##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_34298-1.341000000357c1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5191010 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66724413938713 33.0 33.0 33.0 33.0 33.0 2 32.649024756261305 33.0 33.0 33.0 33.0 33.0 3 32.71358386903512 33.0 33.0 33.0 33.0 33.0 4 36.66726263289803 37.0 37.0 37.0 37.0 37.0 5 36.67252133977781 37.0 37.0 37.0 37.0 37.0 6 36.55372499764015 37.0 37.0 37.0 37.0 37.0 7 36.48639147295035 37.0 37.0 37.0 37.0 37.0 8 36.42109416086657 37.0 37.0 37.0 37.0 37.0 9 36.432856997000584 37.0 37.0 37.0 37.0 37.0 10-11 36.30885116769184 37.0 37.0 37.0 37.0 37.0 12-13 36.340767788927394 37.0 37.0 37.0 37.0 37.0 14-15 38.216415302609704 40.0 37.0 40.0 37.0 40.0 16-17 38.17091760562973 40.0 37.0 40.0 37.0 40.0 18-19 38.08893596814492 40.0 37.0 40.0 37.0 40.0 20-21 38.08514046399448 40.0 37.0 40.0 37.0 40.0 22-23 38.008634157899905 40.0 37.0 40.0 35.0 40.0 24-25 37.98048472262623 40.0 37.0 40.0 33.0 40.0 26-27 37.88049618475017 40.0 37.0 40.0 33.0 40.0 28-29 37.76137312392001 40.0 37.0 40.0 33.0 40.0 30-31 37.64746638900715 40.0 37.0 40.0 33.0 40.0 32-33 37.54375911431494 40.0 37.0 40.0 33.0 40.0 34-35 37.47108741844073 40.0 37.0 40.0 33.0 40.0 36-37 37.41145489991351 40.0 37.0 40.0 33.0 40.0 38-39 37.271005353486125 40.0 37.0 40.0 33.0 40.0 40-41 37.1064594366029 40.0 37.0 40.0 33.0 40.0 42-43 37.0410632034999 40.0 37.0 40.0 33.0 40.0 44-45 36.94106291453879 37.0 37.0 40.0 33.0 40.0 46-47 36.86741693812957 37.0 37.0 40.0 33.0 40.0 48-49 36.66815465583769 37.0 37.0 40.0 33.0 40.0 50-51 36.49813186643833 37.0 37.0 40.0 33.0 40.0 52-53 36.33505165661403 37.0 37.0 40.0 33.0 40.0 54-55 35.993623302594294 37.0 37.0 40.0 30.0 40.0 56-57 35.86280165131641 37.0 37.0 40.0 27.0 40.0 58-59 35.63873196160284 37.0 37.0 40.0 27.0 40.0 60-61 35.352206699659604 37.0 33.0 40.0 27.0 40.0 62-63 35.10721593678302 37.0 33.0 38.5 27.0 40.0 64-65 34.84600434212224 37.0 33.0 37.0 27.0 40.0 66-67 34.34961298475634 37.0 33.0 37.0 27.0 40.0 68-69 34.095653158055946 37.0 33.0 37.0 27.0 40.0 70-71 33.779533366339116 37.0 33.0 37.0 27.0 38.5 72-73 33.3370758291739 37.0 33.0 37.0 27.0 37.0 74-75 32.85879693932395 37.0 33.0 37.0 24.5 37.0 76-77 30.23002970520188 33.0 30.0 35.0 22.0 37.0 78-79 32.59251455882381 35.0 33.0 37.0 27.0 37.0 80-81 32.975432333977395 37.0 33.0 37.0 27.0 37.0 82-83 32.89172935517365 37.0 33.0 37.0 27.0 37.0 84-85 32.7854176740172 37.0 33.0 37.0 27.0 37.0 86-87 32.67792953587067 37.0 33.0 37.0 27.0 37.0 88-89 32.52244659517127 37.0 33.0 37.0 22.0 37.0 90-91 32.403440370949006 33.0 33.0 37.0 22.0 37.0 92-93 32.20607357720367 33.0 33.0 37.0 22.0 37.0 94-95 32.08588078235257 33.0 33.0 37.0 22.0 37.0 96-97 31.920746155372463 33.0 33.0 37.0 22.0 37.0 98-99 31.78720451318722 33.0 33.0 37.0 22.0 37.0 100-101 30.191501172218892 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1144378247278297 2306 2 0.1933303495851959 2306 3 0.15815653340096247 2306 4 0.1709194106029983 2306 5 0.16685337017632662 2306 6 0.18828022365804742 2306 7 0.19030368789407248 2306 8 0.1921669037583058 2306 9 0.17882037106106452 2306 10-11 0.21872261526451808 2306 12-13 0.2644025208590506 2306 14-15 0.3934494112304563 2306 16-17 0.5071204985691722 2306 18-19 0.5842386718093664 2306 20-21 0.5465675267141918 2306 22-23 