##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_35005-1.3410000003578e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2583735 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68962142015338 33.0 33.0 33.0 33.0 33.0 2 32.67164705358715 33.0 33.0 33.0 33.0 33.0 3 32.73379429391946 33.0 33.0 33.0 33.0 33.0 4 36.69128296826106 37.0 37.0 37.0 37.0 37.0 5 36.69099114266749 37.0 37.0 37.0 37.0 37.0 6 36.58073254416571 37.0 37.0 37.0 37.0 37.0 7 36.53537572545172 37.0 37.0 37.0 37.0 37.0 8 36.47297691133185 37.0 37.0 37.0 37.0 37.0 9 36.47437953195664 37.0 37.0 37.0 37.0 37.0 10-11 36.35893135325411 37.0 37.0 37.0 37.0 37.0 12-13 36.395486572732885 37.0 37.0 37.0 37.0 37.0 14-15 38.29897125672718 40.0 37.0 40.0 37.0 40.0 16-17 38.26626221342359 40.0 37.0 40.0 37.0 40.0 18-19 38.20041645137756 40.0 37.0 40.0 37.0 40.0 20-21 38.18367557044357 40.0 37.0 40.0 37.0 40.0 22-23 38.12459172477054 40.0 37.0 40.0 37.0 40.0 24-25 38.10670308681037 40.0 37.0 40.0 37.0 40.0 26-27 38.026477754104036 40.0 37.0 40.0 37.0 40.0 28-29 37.92565588189191 40.0 37.0 40.0 33.0 40.0 30-31 37.82257042614664 40.0 37.0 40.0 33.0 40.0 32-33 37.7344812838778 40.0 37.0 40.0 33.0 40.0 34-35 37.66696062096152 40.0 37.0 40.0 33.0 40.0 36-37 37.61159929327117 40.0 37.0 40.0 33.0 40.0 38-39 37.50716307980501 40.0 37.0 40.0 33.0 40.0 40-41 37.358896519960446 40.0 37.0 40.0 33.0 40.0 42-43 37.31536748157222 40.0 37.0 40.0 33.0 40.0 44-45 37.205249377354875 40.0 37.0 40.0 33.0 40.0 46-47 37.12131623405651 40.0 37.0 40.0 33.0 40.0 48-49 36.96788776712782 37.0 37.0 40.0 33.0 40.0 50-51 36.81016861249316 37.0 37.0 40.0 33.0 40.0 52-53 36.64135157049775 37.0 37.0 40.0 33.0 40.0 54-55 36.32687485365179 37.0 37.0 40.0 33.0 40.0 56-57 36.19500238995098 37.0 37.0 40.0 33.0 40.0 58-59 36.01898724133861 37.0 37.0 40.0 33.0 40.0 60-61 35.74405598871401 37.0 37.0 40.0 30.0 40.0 62-63 35.486337414634235 37.0 33.0 40.0 27.0 40.0 64-65 35.23953482071498 37.0 33.0 37.0 27.0 40.0 66-67 34.821653923486735 37.0 33.0 37.0 27.0 40.0 68-69 34.56057838748943 37.0 33.0 37.0 27.0 40.0 70-71 34.30182758680747 37.0 33.0 37.0 27.0 40.0 72-73 33.976808767152974 37.0 33.0 37.0 27.0 37.0 74-75 33.60696569114093 37.0 33.0 37.0 27.0 37.0 76-77 30.904289526596187 33.0 30.0 35.0 24.5 37.0 78-79 33.344465860469434 35.0 33.0 37.0 27.0 37.0 80-81 33.723441258488194 37.0 33.0 37.0 27.0 37.0 82-83 33.63945122081019 37.0 33.0 37.0 27.0 37.0 84-85 33.53781657174594 37.0 33.0 37.0 27.0 37.0 86-87 33.42140293025407 37.0 33.0 37.0 27.0 37.0 88-89 33.263140956793166 37.0 33.0 37.0 27.0 37.0 90-91 33.167813649619646 37.0 33.0 37.0 27.0 37.0 92-93 33.01338179031518 37.0 33.0 37.0 27.0 37.0 94-95 32.892285780081934 33.0 33.0 37.0 27.0 37.0 96-97 32.72859174799273 33.0 33.0 37.0 27.0 37.0 98-99 32.599754425279684 33.0 33.0 37.0 27.0 37.0 100-101 30.966104108974022 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15199559088047465 2306 2 0.1780443545220507 2306 3 0.1502751622735019 2306 4 0.0890701949662116 2306 5 0.1237160372445203 2306 6 0.1302147148446977 2306 7 0.21739469424796454 2306 8 0.18927765365169336 2306 9 0.12981066044681455 2306 10-11 0.21091029773609904 2306 12-13 0.2535824469703485 2306 14-15 