##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_34274-2.3410000003583d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4492544 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68755319925637 33.0 33.0 33.0 33.0 33.0 2 32.67461420522537 33.0 33.0 33.0 33.0 33.0 3 32.73351179198245 33.0 33.0 33.0 33.0 33.0 4 36.69279455025927 37.0 37.0 37.0 37.0 37.0 5 36.69884702297852 37.0 37.0 37.0 37.0 37.0 6 36.58212206714058 37.0 37.0 37.0 37.0 37.0 7 36.534291261254204 37.0 37.0 37.0 37.0 37.0 8 36.4864154919796 37.0 37.0 37.0 37.0 37.0 9 36.48641727270785 37.0 37.0 37.0 37.0 37.0 10-11 36.3662798850718 37.0 37.0 37.0 37.0 37.0 12-13 36.41105841144794 37.0 37.0 37.0 37.0 37.0 14-15 38.372792342156245 40.0 37.0 40.0 37.0 40.0 16-17 38.325932367050825 40.0 37.0 40.0 37.0 40.0 18-19 38.24625056538122 40.0 37.0 40.0 37.0 40.0 20-21 38.22664597163656 40.0 37.0 40.0 37.0 40.0 22-23 38.164430220382926 40.0 37.0 40.0 37.0 40.0 24-25 38.14144113001453 40.0 37.0 40.0 37.0 40.0 26-27 38.07720146981309 40.0 37.0 40.0 37.0 40.0 28-29 37.955997092070774 40.0 37.0 40.0 33.0 40.0 30-31 37.853993305352155 40.0 37.0 40.0 33.0 40.0 32-33 37.76938422862414 40.0 37.0 40.0 33.0 40.0 34-35 37.68664046473445 40.0 37.0 40.0 33.0 40.0 36-37 37.61647498611032 40.0 37.0 40.0 33.0 40.0 38-39 37.536558796085245 40.0 37.0 40.0 33.0 40.0 40-41 37.40125338783549 40.0 37.0 40.0 33.0 40.0 42-43 37.346093883554616 40.0 37.0 40.0 33.0 40.0 44-45 37.243114369052364 40.0 37.0 40.0 33.0 40.0 46-47 37.16448876182403 40.0 37.0 40.0 33.0 40.0 48-49 37.02425040244458 38.5 37.0 40.0 33.0 40.0 50-51 36.88312223541939 37.0 37.0 40.0 33.0 40.0 52-53 36.71658107299561 37.0 37.0 40.0 33.0 40.0 54-55 36.39869826539262 37.0 37.0 40.0 33.0 40.0 56-57 36.27654587245 37.0 37.0 40.0 33.0 40.0 58-59 36.14145348381675 37.0 37.0 40.0 33.0 40.0 60-61 35.85220122496296 37.0 37.0 40.0 30.0 40.0 62-63 35.611502747663685 37.0 35.0 40.0 27.0 40.0 64-65 35.39131681292382 37.0 33.0 40.0 27.0 40.0 66-67 34.974625512849734 37.0 33.0 37.0 27.0 40.0 68-69 34.74901592505271 37.0 33.0 37.0 27.0 40.0 70-71 34.49316178094193 37.0 33.0 37.0 27.0 40.0 72-73 34.181091159040406 37.0 33.0 37.0 27.0 40.0 74-75 33.81282620715568 37.0 33.0 37.0 27.0 37.0 76-77 31.12535903933273 33.0 30.0 35.0 24.5 37.0 78-79 33.51025766247365 35.0 33.0 37.0 27.0 37.0 80-81 33.85977733328822 37.0 33.0 37.0 27.0 37.0 82-83 33.75546316296513 37.0 33.0 37.0 27.0 37.0 84-85 33.6306492490669 37.0 33.0 37.0 27.0 37.0 86-87 33.504295227826375 37.0 33.0 37.0 27.0 37.0 88-89 33.335412741644824 37.0 33.0 37.0 27.0 37.0 90-91 33.22590218370705 37.0 33.0 37.0 27.0 37.0 92-93 33.062543071364466 37.0 33.0 37.0 27.0 37.0 94-95 32.935296682681354 35.0 33.0 37.0 27.0 37.0 96-97 32.781076935473536 33.0 33.0 37.0 27.0 37.0 98-99 32.6576052454912 33.0 33.0 37.0 27.0 37.0 100-101 31.046390196734855 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.12321550512991308 2306 2 0.19438620305106724 2306 3 0.13347992475186743 2306 4 0.14791820451399218 2306 5 0.1075837666211612 2306 6 0.18065140892147724 2306 7 0.1638669279624665 2306 8 0.13786478023099136 2306 9 0.18136851280144128 2306 10-11 0.24505694086519725 2306 12-13 0.24624952928480326 2306 14-15 0.38003833518945385 2306 16-17 0.4065310673643765 2306 18-19 0.4651400588904977 2306 20-21 0.48954745828048374 2306 22-23 0.4140392390176899 2306 24-25 0.3828030097366124 2306 26-27 0.4232190418604631 2306 28-29 0.39867796783876486 2306 30-31 