FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_34310-1.34100000035813.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_34310-1.34100000035813.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5636509
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT406380.7209781799337143TruSeq Adapter, Index 7 (97% over 38bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC238330.4228326433968259No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA236320.4192666063338141No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT129710.23012471017078126No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC109520.1943046662393336No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC80280.1424285847853698No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT79340.1407608858603792No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC73670.13070146787665912No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA68130.12087268910596968No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT61200.10857784490364514No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG58600.10396506064303276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGTT620850.046.2534521
ATGCCGT52750.041.42354648-49
GCCGTCT53150.041.38542650-51
TATGCCG52600.041.31590348-49
TGCCGTC53100.041.06651750-51
TCGTATG54050.040.81257644-45
CGTATGC54000.040.54525846-47
CTCGTAT53000.040.45627644-45
CGGAAGA109800.040.35374
TCTCGTA53850.039.90552542-43
TCGGAAG112100.039.7379153
GAAGAGC111950.039.5327766
CATCTCG54300.039.18264440-41
ATCTCGT54200.039.12211642-43
CTGCTTG58700.037.75777458-59
CTTGAAA55700.037.14486762-63
GATCGGA119850.037.1093371
CGTCTTC59400.036.87175452-53
GTATGCC60400.036.52428446-47
TCTTCTG61550.035.8174254-55
Produced by FastQC (version 0.11.2)