0.41431643130583495 2306 16-17 0.39930211084564604 2306 18-19 0.5117286400420582 2306 20-21 0.4850331255977878 2306 22-23 0.47561921471252333 2306 24-25 0.3893604663950825 2306 26-27 0.4606599866538801 2306 28-29 0.4542750116819505 2306 30-31 0.5534054653329008 2306 32-33 0.5247917009267766 2306 34-35 0.5027032662080799 2306 36-37 0.49837091622896423 2306 38-39 0.48282463884100224 2306 40-41 0.6752543120976853 2306 42-43 0.5963710057765539 2306 44-45 0.5218186099557514 2306 46-47 0.5348823635805857 2306 48-49 0.5287299286188158 2306 50-51 0.540753942788406 2306 52-53 0.5851765234196833 2306 54-55 0.6771390689447685 2306 56-57 0.6541870556635203 2306 58-59 0.5202245325469903 2306 60-61 0.6518995299085972 2306 62-63 0.5169354988948953 2306 64-65 0.592112334989757 2306 66-67 0.8182511910734078 2306 68-69 0.5496062840170453 2306 70-71 0.5977955882327564 2306 72-73 0.7631577797283526 2306 74-75 0.730794203653268 2306 76-77 0.39961760317030937 2306 78-79 0.6688467100129429 2306 80-81 0.6539299020329992 2306 82-83 0.7043730704551265 2306 84-85 0.7760077620779029 2306 86-87 0.8014630335747341 2306 88-89 0.7974558372171145 2306 90-91 0.7482259591726859 2306 92-93 0.7798559833957057 2306 94-95 0.7049084402115895 2306 96-97 0.6631317866310269 2306 98-99 0.7039564872589921 2306 100-101 0.7211130132389449 2307 1 0.11807869803956805 2307 2 0.17361989843152514 2307 3 0.09883607782342096 2307 4 0.1395864371442599 2307 5 0.10328563429060722 2307 6 0.1335412094563182 2307 7 0.14685862919194648 2307 8 0.20055589547539654 2307 9 0.12815062280388645 2307 10-11 0.238460631669426 2307 12-13 0.2001198626524996 2307 14-15 0.31523888841832104 2307 16-17 0.4129411239128231 2307 18-19 0.41680645510898984 2307 20-21 0.35184251527397237 2307 22-23 0.4284719630826501 2307 24-25 0.4279008144252501 2307 26-27 0.3820853851014334 2307 28-29 0.4358986645864391 2307 30-31 0.5190236849200573 2307 32-33 0.39406598331034814 2307 34-35 0.38467715752632614 2307 36-37 0.45909465669306115 2307 38-39 0.4788727735133236 2307 40-41 0.4711161579363008 2307 42-43 0.5096492527357199 2307 44-45 0.4722494751011226 2307 46-47 0.42950450922738526 2307 48-49 0.45843715600931034 2307 50-51 0.40162070583055254 2307 52-53 0.4396608076263391 2307 54-55 0.5289529136330344 2307 56-57 0.5405233923854027 2307 58-59 0.369117070465947 2307 60-61 0.37869276856115874 2307 62-63 0.34331661683331305 2307 64-65 0.32430846336600183 2307 66-67 0.45333575927103453 2307 68-69 0.47781215949753175 2307 70-71 0.4579480087037311 2307 72-73 0.5575539651082124 2307 74-75 0.5623672722707269 2307 76-77 0.21340734200646239 2307 78-79 0.49192726741583925 2307 80-81 0.5462214472674276 2307 82-83 0.5340768314096991 2307 84-85 0.5145293656768288 2307 86-87 0.5334936717327565 2307 88-89 0.43881735053464155 2307 90-91 0.47493959818574893 2307 92-93 0.322696124210033 2307 94-95 0.4922559491280296 2307 96-97 0.6423240557086274 2307 98-99 0.5954141620764943 2307 100-101 0.4084594950632656 2201 1 0.12567111979306844 2201 2 0.1708896804334188 2201 3 0.13748735013235347 2201 4 0.13868920273203145 2201 5 0.1692725875861001 2201 6 0.10477387325572352 2201 7 0.1378106229347793 2201 8 0.18830546657137148 2201 9 0.16202469731012314 