##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_34310-1.34100000035813.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5636509 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66943812207166 33.0 33.0 33.0 33.0 33.0 2 32.65920164413824 33.0 33.0 33.0 33.0 33.0 3 32.724597086600944 33.0 33.0 33.0 33.0 33.0 4 36.67236670783281 37.0 37.0 37.0 37.0 37.0 5 36.678188041569705 37.0 37.0 37.0 37.0 37.0 6 36.555519027823784 37.0 37.0 37.0 37.0 37.0 7 36.509094902536305 37.0 37.0 37.0 37.0 37.0 8 36.439140255076325 37.0 37.0 37.0 37.0 37.0 9 36.44314681303622 37.0 37.0 37.0 37.0 37.0 10-11 36.3143161840068 37.0 37.0 37.0 37.0 37.0 12-13 36.3478071267162 37.0 37.0 37.0 37.0 37.0 14-15 38.20916510556445 40.0 37.0 40.0 37.0 40.0 16-17 38.13464078563522 40.0 37.0 40.0 37.0 40.0 18-19 38.09521487502282 40.0 37.0 40.0 37.0 40.0 20-21 38.07656130771724 40.0 37.0 40.0 35.0 40.0 22-23 38.01510358628009 40.0 37.0 40.0 35.0 40.0 24-25 37.99209422002165 40.0 37.0 40.0 33.0 40.0 26-27 37.88839359610709 40.0 37.0 40.0 33.0 40.0 28-29 37.783509793029694 40.0 37.0 40.0 33.0 40.0 30-31 37.69137634660035 40.0 37.0 40.0 33.0 40.0 32-33 37.60598714558958 40.0 37.0 40.0 33.0 40.0 34-35 37.506962909134 40.0 37.0 40.0 33.0 40.0 36-37 37.40679816176999 40.0 37.0 40.0 33.0 40.0 38-39 37.29667121972128 40.0 37.0 40.0 33.0 40.0 40-41 37.135620026509315 40.0 37.0 40.0 33.0 40.0 42-43 37.083200967123446 38.5 37.0 40.0 33.0 40.0 44-45 36.955117254314686 37.0 37.0 40.0 33.0 40.0 46-47 36.853113780178475 37.0 37.0 40.0 33.0 40.0 48-49 36.68042941118341 37.0 37.0 40.0 33.0 40.0 50-51 36.49533248327999 37.0 37.0 40.0 33.0 40.0 52-53 36.30553796685147 37.0 37.0 40.0 33.0 40.0 54-55 35.98212528357535 37.0 37.0 40.0 33.0 40.0 56-57 35.80699986463252 37.0 37.0 40.0 27.0 40.0 58-59 35.61911149259231 37.0 33.0 40.0 27.0 40.0 60-61 35.33582470994014 37.0 33.0 40.0 27.0 40.0 62-63 35.08922384404957 37.0 33.0 37.0 27.0 40.0 64-65 34.83719186822908 37.0 33.0 37.0 27.0 40.0 66-67 34.37451434921864 37.0 33.0 37.0 27.0 40.0 68-69 34.097983255238304 37.0 33.0 37.0 27.0 38.5 70-71 33.83330808129642 37.0 33.0 37.0 27.0 37.0 72-73 33.49487049519481 37.0 33.0 37.0 27.0 37.0 74-75 33.10189312214351 37.0 33.0 37.0 27.0 37.0 76-77 30.36918968815627 33.0 30.0 33.0 24.5 37.0 78-79 32.89290826999478 33.0 33.0 37.0 27.0 37.0 80-81 33.370860314425116 37.0 33.0 37.0 27.0 37.0 82-83 33.25722419675015 37.0 33.0 37.0 27.0 37.0 84-85 33.13925596499536 37.0 33.0 37.0 27.0 37.0 86-87 33.00373076668555 37.0 33.0 37.0 27.0 37.0 88-89 32.860075358701636 33.0 33.0 37.0 27.0 37.0 90-91 32.76508154249376 33.0 33.0 37.0 27.0 37.0 92-93 32.5586283105376 33.0 33.0 37.0 27.0 37.0 94-95 32.40069003704243 33.0 33.0 37.0 22.0 37.0 96-97 32.22681122304604 33.0 33.0 37.0 22.0 37.0 98-99 32.08168921578942 33.0 33.0 37.0 22.0 37.0 100-101 30.38349313378192 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15351533070998613 2306 2 0.19472671633310057 2306 3 0.14908703347271768 2306 4 0.16871257048024546 2306 5 0.12505062434230751 2306 6 0.16480386343270226 2306 7 0.18312376869129565 2306 8 0.165514738200919 2306 9 0.18891058577977304 2306 10-11 0.22613578908782728 2306 12-13 0.2200947280093004 2306 14-15 0.3873575303310375 2306 16-17 0.3737207812416514 2306 18-19 0.4610920289298619 2306 