##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_34302-1.341000000358ea.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8142916 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.666598673006085 33.0 33.0 33.0 33.0 33.0 2 32.64865767987782 33.0 33.0 33.0 33.0 33.0 3 32.71905396052225 33.0 33.0 33.0 33.0 33.0 4 36.66712023063974 37.0 37.0 37.0 37.0 37.0 5 36.68086199096245 37.0 37.0 37.0 37.0 37.0 6 36.56414974684744 37.0 37.0 37.0 37.0 37.0 7 36.469963585526365 37.0 37.0 37.0 37.0 37.0 8 36.41682156613184 37.0 37.0 37.0 37.0 37.0 9 36.46668220573564 37.0 37.0 37.0 37.0 37.0 10-11 36.3212716427383 37.0 37.0 37.0 37.0 37.0 12-13 36.35508391588468 37.0 37.0 37.0 37.0 37.0 14-15 38.2551622170731 40.0 37.0 40.0 37.0 40.0 16-17 38.24369648415875 40.0 37.0 40.0 37.0 40.0 18-19 38.143400349457124 40.0 37.0 40.0 37.0 40.0 20-21 38.18744734687181 40.0 37.0 40.0 37.0 40.0 22-23 38.049303099774086 40.0 37.0 40.0 35.0 40.0 24-25 38.0897990351368 40.0 37.0 40.0 37.0 40.0 26-27 38.0410971327716 40.0 37.0 40.0 37.0 40.0 28-29 37.880286742488806 40.0 37.0 40.0 33.0 40.0 30-31 37.82001484480498 40.0 37.0 40.0 33.0 40.0 32-33 37.71766760212189 40.0 37.0 40.0 33.0 40.0 34-35 37.57026315879962 40.0 37.0 40.0 33.0 40.0 36-37 37.31479957548377 40.0 37.0 40.0 33.0 40.0 38-39 37.44045879878904 40.0 37.0 40.0 33.0 40.0 40-41 37.165958607457085 40.0 37.0 40.0 33.0 40.0 42-43 37.07608165183087 38.5 37.0 40.0 33.0 40.0 44-45 37.094539842975166 38.5 37.0 40.0 33.0 40.0 46-47 37.08823006402129 38.5 37.0 40.0 33.0 40.0 48-49 36.83099273036834 37.0 37.0 40.0 33.0 40.0 50-51 36.730293177530015 37.0 37.0 40.0 33.0 40.0 52-53 36.6105736569062 37.0 37.0 40.0 33.0 40.0 54-55 36.2057134078259 37.0 37.0 40.0 33.0 40.0 56-57 36.12689004774211 37.0 37.0 40.0 33.0 40.0 58-59 35.939109343630705 37.0 37.0 40.0 33.0 40.0 60-61 35.642953212338185 37.0 35.0 40.0 27.0 40.0 62-63 35.43244250585417 37.0 33.0 40.0 27.0 40.0 64-65 35.19335610240852 37.0 33.0 37.0 27.0 40.0 66-67 34.543110600674254 37.0 33.0 37.0 27.0 40.0 68-69 34.37291892732284 37.0 33.0 37.0 27.0 40.0 70-71 33.998342178649516 37.0 33.0 37.0 27.0 40.0 72-73 33.31681242935578 37.0 33.0 37.0 24.5 37.0 74-75 32.57135429126372 37.0 33.0 37.0 22.0 37.0 76-77 30.000568530978338 33.0 30.0 35.0 22.0 37.0 78-79 32.17746818215981 35.0 33.0 37.0 22.0 37.0 80-81 32.48997380054025 37.0 33.0 37.0 22.0 37.0 82-83 32.36773914897317 37.0 33.0 37.0 22.0 37.0 84-85 32.254767825186946 37.0 33.0 37.0 22.0 37.0 86-87 32.14166909004096 37.0 33.0 37.0 18.5 37.0 88-89 31.99115501130062 35.0 33.0 37.0 15.0 37.0 90-91 31.88465501793215 33.0 33.0 37.0 15.0 37.0 92-93 31.70246886987413 33.0 33.0 37.0 15.0 37.0 94-95 31.573960667161494 33.0 33.0 37.0 15.0 37.0 96-97 31.424069154096642 33.0 33.0 37.0 15.0 37.0 98-99 31.300108339567792 33.0 33.0 37.0 15.0 37.0 100-101 29.76677979976706 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15907699065001424 2306 2 0.19858173004867297 2306 3 0.1557635093024956 2306 4 0.1724021305405472 2306 5 0.1870988928373265 2306 6 0.18606506799957145 2306 7 0.2140845251998229 2306 8 0.13226749730552712 2306 9 0.20741259817489066 2306 10-11 0.22680090480464088 2306 12-13 0.237691306822974 2306 14-15 0.40790743841928645 2306 16-17 0.4481257369897591 2306 18-19 0.5042231591108077 2306 20-21 0.4430168854603025 2306 22-23 0.43401047755874345 