2201 10-11 0.18172303251100885 2201 12-13 0.17991032411860175 2201 14-15 0.23764600175290695 2201 16-17 0.20523352251188243 2201 18-19 0.29086067842350616 2201 20-21 0.24964974912101923 2201 22-23 0.2067031692464738 2201 24-25 0.204166856165223 2201 26-27 0.1680686555653068 2201 28-29 0.1457839452929548 2201 30-31 0.2838301038038651 2201 32-33 0.2537312968706331 2201 34-35 0.3308816075537635 2201 36-37 0.375406524162166 2201 38-39 0.2715140839450356 2201 40-41 0.4806290761063323 2201 42-43 0.3187013839466246 2201 44-45 0.2087159804767822 2201 46-47 0.32558746683288575 2201 48-49 0.3101262675899932 2201 50-51 0.21597898609403643 2201 52-53 0.2596420151575529 2201 54-55 0.42473755276111547 2201 56-57 0.347708241361957 2201 58-59 0.27174947464395416 2201 60-61 0.203275578488757 2201 62-63 0.22550782416397652 2201 64-65 0.12300263063583117 2201 66-67 0.22757066011717342 2201 68-69 0.37293828736471824 2201 70-71 0.19190991509781696 2201 72-73 0.21929112214451862 2201 74-75 0.2336246402129447 2201 76-77 0.09294877933177759 2201 78-79 0.3328125326252973 2201 80-81 0.2898375685893768 2201 82-83 0.24724418511286217 2201 84-85 0.2931889061250743 2201 86-87 0.3922717783926615 2201 88-89 0.3619843897385735 2201 90-91 0.3784279872386236 2201 92-93 0.234136672003892 2201 94-95 0.2600515920829807 2201 96-97 0.3254296125910514 2201 98-99 0.1550695392841419 2201 100-101 0.2682059403103132 1101 1 0.0743965220730729 1101 2 0.17619987688056682 1101 3 0.08508178429696045 1101 4 0.07056886839376375 1101 5 0.05783793110930446 1101 6 0.10551878975481088 1101 7 0.10963931137982996 1101 8 0.07503549645168306 1101 9 0.11818253428341308 1101 10-11 0.199724205304868 1101 12-13 0.11808685552637854 1101 14-15 0.22408358603178158 1101 16-17 0.2831824884121943 1101 18-19 0.3150347036327119 1101 20-21 0.3210715985550223 1101 22-23 0.33434843842022843 1101 24-25 0.29506779105424386 1101 26-27 0.22167336547867933 1101 28-29 0.18546925676090353 1101 30-31 0.36793602833684247 1101 32-33 0.30746002011471063 1101 34-35 0.24573652241328148 1101 36-37 0.22836205670427034 1101 38-39 0.36780656635313136 1101 40-41 0.42434668206767157 1101 42-43 0.32844908269419903 1101 44-45 0.3307752124222816 1101 46-47 0.32531831817080814 1101 48-49 0.28716037899369695 1101 50-51 0.33940027339005496 1101 52-53 0.3127529721640343 1101 54-55 0.5062866695687092 1101 56-57 0.5713467297461108 1101 58-59 0.472088095866674 1101 60-61 0.40072069474759076 1101 62-63 0.2380758328235757 1101 64-65 0.058218526760761335 1101 66-67 0.3689387467399641 1101 68-69 0.3726509445040378 1101 70-71 0.24665563130512425 1101 72-73 0.12026825517380502 1101 74-75 0.18792541415651698 1101 76-77 -0.06430033312593864 1101 78-79 0.28789586623747 1101 80-81 0.3636223634257405 1101 82-83 0.29745557242259935 1101 84-85 0.34373364461379907 1101 86-87 0.37543252476469746 1101 88-89 0.1678801047747598 1101 90-91 0.22108706727301808 1101 92-93 0.15395860308175457 1101 94-95 0.05150341384507584 1101 96-97 0.3534704278249876 1101 98-99 0.24956748814965124 1101 100-101 0.27760355758134025 2304 1 0.12803883349184986 2304 2 0.1650102005921994 2304 3 0.11972500817280007 2304 4 0.13649746640599147 2304 5 0.03068102907302972 2304 6 