FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_35005-1.3410000003578e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_35005-1.3410000003578e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583735
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT208440.8067390812138241TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC51870.20075588247246717No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG50540.19560829574240393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCCG301.2933243E-463.414361
CGGAAGA41350.051.796724
TCGGAAG41600.051.7167633
CGACGGG1200.051.5241661
GAAGAGC44000.048.5682756
GATCGGA45450.047.7177351
ATCGGAA46150.046.515022
CGAGGCG1550.046.026551
GCGCCCC652.5933969E-643.8393752
GTGACGC554.9277172E-543.2370641
GCCGTCT25600.042.0324850-51
TGCCGTC25600.041.7541250-51
TATGCCG25750.041.59709548-49
CGTATGC25850.041.24723446-47
ATGCCGT26200.040.88264548-49
TCGTATG26500.040.68160244-45
CCGTCTT27100.039.53180352-53
CTCGTAT26950.039.4736544-45
CGTCTTC27500.038.95679552-53
TCTCGTA27700.038.6620542-43
Produced by FastQC (version 0.11.2)