##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_34311-1.34100000035764.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2534681 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68195208785642 33.0 33.0 33.0 33.0 33.0 2 32.66503043183738 33.0 33.0 33.0 33.0 33.0 3 32.72687924042513 33.0 33.0 33.0 33.0 33.0 4 36.688476774789414 37.0 37.0 37.0 37.0 37.0 5 36.69202041598134 37.0 37.0 37.0 37.0 37.0 6 36.58212493011941 37.0 37.0 37.0 37.0 37.0 7 36.540743391377454 37.0 37.0 37.0 37.0 37.0 8 36.472732860663726 37.0 37.0 37.0 37.0 37.0 9 36.47693812357453 37.0 37.0 37.0 37.0 37.0 10-11 36.356186636503764 37.0 37.0 37.0 37.0 37.0 12-13 36.39766226992667 37.0 37.0 37.0 37.0 37.0 14-15 38.30083233353625 40.0 37.0 40.0 37.0 40.0 16-17 38.26852807118529 40.0 37.0 40.0 37.0 40.0 18-19 38.20839407404719 40.0 37.0 40.0 37.0 40.0 20-21 38.202148317677846 40.0 37.0 40.0 37.0 40.0 22-23 38.14520426830832 40.0 37.0 40.0 37.0 40.0 24-25 38.13026372943972 40.0 37.0 40.0 37.0 40.0 26-27 38.05104429314774 40.0 37.0 40.0 37.0 40.0 28-29 37.95181938082149 40.0 37.0 40.0 33.0 40.0 30-31 37.845074981822165 40.0 37.0 40.0 33.0 40.0 32-33 37.76298398102168 40.0 37.0 40.0 33.0 40.0 34-35 37.70445136883103 40.0 37.0 40.0 33.0 40.0 36-37 37.65301787483317 40.0 37.0 40.0 33.0 40.0 38-39 37.54784448220506 40.0 37.0 40.0 33.0 40.0 40-41 37.3945944282535 40.0 37.0 40.0 33.0 40.0 42-43 37.374542989827916 40.0 37.0 40.0 33.0 40.0 44-45 37.25981316781086 40.0 37.0 40.0 33.0 40.0 46-47 37.20864459866942 40.0 37.0 40.0 33.0 40.0 48-49 37.06363187320219 38.5 37.0 40.0 33.0 40.0 50-51 36.88796321904019 37.0 37.0 40.0 33.0 40.0 52-53 36.738673229491205 37.0 37.0 40.0 33.0 40.0 54-55 36.43320776855154 37.0 37.0 40.0 33.0 40.0 56-57 36.30387729264551 37.0 37.0 40.0 33.0 40.0 58-59 36.12313995331168 37.0 37.0 40.0 33.0 40.0 60-61 35.85157382723901 37.0 37.0 40.0 33.0 40.0 62-63 35.615295770947114 37.0 35.0 40.0 30.0 40.0 64-65 35.3761670600758 37.0 33.0 37.0 27.0 40.0 66-67 34.95405496786381 37.0 33.0 37.0 27.0 40.0 68-69 34.720421820339524 37.0 33.0 37.0 27.0 40.0 70-71 34.43444579416503 37.0 33.0 37.0 27.0 40.0 72-73 34.12557931353098 37.0 33.0 37.0 27.0 37.0 74-75 33.77464955156093 37.0 33.0 37.0 27.0 37.0 76-77 31.094033134741615 33.0 30.0 35.0 24.5 37.0 78-79 33.503977423589006 35.0 33.0 37.0 27.0 37.0 80-81 33.89694008831881 37.0 33.0 37.0 27.0 37.0 82-83 33.83583811927418 37.0 33.0 37.0 27.0 37.0 84-85 33.743017760420344 37.0 33.0 37.0 27.0 37.0 86-87 33.652717837076935 37.0 33.0 37.0 27.0 37.0 88-89 33.52289361067527 37.0 33.0 37.0 27.0 37.0 90-91 33.43955274845237 37.0 33.0 37.0 27.0 37.0 92-93 33.28795083089351 37.0 33.0 37.0 27.0 37.0 94-95 33.18243656696839 37.0 33.0 37.0 27.0 37.0 96-97 33.03525098424615 35.0 33.0 37.0 27.0 37.0 98-99 32.91420853353933 33.0 33.0 37.0 27.0 37.0 100-101 31.35028826112635 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.18304957526953558 2306 2 0.23708078490082585 2306 3 0.12241525468632375 2306 4 0.15279432351205458 2306 5 0.16499231692240102 2306 6 0.1301633418205128 2306 7 0.19002891435228264 2306 8 0.15895724038973924 2306 9 0.23663050999903135 2306 10-11 0.26060187525684597 2306 12-13 0.23881656122488693 2306 14-15 0.39797505303287295 2306 16-17 0.5186772756020588 2306 18-19 0.6303910357259142 2306 20-21 0.5484749000779559 