0.02672671131983151 2304 7 0.11939923971272748 2304 8 0.14469141008941477 2304 9 0.1339571044411585 2304 10-11 0.08031686955196449 2304 12-13 0.09622450398423155 2304 14-15 0.13082813554040484 2304 16-17 0.22022990719442959 2304 18-19 0.3059713885420834 2304 20-21 0.27273620468932336 2304 22-23 0.2858304371997562 2304 24-25 0.20072143069901927 2304 26-27 0.09905320307600363 2304 28-29 0.271776062623978 2304 30-31 0.3355098896302309 2304 32-33 0.3797920493674454 2304 34-35 0.3081707499493831 2304 36-37 0.3090831064820705 2304 38-39 0.22094213753154435 2304 40-41 0.4287979537298341 2304 42-43 0.2630994176289363 2304 44-45 0.3067449540937446 2304 46-47 0.28573986483384317 2304 48-49 0.22906544326568934 2304 50-51 0.24169113579531398 2304 52-53 0.12581830617703815 2304 54-55 0.2931867850847638 2304 56-57 0.3047939044335237 2304 58-59 0.17229831654524475 2304 60-61 0.36246087907088054 2304 62-63 0.21369050463854222 2304 64-65 0.047966138275889136 2304 66-67 0.253996595590543 2304 68-69 0.317387099098454 2304 70-71 0.28087306120612965 2304 72-73 0.13572920444605785 2304 74-75 0.2781794374119997 2304 76-77 0.22620564963206746 2304 78-79 0.38487070970721504 2304 80-81 0.401989424691763 2304 82-83 0.31465409347693907 2304 84-85 0.292740782339159 2304 86-87 0.39847138940685056 2304 88-89 0.3425060688739521 2304 90-91 0.28064083286855634 2304 92-93 0.25999048149501647 2304 94-95 0.3332789293098912 2304 96-97 0.36664463060379404 2304 98-99 0.472820728029248 2304 100-101 0.3620783962202019 2202 1 0.08493382115614168 2202 2 0.17729199352717728 2202 3 0.09902184001256842 2202 4 0.07420138777953156 2202 5 0.06900410348862351 2202 6 0.14953843335485573 2202 7 0.16228398105126018 2202 8 0.08906961361804377 2202 9 -0.04506947865022681 2202 10-11 0.15092480863657443 2202 12-13 0.1630784364688651 2202 14-15 0.13851341191399769 2202 16-17 0.14234628583282927 2202 18-19 0.19085035211205792 2202 20-21 0.10391967890210196 2202 22-23 0.031904532319586565 2202 24-25 0.09302992927550946 2202 26-27 0.06738711901016359 2202 28-29 0.11570856321943523 2202 30-31 0.13324666720742329 2202 32-33 0.21787318038984438 2202 34-35 0.27207326389411435 2202 36-37 0.20535799049839198 2202 38-39 0.17325923057865822 2202 40-41 0.262640641575139 2202 42-43 0.1971865140581528 2202 44-45 0.1708184174862737 2202 46-47 0.19958065146732906 2202 48-49 0.1801438223194438 2202 50-51 0.20428443838648036 2202 52-53 0.2384988924809761 2202 54-55 0.30301615664380677 2202 56-57 0.492534759329736 2202 58-59 0.46454170925775173 2202 60-61 0.19140032033097043 2202 62-63 0.1531978282945019 2202 64-65 0.13645265128845807 2202 66-67 0.21471440237244366 2202 68-69 0.09149466902519521 2202 70-71 0.14750677589913863 2202 72-73 0.201271708679009 2202 74-75 0.16779440477305485 2202 76-77 -0.06605060108953253 2202 78-79 0.31174685728453255 2202 80-81 0.3176153463671554 2202 82-83 0.3700756597101389 2202 84-85 0.3209666839028529 2202 86-87 0.25268586348146727 2202 88-89 0.19854469539738773 2202 90-91 0.10075347257526346 2202 92-93 0.020769261842801257 2202 94-95 0.20098509063729608 2202 96-97 0.34060929041013566 2202 98-99 0.3617764785654245 2202 100-101 0.3721660807481548 1102 1 0.08404375757083926 1102 