20-21 0.47366556525792447 2306 22-23 0.49546309797562316 2306 24-25 0.4283824735874049 2306 26-27 0.39378059400783627 2306 28-29 0.408784433233798 2306 30-31 0.554665752146704 2306 32-33 0.5027393689286797 2306 34-35 0.5030917971128588 2306 36-37 0.4742921022553972 2306 38-39 0.42719717493274345 2306 40-41 0.5442070140340647 2306 42-43 0.47709796156924966 2306 44-45 0.5160771471264596 2306 46-47 0.4941439205381215 2306 48-49 0.5147959019574628 2306 50-51 0.5744597537660141 2306 52-53 0.5681061138806953 2306 54-55 0.5872203207691271 2306 56-57 0.5556366170716629 2306 58-59 0.4782723145159693 2306 60-61 0.5290960497278903 2306 62-63 0.44640364528203946 2306 64-65 0.3163374414362181 2306 66-67 0.4877717782843334 2306 68-69 0.4642730570797582 2306 70-71 0.4279469711659374 2306 72-73 0.36466577529375144 2306 74-75 0.3159509500472595 2306 76-77 0.08152984958934795 2306 78-79 0.44231245219382487 2306 80-81 0.5117940589493557 2306 82-83 0.5518249306737175 2306 84-85 0.4932122949962192 2306 86-87 0.5774041242511458 2306 88-89 0.5728387849432934 2306 90-91 0.535428137452648 2306 92-93 0.4165239610273588 2306 94-95 0.45189808374482254 2306 96-97 0.6544234429023277 2306 98-99 0.5939241954228578 2306 100-101 0.45239652900524163 2307 1 0.1416124530278111 2307 2 0.22955143997610605 2307 3 0.1269817740665573 2307 4 0.1537228081734625 2307 5 0.1415744895661888 2307 6 0.14353182328504488 2307 7 0.19925372924670626 2307 8 0.22266530258420403 2307 9 0.1528813111649896 2307 10-11 0.22564647738211363 2307 12-13 0.1614307525845078 2307 14-15 0.33537904380940375 2307 16-17 0.4135765017763262 2307 18-19 0.4564359939050817 2307 20-21 0.3907801818800394 2307 22-23 0.38698886077980177 2307 24-25 0.3889179846080921 2307 26-27 0.3836295954581814 2307 28-29 0.3473077157850213 2307 30-31 0.41918601018594615 2307 32-33 0.4257484309801498 2307 34-35 0.4185486215838452 2307 36-37 0.44397424732505186 2307 38-39 0.3846310497045877 2307 40-41 0.5592662772818429 2307 42-43 0.49730630070532555 2307 44-45 0.38377207350496434 2307 46-47 0.3924299204084889 2307 48-49 0.41031569177289384 2307 50-51 0.4102470159397953 2307 52-53 0.4454258645432603 2307 54-55 0.4546872074979049 2307 56-57 0.44900197672022557 2307 58-59 0.32061962728486293 2307 60-61 0.2659556526788478 2307 62-63 0.32093629600568363 2307 64-65 0.2609472705457492 2307 66-67 0.281399921452838 2307 68-69 0.21658061372632886 2307 70-71 0.2588264636625368 2307 72-73 0.3637990266164124 2307 74-75 0.3260216892006511 2307 76-77 0.04599143774590786 2307 78-79 0.26797481992257843 2307 80-81 0.3655621980019248 2307 82-83 0.427156446202126 2307 84-85 0.3948511707468825 2307 86-87 0.3734465175810584 2307 88-89 0.3473807024590698 2307 90-91 0.4179420769125173 2307 92-93 0.3325373540501033 2307 94-95 0.35070428462802283 2307 96-97 0.3583021102320174 2307 98-99 0.3238945258473578 2307 100-101 0.06126816901756271 2201 1 0.13748529246691987 2201 2 0.16907140509790963 2201 3 0.10916090462811923 2201 4 0.14008226161836035 2201 5 0.09659846832725805 2201 6 0.12224008099556016 2201 7 0.14897227073258534 2201 8 0.12962322913291047 2201 9 0.13861094198632173 2201 10-11 0.2437215509388011 2201 12-13 0.17908134008621346 2201 14-15 0.3356849012758758 2201 16-17 0.2568755651444192 2201 18-19 0.35595514185930455 2201 