0.4778372260682957 2306 24-25 0.4685402795122613 2306 26-27 0.49343394575573285 2306 28-29 0.48264293451749296 2306 30-31 0.602043285740038 2306 32-33 0.531462457852875 2306 34-35 0.508523712890522 2306 36-37 0.4907140885934851 2306 38-39 0.5945309588233769 2306 40-41 0.6125336224985034 2306 42-43 0.5597141983258993 2306 44-45 0.5372606450365396 2306 46-47 0.4928597783957258 2306 48-49 0.5119539089217753 2306 50-51 0.6054472066889716 2306 52-53 0.5386101487189308 2306 54-55 0.7231472901424851 2306 56-57 0.6089815105083716 2306 58-59 0.4899710537894393 2306 60-61 0.4872780277936073 2306 62-63 0.4307305068360989 2306 64-65 0.4093035282352844 2306 66-67 0.531710510559904 2306 68-69 0.4711868843773104 2306 70-71 0.43275079801774297 2306 72-73 0.30275403752820296 2306 74-75 0.4602492083516978 2306 76-77 0.14841339283157495 2306 78-79 0.5373109744158597 2306 80-81 0.5482865246525321 2306 82-83 0.5181845423156162 2306 84-85 0.5771874691899583 2306 86-87 0.6095110826056995 2306 88-89 0.49892481578153536 2306 90-91 0.5264459068458365 2306 92-93 0.46743212811021095 2306 94-95 0.5444806144255736 2306 96-97 0.5274004187800863 2306 98-99 0.42865655016484894 2306 100-101 0.3910071965811497 2307 1 0.10084089422277032 2307 2 0.19070091496971742 2307 3 0.12349560153348449 2307 4 0.1416013533428071 2307 5 0.13325103872259092 2307 6 0.09806363572606358 2307 7 0.1693508896285465 2307 8 0.1903386862498735 2307 9 0.1299407603539251 2307 10-11 0.17696217375203815 2307 12-13 0.20830598595719607 2307 14-15 0.28279985729719925 2307 16-17 0.3983024186341666 2307 18-19 0.47292958822902875 2307 20-21 0.37679419890965704 2307 22-23 0.38291283934975695 2307 24-25 0.40558111444730827 2307 26-27 0.37117265369636243 2307 28-29 0.37387294811272653 2307 30-31 0.5641329800834995 2307 32-33 0.5301280206900998 2307 34-35 0.454524568503075 2307 36-37 0.4683040714378137 2307 38-39 0.5429853081429385 2307 40-41 0.609938803163729 2307 42-43 0.5916188733909351 2307 44-45 0.47228854712377455 2307 46-47 0.45381331962337157 2307 48-49 0.4983484753599541 2307 50-51 0.40140001701556827 2307 52-53 0.5962771258376236 2307 54-55 0.6749225052637939 2307 56-57 0.6245296513180278 2307 58-59 0.5570494975636109 2307 60-61 0.3867180442159608 2307 62-63 0.37986727168955525 2307 64-65 0.3805334959984492 2307 66-67 0.43734084276045593 2307 68-69 0.4807363297126628 2307 70-71 0.5098846491570797 2307 72-73 0.4274178351067235 2307 74-75 0.40916992922758055 2307 76-77 0.24418341156000878 2307 78-79 0.5616744331747086 2307 80-81 0.6153988600430012 2307 82-83 0.585403226220933 2307 84-85 0.673952828002065 2307 86-87 0.7119190336995018 2307 88-89 0.7023747410993693 2307 90-91 0.6873527777498936 2307 92-93 0.5117720412415352 2307 94-95 0.5607399665818988 2307 96-97 0.7415155894876939 2307 98-99 0.6336202306544685 2307 100-101 0.41694423343592746 2201 1 0.1339494322089081 2201 2 0.20113431945141969 2201 3 0.12216789902083036 2201 4 0.12738903543494473 2201 5 0.1084469639646315 2201 6 0.06160181828937539 2201 7 0.11932641774113506 2201 8 0.17183779611010408 2201 9 0.2099905823948447 2201 10-11 0.18307901609120592 2201 12-13 0.176350185993158 2201 14-15 0.21611912955732038 2201 16-17 0.24835738189272405 2201 18-19 0.3214316624301645 2201 20-21 0.3056808721142872 2201 22-23 0.27255195518374364 2201 24-25 0.22373507295072415 