2 0.14397304252303655 1102 3 0.11620512362724611 1102 4 0.05796961495403252 1102 5 0.09337670995678593 1102 6 0.007874165138311184 1102 7 0.05484145218572678 1102 8 0.14307383656457517 1102 9 0.13410504262666478 1102 10-11 0.14868441344566463 1102 12-13 0.09198050553183634 1102 14-15 0.07596760905103395 1102 16-17 0.10730555738711445 1102 18-19 0.18940793341617024 1102 20-21 0.139453994151701 1102 22-23 0.012266625455801261 1102 24-25 0.0339878727665095 1102 26-27 0.02213979234025487 1102 28-29 0.15938890247496573 1102 30-31 0.2554454825656052 1102 32-33 0.08185594369572158 1102 34-35 0.13059149554739946 1102 36-37 0.07775851844205306 1102 38-39 0.07525487502672235 1102 40-41 0.1668805321288005 1102 42-43 0.1975896043669607 1102 44-45 0.013628935573265721 1102 46-47 0.08706244961678067 1102 48-49 0.15684863480752398 1102 50-51 0.13636384692616588 1102 52-53 0.08080393615787784 1102 54-55 0.12686954261077688 1102 56-57 0.15584007977020775 1102 58-59 0.14018720208860458 1102 60-61 0.09697388309845678 1102 62-63 0.014894738873074687 1102 64-65 -0.01573926423520078 1102 66-67 0.11246709227820872 1102 68-69 0.10029007444865101 1102 70-71 0.016252199798621803 1102 72-73 -0.07889681739344923 1102 74-75 -0.08921547382770001 1102 76-77 -0.47366406033773245 1102 78-79 0.00757064076802294 1102 80-81 0.12071481365171621 1102 82-83 0.015814781671444678 1102 84-85 0.08905304886877019 1102 86-87 0.08455970014944114 1102 88-89 -0.0312457461970439 1102 90-91 -0.05398054893326787 1102 92-93 -0.12194379461646321 1102 94-95 -0.10183459932388672 1102 96-97 -0.009641278562511957 1102 98-99 0.11357581845167886 1102 100-101 -0.015350291917922476 2305 1 0.03568498838100709 2305 2 0.0829047114507162 2305 3 0.06078283737440415 2305 4 0.04180186630655669 2305 5 0.0058793296764036995 2305 6 -0.05293815501087096 2305 7 0.010136172211424821 2305 8 -0.010723156813028822 2305 9 -0.023138215438621046 2305 10-11 0.08418542619475744 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11 13.0 12 42.0 13 86.0 14 247.0 15 582.0 16 1094.0 17 1892.0 18 2568.0 19 3431.0 20 4509.0 21 5766.0 22 7334.0 23 9271.0 24 11363.0 25 14461.0 26 18334.0 27 23616.0 28 31835.0 29 41829.0 30 47682.0 31 56597.0 32 76009.0 33 106876.0 34 163986.0 35 286720.0 36 572443.0 37 887304.0 38 207362.0 39 435.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.442066547685684 26.0275289531586 21.825231307400443 14.70517319175527 2 32.60361453477233 22.7368518830298 31.15710395996493 13.502429622232931 3 37.80121198891352 18.80014877963159 26.235569556219463 17.16306967523543 4 31.74694537321683 18.631926665732518 31.674677892963615 17.946450068087035 5 31.23369850236189 17.150133430866553 33.94136782603479 17.67480024073676 6 27.073983980555283 17.8758270488266 37.22846963794662 17.821719332671503 7 33.87274623752049 18.786562863451554 27.69448105165584 19.646209847372116 8 31.028336884394104 26.695849226023565 23.43893626861888 18.83687762096345 9 29.098998155770616 25.514652237942357 25.61779749084175 19.768552115445274 10-11 24.091228396100995 28.253419952123572 30.532833282050987 17.12251836972445 12-13 23.81490361821162 27.0602828850482 30.00981137771482 19.115002119025366 14-15 22.76825990281511 