20-21 0.40243956136198733 2201 22-23 0.43844610039702303 2201 24-25 0.37597824050225626 2201 26-27 0.33978925143063776 2201 28-29 0.3898635164908484 2201 30-31 0.4703492319805491 2201 32-33 0.48587501766653673 2201 34-35 0.49927560821804917 2201 36-37 0.451120923333427 2201 38-39 0.4603260135843854 2201 40-41 0.638312614862123 2201 42-43 0.38684805309333825 2201 44-45 0.43547469554499685 2201 46-47 0.4292806378212646 2201 48-49 0.4222221367642831 2201 50-51 0.36456502691677173 2201 52-53 0.4535507040696558 2201 54-55 0.5833198888150903 2201 56-57 0.5804825566863343 2201 58-59 0.4760246452509378 2201 60-61 0.38180581339620545 2201 62-63 0.38144598803101104 2201 64-65 0.38930856865358265 2201 66-67 0.4480043139142822 2201 68-69 0.4734515537151154 2201 70-71 0.3455461672907205 2201 72-73 0.374757777761495 2201 74-75 0.34659423116290355 2201 76-77 0.14756246562664543 2201 78-79 0.3386887179465603 2201 80-81 0.38419640454381465 2201 82-83 0.36246927512948446 2201 84-85 0.37896817242904746 2201 86-87 0.352279497814429 2201 88-89 0.25670609348072304 2201 90-91 0.4024014996671923 2201 92-93 0.23462820033391552 2201 94-95 0.196570229215709 2201 96-97 0.4100256837986862 2201 98-99 0.18797257045820714 2201 100-101 0.20279642792596775 1101 1 0.06909860607110829 1101 2 0.14955915966334743 1101 3 0.10273635928422209 1101 4 0.10284775178159578 1101 5 0.07455034373558789 1101 6 0.11701925594684326 1101 7 0.09175183731117187 1101 8 0.12082418669039896 1101 9 0.14097553551187048 1101 10-11 0.15245617397391698 1101 12-13 0.10022165083057644 1101 14-15 0.23409096993913892 1101 16-17 0.28728107387095747 1101 18-19 0.29061613896130467 1101 20-21 0.338737880792344 1101 22-23 0.2691417735516026 1101 24-25 0.25014690481710744 1101 26-27 0.27091759783770897 1101 28-29 0.30948157621565286 1101 30-31 0.3392209214931654 1101 32-33 0.2376371865921456 1101 34-35 0.3603131352681501 1101 36-37 0.31732475446652586 1101 38-39 0.3665502347889529 1101 40-41 0.4547096067210816 1101 42-43 0.32452648417852714 1101 44-45 0.4022715300551667 1101 46-47 0.4042294984382764 1101 48-49 0.37182539346961363 1101 50-51 0.2729747819670294 1101 52-53 0.27143551535476007 1101 54-55 0.4375505566812663 1101 56-57 0.4257958010578733 1101 58-59 0.3163006142154998 1101 60-61 0.31068715196519037 1101 62-63 0.23221055926582324 1101 64-65 0.24296092942936554 1101 66-67 0.4166313082014099 1101 68-69 0.32255583967365453 1101 70-71 0.19219811304007095 1101 72-73 0.11429047073902865 1101 74-75 0.16019082096757842 1101 76-77 -0.006819716644667295 1101 78-79 0.27502947393093535 1101 80-81 0.39340688143627034 1101 82-83 0.44450221287408453 1101 84-85 0.41408152164503775 1101 86-87 0.4522981709244718 1101 88-89 0.370721825075627 1101 90-91 0.30956412290510116 1101 92-93 0.30529760900746794 1101 94-95 0.3506514044424449 1101 96-97 0.392827487747347 1101 98-99 0.16888969366419815 1101 100-101 0.13215852595635624 2304 1 0.08180688075135834 2304 2 0.17956332116257556 2304 3 0.11535285509251025 2304 4 0.11607616795281928 2304 5 0.0981095990953591 2304 6 0.09588743467386962 2304 7 0.13650237049178315 2304 8 0.08861384292228536 2304 9 0.05914757769323842 2304 10-11 0.11649171163465866 2304 12-13 0.11641855567420123 2304 14-15 0.19710929559500556 2304 16-17 0.246758360367771 2304 18-19 