2201 26-27 0.2465757042770491 2201 28-29 0.31240749005922197 2201 30-31 0.3398070699719895 2201 32-33 0.4023122866467048 2201 34-35 0.37025369713472855 2201 36-37 0.37946321352942647 2201 38-39 0.29485006706578787 2201 40-41 0.44652654213930987 2201 42-43 0.3741010407048151 2201 44-45 0.36504867573852096 2201 46-47 0.354133864708146 2201 48-49 0.4269902368465708 2201 50-51 0.43749490766124666 2201 52-53 0.3968309403803332 2201 54-55 0.6557017564973222 2201 56-57 0.5333718844787185 2201 58-59 0.3340196084714293 2201 60-61 0.30963741172993053 2201 62-63 0.42107718075705236 2201 64-65 0.3883025419893329 2201 66-67 0.4492663904797567 2201 68-69 0.45814271406802476 2201 70-71 0.24057074302564985 2201 72-73 0.3288168457735807 2201 74-75 0.27340977146587164 2201 76-77 0.1920845732349683 2201 78-79 0.45839014775189213 2201 80-81 0.40140847237337596 2201 82-83 0.4982282363300854 2201 84-85 0.4995792386205622 2201 86-87 0.4890039969079183 2201 88-89 0.4612152414201205 2201 90-91 0.5124223379121418 2201 92-93 0.4086778723278215 2201 94-95 0.429348934692527 2201 96-97 0.5284646545172329 2201 98-99 0.4051308764351482 2201 100-101 0.3350775466253424 1101 1 0.11002414887509104 1101 2 0.1463685625738691 1101 3 0.10958974300717017 1101 4 0.1088546808444022 1101 5 0.0863763309040948 1101 6 0.1033210803126039 1101 7 0.12142250598849103 1101 8 0.15406206504862752 1101 9 0.1446871507733789 1101 10-11 0.14683170431244008 1101 12-13 0.10666557006506139 1101 14-15 0.2376797439585019 1101 16-17 0.25417496965796715 1101 18-19 0.3167022991503927 1101 20-21 0.33979875179074526 1101 22-23 0.23063822122603028 1101 24-25 0.2929810596162312 1101 26-27 0.2685570052871782 1101 28-29 0.3133058426389752 1101 30-31 0.39532496967510156 1101 32-33 0.32842001677903454 1101 34-35 0.4022895682098948 1101 36-37 0.36780821467053926 1101 38-39 0.31223283876355623 1101 40-41 0.5028068013064484 1101 42-43 0.40025619433814796 1101 44-45 0.394246888182586 1101 46-47 0.35830820020730414 1101 48-49 0.19238955746553188 1101 50-51 0.22304204931518967 1101 52-53 0.17295783306344248 1101 54-55 0.47254139441151466 1101 56-57 0.4512461730626711 1101 58-59 0.34971734642633834 1101 60-61 0.37126526787763225 1101 62-63 0.36503808000421856 1101 64-65 0.18758079493399293 1101 66-67 0.41882703436179725 1101 68-69 0.3556985739201437 1101 70-71 0.3314115520410965 1101 72-73 -0.05047232525040357 1101 74-75 0.11080559098005693 1101 76-77 -0.2088271473841914 1101 78-79 0.19193833546763273 1101 80-81 0.4582097292740528 1101 82-83 0.4514568465775284 1101 84-85 0.30249105683149224 1101 86-87 0.2806200759653876 1101 88-89 0.214515546038605 1101 90-91 0.12835216129644778 1101 92-93 0.156639551292578 1101 94-95 0.28318172440919653 1101 96-97 0.39206564344717876 1101 98-99 0.16632150111906085 1101 100-101 0.23790745382675027 2304 1 0.125019719803511 2304 2 0.19939650087619754 2304 3 0.10750878239075234 2304 4 0.09633376578415209 2304 5 0.09159279298983591 2304 6 0.09672527879125425 2304 7 0.13469566019048784 2304 8 0.15724677842442247 2304 9 0.08918877476629206 2304 10-11 0.07847847530835139 2304 12-13 0.13102124560393236 2304 14-15 0.1813503897593236 2304 16-17 0.21376355811988645 2304 18-19 0.33691855032795814 2304 20-21 0.28280011566358354 2304 22-23 0.23838979952216732 2304 24-25 0.17589187358115765 2304 26-27 