27.756929406460028 30.752979697995343 18.72183099272952 16-17 22.510880566311947 27.949035021006413 31.28405196353341 18.25603244914823 18-19 21.604460209735134 27.922310144035666 31.613768439874836 18.859461206354368 20-21 22.097622240670965 28.0887745840808 31.221661664218658 18.591941511029574 22-23 21.423577265508147 28.605208631472976 30.977785802350517 18.993428300668356 24-25 21.719313319670942 27.421407381174927 31.367709923811848 19.491569375342284 26-27 21.54104426344033 27.659609054334133 30.976280462199103 19.823066220026433 28-29 21.430467907893032 28.702614625725936 30.426591736381635 19.4403257299994 30-31 20.596926542389216 28.958910259759612 30.643951488833025 19.800211709018146 32-33 20.306126595800265 29.570079748890656 30.940228003258845 19.18356565205023 34-35 21.092449496562146 28.94660249599901 31.02736929290349 18.933578714535354 36-37 20.473170375820448 29.159017006677 31.768084047790772 18.59972856971178 38-39 21.16999853310405 29.17400598447103 30.39429698721246 19.26169849521246 40-41 21.019325107544486 28.601530742976223 30.816362708828287 19.562781440651005 42-43 20.903253199322513 29.0585453571506 30.814650621286294 19.223550822240593 44-45 20.519919735465308 28.470106801991633 31.39834187822804 19.611631584315013 46-47 21.163078234257867 28.382814460914606 30.685156203585134 19.768951101242386 48-49 20.806372205609254 29.867846286157068 30.072227051722788 19.253554456510884 50-51 21.50065467645669 29.104751716421173 29.590892043600714 19.803701563521418 52-53 20.85224520194498 28.88591602152929 29.480141110555635 20.781697665970096 54-55 21.833148731083753 28.962737961720165 29.57595166298817 19.628161644207907 56-57 21.255878643694427 29.14417400882123 29.500256003499842 20.0996913439845 58-59 21.033237901881034 29.255504986972454 29.97610890915288 19.735148201993628 60-61 21.107185990513507 29.51066722339756 29.933654944151723 19.44849184193721 62-63 20.632285265907427 29.282881440658397 29.692988049798025 20.391845243636148 64-65 21.13343850283836 28.977895243508822 29.670864350498956 20.217801903153866 66-67 21.211851381538448 29.13834354759407 29.404837029953658 20.244968040913825 68-69 21.292821518893007 29.654717855853534 29.24951583071448 19.802944794538977 70-71 20.526388432633357 30.77741619325249 28.973489902809668 19.72270547130449 72-73 20.39595906723344 30.508802348970747 28.912933180044202 20.182305403751606 74-75 20.915156809130313 29.700072869646966 29.03938677985081 20.345383541371916 76-77 20.830701784098444 29.235368408828073 29.30984859115741 20.624081215916075 78-79 20.65884047884564 29.33976604271089 29.485249062982515 20.516144415460953 80-81 20.125217692902332 29.706937643926995 29.457919028111274 20.709925635059406 82-83 19.558501382695034 29.43101552655531 29.641017959262513 21.369465131487146 84-85 19.756492947240847 29.284056208406025 29.943676244659773 21.01577459969336 86-87 20.022250687856765 29.295738820984564 30.543105335731983 20.138905155426688 88-89 20.208931235479056 29.896823412189967 29.89705566983492 19.997189682496057 90-91 20.506117373751803 29.6119446781684 29.369471799341547 20.51246614873825 92-93 19.932823260468822 30.028947412748753 29.620922681113974 