2306 22-23 0.5389323779932056 2306 24-25 0.5048030579103937 2306 26-27 0.5201279632954652 2306 28-29 0.45848699992289 2306 30-31 0.5588939293978257 2306 32-33 0.5378750018654728 2306 34-35 0.5137208701490792 2306 36-37 0.5173062766944057 2306 38-39 0.4783396056401372 2306 40-41 0.5245347690498647 2306 42-43 0.549659613189128 2306 44-45 0.5096993093225919 2306 46-47 0.49952270285159983 2306 48-49 0.44358813479590253 2306 50-51 0.5523285700864164 2306 52-53 0.43844160998101955 2306 54-55 0.7745737641222874 2306 56-57 0.641434805643577 2306 58-59 0.5959681096634668 2306 60-61 0.49254300972951626 2306 62-63 0.5742236208577864 2306 64-65 0.49842750424542004 2306 66-67 0.633978792652421 2306 68-69 0.5517737191215346 2306 70-71 0.31924690054308513 2306 72-73 0.31106160587394527 2306 74-75 0.4010809243836704 2306 76-77 0.21397564916196288 2306 78-79 0.5029806183470598 2306 80-81 0.7051087561775162 2306 82-83 0.581153267327835 2306 84-85 0.5173182587930043 2306 86-87 0.702616340503603 2306 88-89 0.7995548201853353 2306 90-91 0.6361552225242164 2306 92-93 0.6252126109796308 2306 94-95 0.6113029248651856 2306 96-97 0.6623556901059473 2306 98-99 0.5445234962025367 2306 100-101 0.42335183217234373 2307 1 0.09313568470602718 2307 2 0.17008794405496275 2307 3 0.14369928185747938 2307 4 0.12852811288775 2307 5 0.09702478123257663 2307 6 0.09848780994197881 2307 7 0.13559364270222574 2307 8 0.19171956385446265 2307 9 0.20041779412736105 2307 10-11 0.1452857692789422 2307 12-13 0.1841782851087359 2307 14-15 0.2839364398062756 2307 16-17 0.3426870991043742 2307 18-19 0.4832100637378005 2307 20-21 0.4007819740659144 2307 22-23 0.32595642821254245 2307 24-25 0.4054805596841149 2307 26-27 0.40644512052207915 2307 28-29 0.3487005442168254 2307 30-31 0.4843803540722291 2307 32-33 0.5480337691293897 2307 34-35 0.42222292958495444 2307 36-37 0.4625316389924379 2307 38-39 0.479023464119372 2307 40-41 0.6642524219628072 2307 42-43 0.5898876480375534 2307 44-45 0.5673590711033469 2307 46-47 0.4936352315763841 2307 48-49 0.6440584626515289 2307 50-51 0.520311963364172 2307 52-53 0.542540523052736 2307 54-55 0.6075485929355224 2307 56-57 0.6993833532388933 2307 58-59 0.5190236071200971 2307 60-61 0.24936143077854922 2307 62-63 0.41854585251252985 2307 64-65 0.39898539059299054 2307 66-67 0.5515323222644213 2307 68-69 0.5446818913841511 2307 70-71 0.4014674686548503 2307 72-73 0.35530051684042974 2307 74-75 0.40580539195831733 2307 76-77 0.16541622900286868 2307 78-79 0.405160148114895 2307 80-81 0.6383425011246189 2307 82-83 0.5590917398866893 2307 84-85 0.7045902205270025 2307 86-87 0.6336836792039051 2307 88-89 0.5335608706753447 2307 90-91 0.5852906422239883 2307 92-93 0.38910918943162187 2307 94-95 0.5975252783226139 2307 96-97 0.6988407873908784 2307 98-99 0.5596428860681684 2307 100-101 0.41928319889136745 2201 1 0.17563089348018224 2201 2 0.18504998594215039 2201 3 0.1294525242073874 2201 4 0.20492482094842757 2201 5 0.14610470268729614 2201 6 0.17733679159117344 2201 7 0.0869987365413536 2201 8 0.1337715683728078 2201 9 0.10979699268265364 2201 10-11 0.25352033174075217 2201 12-13 0.18849129494140016 2201 14-15 0.290999078398265 2201 16-17 0.34528079771315134 2201 18-19 0.3894130920463752 2201 20-21 0.46452386252508404 2201 22-23 0.4501756442465208 2201 24-25 