2306 24-25 0.44562573025790897 2306 26-27 0.4352256075476362 2306 28-29 0.4915791459421186 2306 30-31 0.5476670868054683 2306 32-33 0.5019183362098829 2306 34-35 0.49621529114745755 2306 36-37 0.5342409296801591 2306 38-39 0.5341138497092572 2306 40-41 0.6554803051467886 2306 42-43 0.5914067453817182 2306 44-45 0.5270517536686441 2306 46-47 0.5027759467786836 2306 48-49 0.4955681279679567 2306 50-51 0.5131905869461164 2306 52-53 0.5135673470826774 2306 54-55 0.6753226574580111 2306 56-57 0.6017041403933945 2306 58-59 0.4821268887085637 2306 60-61 0.5075178797015312 2306 62-63 0.42154763286618646 2306 64-65 0.34713138936933063 2306 66-67 0.47917309243307926 2306 68-69 0.537492891985714 2306 70-71 0.3902628782344877 2306 72-73 0.241697952552812 2306 74-75 0.27674267143296305 2306 76-77 0.015420563292497746 2306 78-79 0.42068544074033554 2306 80-81 0.5368142730309486 2306 82-83 0.5143931695133048 2306 84-85 0.5280739153909764 2306 86-87 0.5808764137628017 2306 88-89 0.5149549506096278 2306 90-91 0.518889789527524 2306 92-93 0.4579243355959193 2306 94-95 0.4384338443680704 2306 96-97 0.5746221926360313 2306 98-99 0.5313651337423551 2306 100-101 0.3858790521422968 2307 1 0.13274097750573333 2307 2 0.18096070847338552 2307 3 0.14429549566709454 2307 4 0.12919256195424111 2307 5 0.1206214857544694 2307 6 0.14795142220569346 2307 7 0.13214149336014458 2307 8 0.17536259927584297 2307 9 0.09540839057793704 2307 10-11 0.20047277192347224 2307 12-13 0.17591814158723906 2307 14-15 0.29560685180902624 2307 16-17 0.3540617305956175 2307 18-19 0.4164923347605409 2307 20-21 0.43427568911931047 2307 22-23 0.4058389280550756 2307 24-25 0.43626617952098456 2307 26-27 0.3681270937710508 2307 28-29 0.2600160065217807 2307 30-31 0.37690862249028356 2307 32-33 0.39379594042679855 2307 34-35 0.46511980188538615 2307 36-37 0.3666623977882395 2307 38-39 0.4427752554890958 2307 40-41 0.5342223219622255 2307 42-43 0.4805751759815493 2307 44-45 0.4270340636368246 2307 46-47 0.43820328910143047 2307 48-49 0.4540365773934951 2307 50-51 0.5115894449569325 2307 52-53 0.4748082874599717 2307 54-55 0.5612804023283502 2307 56-57 0.657149468837261 2307 58-59 0.52329103650127 2307 60-61 0.3904876287041432 2307 62-63 0.42762154845940614 2307 64-65 0.3420830813284468 2307 66-67 0.4255395436730467 2307 68-69 0.29759399865857716 2307 70-71 0.24073426282626542 2307 72-73 0.08329096048806406 2307 74-75 0.11809454911135475 2307 76-77 -0.07002602773175326 2307 78-79 0.3062591676418194 2307 80-81 0.33068866638830485 2307 82-83 0.32966683743449465 2307 84-85 0.4571701326502904 2307 86-87 0.435015293086245 2307 88-89 0.4768735066997465 2307 90-91 0.3556646539449879 2307 92-93 0.2635888918006444 2307 94-95 0.29899031436249146 2307 96-97 0.47432617287000767 2307 98-99 0.3808224124893371 2307 100-101 0.15801481261984662 2201 1 0.1184427001641879 2201 2 0.13877829543329767 2201 3 0.11591804779855153 2201 4 0.1360260168819849 2201 5 0.08761406114884096 2201 6 0.09104116918083349 2201 7 0.14137188929928612 2201 8 0.10102956968823662 2201 9 0.15369394437903594 2201 10-11 0.23729607945077902 2201 12-13 0.21240588870161758 2201 14-15 0.2867025156465388 2201 16-17 0.28189757525522197 2201 18-19 0.36103390254162804 2201 20-21 0.32256689901736024 2201 22-23 0.3313862264963703 2201 24-25 0.29676800261326974 2201 