20.41730664566845 94-95 19.577991489193618 30.388675132292754 29.635280752631477 20.398052625882155 96-97 19.890341655767045 30.963991360838328 29.176956453390268 19.968710530004362 98-99 20.441062936976294 30.234036160671767 28.759012484871104 20.56588841748083 100-101 20.442687789884946 30.008167449953316 28.270759774176497 21.278384985985245 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 308.0 1 205.5 2 105.5 3 159.0 4 192.0 5 148.5 6 119.5 7 126.5 8 147.0 9 151.0 10 140.0 11 139.0 12 146.0 13 147.5 14 152.0 15 160.5 16 176.0 17 191.5 18 224.5 19 262.0 20 312.5 21 445.0 22 659.0 23 978.0 24 1484.0 25 2334.5 26 4024.5 27 6685.5 28 9461.5 29 12242.5 30 15544.0 31 18709.0 32 24253.5 33 35248.5 34 51750.0 35 75683.5 36 110701.5 37 149842.5 38 182580.0 39 199664.0 40 199914.0 41 186239.0 42 168331.0 43 159200.5 44 146185.5 45 130773.5 46 122487.5 47 109604.0 48 91699.0 49 76224.5 50 62502.0 51 51228.0 52 41545.5 53 33126.5 54 27315.5 55 21518.0 56 15098.5 57 10003.0 58 6799.0 59 4888.0 60 3658.5 61 2643.0 62 1854.5 63 1342.0 64 1006.0 65 716.0 66 511.0 67 372.0 68 313.5 69 241.0 70 168.5 71 119.0 72 75.0 73 55.5 74 39.5 75 27.5 76 20.5 77 10.0 78 6.5 79 6.0 80 5.5 81 3.5 82 1.5 83 0.5 84 0.0 85 0.5 86 1.0 87 2.0 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1567885251389945 2 0.0 3 0.0018577756619777182 4 0.010720913715996415 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.7416646831041108E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 5.612030645557691E-4 38-39 0.0018577756619777182 40-41 0.005921659922553977 42-43 0.0024383305563457553 44-45 0.0011030542992992703 46-47 0.001528794555169164 48-49 0.0029801817910892563 50-51 6.192585539925728E-4 52-53 0.014339705890890512 54-55 0.027247376375673205 56-57 0.02951154046370855 58-59 0.0214611792618051 60-61 0.03094357586981637 62-63 0.005360456857998208 64-65 0.011088598482429506 66-67 0.028834226420279167 68-69 0.027189320886236396 70-71 0.03148542710455987 72-73 0.03160153808343348 74-75 0.040290509669141765 76-77 0.019255070663206558 78-79 0.010527395417873738 80-81 0.015249241892067104 82-83 0.007489158137347677 84-85 0.010759617375620952 86-87 0.034581719874522736 88-89 0.01536535287094071 90-91 0.02165469755992778 92-93 0.05006318372433705 94-95 0.057610397351121535 96-97 0.041683841415625054 98-99 0.03595569979119376 100-101 0.03566542234400974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2583735.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.8390795931268 #Duplication Level Percentage of deduplicated Percentage of total 1 86.9300024700363 40.717213047247384 2 5.822945893410565 5.4548285233585645 3 2.1110525721578046 2.966392783577234 4 1.1448311930735233 2.1449135749226027 5 0.6020906188499726 1.4100685209294421 6 0.5265561103690686 1.4798042138294436 7 0.3904711907998913 1.2802517829289375 8 0.3212445282132771 1.203743842067052 9 0.1523819793792757 0.642368849463369 >10 1.6452158125597625 15.174863334759634 >50 0.19288485608371697 6.23420920071788 >100 0.14378063827293663 14.390500261446308 >500 0.014185835116167576 4.248446539507736 >1k 0.0021065805278867537 1.4404215525379147 >5k 1.664807675267875E-4 0.3992866521619082 >10k+ 