FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_34302-1.341000000358ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_34302-1.341000000358ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8142916
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4488785.512496997390124TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC161440.19825821609850822No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA135120.16593564271079306No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG130570.1603479638006827No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA114090.14010951359439297No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC104560.12840608941563442No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC101860.12509032390853597No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA96990.11910966538276951No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG86210.10587116458035425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG607750.081.8847353
GATCGGA610950.081.54341
CGGAAGA606900.081.527374
ATCGGAA618250.080.378832
GAAGAGC615050.080.338286
GGAAGAG649400.076.3739855
GAGCACA901850.054.831799
AGAGCAC901000.054.820278
AAGAGCA908900.054.3124287
TTGTGTT928350.046.9252241
GCCGTCT535300.045.61179750-51
ATGCCGT534100.045.49952348-49
TCGTATG543000.045.42209644-45
TATGCCG537550.045.33121548-49
CGTCTTC537950.045.2859752-53
TCTCGTA539650.045.27285442-43
TGCCGTC536100.045.2335850-51
CGTATGC540450.045.19626646-47
CGTATCT535800.045.0810338-39
CTCGTAT540250.045.0291844-45
Produced by FastQC (version 0.11.2)