0.38278048407612886 2201 26-27 0.273809755926564 2201 28-29 0.3077158453161246 2201 30-31 0.40456134183533976 2201 32-33 0.417229677232136 2201 34-35 0.4863029688938596 2201 36-37 0.4389798402656311 2201 38-39 0.39975474026260116 2201 40-41 0.5761926825837307 2201 42-43 0.37097304880523296 2201 44-45 0.3751262057417719 2201 46-47 0.3999067059684833 2201 48-49 0.4296205092528851 2201 50-51 0.4872792488559554 2201 52-53 0.5234612905917047 2201 54-55 0.678893889375054 2201 56-57 0.5610125694723038 2201 58-59 0.49900970032644665 2201 60-61 0.5322377618508938 2201 62-63 0.46752378476994494 2201 64-65 0.4959109375723898 2201 66-67 0.5640783533888012 2201 68-69 0.4900782350034376 2201 70-71 0.5442206149082622 2201 72-73 0.49919820853025954 2201 74-75 0.4661054849724664 2201 76-77 0.29181982700043463 2201 78-79 0.45489823985621314 2201 80-81 0.5647031276737167 2201 82-83 0.5834036980974062 2201 84-85 0.7102735118861219 2201 86-87 0.5903378170642881 2201 88-89 0.5703631059159235 2201 90-91 0.567312439808525 2201 92-93 0.5517615612924018 2201 94-95 0.6075963715924146 2201 96-97 0.6533205155647366 2201 98-99 0.6843330655510229 2201 100-101 0.6172655352689667 1101 1 0.1424826955235261 1101 2 0.21851911567453897 1101 3 0.16959926198156694 1101 4 0.12134779075514501 1101 5 0.11484526246768922 1101 6 0.0649278674035969 1101 7 0.16431731158142782 1101 8 0.2087122787969804 1101 9 0.13950288326547167 1101 10-11 0.1604262753172918 1101 12-13 0.1551960508419441 1101 14-15 0.2864355084948116 1101 16-17 0.3168823329319892 1101 18-19 0.3376722655842386 1101 20-21 0.31583565688205084 1101 22-23 0.2063325947322241 1101 24-25 0.19408416377022775 1101 26-27 0.28842742738950733 1101 28-29 0.3030710431260246 1101 30-31 0.4906879808287741 1101 32-33 0.3428557368923322 1101 34-35 0.3951402136545923 1101 36-37 0.2819235669916509 1101 38-39 0.3887900947237526 1101 40-41 0.459697446022183 1101 42-43 0.377388258001929 1101 44-45 0.3666941801768715 1101 46-47 0.34645529029958766 1101 48-49 0.33643051592849815 1101 50-51 0.3563844830704781 1101 52-53 0.21145721441552467 1101 54-55 0.44095809743021874 1101 56-57 0.38812656478167895 1101 58-59 0.4392869808122484 1101 60-61 0.45308953305737276 1101 62-63 0.38550244566212655 1101 64-65 0.23841301192234 1101 66-67 0.34968707588046755 1101 68-69 0.35221462415548643 1101 70-71 0.2302382684401394 1101 72-73 0.1995081760887345 1101 74-75 0.37815347512294295 1101 76-77 0.07111841404404018 1101 78-79 0.40830131589020624 1101 80-81 0.39326774031992073 1101 82-83 0.4817875325011798 1101 84-85 0.49431169591479573 1101 86-87 0.35637155715865987 1101 88-89 0.4841620872716135 1101 90-91 0.3962585158465117 1101 92-93 0.36611680535759206 1101 94-95 0.3295932182378536 1101 96-97 0.5155976692767794 1101 98-99 0.43129834724289395 1101 100-101 0.3601452184030869 2304 1 0.11160668287890019 2304 2 0.16403684206348856 2304 3 0.09586551550167144 2304 4 0.11170591052795942 2304 5 0.10099086131663881 2304 6 0.08583458352408257 2304 7 0.13218712803659827 2304 8 0.11062208948074215 2304 9 0.11509901803788836 2304 10-11 0.18388270170714094 2304 12-13 0.13375305810564697 2304 14-15 0.2608276694726399 2304 16-17 0.237065655788669 2304 18-19 0.2973844217592827 2304 20-21 0.318228721912277 2304 22-23 0.27543963110520053 2304 24-25 0.2598776866787702 2304 26-27 