0.133293159165774 2304 28-29 0.3523379147414474 2304 30-31 0.3709770274358135 2304 32-33 0.2702663069043112 2304 34-35 0.2707946510968071 2304 36-37 0.3289005783887049 2304 38-39 0.3280432182576618 2304 40-41 0.5018781023286536 2304 42-43 0.31461411956416896 2304 44-45 0.3101964670692752 2304 46-47 0.2845599401997134 2304 48-49 0.31766830776431476 2304 50-51 0.28346091294196896 2304 52-53 0.24019324876344683 2304 54-55 0.48912088570101275 2304 56-57 0.5030452197577375 2304 58-59 0.354822027181676 2304 60-61 0.4000689967340847 2304 62-63 0.3111868968093887 2304 64-65 0.26431340555532756 2304 66-67 0.21120340702247375 2304 68-69 0.1282411422192382 2304 70-71 0.23624697336532563 2304 72-73 -0.003730856485901768 2304 74-75 0.11504484520876446 2304 76-77 -0.02534477227698062 2304 78-79 0.22084822143661142 2304 80-81 0.2963426295426075 2304 82-83 0.2373611505102602 2304 84-85 0.3033360955682909 2304 86-87 0.3372074411599044 2304 88-89 0.33756921513774074 2304 90-91 0.34408098963125866 2304 92-93 0.08728099334204131 2304 94-95 0.22546318839311397 2304 96-97 0.3372203797416624 2304 98-99 0.28868620352762875 2304 100-101 0.3046345808356534 2202 1 0.06805117047827025 2202 2 0.13476559898415275 2202 3 0.08591872099177777 2202 4 0.07932671820385195 2202 5 0.09042767303490962 2202 6 0.09102305079603212 2202 7 0.10746179633242292 2202 8 0.0419818029683654 2202 9 0.1024714552464232 2202 10-11 0.1698853570018528 2202 12-13 0.1259748703346233 2202 14-15 0.14081676116837372 2202 16-17 0.12502586269429372 2202 18-19 0.2351563944256796 2202 20-21 0.23628830275049495 2202 22-23 0.20643050960109832 2202 24-25 0.21281511703306677 2202 26-27 0.15319070581491445 2202 28-29 0.21082578883441982 2202 30-31 0.21284101070106942 2202 32-33 0.23644052976643337 2202 34-35 0.23918813143296802 2202 36-37 0.26502673059766124 2202 38-39 0.2377586806801304 2202 40-41 0.2470289132437955 2202 42-43 0.20682474362192949 2202 44-45 0.2471661379561425 2202 46-47 0.2121206219005174 2202 48-49 0.2030653144989074 2202 50-51 0.23098839120194015 2202 52-53 0.26432795092362227 2202 54-55 0.4445368546520143 2202 56-57 0.44402147395055636 2202 58-59 0.35573727363831864 2202 60-61 0.3138449035036288 2202 62-63 0.2644906782978822 2202 64-65 0.2299383240464863 2202 66-67 0.2798321544728779 2202 68-69 0.3022441260948341 2202 70-71 0.269295221381725 2202 72-73 0.31866490348947707 2202 74-75 0.22025854182373195 2202 76-77 0.08104056500007673 2202 78-79 0.36113549771115316 2202 80-81 0.3613919000058914 2202 82-83 0.41062246915375056 2202 84-85 0.33018575936062433 2202 86-87 0.3792247914575171 2202 88-89 0.39241879546502645 2202 90-91 0.31051421498167997 2202 92-93 0.2503431435061074 2202 94-95 0.29502035412862426 2202 96-97 0.4961916990632993 2202 98-99 0.40366061627274163 2202 100-101 0.4874761224351545 1102 1 0.08539657755297725 1102 2 0.12848354970846998 1102 3 0.07095092676443215 1102 4 0.0478199653595226 1102 5 0.05439799468835105 1102 6 0.047587805330536526 1102 7 -0.0034678217628041352 1102 8 0.04570219243716167 1102 9 0.06377741595525066 1102 10-11 0.10752571522342436 1102 12-13 0.07455893137887415 1102 14-15 0.06498892479117302 1102 16-17 0.049319555996738984 1102 18-19 0.08890899618783266 1102 20-21 0.17602596492048406 1102 22-23 -0.030419913853627634 1102 24-25 0.020285451075856997 1102 26-27 