26-27 0.2453213287952707 2201 28-29 0.32376192871481635 2201 30-31 0.43394524170148685 2201 32-33 0.40473745502492164 2201 34-35 0.42347671087166106 2201 36-37 0.42599049837366465 2201 38-39 0.40764416833617645 2201 40-41 0.5159357084153626 2201 42-43 0.3713871956201089 2201 44-45 0.4090331900932256 2201 46-47 0.3997565313879434 2201 48-49 0.348774702782535 2201 50-51 0.35856316017224543 2201 52-53 0.3615042143698517 2201 54-55 0.5756292209950402 2201 56-57 0.48872118509810747 2201 58-59 0.32974837437046745 2201 60-61 0.3485722891055829 2201 62-63 0.4175919404602908 2201 64-65 0.2914163683858746 2201 66-67 0.4140630582884839 2201 68-69 0.35988969635852897 2201 70-71 0.15851197183099686 2201 72-73 0.2405535506591363 2201 74-75 0.26442569175416253 2201 76-77 0.20602893939656397 2201 78-79 0.4453214996427661 2201 80-81 0.3960024510339579 2201 82-83 0.4218111912861886 2201 84-85 0.3943581049786786 2201 86-87 0.5285191648143694 2201 88-89 0.5118830166049193 2201 90-91 0.583583064025607 2201 92-93 0.5226799965686197 2201 94-95 0.4932579085109623 2201 96-97 0.41232272915970825 2201 98-99 0.3751648479643137 2201 100-101 0.44810881073063413 1101 1 0.05788688201456438 1101 2 0.12323581592417554 1101 3 0.09290944170477644 1101 4 0.08996014755822301 1101 5 0.04762137069456429 1101 6 0.08720329183194053 1101 7 0.10229472127631567 1101 8 0.07316384728707703 1101 9 0.09237077026551077 1101 10-11 0.12675749251254587 1101 12-13 0.11082771461004626 1101 14-15 0.1523822960821235 1101 16-17 0.23859071189177428 1101 18-19 0.27684068541958595 1101 20-21 0.294166496882589 1101 22-23 0.20743884864042172 1101 24-25 0.18798262741186988 1101 26-27 0.20519940425553784 1101 28-29 0.18616027920131017 1101 30-31 0.2589829601281295 1101 32-33 0.21472932599058936 1101 34-35 0.25150922115550856 1101 36-37 0.33334657934499035 1101 38-39 0.2742081001547234 1101 40-41 0.373725807383245 1101 42-43 0.32301358783613665 1101 44-45 0.31468380185610556 1101 46-47 0.27029809263081717 1101 48-49 0.22502451437332383 1101 50-51 0.10514956939265829 1101 52-53 0.18165783555830473 1101 54-55 0.36685333942284615 1101 56-57 0.4268814404858787 1101 58-59 0.3152412309175219 1101 60-61 0.30526332214414253 1101 62-63 0.19473228739665416 1101 64-65 0.10469351065349031 1101 66-67 0.32518009809074755 1101 68-69 0.24594565865034923 1101 70-71 0.11804039272034572 1101 72-73 -0.19685828114667459 1101 74-75 -0.032087146412933976 1101 76-77 -0.16581694406337633 1101 78-79 0.19841854465464337 1101 80-81 0.31310459297231574 1101 82-83 0.33080762138892794 1101 84-85 0.294754843560348 1101 86-87 0.2320839768514915 1101 88-89 0.21322112443498042 1101 90-91 0.2551055055748499 1101 92-93 0.19514924013672896 1101 94-95 0.12452663053311142 1101 96-97 0.2927007146225655 1101 98-99 0.1739760955918186 1101 100-101 0.23266113946849387 2304 1 0.13206159025285302 2304 2 0.1690582965415075 2304 3 0.10744197437212932 2304 4 0.0929029143795077 2304 5 0.08593421582086336 2304 6 0.06590411624677017 2304 7 0.12723365872000159 2304 8 0.10496725514193628 2304 9 0.11022211615672006 2304 10-11 0.1263573098129811 2304 12-13 0.11689033049322717 2304 14-15 0.1596738569241296 2304 16-17 0.16196845490850365 2304 18-19 0.27773501071349926 2304 20-21 0.23656000183195403 2304 22-23 0.2205333998848218 2304 24-25 0.16495318749971233 2304 26-27 0.16452662079902325 