0.24250767011142926 2304 28-29 0.44916202550608375 2304 30-31 0.3775351283719459 2304 32-33 0.36626070494275353 2304 34-35 0.4345694095020036 2304 36-37 0.44278586750230176 2304 38-39 0.4331804798843777 2304 40-41 0.48059482901248174 2304 42-43 0.39825865058869425 2304 44-45 0.35581477733576605 2304 46-47 0.4117624814511771 2304 48-49 0.48133972222901633 2304 50-51 0.3023807215667702 2304 52-53 0.33973276474227987 2304 54-55 0.45931661676190316 2304 56-57 0.5319526234865677 2304 58-59 0.5394133059906139 2304 60-61 0.4218253126461704 2304 62-63 0.3637703781366781 2304 64-65 0.36163706859512956 2304 66-67 0.46430018484198854 2304 68-69 0.5454419499463086 2304 70-71 0.38349810379363447 2304 72-73 0.38644396314403906 2304 74-75 0.4623244358703218 2304 76-77 0.33612079020854324 2304 78-79 0.47135972715938834 2304 80-81 0.5825736379501123 2304 82-83 0.45401491176831144 2304 84-85 0.47858158784219285 2304 86-87 0.6329361681487526 2304 88-89 0.42331260089646605 2304 90-91 0.40092104499363046 2304 92-93 0.4237082725559276 2304 94-95 0.4954139333473293 2304 96-97 0.5632241281459258 2304 98-99 0.5471525102730013 2304 100-101 0.633882143192185 2202 1 0.11789000295627972 2202 2 0.14850079889058065 2202 3 0.038104033985810304 2202 4 -0.013000999373069533 2202 5 0.03369226911957668 2202 6 0.045319268155552095 2202 7 0.018621755811643936 2202 8 0.14037139380710073 2202 9 -0.020884661321431963 2202 10-11 0.14835165852369414 2202 12-13 0.10641556303706778 2202 14-15 0.08358194636235083 2202 16-17 0.10723227990971651 2202 18-19 0.2030439034903324 2202 20-21 0.22835855286199802 2202 22-23 0.10889694448639631 2202 24-25 0.042406731976150525 2202 26-27 -0.07951338047706713 2202 28-29 0.1410590890143908 2202 30-31 0.17228925336323897 2202 32-33 0.21872578413034915 2202 34-35 0.0533827664358526 2202 36-37 0.1465287920952889 2202 38-39 0.29672897450780766 2202 40-41 0.3154527723501488 2202 42-43 0.2623781355686887 2202 44-45 0.28826460339099214 2202 46-47 0.2190957993133722 2202 48-49 0.14433083406211722 2202 50-51 0.3268269780092652 2202 52-53 0.12733015377732926 2202 54-55 0.3319443116401857 2202 56-57 0.4305733015020081 2202 58-59 0.30517920099926954 2202 60-61 0.25290636521779675 2202 62-63 0.06303546407531968 2202 64-65 0.008011970757785036 2202 66-67 0.1907455931850066 2202 68-69 0.22636867794899018 2202 70-71 0.06870351475268421 2202 72-73 0.0613707038049256 2202 74-75 0.05543584280827929 2202 76-77 -0.1291322366838088 2202 78-79 0.23036340014741796 2202 80-81 0.18883797072577835 2202 82-83 0.2876633639867663 2202 84-85 0.17595153009970232 2202 86-87 0.2537734659603572 2202 88-89 0.14419293659196342 2202 90-91 0.04751176948576585 2202 92-93 0.03168125151588441 2202 94-95 0.20177098744637334 2202 96-97 0.35264577493570215 2202 98-99 0.3133575393763195 2202 100-101 0.15734949874628512 1102 1 0.04675058633807794 1102 2 0.13888220009466323 1102 3 0.03529192961304517 1102 4 0.061696885284817427 1102 5 0.02558615673928699 1102 6 0.006997445675622771 1102 7 0.06421043895031886 1102 8 0.025400630118589618 1102 9 0.017311273400260063 1102 10-11 0.1568256564887136 1102 12-13 0.07600898428133718 1102 14-15 0.11836027289034234 1102 16-17 0.08949473551543718 1102 18-19 0.17508582126200167 1102 20-21 0.21742953990961666 1102 22-23 0.08157828244105048 1102 24-25 0.0923380335190842 