0.040093849187002206 1102 28-29 0.11420097206628554 1102 30-31 0.12096375779675128 1102 32-33 0.10669810095951249 1102 34-35 0.12509401415413635 1102 36-37 0.08870020683881563 1102 38-39 0.016014290444495316 1102 40-41 0.12278441867684364 1102 42-43 -0.03911464901332096 1102 44-45 0.024494503162692638 1102 46-47 -0.07338193878117494 1102 48-49 -0.01701189393419611 1102 50-51 0.08659254776150505 1102 52-53 -0.005076124909585644 1102 54-55 0.09199376255876501 1102 56-57 0.05829336200827129 1102 58-59 -0.09792478810495453 1102 60-61 -0.05265127927440716 1102 62-63 -0.014708803827552686 1102 64-65 -0.15886201907311914 1102 66-67 -0.03721750592869455 1102 68-69 -0.10217950533914433 1102 70-71 -0.21206778247331926 1102 72-73 -0.27752995415044523 1102 74-75 -0.20001619513093516 1102 76-77 -0.41533946442135417 1102 78-79 -0.01028235181179582 1102 80-81 -0.024218676189875055 1102 82-83 -0.006282692377212129 1102 84-85 -0.028603925663141183 1102 86-87 0.05019178298581295 1102 88-89 -0.1583171967518524 1102 90-91 -0.12847739678063164 1102 92-93 -0.18508498330339052 1102 94-95 -0.22133219377248992 1102 96-97 0.018231526238484008 1102 98-99 0.13246937762529498 1102 100-101 0.07966775493331824 2305 1 0.09241597088473696 2305 2 0.1336243061761948 2305 3 0.07346355749494649 2305 4 0.07589999602713249 2305 5 0.032827459184694874 2305 6 -0.006151775545461646 2305 7 0.05056541131103387 2305 8 0.04287379344368958 2305 9 0.01971729957560342 2305 10-11 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-1.5513877832248255 1213 94-95 -1.51605505694679 1213 96-97 -1.762519634648033 1213 98-99 -1.871233858621732 1213 100-101 -1.7379302279711943 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 8.0 8 75.0 9 238.0 10 180.0 11 102.0 12 128.0 13 303.0 14 747.0 15 2176.0 16 4310.0 17 6689.0 18 8533.0 19 10197.0 20 12448.0 21 15074.0 22 18910.0 23 23478.0 24 29456.0 25 37233.0 26 47140.0 27 62599.0 28 89533.0 29 119406.0 30 115292.0 31 123138.0 32 161423.0 33 227179.0 34 346966.0 35 599968.0 36 1160445.0 37 1640643.0 38 326947.0 39 46.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.50236890925982 24.957797623469325 24.276346699107524 14.263486768163325 2 30.906470995047208 23.270018743943858 32.694446745431044 13.129063515577894 3 36.8574073307063 17.990157785588448 28.64619945847593 16.506235425229317 4 29.906455268494607 17.660638040034232 33.79087163406389 18.642035057407266 5 31.35721179500714 16.576196154505578 35.6512509126355 16.415341137851787 6 26.83263950560681 17.465406539382506 39.48212390267019 16.21983005234049 7 33.16067586076698 20.06439979888307 29.25328596939709 17.521638370952857 8 29.759757735007252 28.554327577870204 24.507407999599305 17.17850668752324 9 30.42650659505568 25.212473102536887 25.420139818648007 18.940880483759422 10-11 25.01361970021248 27.313027715223047 29.347756987561187 18.32559559700328 12-13 24.00803504520315 26.16340558003163 28.795725687293995 21.032833687471225 14-15 23.240669927432233 26.857904338462074 29.58265347206035 20.318772262045343 16-17 23.1822034632952 26.80526525666489 30.593718756080225 19.41881252395969 18-19 20.97507036202974 27.07527051575705 31.770146850034962 20.179512272178247 20-21 22.39901190674335 27.295960630382382 31.131641484659628 19.173385978214636 22-23 21.07538651452666 29.78322321984478 