2304 28-29 0.26489316952432773 2304 30-31 0.2867349860879429 2304 32-33 0.28994801697351136 2304 34-35 0.24285136389943318 2304 36-37 0.31015310775210736 2304 38-39 0.25262973359009777 2304 40-41 0.34643867918149596 2304 42-43 0.2699368780101068 2304 44-45 0.32853460305707927 2304 46-47 0.29962321777554735 2304 48-49 0.24601312386118934 2304 50-51 0.25298200996567033 2304 52-53 0.2488004016119234 2304 54-55 0.46654656646768444 2304 56-57 0.5128416729686762 2304 58-59 0.391358652533917 2304 60-61 0.4002780992662238 2304 62-63 0.31415425886428494 2304 64-65 0.2513932205380982 2304 66-67 0.23913980424922698 2304 68-69 0.19530224743336788 2304 70-71 0.12959857662511354 2304 72-73 0.05544246431123412 2304 74-75 0.15957403805339965 2304 76-77 0.054067947930104054 2304 78-79 0.32190021232012356 2304 80-81 0.42234702430940985 2304 82-83 0.3341952240933139 2304 84-85 0.33787602373799075 2304 86-87 0.40673178132952614 2304 88-89 0.3185268574818281 2304 90-91 0.24241134385347252 2304 92-93 0.24765244920942564 2304 94-95 0.3659446361196643 2304 96-97 0.3425444222754628 2304 98-99 0.43408132405562583 2304 100-101 0.38807014360198266 2202 1 0.10922110599263135 2202 2 0.1420631329811215 2202 3 0.08472980162589039 2202 4 0.07622034802045619 2202 5 0.09102566881768581 2202 6 0.08607010259669323 2202 7 0.12830338123446694 2202 8 0.08333488433913772 2202 9 0.009038982820207764 2202 10-11 0.1592769930904936 2202 12-13 0.15198594403305066 2202 14-15 0.18511956697352616 2202 16-17 0.16881769516484013 2202 18-19 0.29000721370261573 2202 20-21 0.23102861816933995 2202 22-23 0.2619553497691314 2202 24-25 0.24150834757713824 2202 26-27 0.12724474845656175 2202 28-29 0.22539763612937236 2202 30-31 0.26778596623001505 2202 32-33 0.2480183589704552 2202 34-35 0.2958905071893625 2202 36-37 0.32898525605630624 2202 38-39 0.23403901252078185 2202 40-41 0.37371501282024866 2202 42-43 0.2283881687672178 2202 44-45 0.2076052551784997 2202 46-47 0.2666306598433579 2202 48-49 0.18614401770189204 2202 50-51 0.20000403309638415 2202 52-53 0.3022242686572909 2202 54-55 0.448619075656822 2202 56-57 0.4427376540282353 2202 58-59 0.3339975037580132 2202 60-61 0.3317915604010935 2202 62-63 0.24036218453006342 2202 64-65 0.28859200466002477 2202 66-67 0.3525268840615965 2202 68-69 0.2868626495803568 2202 70-71 0.2720341491451066 2202 72-73 0.22092329063125504 2202 74-75 0.25562961640796544 2202 76-77 0.13533129359227658 2202 78-79 0.48081806566390384 2202 80-81 0.5646351420653488 2202 82-83 0.4815977514162384 2202 84-85 0.4979274295922096 2202 86-87 0.5174146614922464 2202 88-89 0.4577894602555972 2202 90-91 0.351605860276198 2202 92-93 0.37559123511270087 2202 94-95 0.42192967456789177 2202 96-97 0.5660878949675698 2202 98-99 0.5102276923875451 2202 100-101 0.528863708158692 1102 1 0.10262779435661429 1102 2 0.15078081246157637 1102 3 0.08751201894290261 1102 4 0.0796290556109227 1102 5 0.04178311709884497 1102 6 0.05222326341542072 1102 7 0.03234480113346905 1102 8 0.07707403713346395 1102 9 0.09945301460858502 1102 10-11 0.1481384118980671 1102 12-13 0.108269601809873 1102 14-15 0.10260087614378222 1102 16-17 0.08347809940715223 1102 18-19 0.21231033325100412 1102 20-21 0.1841820835796284 1102 22-23 0.11492299547129647 1102 24-25 0.09010962946577905 1102 26-27 0.1044207567019555 1102 28-29 