FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_34298-1.341000000357c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_34298-1.341000000357c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5191010
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1124502.1662451045172326TruSeq Adapter, Index 2 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141140.2718931383295351No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC125490.24174486275310583No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG118260.22781693735901107No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA88930.171315408754751No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA79790.1537080452551623No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA59610.11483314422434171No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT52480.10109785956875444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG182250.066.062193
CGGAAGA181350.065.996564
GATCGGA185350.065.0575561
GAAGAGC183900.064.9712456
ATCGGAA188750.063.6865432
GGAAGAG196950.060.931455
GGTCCGG752.3592293E-950.7389071
CGAGGCG1850.048.853341
CGGAGCG601.478722E-647.5677261
CGGTCGC405.352215E-447.5677221
GTCGCCG652.5658683E-643.9086721
GCCGTCT137300.042.92134550-51
CGTCTTC137650.042.8814452-53
ATGCCGT138250.042.48486348-49
TATGCCG138400.042.43881648-49
TGCCGTC138250.042.3515150-51
ACGGCGA901.1830707E-842.282421
TCGTATG141100.042.2726144-45
CGTATGC139650.042.24012846-47
CTGCTTG142500.042.10721258-59
Produced by FastQC (version 0.11.2)