30.128628197066927 19.012762068561635 24-25 21.589218668428686 26.671399207476004 31.510091485086715 20.229290639008592 26-27 21.293052797047203 26.720628548201603 30.263975603976878 21.72234305077432 28-29 21.954725573635958 28.365433701726637 29.785802762853468 19.89403796178393 30-31 20.235744103748594 27.788378369527315 30.686956873517868 21.288920653206215 32-33 19.9303122128449 29.241217027129597 30.19235177739977 20.63611898262573 34-35 21.53397893666165 28.039119554768728 30.341330492524577 20.085571016045048 36-37 19.92950456605017 28.888959096215945 31.679552654323384 19.501983683410504 38-39 20.494440291894307 29.841290175922573 29.49440561596018 20.169863916222944 40-41 20.515951842491123 27.899363421276867 32.118684181780665 19.46600055445134 42-43 20.384992896188024 29.3423266790281 31.36564644688998 18.9070339778939 44-45 20.02503203653745 28.49280131490433 31.49391980039099 19.988246848167233 46-47 21.677903858390383 28.003348907241783 30.298999392596382 20.019747841771455 48-49 20.924226670958845 30.106310408128785 30.17167558911983 18.79778733179254 50-51 21.59274740209797 29.243142053962 29.753249273858874 19.410861270081156 52-53 19.95965283053476 28.786918353675183 29.162366096277932 22.091062719512127 54-55 21.728155289293966 28.682883806943188 30.02602420633686 19.562936697425993 56-57 20.56926370263916 28.42209248572067 29.576482712280374 21.432161099359803 58-59 20.386084368875096 28.274230444041436 30.29736163253226 21.042323554551206 60-61 21.20930281853665 28.36450106418956 30.492234643922846 19.93396147335094 62-63 20.18068270733734 28.326046103787704 30.047836501482507 21.445434687392446 64-65 21.65728093042334 28.17458692822332 29.814543606032007 20.353588535321332 66-67 20.969279471177728 30.823987849297062 28.18178419707201 20.024948482453205 68-69 21.46479517000631 30.742988053735452 28.04075172287811 19.751465053380123 70-71 20.74158220326041 31.550142574904218 28.275674683524183 19.432600538311195 72-73 20.282411631226147 31.678850374192425 28.293915134875093 19.74482285970634 74-75 21.358938343330955 30.764593727346845 27.80651975352788 20.06994817579432 76-77 21.757089791973165 29.53451935600131 28.432954311283886 20.275436540741634 78-79 21.54827382383586 29.405693013606577 29.157794572783153 19.888238589774414 80-81 20.539191418068864 30.078511059532744 29.03383238341088 20.34846513898751 82-83 19.599268833809962 29.85313677847622 29.218519897075723 21.329074490638096 84-85 19.43279800155132 29.272587645021815 29.897459283688853 21.397155069738012 86-87 19.712637492714478 29.299340104344974 30.488774874959763 20.499247527980792 88-89 19.79059462859857 29.870750923981817 30.091022986230225 20.247631461189393 90-91 20.240458932718415 29.6268257330269 29.389391925709084 20.743323408545596 92-93 19.597690676330703 30.347432578794553 29.24211114870533 20.81276559616941 94-95 19.436644775766123 30.72764058124432 29.3141783857659 20.521536257223655 96-97 20.194326519005827 31.08835974823544 28.5052377716992 20.212075961059536 98-99 20.48469275177038 30.35844261759601 28.286270006434588 20.870594624199022 100-101 20.246457668446595 30.304305103304635 28.36516920731208 21.084068020936694 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4381.0 1 2641.5 2 1045.5 3 1686.0 4 1755.5 5 1142.0 6 1027.5 7 1120.5 8 1156.0 9 