0.10736870782848484 1102 30-31 0.21740331285115388 1102 32-33 0.24344183693936827 1102 34-35 0.18719769094687422 1102 36-37 0.19221346095044822 1102 38-39 0.1472957501950134 1102 40-41 0.30386581196932383 1102 42-43 0.18256286259435228 1102 44-45 0.09649772724176842 1102 46-47 0.047566154024138996 1102 48-49 0.19269069964959584 1102 50-51 0.12960560157573298 1102 52-53 0.11105931123002222 1102 54-55 0.21577062764012567 1102 56-57 0.2306033355524022 1102 58-59 0.1709305577199629 1102 60-61 0.151227675397962 1102 62-63 0.053906878984030016 1102 64-65 0.0608770913348593 1102 66-67 0.07917871520046305 1102 68-69 0.18519645511006644 1102 70-71 0.1259968402005356 1102 72-73 0.059326731726152104 1102 74-75 0.07481690336374669 1102 76-77 -0.11432384415446961 1102 78-79 0.19737049152118402 1102 80-81 0.40200782958677195 1102 82-83 0.3636331423802446 1102 84-85 0.3082888896309868 1102 86-87 0.35977234996032337 1102 88-89 0.2555647287440621 1102 90-91 0.21752637603701785 1102 92-93 0.12744855171723657 1102 94-95 0.10111664526356634 1102 96-97 0.27598980488687275 1102 98-99 0.42729248273413845 1102 100-101 0.3422071025239397 2305 1 0.07163169032186545 2305 2 0.09320531081320382 2305 3 0.04188735830447854 2305 4 0.02803543576443701 2305 5 0.03103607802095354 2305 6 -0.0655788067250711 2305 7 0.0542255075532907 2305 8 0.03812786482050967 2305 9 -0.030298994584171624 2305 10-11 0.057707518830390825 2305 12-13 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10 319.0 11 175.0 12 126.0 13 238.0 14 591.0 15 1426.0 16 2687.0 17 4772.0 18 7205.0 19 10467.0 20 14817.0 21 20173.0 22 27012.0 23 35566.0 24 47140.0 25 62093.0 26 85298.0 27 126361.0 28 208617.0 29 272603.0 30 192611.0 31 170269.0 32 224729.0 33 319265.0 34 495364.0 35 869418.0 36 1730154.0 37 2602986.0 38 609655.0 39 174.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.891494825990755 24.348625403747633 23.401206110530325 14.358673659731291 2 27.52215545389391 27.053957083678625 32.47281440702569 12.951073055401775 3 34.91666548975566 18.302902508171346 30.885685917041894 15.894746085031104 4 26.48696069971327 18.255916978442112 33.56655389447264 21.690568427371982 5 35.656403676520796 17.289248716307522 31.521103742197514 15.53324386497417 6 29.08469152819457 18.419691422581298 36.9339926876318 15.561624361592333 7 30.34541925767133 24.6058660067229 28.490690558517368 16.558024177088406 8 26.39914251847864 31.45946734560445 25.880384864586592 16.26100527133032 9 34.39309701831629 23.35641187997027 24.54580152859246 17.70468957312098 10-11 26.187277383188036 29.41687596924738 27.43255610152432 16.963290546040263 12-13 23.14206606085584 27.774835206454295 27.14061522923729 21.94248350345257 14-15 22.350291959293205 28.380680827359633 27.619780186851983 21.64924702649518 16-17 24.456318842046265 26.258713708946523 28.451257510208873 20.833709938798336 18-19 20.43229354201861 26.534149437375998 31.71252165686101 21.32103536374439 20-21 23.584539443184656 26.77525800035982 31.342879054982152 18.297323501473368 22-23 20.6606688440647 32.99103716065758 28.056204058517487 18.29208993676024 24-25 20.784262050597107 26.10731831201501 31.524763364868306 21.583656272519576 26-27 20.556788256197166 26.215117532834675 28.29475337827383 24.93334083269433 28-29 23.110787339572212 29.729343886145948 27.964091733231683 19.19577704105016 