC53100.060.3677026
GGAAGAG59300.054.216245
TTGTGTT263100.048.5293271
CTGACCG405.349778E-447.5710371
GCCGTCT36400.043.6544450-51
TGCCGTC36700.043.0387150-51
TCGTATG37800.042.9063944-45
CGTATGC37600.042.82247546-47
CGAGTGG1006.2755134E-1042.813931
GGCCGGC1006.2755134E-1042.813931
CGTCTTC37050.042.7608152-53
TATGCCG37600.042.6366248-49
ATGCCGT37300.042.5338348-49
CTCGTAT37600.042.37676244-45
Produced by FastQC (version 0.11.2)