0.46252177276252127 2306 32-33 0.39954066074110983 2306 34-35 0.4998123575875226 2306 36-37 0.4272551067583734 2306 38-39 0.4298456453437609 2306 40-41 0.4819831129315304 2306 42-43 0.4820931728044755 2306 44-45 0.45006700927304877 2306 46-47 0.4777262611098507 2306 48-49 0.4702296802461916 2306 50-51 0.4607783317249883 2306 52-53 0.49372395522050994 2306 54-55 0.6430731769149318 2306 56-57 0.6024364438618619 2306 58-59 0.39863450057991656 2306 60-61 0.5310807832323761 2306 62-63 0.5053346780376415 2306 64-65 0.48576271538237137 2306 66-67 0.4433933503121281 2306 68-69 0.4993910497754328 2306 70-71 0.419795573953067 2306 72-73 0.4793519243082329 2306 74-75 0.5330333390096342 2306 76-77 0.14293187164606636 2306 78-79 0.3617541415789489 2306 80-81 0.5083476365692334 2306 82-83 0.47480543127310426 2306 84-85 0.4001531017787343 2306 86-87 0.6366522128620673 2306 88-89 0.6072546530105498 2306 90-91 0.4806523210730944 2306 92-93 0.41628124594463856 2306 94-95 0.41390769701711605 2306 96-97 0.4974763983649808 2306 98-99 0.505080797478719 2306 100-101 0.34899394440677156 2307 1 0.13944896341139668 2307 2 0.19976273581580983 2307 3 0.12942084881171922 2307 4 0.11600046541786924 2307 5 0.12384757667973645 2307 6 0.12911503990456907 2307 7 0.15626333355326238 2307 8 0.11774487174159987 2307 9 0.12964102243813613 2307 10-11 0.20966819151017546 2307 12-13 0.13733194005057925 2307 14-15 0.39088008481763836 2307 16-17 0.3853796727002248 2307 18-19 0.48718019152777003 2307 20-21 0.38331742405885905 2307 22-23 0.398053811664667 2307 24-25 0.3566521082888343 2307 26-27 0.3924869190571769 2307 28-29 0.3798809700089123 2307 30-31 0.47445571211967064 2307 32-33 0.5507823803969245 2307 34-35 0.4900464361834551 2307 36-37 0.39514055535763504 2307 38-39 0.46330350000427245 2307 40-41 0.5048377639619659 2307 42-43 0.4993125895950712 2307 44-45 0.3960797342556006 2307 46-47 0.41798258186743453 2307 48-49 0.5112543752108252 2307 50-51 0.49552305430974997 2307 52-53 0.36062263909158077 2307 54-55 0.47680424499396423 2307 56-57 0.4877636360189612 2307 58-59 0.47292071214697984 2307 60-61 0.3024875596460106 2307 62-63 0.28328795337378665 2307 64-65 0.3568252836738637 2307 66-67 0.42660122963345515 2307 68-69 0.30382506501202755 2307 70-71 0.30284021762320634 2307 72-73 0.19427854888710527 2307 74-75 0.31772708009380324 2307 76-77 0.011752202404558432 2307 78-79 0.3573576392418616 2307 80-81 0.4317136676190998 2307 82-83 0.3924326052191347 2307 84-85 0.4523866131758254 2307 86-87 0.40383700965691816 2307 88-89 0.4437863838965157 2307 90-91 0.461377994123751 2307 92-93 0.490668709143101 2307 94-95 0.49372030324833105 2307 96-97 0.5261936848402229 2307 98-99 0.40614752016970357 2307 100-101 0.21918911581843048 2201 1 0.13911198510982814 2201 2 0.18488172458430085 2201 3 0.1215364628537472 2201 4 0.11418210084195124 2201 5 0.13502553417927743 2201 6 0.17112527613542028 2201 7 0.16495185355115183 2201 8 0.16866031572904916 2201 9 0.17040921761860517 2201 10-11 0.22902302224655102 2201 12-13 0.20239820695716304 2201 14-15 0.3191214206029258 2201 16-17 0.23617985198256264 2201 18-19 0.34738033521729506 2201 20-21 0.33639772705919313 2201 22-23 0.31066726377255094 2201 24-25 0.30351417293594096 2201 26-27 0.3241874318574105 2201 28-29 0.29654537092905286 2201 30-31 0.3868397058975219 2201 32-33 0.36843489397492135 2201 34-35 0.45444434906014664 2201 36-37 0.34753804474570416 2201 38-39 0.4073401787329516 2201 40-41 