30-31 19.4657601773124 27.152287951883576 31.08541215456478 22.296539716239245 32-33 19.404541321560977 30.06817827913244 28.48542831585147 22.041852083455115 34-35 22.821849077161055 30.29226876465384 28.548955926844883 18.33692623134022 36-37 19.410181833409872 30.81604995194873 31.729819189571533 18.04394902506986 38-39 19.99262289988439 27.349410507436904 30.755814424729877 21.902152167948827 40-41 23.14300709806401 26.958347761353597 30.59106484346844 19.30758029711395 42-43 20.12797332584197 30.221108752151082 31.048283971710898 18.602633950296045 44-45 19.55171323558714 27.43096421038722 31.273838859457044 21.743483694568596 46-47 22.976353920149293 27.26185320457923 28.174879711342836 21.586913163928635 48-49 20.28134734443297 30.49630896713529 30.56118964863912 18.661154039792624 50-51 23.162377837762165 27.53814998661404 30.02794472703694 19.271527448586863 52-53 19.64235301342627 27.373391572693006 27.365758020066476 25.618497393814245 54-55 23.008442232141608 27.365743744608906 30.301497235262946 19.324316787986543 56-57 19.912504137433867 27.044251640247996 30.063778362650435 22.9794658596677 58-59 19.812171309003162 26.839051269500363 30.67015960187663 22.67861781961985 60-61 22.612418669064084 27.069560641732725 30.856768675412027 19.461252013791164 62-63 19.68070933884748 27.152687540017872 30.700880096919164 22.465723024215485 64-65 23.04272721796082 27.21829857400717 30.437288132054345 19.30168607597766 66-67 20.24113826014902 33.225912907274214 26.957025707996074 19.57592312458069 68-69 20.202824674533 33.12113889603574 27.02116660241154 19.654869827019716 70-71 19.81035562297746 34.13227185190199 27.007484969805713 19.049887555314836 72-73 19.780547881562217 34.22999999140092 26.839044573804582 19.150407553232277 74-75 20.581052523467925 33.060811652107816 26.767417131211342 19.59071869321292 76-77 20.82843216582523 31.863055755239383 27.373691746785006 19.93482033215038 78-79 20.72745244567679 31.496007046399544 27.893207965752527 19.883332542171146 80-81 20.13676366764616 31.85240656791829 27.687905723124935 20.322924041310618 82-83 19.399886825555072 31.510227917857335 28.107693191018875 20.982192065568718 84-85 19.528840624601603 31.045887618579265 28.34459916713563 21.0806725896835 86-87 19.426957674204996 31.085508141291097 28.844390399918236 20.64314378458568 88-89 19.65380623803184 31.289125570238824 28.55724840694235 20.499819784786986 90-91 20.117004066983 30.674927559552252 28.127947211518737 21.08012116194601 92-93 19.749699484693117 31.309084423990775 27.840928602895477 21.10028748842063 94-95 19.50086335041398 31.82738152470048 27.67253568827352 20.99921943661202 96-97 19.94822034542252 32.16957391525113 27.10675421224916 20.77545152707719 98-99 20.29718542329399 31.190784059030452 27.0545970154186 21.457433502256954 100-101 20.304761282327092 31.305186831179466 26.793195788343994 21.596856098149452 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 96.0 1 76.5 2 113.0 3 340.5 4 415.0 5 261.5 6 204.0 7 207.5 8 213.5 9 214.5 10 218.0 11 208.5 12 201.0 13 214.0 14 224.0 15 254.0 16 311.0 17 395.0 18 568.0 19 835.0 20 1183.0 21 1744.5 22 2602.5 23 3934.0 24 5720.5 25 8032.5 26 11543.0 27 16271.5 28 21380.5 29 27098.5 30 33751.0 31 41375.5 32 52581.0 33 77270.5 34 119500.5 35 198827.0 36 309054.5 37 428371.5 38 563371.0 39 631096.0 