1102 26-27 0.007088026899175759 1102 28-29 0.06875633099286205 1102 30-31 0.1064232708632673 1102 32-33 0.07238370961009366 1102 34-35 0.07883704707796113 1102 36-37 0.21253563444123813 1102 38-39 0.07683514392461888 1102 40-41 0.14928907956109327 1102 42-43 0.03737108239129583 1102 44-45 0.1610005334479112 1102 46-47 0.01584451369992479 1102 48-49 0.0823928348015528 1102 50-51 0.21303900925305896 1102 52-53 0.24982943318195083 1102 54-55 0.2763150612732659 1102 56-57 0.19162316059124151 1102 58-59 0.16633335544806727 1102 60-61 0.27132763495268364 1102 62-63 0.14606719980880456 1102 64-65 0.16953411264224627 1102 66-67 0.11718966123365249 1102 68-69 0.17660593970691707 1102 70-71 0.06537237800456097 1102 72-73 0.057726579127148625 1102 74-75 0.08921389128321522 1102 76-77 -0.14829416643314275 1102 78-79 0.290789524097967 1102 80-81 0.3349550565260273 1102 82-83 0.2135592702920519 1102 84-85 0.24041186781703772 1102 86-87 0.24803161938249474 1102 88-89 0.14821401158786784 1102 90-91 0.14595650567655838 1102 92-93 0.038063129382351235 1102 94-95 0.057821085885301216 1102 96-97 0.173072591472625 1102 98-99 0.29886883617300697 1102 100-101 0.10152489828204736 2305 1 0.08274634265699632 2305 2 0.1414407092710448 2305 3 0.039624597308595355 2305 4 -0.012385908779414478 2305 5 0.01068157229522626 2305 6 0.02679975317721528 2305 7 -0.01277662435701643 2305 8 -0.013923571356322384 2305 9 -0.06190276149862939 2305 10-11 0.020975488413576215 2305 12-13 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sequence quality scores pass #Quality Count 7 2.0 8 41.0 9 135.0 10 99.0 11 55.0 12 39.0 13 79.0 14 302.0 15 736.0 16 1485.0 17 2352.0 18 2933.0 19 3416.0 20 4296.0 21 5198.0 22 6302.0 23 7995.0 24 10092.0 25 12630.0 26 16481.0 27 21293.0 28 28473.0 29 35161.0 30 40444.0 31 51331.0 32 69420.0 33 98964.0 34 154498.0 35 277285.0 36 572716.0 37 903235.0 38 207090.0 39 103.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.93560477782603 24.348822239091252 25.094493139393037 13.621079843689676 2 32.42644735175748 21.118910032465624 33.644194279280114 12.810448336496783 3 38.672727258380526 17.42095283485611 27.907075836829577 15.999244069933788 4 31.685123975746592 17.131818212307653 34.80111915726243 16.381938654683324 5 30.44631651872563 16.039888254182678 37.34142481834992 16.17237040874177 6 25.309062560535235 16.886898193500485 41.635850823042425 16.168188422921858 7 34.41833508832078 17.7950598122604 30.081024002625973 17.705581096792848 8 30.770499325161627 27.7727650935167 24.422560472106746 17.034175109214928 9 29.19688907598234 26.464868754687476 25.404774802036233 18.93346736729395 10-11 24.67213822962337 27.488705679334007 30.103512039582103 17.73564405146052 12-13 24.401275742391253 25.982243919451797 29.608854131940078 20.007626206216877 14-15 23.202328024710013 26.9083565150802 30.73637274276329 19.152942717446493 16-17 22.26589854896928 27.89741588783756 31.531344575510683 18.305340987682474 18-19 21.015129714547907 28.174511901103138 31.759716508704646 19.05064187564431 20-21 21.545836160777693 28.103981567692966 31.492081840705733 18.858100430823615 22-23 20.85853954143655 28.268400326432193 31.793169660901498 19.07989047122976 24-25 21.646767384140254 26.99921607492225 31.875589078073336 19.47842746286416 26-27 21.393283809678614 