0.4831564234968866 2201 42-43 0.32044920339981076 2201 44-45 0.33315047827732513 2201 46-47 0.31403507468296255 2201 48-49 0.385287519374927 2201 50-51 0.37146354509516044 2201 52-53 0.3418142652219771 2201 54-55 0.5972546259689508 2201 56-57 0.4462254989734262 2201 58-59 0.3246100939524581 2201 60-61 0.3623792734401974 2201 62-63 0.43796367149185045 2201 64-65 0.3197706470501771 2201 66-67 0.3382585367072011 2201 68-69 0.40559580771549264 2201 70-71 0.3392565316573197 2201 72-73 0.23085181736241367 2201 74-75 0.2570567734023541 2201 76-77 0.005653430686347605 2201 78-79 0.21417708313702377 2201 80-81 0.29046496939346866 2201 82-83 0.3143047215417383 2201 84-85 0.19035920670584972 2201 86-87 0.23782782143682368 2201 88-89 0.22971844610485448 2201 90-91 0.2548366603158527 2201 92-93 0.14573063405543962 2201 94-95 0.12640550948894003 2201 96-97 0.17257388699425036 2201 98-99 0.12550577012357422 2201 100-101 0.32419555053856897 1101 1 0.07086292671822747 1101 2 0.12341761082662828 1101 3 0.08013971432780664 1101 4 0.07366323879307402 1101 5 0.05825645882396202 1101 6 0.12495298357907814 1101 7 0.11804585886635266 1101 8 0.10163385421096649 1101 9 0.10293652264212483 1101 10-11 0.14424532535121415 1101 12-13 0.12314055654643141 1101 14-15 0.16290289905231248 1101 16-17 0.28647471887575193 1101 18-19 0.2563129786258145 1101 20-21 0.3344343178427778 1101 22-23 0.24398065250435508 1101 24-25 0.182158292842594 1101 26-27 0.25633285536338235 1101 28-29 0.2857599856418318 1101 30-31 0.36489623408241556 1101 32-33 0.21733581682676117 1101 34-35 0.2979649096402497 1101 36-37 0.3384242798745518 1101 38-39 0.4304012142537772 1101 40-41 0.4020057459924615 1101 42-43 0.30100951709887624 1101 44-45 0.3978626999979298 1101 46-47 0.3106461124718578 1101 48-49 0.41539900753740255 1101 50-51 0.3473620781949265 1101 52-53 0.31070896708618534 1101 54-55 0.5189100530966329 1101 56-57 0.4751534514404767 1101 58-59 0.4903048213054362 1101 60-61 0.4850075219081802 1101 62-63 0.2663266949206431 1101 64-65 0.17099282991120646 1101 66-67 0.5064070080650964 1101 68-69 0.40812875410916405 1101 70-71 0.3290003623312501 1101 72-73 0.270387039744719 1101 74-75 0.2817514481937451 1101 76-77 0.06648374532863599 1101 78-79 0.3563076597952204 1101 80-81 0.35367018566278574 1101 82-83 0.35742466203914347 1101 84-85 0.3839369550107321 1101 86-87 0.42497838589785175 1101 88-89 0.3665877340825787 1101 90-91 0.3099603528464101 1101 92-93 0.2694407151514824 1101 94-95 0.2645797468239337 1101 96-97 0.30393077871809737 1101 98-99 0.3364541323261747 1101 100-101 0.4431893993930487 2304 1 0.12872170986771891 2304 2 0.17633128443763724 2304 3 0.07527003893309114 2304 4 0.08050278663334609 2304 5 0.06536149669603475 2304 6 0.06577223494488038 2304 7 0.12058383228714575 2304 8 0.08419521301120625 2304 9 0.09987734618584199 2304 10-11 0.129858309244554 2304 12-13 0.11185182500852164 2304 14-15 0.20006971502400717 2304 16-17 0.15707757996802485 2304 18-19 0.3021736790266729 2304 20-21 0.3189230229906599 2304 22-23 0.14814558699429625 2304 24-25 0.1879486781290396 2304 26-27 0.14549267479836203 2304 28-29 0.2730419797740282 2304 30-31 0.2932084408212532 2304 32-33 0.196634708525103 2304 34-35 0.14570116993494509 2304 36-37 0.20877305746783037 2304 38-39 0.2431234329873604 2304 40-41 0.3740843421701996 2304 42-43 0.30483921080763565 2304 44-45 0.34263083737868527 2304 46-47 0.20534978505746437 2304 48-49 0.2147803124288643 2304 