0.33503888061797227 2304 20-21 0.24150413455968334 2304 22-23 0.24353480254454496 2304 24-25 0.11814616030175529 2304 26-27 0.1929269977572332 2304 28-29 0.3075253180535782 2304 30-31 0.3407052691322505 2304 32-33 0.360543306034522 2304 34-35 0.30619486714969213 2304 36-37 0.3181229873169187 2304 38-39 0.32671379676783374 2304 40-41 0.451786249930187 2304 42-43 0.32180185917717097 2304 44-45 0.3151370868172947 2304 46-47 0.26381181871467163 2304 48-49 0.34464461452731854 2304 50-51 0.26524196435841674 2304 52-53 0.25489229333694396 2304 54-55 0.5310691639637497 2304 56-57 0.5974059927274169 2304 58-59 0.4246953415996515 2304 60-61 0.410179443550021 2304 62-63 0.32011619295509064 2304 64-65 0.2732340686781569 2304 66-67 0.33748291237120753 2304 68-69 0.20830953199414637 2304 70-71 0.1801019194161313 2304 72-73 0.14235692268261602 2304 74-75 0.32106472111326667 2304 76-77 0.144024208218422 2304 78-79 0.290541388839479 2304 80-81 0.3392679068289439 2304 82-83 0.26536376625927005 2304 84-85 0.2886013239352607 2304 86-87 0.3610391420229888 2304 88-89 0.3057748437878658 2304 90-91 0.3475585098396863 2304 92-93 0.260661420639579 2304 94-95 0.3098804655909575 2304 96-97 0.25874675117300683 2304 98-99 0.2544254674064632 2304 100-101 0.3281465679819888 2202 1 0.0834749911423458 2202 2 0.13295053200149454 2202 3 0.09169528264662574 2202 4 0.05463128566977815 2202 5 0.08964815697130746 2202 6 0.05444732836009791 2202 7 0.09897771411556988 2202 8 0.10655342218970532 2202 9 0.04113842135006962 2202 10-11 0.1300731568200959 2202 12-13 0.1591492546206581 2202 14-15 0.13196073859870694 2202 16-17 0.11175441932341101 2202 18-19 0.20365220204368484 2202 20-21 0.18079347679292113 2202 22-23 0.14090905067727277 2202 24-25 0.13299527495831853 2202 26-27 -0.003109022294871977 2202 28-29 0.10745823829787327 2202 30-31 0.1649488544985971 2202 32-33 0.22958999324630014 2202 34-35 0.09603743410644228 2202 36-37 0.2170689940152002 2202 38-39 0.2155542301932627 2202 40-41 0.23517622645786673 2202 42-43 0.18277319491290456 2202 44-45 0.20351874639953138 2202 46-47 0.19632044005983573 2202 48-49 0.1858441332527505 2202 50-51 0.15744638727372973 2202 52-53 0.24526500033744014 2202 54-55 0.37962565085815925 2202 56-57 0.4737621866029471 2202 58-59 0.32834059641823643 2202 60-61 0.24439976035432665 2202 62-63 0.17854840028284258 2202 64-65 0.23778138034419527 2202 66-67 0.2562408050207381 2202 68-69 0.2929916045163239 2202 70-71 0.23721034833096866 2202 72-73 0.2260774648669539 2202 74-75 0.18289309732845282 2202 76-77 0.08228088997834249 2202 78-79 0.34686018006700436 2202 80-81 0.4441359663292701 2202 82-83 0.3928932035745447 2202 84-85 0.3301574127494078 2202 86-87 0.3840126848429577 2202 88-89 0.39576272743710206 2202 90-91 0.2845757256931236 2202 92-93 0.1907527977040928 2202 94-95 0.3114099188095736 2202 96-97 0.5077845374888881 2202 98-99 0.41534413578074947 2202 100-101 0.3455103348252351 1102 1 0.1113634343276928 1102 2 0.1494945810797077 1102 3 0.1163971324525761 1102 4 0.12660837094352217 1102 5 0.12500822149026902 1102 6 0.08310491910082618 1102 7 0.024246899599788208 1102 8 0.09832744459985321 1102 9 0.12163909732004186 1102 10-11 0.19952087290472065 1102 12-13 0.13280614096853327 1102 14-15 0.18549847323709656 1102 16-17 