8.324038247970849E-5 0.8126873205446568 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 20844 0.8067390812138241 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5187 0.20075588247246717 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5054 0.19560829574240393 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.2574025586989377E-4 0.0 0.0 2 4.644439154944296E-4 0.0 0.0 3 5.031475751189654E-4 0.0 0.0 4 5.418512347435012E-4 0.0 0.0 5 5.805548943680369E-4 0.0 0.0 6 6.579622136171086E-4 0.0 0.0 7 7.74073192490716E-4 0.0 0.0 8 0.0011224061291115381 0.0 0.0 9 0.0014320354061078246 0.0 0.0 10-11 0.00193518298122679 0.0 0.0 12-13 0.0029221263016524527 0.0 0.0 14-15 0.00414129157982533 0.0 0.0 16-17 0.005786197113868102 0.0 0.0 18-19 0.007566565456596749 0.0 0.0 20-21 0.01004359967256704 0.0 0.0 22-23 0.012656096697223205 0.0 3.87036596245358E-5 24-25 0.01536535287094071 0.0 3.87036596245358E-5 26-27 0.01851970113034038 0.0 3.87036596245358E-5 28-29 0.0220030304965486 0.0 3.87036596245358E-5 30-31 0.0314660752747476 0.0 3.87036596245358E-5 32-33 0.03516227476889077 0.0 3.87036596245358E-5 34-35 0.039400325497777444 0.0 3.87036596245358E-5 36-37 0.043290043290043295 0.0 3.87036596245358E-5 38-39 0.048011889764236655 0.0 3.87036596245358E-5 40-41 0.053178828324112186 0.0 3.87036596245358E-5 42-43 0.05944882118328698 0.0 3.87036596245358E-5 44-45 0.0654091847654655 0.0 3.87036596245358E-5 46-47 0.0718533440929507 0.0 3.87036596245358E-5 48-49 0.07911028027255118 0.0 3.87036596245358E-5 50-51 0.08630916096271482 0.0 3.87036596245358E-5 52-53 0.09432081850499374 0.0 3.87036596245358E-5 54-55 0.10378386328319274 0.0 3.87036596245358E-5 56-57 0.11388551844519659 0.0 3.87036596245358E-5 58-59 0.12538050535368372 0.0 3.87036596245358E-5 60-61 0.13724317702860395 0.0 3.87036596245358E-5 62-63 0.15053788410963198 0.0 3.87036596245358E-5 64-65 0.1647034235322121 0.0 3.87036596245358E-5 66-67 0.17997201725409145 0.0 3.87036596245358E-5 68-69 0.19680810919076452 0.0 3.87036596245358E-5 70-71 0.21513429202298223 0.0 3.87036596245358E-5 72-73 0.23466028830356056 0.0 3.87036596245358E-5 74-75 0.25927581582476533 0.0 3.87036596245358E-5 76-77 0.28542013790113924 0.0 3.87036596245358E-5 78-79 0.3144091789599166 0.0 3.87036596245358E-5 80-81 0.348971547004627 0.0 3.87036596245358E-5 82-83 0.38632057854230406 0.0 3.87036596245358E-5 84-85 0.4315264529837619 0.0 3.87036596245358E-5 86-87 0.48410537458369374 0.0 3.87036596245358E-5 88-89 0.5396064224852781 0.0 3.87036596245358E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGCCG 30 1.2933243E-4 63.41436 1 CGGAAGA 4135 0.0 51.79672 4 TCGGAAG 4160 0.0 51.716763 3 CGACGGG 120 0.0 51.524166 1 GAAGAGC 4400 0.0 48.568275 6 GATCGGA 4545 0.0 47.717735 1 ATCGGAA 4615 0.0 46.51502 2 CGAGGCG 155 0.0 46.02655 1 GCGCCCC 65 2.5933969E-6 43.839375 2 GTGACGC 55 4.9277172E-5 43.237064 1 GCCGTCT 2560 0.0 42.03248 50-51 TGCCGTC 2560 0.0 41.75412 50-51 TATGCCG 2575 0.0 41.597095 48-49 CGTATGC 2585 0.0 41.247234 46-47 ATGCCGT 2620 0.0 40.882645 48-49 TCGTATG 2650 0.0 40.681602 44-45 CCGTCTT 2710 0.0 39.531803 52-53 CTCGTAT 2695 0.0 39.47365 44-45 CGTCTTC 2750 0.0 38.956795 52-53 TCTCGTA 2770 0.0 38.66205 42-43 >>END_MODULE