50-51 0.13533617463457404 2304 52-53 0.17794002528653152 2304 54-55 0.40662895320480885 2304 56-57 0.3927306005385631 2304 58-59 0.23971700729789802 2304 60-61 0.2861808845143017 2304 62-63 0.18984088472693372 2304 64-65 0.2044832845907152 2304 66-67 0.3126637496439386 2304 68-69 0.3112598672357336 2304 70-71 0.20487610186804517 2304 72-73 0.07303287842693607 2304 74-75 0.18186225451241 2304 76-77 0.05981477880819952 2304 78-79 0.2801808175606837 2304 80-81 0.3726380971385552 2304 82-83 0.37244213748330424 2304 84-85 0.38707641438082163 2304 86-87 0.41079156040348863 2304 88-89 0.3250339735434622 2304 90-91 0.28987273318711715 2304 92-93 0.21999442879266695 2304 94-95 0.3185681781355427 2304 96-97 0.4775845495111781 2304 98-99 0.4408393679008853 2304 100-101 0.4952233391935792 2202 1 0.09902131411144666 2202 2 0.14289228992922887 2202 3 0.08147466024365713 2202 4 0.05287340704191479 2202 5 0.10909929557986686 2202 6 0.06980135729295256 2202 7 0.0277404040087319 2202 8 0.09421577748256027 2202 9 -0.014370058080814374 2202 10-11 0.12211319156571676 2202 12-13 0.10318871561331378 2202 14-15 0.1258522915355158 2202 16-17 0.1822113704403847 2202 18-19 0.21688054336079432 2202 20-21 0.19215925088313668 2202 22-23 0.16449036022984131 2202 24-25 0.15400673071395232 2202 26-27 0.12283394111130264 2202 28-29 0.12033976396027413 2202 30-31 0.18755455026537504 2202 32-33 0.2760228164116256 2202 34-35 0.2753451107004068 2202 36-37 0.29649739999572233 2202 38-39 0.2282792698299403 2202 40-41 0.3382510632921836 2202 42-43 0.25081428419733953 2202 44-45 0.1852563908143523 2202 46-47 0.24207370301240871 2202 48-49 0.2420532507058084 2202 50-51 0.2546579220183034 2202 52-53 0.3544426082541179 2202 54-55 0.34834179976829205 2202 56-57 0.41263603753626654 2202 58-59 0.34740338464791165 2202 60-61 0.30712432918288357 2202 62-63 0.27610589609470537 2202 64-65 0.3556245788276229 2202 66-67 0.4279383062258759 2202 68-69 0.39946248816136176 2202 70-71 0.2500818376379712 2202 72-73 0.21523753687423408 2202 74-75 0.26931649865292684 2202 76-77 0.0809819723524079 2202 78-79 0.3064490641100903 2202 80-81 0.3303871605963238 2202 82-83 0.2848829820442589 2202 84-85 0.34722651109704117 2202 86-87 0.3020924926428634 2202 88-89 0.3861906902411718 2202 90-91 0.21295093352627248 2202 92-93 0.13820653744192413 2202 94-95 0.3374857063567944 2202 96-97 0.4039660274178516 2202 98-99 0.32177514954540953 2202 100-101 0.2709180908791069 1102 1 0.10153472736357827 1102 2 0.14201635895703646 1102 3 0.09195950541735698 1102 4 0.06627570045003495 1102 5 0.05110561074312159 1102 6 0.039550044722403754 1102 7 0.03801489283866033 1102 8 0.05372505677245698 1102 9 0.058866474272818436 1102 10-11 0.13590626086094204 1102 12-13 0.05567067445772267 1102 14-15 0.09510895471008496 1102 16-17 0.09696526086658253 1102 18-19 0.12375910634325749 1102 20-21 0.19522801255074285 1102 22-23 0.04281042967555493 1102 24-25 0.0864150903977432 1102 26-27 0.05082063217470534 1102 28-29 0.06366045482622695 1102 30-31 0.19615321726303847 1102 32-33 0.2402663594718959 1102 34-35 0.17398974362635755 1102 36-37 0.17298934373754093 1102 38-39 0.1247131802693815 1102 40-41 0.2266655215207649 1102 42-43 0.11398313765621992 1102 44-45 0.1274677586833164 1102 46-47 0.07235964897856206 1102 48-49 0.16853508239070436 1102 50-51 0.2043404226005876 1102 52-53 0.11735250998665236 1102 54-55 0.20686655130402443 1102 56-57 0.2345838657124446 1102 58-59 