0.1654907338069691 1102 18-19 0.28991418408917724 1102 20-21 0.24650895645783066 1102 22-23 0.13197892739806605 1102 24-25 0.20627279680352473 1102 26-27 0.1489123190021857 1102 28-29 0.27941210997746424 1102 30-31 0.33393106292321306 1102 32-33 0.31181452098464746 1102 34-35 0.24613672402902154 1102 36-37 0.26078557219073417 1102 38-39 0.20164833669707605 1102 40-41 0.28374716452091064 1102 42-43 0.126008124280645 1102 44-45 0.14187325049270072 1102 46-47 0.09966066902838122 1102 48-49 0.188494171305166 1102 50-51 0.19440197288083993 1102 52-53 0.20453751937743192 1102 54-55 0.23060477592429862 1102 56-57 0.18952807646024183 1102 58-59 0.1444764778855543 1102 60-61 0.20914133373408816 1102 62-63 0.13560051337582735 1102 64-65 0.12492776040187437 1102 66-67 0.2876935900721733 1102 68-69 0.2655629844171514 1102 70-71 0.08734051366461415 1102 72-73 -0.07442005892128378 1102 74-75 0.14984528549963017 1102 76-77 -0.07901465291651988 1102 78-79 0.16573300322219353 1102 80-81 0.3251689009249219 1102 82-83 0.2492510571935398 1102 84-85 0.2812892798429303 1102 86-87 0.36672547754860574 1102 88-89 0.27052088114075445 1102 90-91 0.247308724302016 1102 92-93 0.1471829537789091 1102 94-95 0.1580153441744443 1102 96-97 0.16151836140664244 1102 98-99 0.23732132562022912 1102 100-101 0.2845197436504634 2305 1 0.06548518967350958 2305 2 0.11297061615847781 2305 3 0.05448363310788551 2305 4 0.04293160930850348 2305 5 0.054377102716799186 2305 6 0.0028459624918895088 2305 7 0.0768435905143221 2305 8 0.024463942176538467 2305 9 0.06296484256796475 2305 10-11 0.11876168319965785 2305 12-13 0.11746225820269274 2305 14-15 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quality scores pass #Quality Count 7 1.0 8 17.0 9 47.0 10 60.0 11 28.0 12 67.0 13 170.0 14 444.0 15 1133.0 16 2204.0 17 3756.0 18 5533.0 19 7630.0 20 10403.0 21 13549.0 22 17427.0 23 22293.0 24 28230.0 25 36006.0 26 46641.0 27 60093.0 28 80445.0 29 102233.0 30 115498.0 31 140522.0 32 189902.0 33 269643.0 34 414922.0 35 725905.0 36 1357754.0 37 1711348.0 38 272546.0 39 59.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.98100500485559 24.70351583775134 22.92076370915507 15.394715448237994 2 31.679289432519315 22.061634249142507 32.45327914849422 13.80579716984396 3 39.48597923577813 18.744324861179763 24.88580529576721 16.883890607274893 4 31.257636327181686 18.234812957669508 32.784848533239646 17.72270218190916 5 34.26237765255054 16.806519780239864 31.406407760548237 17.524694806661355 6 28.046313773294784 17.769828807157054 36.434945814865195 17.74891160468297 7 34.50930354231671 18.649202902009026 27.501455244726834 19.34003831094743 8 30.137342103064146 25.98223474849415 24.876195531666852 19.004227616774852 9 31.65863835221411 23.644777290340528 24.803260315915402 19.89332404152996 10-11 26.208855516774655 28.01342107322103 28.280962560336548 17.496760849667766 12-13 25.874324504256492 26.23327898822471 27.936993264536014 19.95540324298278 14-15 25.505521236637783 26.71030064885907 27.700816232174912 20.08336188232823 16-17 24.883584857222797 27.096647942902248 28.515389578904248 19.50437762097071 18-19 23.42975944862325 27.260499362282577 29.11881272610405 20.190928462990122 20-21 23.363090538713166 27.860814838128444 28.983425192841096 19.792669430317293 22-23 