40 633503.0 41 590672.0 42 530153.0 43 523615.5 44 501664.5 45 460621.0 46 448869.0 47 398880.5 48 304984.5 49 245744.0 50 203255.0 51 167149.0 52 144915.0 53 120312.5 54 88830.5 55 59287.5 56 40749.5 57 29695.5 58 23085.5 59 17677.5 60 13202.0 61 9938.0 62 7220.5 63 5181.0 64 3752.0 65 2772.0 66 2017.0 67 1491.5 68 1140.5 69 760.5 70 453.5 71 300.0 72 225.0 73 153.5 74 111.0 75 85.5 76 65.0 77 50.0 78 27.5 79 22.0 80 16.5 81 10.0 82 6.5 83 3.5 84 5.0 85 4.0 86 0.5 87 0.5 88 1.0 89 1.0 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1614532189697155 2 0.0 3 0.0016087603015922062 4 0.010291153684994416 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.228061298925348E-5 22-23 2.0263011432268243E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 6.201709559573008E-4 38-39 0.0018420919483880223 40-41 0.0059499569932933115 42-43 0.002499104743313083 44-45 0.0011359567015059471 46-47 0.0013201658963447494 48-49 0.0033955894915285874 50-51 6.87714327398195E-4 52-53 0.015086733057297902 54-55 0.026747175090594082 56-57 0.028276111407756137 58-59 0.02113493495450524 60-61 0.030535744197778783 62-63 0.005655222281551228 64-65 0.011107814448779774 66-67 0.02844803998960569 68-69 0.02690068275295975 70-71 0.030320833470466844 72-73 0.030787496764058477 74-75 0.03850586202780429 76-77 0.01932354453859035 78-79 0.010874482801983956 80-81 0.015719184626244456 82-83 0.007595559133853279 84-85 0.010978868012392612 86-87 0.03333572395932857 88-89 0.015491993285943266 90-91 0.021441950279236576 92-93 0.048870699390734226 94-95 0.056042577376458264 96-97 0.03969708148776188 98-99 0.03495676487391003 100-101 0.03487694088947989 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8142916.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.40019823253088 #Duplication Level Percentage of deduplicated Percentage of total 1 88.94169029032017 29.70670086833061 2 4.894655916587808 3.2696495578812588 3 1.5699957426605544 1.573145070872762 4 0.8963413239691169 1.1975191161831078 5 0.6075899199554747 1.014681188530021 6 0.4390974222168729 0.879956456726212 7 0.2690081775377858 0.6289448519153734 8 0.25002798987192804 0.6680787540322896 9 0.18049049793098923 0.542557656898492 >10 1.5044512016512062 10.113412966611238 >50 0.1899697903369319 4.386793353319983 >100 0.19470371027236352 14.163994243242895 >500 0.03341827939525414 7.676605963170013 >1k 0.027778910411108387 16.598081264930357 >5k 5.206657965548033E-4 1.0796653806856027 >10k+ 2.6016108591844685E-4 6.5002133066699885 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 448878 5.512496997390124 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 16144 0.19825821609850822 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 13512 0.16593564271079306 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 13057 0.1603479638006827 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 11409 0.14010951359439297 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 10456 0.12840608941563442 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10186 0.12509032390853597 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9699 0.11910966538276951 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8621 0.10587116458035425 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0017684082704525013 0.0 0.0 2 0.001989459304259064 