1137.5 10 1059.5 11 971.5 12 909.0 13 897.0 14 921.5 15 949.5 16 984.0 17 1023.5 18 1072.5 19 1253.0 20 1491.5 21 1775.0 22 2209.0 23 2746.5 24 3498.5 25 4527.5 26 5939.5 27 7812.0 28 10104.5 29 12575.0 30 15713.5 31 20700.5 32 31969.5 33 52910.0 34 81752.0 35 122320.0 36 175828.5 37 263588.0 38 370012.0 39 429438.5 40 427343.0 41 375468.5 42 329086.5 43 320725.0 44 312215.0 45 301330.5 46 297265.0 47 264506.0 48 207199.0 49 165439.5 50 136868.0 51 113375.5 52 91382.5 53 65448.0 54 42889.5 55 29672.5 56 20464.5 57 14017.0 58 9978.5 59 6956.0 60 4935.0 61 3708.5 62 2654.5 63 1756.0 64 1224.5 65 903.0 66 652.0 67 466.5 68 335.0 69 237.0 70 161.0 71 129.0 72 104.0 73 69.5 74 41.5 75 36.0 76 35.0 77 21.5 78 17.0 79 14.5 80 7.0 81 5.5 82 2.5 83 0.5 84 2.0 85 2.5 86 1.0 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15883228889946274 2 0.0 3 0.002022727754329119 4 0.01063376876561594 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.8528147701507025E-5 22-23 3.8528147701507025E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 6.838746217017498E-4 38-39 0.0017337666465678165 40-41 0.005644373638270779 42-43 0.002600649969851724 44-45 0.0011943725787467178 46-47 0.0012136366525974714 48-49 0.003332684776180358 50-51 8.187231386570243E-4 52-53 0.015546107597558086 54-55 0.027326088757293858 56-57 0.029223600031593078 58-59 0.021845459746754486 60-61 0.031111479268966926 62-63 0.005942966782957459 64-65 0.011529548199675978 66-67 0.029204335957742324 68-69 0.027701738197383554 70-71 0.031448600561355115 72-73 0.03148712870905662 74-75 0.039019381584701245 76-77 0.019851628103201496 78-79 0.011134634685735532 80-81 0.01636483073621511 82-83 0.007667101392599899 84-85 0.011250219128840052 86-87 0.034299683491266636 88-89 0.016133661850006066 90-91 0.02200920437448589 92-93 0.04940271739025738 94-95 0.05651116064118543 96-97 0.040733884157418306 98-99 0.03596602587935681 100-101 0.03550368810693873 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5191010.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.27470471966337 #Duplication Level Percentage of deduplicated Percentage of total 1 85.40435892881953 27.56400466199796 2 5.969237224757077 3.853107376613149 3 2.4528638984709574 2.3749637412201743 4 1.2354693880724532 1.5949763876088645 5 0.8821932029900498 1.4236262566098952 6 0.5466394634793713 1.058557636314715 7 0.3788028376279539 0.855802481198896 8 0.3001708648507297 0.7750340822802624 9 0.24247352976556727 0.704318541796637 >10 1.9923454319712783 12.605150203857624 >50 0.26348611695851065 5.9611624282272135 >100 0.26756755031296525 18.226131702298108 >500 0.04631391484739913 10.319270584264578 >1k 0.017599394594752286 9.229842970250502 >5k 2.3912793725070313E-4 0.5418059344130916 >10k+ 2.391245442470218E-4 2.912245011048222 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 112450 2.1662451045172326 TruSeq Adapter, Index 2 (97% over 37bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14114 0.2718931383295351 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 12549 0.24174486275310583 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 11826 0.22781693735901107 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 8893 0.171315408754751 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 7979 0.1537080452551623 