FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_34311-1.34100000035764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_34311-1.34100000035764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534681
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT169360.6681708664719544TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG52440.20688993999639405No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52160.20578526449679466No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC51870.20464113630078107No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC49530.19540920533984354No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG48130.18988582784184677No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT39890.15737680599649423No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT39500.15583815083633798No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA36130.14254259214473142No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA34820.137374288914463No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT33140.13074623591686688No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT29440.11614873824358962No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG29240.11535968431530437No Hit
TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA25370.10009149080298467No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG200.002074866871.245672
TCGGAAG35450.053.593353
CGGAAGA35500.053.1154374
GATCGGA37250.050.8177721
GAAGAGC38000.049.987096
ATCGGAA38550.049.0372242
ACGGCCT804.1800376E-947.561861
CTCGTAT20650.043.92675444-45
TCGTATG21450.043.72759644-45
TCTCGTA20950.043.63690642-43
GGAAGAG43700.043.5757065
GCCGTCT21150.043.57568750-51
CGTATGC21250.043.2477546-47
TATGCCG21100.043.22310648-49
TGCCGTC21250.042.69994750-51
ATGCCGT21450.042.62855548-49
AATCTCG21500.042.1892840-41
ATCTCGT21800.041.4997742-43
CACTCCG22450.041.45923230-31
TTGTGTT265600.041.2942961
Produced by FastQC (version 0.11.2)