23.276253605516327 28.430469819757004 28.396441622836292 19.896834951890373 24-25 23.504468812167246 26.766248399496924 29.443366452532942 20.285916335802888 26-27 22.656772126151132 26.674116904630154 29.34561977990277 21.32349118931594 28-29 22.400718245992334 27.892291132685145 28.31648986988223 21.390500751440296 30-31 20.977550111247936 28.461934505914922 28.775284489033904 21.785230893803238 32-33 21.01394675321196 28.066769697342806 28.84383755973777 22.07544598970746 34-35 22.005668757026733 28.093160145756883 29.03281978259948 20.868351314616902 36-37 21.322025550734573 29.320166941306546 29.29185135666424 20.06595615129464 38-39 21.26879087206785 28.573036736924994 28.765996320177717 21.39217607082944 40-41 21.75240525446036 27.843679130024086 27.749537720613986 22.654377894901568 42-43 22.38206201044537 28.44023984927896 28.31182393358282 20.86587420669285 44-45 21.277443948248923 28.632676672149437 28.241658790836706 21.848220588764935 46-47 22.01624908197841 28.36560088488455 28.449572305505168 21.168577727631877 48-49 22.11158474509814 28.29012196952412 28.71543448693351 20.882858798444236 50-51 21.749682070177332 28.300642033728945 27.558643149321988 22.391032746771735 52-53 21.093199656616783 28.432394920511893 27.83938814192274 22.63501728094858 54-55 22.05173407729881 28.513014619180723 28.220176703631576 21.21507459988889 56-57 21.998435651181268 28.24774742199663 27.514491078642024 22.23932584818008 58-59 21.357990701715362 27.92035370356059 28.164518186611794 22.55713740811225 60-61 21.08530107464768 27.856214129865826 28.92523607882305 22.13324871666344 62-63 20.830837184565972 27.90535082921818 28.805561210979242 22.458250775236607 64-65 21.92424286782691 28.42362495464349 28.25768887161867 21.39444330591094 66-67 22.16040727470972 28.726792701013093 27.695624373660028 21.41717565061716 68-69 21.775321075038935 27.85165858934347 28.660565114783644 21.71245522083395 70-71 21.37727294749476 29.276671134727017 28.403589189979645 20.942466727798582 72-73 22.367975861323288 29.52449117529016 27.67981220898902 20.427720754397534 74-75 23.402665150255025 28.447688945352027 27.438469432343087 20.711176472049857 76-77 23.41303486044218 27.17380464566932 27.88088316763796 21.53227732625054 78-79 23.078285901553944 27.39561196674402 28.590989331109974 20.93511280059206 80-81 22.228533258168717 28.44967390960071 28.22107482028852 21.100718011942053 82-83 21.3640936026436 28.444058361972367 28.539753941620816 21.652094093763218 84-85 21.58141384325307 27.899001285244303 28.750924764635812 21.768660106866815 86-87 20.774847450798916 28.574469699356168 29.237163039634616 21.413519810210293 88-89 20.717444683438693 29.092257970414792 29.15129282244794 21.039004523698583 90-91 21.456607034024845 28.232385148035817 28.376361294150975 21.93464652378836 92-93 21.36009632575671 28.556199062462195 27.62369338844948 22.46001122333161 94-95 21.093026981521348 29.57374264264384 27.162975063427584 22.17025531240722 96-97 21.126201485337244 30.388083579860126 26.40705744624509 22.078657488557536 98-99 21.365865908724864 29.054535338400576 27.3087017037274 22.27089704914716 100-101 21.999309087995268 29.026787526278568 27.05163639926053 21.922266986465633 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 219.0 