FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_34344-1.34100000035857.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_34344-1.34100000035857.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4672105
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT987252.1130732293045638TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC77300.16545004874676403No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA71910.15391349295446058No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG57510.12309226783216559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG164150.066.742053
CGGAAGA163350.066.4853444
GATCGGA165550.066.323741
ATCGGAA168000.065.240822
GAAGAGC171850.063.1644976
GGAAGAG196750.055.3395585
CGACGGG1600.050.524641
TTGTGTT560350.045.3249661
GCCGTCT123400.043.54426250-51
ATGCCGT123550.043.48394448-49
TCTCGTA124800.043.07332642-43
TCGTATG127500.042.92493444-45
CGTCTTC125350.042.81115752-53
TATGCCG125300.042.74394248-49
TGCCGTC123850.042.7339150-51
CGTATGC125800.042.7175546-47
AATCTCG124950.042.56738340-41
CTCGTAT125500.042.28464544-45
ATCTCGT125700.042.00936542-43
GTATGCC129600.041.9963946-47
Produced by FastQC (version 0.11.2)