27.421636095429758 31.60419397943962 19.580886115452003 28-29 21.058507954255386 28.682504820133186 30.911542714842614 19.34744451076881 30-31 20.19252126796232 28.932615189051404 30.827508471480236 20.04735507150604 32-33 19.64383289258096 29.793256034980338 31.360830021608244 19.20208105083046 34-35 20.233946599197296 29.474162626381784 31.55537126762697 18.736519506793954 36-37 20.468815313105416 30.22661175606801 31.32494208801911 17.979630842807463 38-39 21.041853536000133 30.26416213922588 30.308665562103283 18.385318762670703 40-41 21.196999757548593 29.56755072984975 29.81384900584073 19.42160050676092 42-43 20.452911269499683 29.72919657329912 30.87198912501635 18.945903032184848 44-45 19.873051556210303 29.26909858595917 31.81647098555186 19.04137887227867 46-47 20.536954773359824 29.008770654515594 31.221269404340763 19.23300516778382 48-49 20.557953257374034 30.600551644577077 29.8159317257969 19.025563372251987 50-51 20.78504623488854 29.715433907268256 29.732418393587164 19.76710146425604 52-53 19.96957736808721 29.53956668465324 29.745836864061936 20.74501908319761 54-55 21.17717606081522 29.396922853497536 29.90223645021983 19.523664635467412 56-57 21.04073206225489 29.47545075598747 29.27067064598081 20.21314653577683 58-59 20.579595592805262 29.541905719874563 30.228768937966453 19.64972974935372 60-61 20.410882352012578 29.86575049016048 30.3207472855686 19.402619872258338 62-63 20.38803119384456 29.853304654871092 29.841034176438825 19.917629974845518 64-65 20.96294025317295 29.66121289744215 29.546589835676695 19.829257013708208 66-67 21.161817210993057 29.977777576070658 29.053855931919117 19.806549281017173 68-69 21.93746635705953 30.052577700393535 28.635765938269053 19.37419000427788 70-71 21.01917640194177 31.044949236730623 28.76091290415706 19.174961457170543 72-73 20.303536716588496 31.192332335449276 28.821149064382563 19.682981883579668 74-75 21.098276276491994 30.11787769626581 28.745111288370147 20.03873473887205 76-77 21.3338852582768 29.24372208395599 29.145997432680876 20.27639522508633 78-79 21.148573693411308 29.246934821341025 29.63454204365499 19.96994944159268 80-81 19.745671555099236 29.833558380637985 30.01499043910662 20.40577962515616 82-83 19.143668011312702 29.618630291781876 30.12896844185195 21.10873325505347 84-85 19.095709966832203 29.21502486203759 30.6067623479612 21.082502823169005 86-87 19.2178272215355 29.295189652804414 31.56390747765796 19.923075648002122 88-89 19.533535231969694 29.91315070385021 31.008177881248088 19.545136182932 90-91 19.996085412201754 29.69936991710308 30.025815759451735 20.27872891124343 92-93 19.16168763769919 30.64297021898254 30.118077639891915 20.077264503426356 94-95 18.922849450108924 30.857140826947592 30.379342830868744 19.840666892074736 96-97 19.55702363997006 31.506329995739286 29.440247646491972 19.496398717798684 98-99 20.031171330427348 30.734525287435936 28.845991802232156 20.388311579904556 100-101 19.568960787958655 30.73037967239694 28.559732126893987 21.140927412750415 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1409.0 1 872.5 2 390.0 3 641.0 4 704.0 5 516.0 6 458.5 7 466.0 8 489.0 9 479.5 10 477.0 11 470.5 12 446.5 13 452.0 14 437.0 15 427.5 16 457.0 17 493.5 18 543.5 19 586.5 20 661.5 21 792.0 22 924.5 23 1199.0 24 1619.5 25 2217.0 26 3379.0 