0.0 0.0 3 0.0020754235951838383 0.0 0.0 4 0.0022841940160011477 0.0 0.0 5 0.002628051179700245 0.0 0.0 6 0.002935066504431582 0.0 0.0 7 0.003524535927915749 0.0 0.0 8 0.00412628596438917 0.0 0.0 9 0.004629791096948562 0.0 0.0 10-11 0.007190298905207913 0.0 0.0 12-13 0.009971857747273827 0.0 0.0 14-15 0.013097273753038837 0.0 6.14030649462674E-6 16-17 0.015676202480782067 0.0 4.298214546238719E-5 18-19 0.019213019021687074 0.0 6.140306494626741E-5 20-21 0.023517373874420416 0.0 6.140306494626741E-5 22-23 0.027950675163540925 0.0 6.140306494626741E-5 24-25 0.03320063721644678 0.0 6.140306494626741E-5 26-27 0.03886199980449264 0.0 6.140306494626741E-5 28-29 0.044891780782216104 0.0 7.368367793552089E-5 30-31 0.0702205450725514 0.0 7.982398443014763E-5 32-33 0.07849153792081362 0.0 8.596429092477436E-5 34-35 0.08533183935582782 0.0 8.596429092477436E-5 36-37 0.0926081025519605 0.0 8.596429092477436E-5 38-39 0.10027120505725468 0.0 9.824490391402785E-5 40-41 0.1085667591314954 0.0 9.824490391402785E-5 42-43 0.11758072906560746 0.0 9.824490391402785E-5 44-45 0.12662540053219265 1.228061298925348E-5 1.1052551690328133E-4 46-47 0.13667708226389663 1.228061298925348E-5 1.1052551690328133E-4 48-49 0.1470726211592997 1.228061298925348E-5 1.1052551690328133E-4 50-51 0.1586962213536281 2.456122597850696E-5 1.1052551690328133E-4 52-53 0.17052859196877385 2.456122597850696E-5 1.1052551690328133E-4 54-55 0.1829381513944145 2.456122597850696E-5 1.1052551690328133E-4 56-57 0.1960354251474533 2.456122597850696E-5 1.1052551690328133E-4 58-59 0.21107917605928883 2.456122597850696E-5 1.1052551690328133E-4 60-61 0.2272404627531464 2.456122597850696E-5 1.1052551690328133E-4 62-63 0.24477717810180039 2.456122597850696E-5 1.1052551690328133E-4 64-65 0.2632165185051645 2.456122597850696E-5 1.1052551690328133E-4 66-67 0.2835593539218629 2.456122597850696E-5 1.1666582339790808E-4 68-69 0.30679427369753043 2.456122597850696E-5 1.2280612989253481E-4 70-71 0.33185286450210216 2.456122597850696E-5 1.2894643638716155E-4 72-73 0.3604237106216004 2.456122597850696E-5 1.4736735587104178E-4 74-75 0.3911498043207126 2.456122597850696E-5 1.4736735587104178E-4 76-77 0.42516096199445014 2.456122597850696E-5 1.4736735587104178E-4 78-79 0.46256156885322164 2.456122597850696E-5 1.5964796886029525E-4 80-81 0.5062437092559963 2.456122597850696E-5 1.5964796886029525E-4 82-83 0.5556793168442362 2.456122597850696E-5 1.5964796886029525E-4 84-85 0.608621039440908 2.456122597850696E-5 1.5964796886029525E-4 86-87 0.6673039486100556 2.456122597850696E-5 1.5964796886029525E-4 88-89 0.7336008378325405 2.456122597850696E-5 1.964898078280557E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 60775 0.0 81.884735 3 GATCGGA 61095 0.0 81.5434 1 CGGAAGA 60690 0.0 81.52737 4 ATCGGAA 61825 0.0 80.37883 2 GAAGAGC 61505 0.0 80.33828 6 GGAAGAG 64940 0.0 76.373985 5 GAGCACA 90185 0.0 54.83179 9 AGAGCAC 90100 0.0 54.82027 8 AAGAGCA 90890 0.0 54.312428 7 TTGTGTT 92835 0.0 46.925224 1 GCCGTCT 53530 0.0 45.611797 50-51 ATGCCGT 53410 0.0 45.499523 48-49 TCGTATG 54300 0.0 45.422096 44-45 TATGCCG 53755 0.0 45.331215 48-49 CGTCTTC 53795 0.0 45.28597 52-53 TCTCGTA 53965 0.0 45.272854 42-43 TGCCGTC 53610 0.0 45.23358 50-51 CGTATGC 54045 0.0 45.196266 46-47 CGTATCT 53580 0.0 45.08103 38-39 CTCGTAT 54025 0.0 45.02918 44-45 >>END_MODULE