1 137.0 2 127.0 3 291.0 4 296.5 5 183.5 6 152.5 7 171.0 8 181.5 9 190.5 10 193.5 11 183.0 12 185.5 13 169.5 14 166.0 15 186.0 16 202.0 17 239.0 18 287.0 19 325.5 20 356.5 21 438.5 22 593.5 23 778.0 24 1021.0 25 1390.0 26 1907.0 27 2598.5 28 3579.5 29 4852.5 30 6556.5 31 9216.0 32 15140.5 33 26759.5 34 45790.5 35 78724.0 36 123400.0 37 187280.5 38 270562.5 39 323513.5 40 343500.5 41 336618.0 42 346341.0 43 389735.0 44 387753.5 45 368743.5 46 382907.0 47 368553.0 48 304760.5 49 247971.0 50 201476.5 51 166491.5 52 149224.5 53 136450.5 54 108953.0 55 68542.5 56 43743.5 57 34887.0 58 30881.0 59 26775.0 60 21607.0 61 16934.5 62 12214.0 63 8232.5 64 5821.5 65 4298.0 66 3492.5 67 2926.0 68 2304.5 69 1730.0 70 1228.0 71 885.0 72 667.0 73 495.0 74 333.0 75 202.0 76 134.5 77 85.5 78 49.0 79 38.0 80 29.5 81 14.0 82 9.5 83 9.5 84 7.5 85 6.0 86 4.5 87 2.5 88 1.0 89 1.0 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15921202290282868 2 0.0 3 0.0014725426678108737 4 0.01098197483584254 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.870738962716106E-6 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.322443377629664E-5 22-23 1.241903454780255E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 6.564346832409919E-4 38-39 0.0016943111418787763 40-41 0.005446633723107689 42-43 0.002412840997858781 44-45 0.001170937543078526 46-47 0.0011975497599666745 48-49 0.003246690460354095 50-51 7.983665066444495E-4 52-53 0.014601236332630711 54-55 0.026266258068602395 56-57 0.027969439949443887 58-59 0.020730916955867543 60-61 0.029903261043315994 62-63 0.005792592542653617 64-65 0.010715852666961057 66-67 0.02820894990143722 68-69 0.026514638759558444 70-71 0.030160512473234764 72-73 0.030772593461662175 74-75 0.03823288492930642 76-77 0.018930156946436173 78-79 0.010467471976005005 80-81 0.0155858883574922 82-83 0.0072296522546136275 84-85 0.010919879663103527 86-87 0.03284834637893774 88-89 0.015168963626244541 90-91 0.02129864424948137 92-93 0.04807053443895858 94-95 0.05529131595460949 96-97 0.03961672020749013 98-99 0.034622494171480965 100-101 0.034196698701270595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5636509.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.05097174405266 #Duplication Level Percentage of deduplicated Percentage of total 1 82.40489950722421 23.939424071558697 2 7.200346966670958 4.183541525522665 3 2.951113449549835 2.571981403090981 4 1.5152395625553177 1.7607672686906102 5 0.8939714382611178 1.2985369496456927 6 0.6627773923912061 1.15525963793723 7 0.5432057425301472 1.1046458274215318 8 0.3290015459856779 0.7646251692943656 9 0.3045663390476264 0.7963153298875943 >10 2.452607991173563 14.634232577884196 >50 0.3694827937875396 7.550454816831485 >100 0.31183622691570634 18.83522164330887 >500 0.03854272098343855 7.810375310845533 >1k 0.02166960467881881 10.744835028885998 >5k 4.309237382261386E-4 0.846371058827613 >10k+ 3.0779450664695157E-4 2.00341238036671 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 40638 0.7209781799337143 TruSeq Adapter, Index 7 (97% over 38bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 23833 0.4228326433968259 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 23632 0.4192666063338141 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 