27 5181.0 28 7059.5 29 9008.5 30 11398.5 31 14674.0 32 21995.0 33 33358.5 34 48736.5 35 74634.0 36 118899.0 37 177915.0 38 225267.0 39 231341.0 40 211782.0 41 186664.5 42 161858.0 43 150058.0 44 134387.0 45 115375.0 46 110124.0 47 98825.0 48 75115.5 49 57482.0 50 46814.0 51 39522.5 52 33576.5 53 27453.0 54 21948.0 55 15999.5 56 11044.0 57 8284.5 58 6697.0 59 5204.5 60 3947.5 61 3146.0 62 2403.0 63 1739.5 64 1267.0 65 883.0 66 602.0 67 431.0 68 321.5 69 254.0 70 187.5 71 142.5 72 108.5 73 73.0 74 60.0 75 40.5 76 21.0 77 17.5 78 14.0 79 10.0 80 8.5 81 4.0 82 3.5 83 3.5 84 1.5 85 0.5 86 0.0 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16073028519170657 2 0.0 3 0.002169898302784453 4 0.010810038817508002 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.945269641426278E-5 22-23 1.7753713386418253E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 6.115167944210731E-4 38-39 0.0017556449904346937 40-41 0.006016536203175074 42-43 0.002229077347405847 44-45 0.0011835808924278834 46-47 0.0014005707227063287 48-49 0.003077310320312497 50-51 5.917904462139417E-4 52-53 0.015130109074869776 54-55 0.028011414454126575 56-57 0.029333079784004377 58-59 0.021777888420673054 60-61 0.03223285297045269 62-63 0.0056220092390324465 64-65 0.011322923870893419 66-67 0.029747333096354137 68-69 0.028287583329026412 70-71 0.032015863140174246 72-73 0.03233148471148835 74-75 0.04095189887800477 76-77 0.020515402135416645 78-79 0.011263744826272024 80-81 0.01574162586929085 82-83 0.007298748836638615 84-85 0.01163854544220752 86-87 0.035231257897936664 88-89 0.01601779474419069 90-91 0.022488036956129786 92-93 0.05150549516882006 94-95 0.05908041288035851 96-97 0.04219465881505405 98-99 0.037184166370442674 100-101 0.037243345415064064 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2534681.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.46527697362197 #Duplication Level Percentage of deduplicated Percentage of total 1 85.56470558446887 32.91270099473014 2 6.148355343926584 4.7299638247276965 3 2.293442883201168 2.646537471765452 4 1.2291057791460203 1.891115768989244 5 0.7496022224720515 1.4416828553715029 6 0.5856440539681499 1.351617644630382 7 0.38777279702388723 1.044105162825194 8 0.40391039540632445 1.242922018546355 9 0.23981727787222845 0.8302174214773799 >10 1.9460568242037117 14.632703472530705 >50 0.20184034188232827 5.390176840221785 >100 0.21209045837289048 18.43714288247832 >500 0.030395275698875186 7.622851753715748 >1k 0.006848586558579739 4.534797518729086 >5k 3.0913184892835457E-4 0.6201866925100867 >10k+ 1.0304394948349103E-4 0.6712776767508302 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 16936 0.6681708664719544 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5244 0.20688993999639405 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5216 0.20578526449679466 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5187 0.20464113630078107 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 4953 0.19540920533984354 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 4813 0.18988582784184677 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 3989 0.15737680599649423 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 3950 0.15583815083633798 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3613 