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5961 0.11483314422434171 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT 5248 0.10109785956875444 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0015218618342095276 0.0 0.0 2 0.0016374462773140488 0.0 0.0 3 0.0018300870158215838 0.0 0.0 4 0.0019264073850753514 0.0 0.0 5 0.002099784049732133 0.0 0.0 6 0.002350217009791929 0.0 0.0 7 0.002812554782210013 0.0 1.9264073850753513E-5 8 0.0033904769977326187 0.0 1.9264073850753513E-5 9 0.0037564944008969354 0.0 1.9264073850753513E-5 10-11 0.006193399743017254 0.0 1.9264073850753513E-5 12-13 0.009169699152958673 0.0 1.9264073850753513E-5 14-15 0.013022513923109377 0.0 1.9264073850753513E-5 16-17 0.017867428496573884 0.0 1.9264073850753513E-5 18-19 0.023887451574934358 0.0 1.9264073850753513E-5 20-21 0.02875163022224962 0.0 1.9264073850753513E-5 22-23 0.03449232422977417 0.0 1.9264073850753513E-5 24-25 0.04161039951762759 0.0 1.9264073850753513E-5 26-27 0.049865055162675476 0.0 1.9264073850753513E-5 28-29 0.05835087969393239 0.0 1.9264073850753513E-5 30-31 0.0874781593562717 0.0 1.9264073850753513E-5 32-33 0.09894028329747004 0.0 1.9264073850753513E-5 34-35 0.10881312114598123 0.0 1.9264073850753513E-5 36-37 0.11994775583171675 0.0 1.9264073850753513E-5 38-39 0.1316988408806764 0.0 1.9264073850753513E-5 40-41 0.14432644128984534 0.0 1.9264073850753513E-5 42-43 0.15849516760707455 0.0 1.9264073850753513E-5 44-45 0.17265426188737837 0.0 1.9264073850753513E-5 46-47 0.18782472004484674 0.0 1.9264073850753513E-5 48-49 0.20369831689786766 0.0 1.9264073850753513E-5 50-51 0.22040026892647097 0.0 3.8528147701507025E-5 52-53 0.23723706947202955 0.0 3.8528147701507025E-5 54-55 0.25470958445466296 0.0 3.8528147701507025E-5 56-57 0.27345352831144615 0.0 3.8528147701507025E-5 58-59 0.2938349184455433 0.0 3.8528147701507025E-5 60-61 0.31613308392779055 0.0 3.8528147701507025E-5 62-63 0.33898990755170955 0.0 3.8528147701507025E-5 64-65 0.36327227264058437 0.0 3.8528147701507025E-5 66-67 0.38981816640692274 0.0 4.816018462688378E-5 68-69 0.4185120044076201 0.0 5.7792221552260545E-5 70-71 0.4481786781377805 0.0 5.7792221552260545E-5 72-73 0.47943463796062813 0.0 5.7792221552260545E-5 74-75 0.5121065072115061 0.0 5.7792221552260545E-5 76-77 0.5480340049431613 0.0 5.7792221552260545E-5 78-79 0.5863309837584594 0.0 6.74242584776373E-5 80-81 0.6278546949437585 0.0 7.705629540301405E-5 82-83 0.6740981042224923 0.0 7.705629540301405E-5 84-85 0.7222293927386001 0.0 7.705629540301405E-5 86-87 0.774280920283336 0.0 7.705629540301405E-5 88-89 0.8288078813178938 0.0 7.705629540301405E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 18225 0.0 66.06219 3 CGGAAGA 18135 0.0 65.99656 4 GATCGGA 18535 0.0 65.057556 1 GAAGAGC 18390 0.0 64.971245 6 ATCGGAA 18875 0.0 63.686543 2 GGAAGAG 19695 0.0 60.93145 5 GGTCCGG 75 2.3592293E-9 50.738907 1 CGAGGCG 185 0.0 48.85334 1 CGGAGCG 60 1.478722E-6 47.567726 1 CGGTCGC 40 5.352215E-4 47.567722 1 GTCGCCG 65 2.5658683E-6 43.908672 1 GCCGTCT 13730 0.0 42.921345 50-51 CGTCTTC 13765 0.0 42.88144 52-53 ATGCCGT 13825 0.0 42.484863 48-49 TATGCCG 13840 0.0 42.438816 48-49 TGCCGTC 13825 0.0 42.35151 50-51 ACGGCGA 90 1.1830707E-8 42.28242 1 TCGTATG 14110 0.0 42.27261 44-45 CGTATGC 13965 0.0 42.240128 46-47 CTGCTTG 14250 0.0 42.107212 58-59 >>END_MODULE