12971 0.23012471017078126 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10952 0.1943046662393336 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8028 0.1424285847853698 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7934 0.1407608858603792 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7367 0.13070146787665912 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6813 0.12087268910596968 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6120 0.10857784490364514 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5860 0.10396506064303276 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.7901990398666977E-4 0.0 0.0 2 5.322443377629664E-4 0.0 0.0 3 5.499858156883986E-4 0.0 0.0 4 6.032102494646953E-4 0.0 0.0 5 7.274005949427207E-4 0.0 0.0 6 7.806250287190174E-4 0.0 0.0 7 9.225568521224751E-4 0.0 0.0 8 0.0010822301534513651 0.0 0.0 9 0.0012773864106311193 0.0 0.0 10-11 0.002031399222461988 0.0 0.0 12-13 0.0028829901628827344 0.0 0.0 14-15 0.003814417753967926 0.0 0.0 16-17 0.0050208382528973165 0.0 0.0 18-19 0.00681272752336597 0.0 0.0 20-21 0.008702194922424501 0.0 0.0 22-23 0.011097294442357849 0.0 0.0 24-25 0.013927060171464289 0.0 0.0 26-27 0.016668118510943564 0.0 0.0 28-29 0.019639816063453462 0.0 0.0 30-31 0.032023367655405144 0.0 0.0 32-33 0.03657405674327851 0.0 0.0 34-35 0.04108926287530101 0.0 0.0 36-37 0.04587059117620498 0.0 0.0 38-39 0.051521251895455145 0.0 0.0 40-41 0.0572251370484816 0.0 0.0 42-43 0.0636652935354135 0.0 0.0 44-45 0.07041592588604045 0.0 0.0 46-47 0.07869232533825458 0.0 0.0 48-49 0.0882815941569507 0.0 0.0 50-51 0.09812811440556557 0.0 0.0 52-53 0.11007699978834418 0.0 0.0 54-55 0.12326778862590303 0.0 1.7741477925432213E-5 56-57 0.13816175934430336 0.0 1.7741477925432213E-5 58-59 0.15519357815271828 0.0 1.7741477925432213E-5 60-61 0.17363584445620509 0.0 1.7741477925432213E-5 62-63 0.19423370032763188 0.0 2.661221688814832E-5 64-65 0.21790083188015846 0.0 3.5482955850864425E-5 66-67 0.24406951182017095 0.0 4.435369481358053E-5 68-69 0.27320988931269335 0.0 5.322443377629664E-5 70-71 0.308311403388161 0.0 5.322443377629664E-5 72-73 0.3478926406398003 0.0 5.322443377629664E-5 74-75 0.3901350995802544 0.0 5.322443377629664E-5 76-77 0.438267729191952 0.0 5.322443377629664E-5 78-79 0.4922639172580049 0.0 6.209517273901274E-5 80-81 0.555130844286774 0.0 7.096591170172885E-5 82-83 0.6243935741076614 0.0 7.096591170172885E-5 84-85 0.7020746352041662 0.0 7.096591170172885E-5 86-87 0.7866482604747016 0.0 7.096591170172885E-5 88-89 0.8803232639209837 0.0 7.096591170172885E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTGTT 62085 0.0 46.253452 1 ATGCCGT 5275 0.0 41.423546 48-49 GCCGTCT 5315 0.0 41.385426 50-51 TATGCCG 5260 0.0 41.315903 48-49 TGCCGTC 5310 0.0 41.066517 50-51 TCGTATG 5405 0.0 40.812576 44-45 CGTATGC 5400 0.0 40.545258 46-47 CTCGTAT 5300 0.0 40.456276 44-45 CGGAAGA 10980 0.0 40.3537 4 TCTCGTA 5385 0.0 39.905525 42-43 TCGGAAG 11210 0.0 39.737915 3 GAAGAGC 11195 0.0 39.532776 6 CATCTCG 5430 0.0 39.182644 40-41 ATCTCGT 5420 0.0 39.122116 42-43 CTGCTTG 5870 0.0 37.757774 58-59 CTTGAAA 5570 0.0 37.144867 62-63 GATCGGA 11985 0.0 37.109337 1 CGTCTTC 5940 0.0 36.871754 52-53 GTATGCC 6040 0.0 36.524284 46-47 TCTTCTG 6155 0.0 35.81742 54-55 >>END_MODULE