0.14254259214473142 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3482 0.137374288914463 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT 3314 0.13074623591686688 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 2944 0.11614873824358962 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG 2924 0.11535968431530437 No Hit TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA 2537 0.10009149080298467 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.945269641426278E-4 0.0 0.0 2 4.3397966055689057E-4 0.0 0.0 3 6.312431426282045E-4 0.0 0.0 4 6.312431426282045E-4 0.0 0.0 5 6.312431426282045E-4 0.0 0.0 6 7.496012318709928E-4 0.0 0.0 7 8.285066246995184E-4 0.0 0.0 8 9.863174103565695E-4 0.0 0.0 9 0.001065222803185095 0.0 0.0 10-11 0.001518928811949117 0.0 0.0 12-13 0.001972634820713139 0.0 0.0 14-15 0.0028011414454126573 0.0 0.0 16-17 0.004083354078876198 0.0 0.0 18-19 0.005385293060546869 0.0 0.0 20-21 0.0067069583904246725 0.0 0.0 22-23 0.007910265631059687 0.0 0.0 24-25 0.009310836353766016 0.0 0.0 26-27 0.011007302299579316 0.0 0.0 28-29 0.013177200602363768 0.0 0.0 30-31 0.02014060151948115 0.0 0.0 32-33 0.02238940521509413 0.0 0.0 34-35 0.024145050205528824 0.0 0.0 36-37 0.026176864070863354 0.0 0.0 38-39 0.028761015685997565 0.0 0.0 40-41 0.031680515220653016 0.0 0.0 42-43 0.034678920148136984 0.0 0.0 44-45 0.03771677777203522 0.0 0.0 46-47 0.040991351574419035 0.0 0.0 48-49 0.04489716851943104 0.0 0.0 50-51 0.04894106990189298 7.890539282852556E-5 0.0 52-53 0.053300592855669016 7.890539282852556E-5 0.0 54-55 0.05752203137199513 9.863174103565695E-5 0.0 56-57 0.06340048313772029 1.1835808924278834E-4 0.0 58-59 0.07058087388511611 1.1835808924278834E-4 0.0 60-61 0.07726810592733366 1.1835808924278834E-4 0.0 62-63 0.08462603380859367 1.1835808924278834E-4 0.0 64-65 0.09304918449303877 1.1835808924278834E-4 0.0 66-67 0.1026164633734975 1.1835808924278834E-4 0.0 68-69 0.113801302806941 1.1835808924278834E-4 0.0 70-71 0.12727439863241174 1.1835808924278834E-4 0.0 72-73 0.14145764299333918 1.1835808924278834E-4 0.0 74-75 0.1587379240227863 1.1835808924278834E-4 1.972634820713139E-5 76-77 0.18033827530959518 1.1835808924278834E-4 3.945269641426278E-5 78-79 0.20428606203305266 1.1835808924278834E-4 3.945269641426278E-5 80-81 0.23067991593419446 1.1835808924278834E-4 3.945269641426278E-5 82-83 0.26314948508313274 1.1835808924278834E-4 3.945269641426278E-5 84-85 0.30017584066791836 1.1835808924278834E-4 3.945269641426278E-5 86-87 0.3428044791435293 1.1835808924278834E-4 3.945269641426278E-5 88-89 0.393047488027093 1.1835808924278834E-4 3.945269641426278E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGACG 20 0.0020748668 71.24567 2 TCGGAAG 3545 0.0 53.59335 3 CGGAAGA 3550 0.0 53.115437 4 GATCGGA 3725 0.0 50.817772 1 GAAGAGC 3800 0.0 49.98709 6 ATCGGAA 3855 0.0 49.037224 2 ACGGCCT 80 4.1800376E-9 47.56186 1 CTCGTAT 2065 0.0 43.926754 44-45 TCGTATG 2145 0.0 43.727596 44-45 TCTCGTA 2095 0.0 43.636906 42-43 GGAAGAG 4370 0.0 43.575706 5 GCCGTCT 2115 0.0 43.575687 50-51 CGTATGC 2125 0.0 43.24775 46-47 TATGCCG 2110 0.0 43.223106 48-49 TGCCGTC 2125 0.0 42.699947 50-51 ATGCCGT 2145 0.0 42.628555 48-49 AATCTCG 2150 0.0 42.18928 40-41 ATCTCGT 2180 0.0 41.49977 42-43 CACTCCG 2245 0.0 41.459232 30-31 TTGTGTT 26560 0.0 41.294296 1 >>END_MODULE