0.15611847608614937 1102 60-61 0.20803990964210328 1102 62-63 0.0664194720982394 1102 64-65 0.08019067893155096 1102 66-67 0.23854034263118962 1102 68-69 0.19876796717093015 1102 70-71 0.03346707314027242 1102 72-73 0.07082969605239242 1102 74-75 0.062400810203961043 1102 76-77 -0.258408275407362 1102 78-79 0.14649948701133297 1102 80-81 0.3373367795490978 1102 82-83 0.3285080889556937 1102 84-85 0.30058350632409514 1102 86-87 0.2982564606842928 1102 88-89 0.253401746601277 1102 90-91 0.15614820298335985 1102 92-93 0.06811164385494095 1102 94-95 -0.06240237410003857 1102 96-97 0.16265186897262396 1102 98-99 0.24875210459899222 1102 100-101 0.19882331039381285 2305 1 0.04343183681010743 2305 2 0.11242843952586412 2305 3 0.055878291840635086 2305 4 0.034847852962343495 2305 5 0.013964378739871108 2305 6 0.005329864481005586 2305 7 0.03578672042245046 2305 8 0.02795226437592646 2305 9 0.00415770682689498 2305 10-11 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-1.409335806419925 1213 94-95 -1.3811199241517365 1213 96-97 -1.5787531835707398 1213 98-99 -1.5989173518831947 1213 100-101 -1.4815924221639065 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 6.0 8 49.0 9 130.0 10 214.0 11 139.0 12 142.0 13 209.0 14 474.0 15 1057.0 16 2008.0 17 3446.0 18 4741.0 19 6284.0 20 8230.0 21 10566.0 22 13349.0 23 16922.0 24 20901.0 25 26151.0 26 31934.0 27 40078.0 28 51910.0 29 66805.0 30 78113.0 31 93646.0 32 125773.0 33 177938.0 34 273401.0 35 476783.0 36 948674.0 37 1553938.0 38 458280.0 39 253.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.137232083020656 33.19731540152823 15.767267321496808 17.8981851939543 2 30.157723552624084 25.93866637700154 29.892728930423385 14.010881139950996 3 31.60374681048984 23.695364300324474 25.35447720080375 19.346411688381938 4 27.638528683023043 23.52081352974045 29.31565461495137 19.525003172285135 5 29.6550239686022 21.870281070146447 29.89197212091857 18.58272284033278 6 27.57942938344065 22.173962013505044 31.501305273804775 18.74530332924953 7 30.902312809846716 23.365847947176476 25.05290988802781 20.678929354949 8 27.248837184454956 26.965345247592452 25.25517835774118 20.530639210211408 9 26.285285130206848 24.149657744031 28.59237438742948 20.97268273833267 10-11 22.493101903954642 29.266958765456717 30.922401650378937 17.3175376802097 12-13 22.024592302267937 29.015564455239616 30.017046021140803 18.942797221351643 14-15 21.81459992378483 28.96233181021711 30.44969620776112 18.773372058236937 16-17 22.111703302182462 28.409482466949683 30.697506802381902 18.781307428485956 18-19 21.24983083081657 28.364174062624652 31.192972177901872 19.1930229286569 20-21 21.820844615458757 28.69257857718176 30.784334435196648 18.70224237216284 22-23 21.31241449012156 29.81018629453539 30.12925994034773 18.748139274995317 24-25 21.14788413869736 28.920851971622312 30.681001677446005 19.250262212234315 26-27 21.062053037210095 28.834241801527156 30.421838940253004 19.681866221009745 28-29 21.127272209242694 29.710126378283665 30.269787007094422 18.892814405379223 30-31 20.495102552139723 29.622993119266056 30.669282259672915 19.212622068921306 32-33 20.38317265228788 30.07279394481167 30.28205177289304 19.26198163000741 34-35 20.93415223089635 30.11436281981879 30.318545572397287 18.632939376887574 36-37 20.838014735858714 29.77838337829149 30.69657822725178 18.687023658598015 38-39 20.76692819703203 29.12426188948501 31.102951774275496 19.00585813920746 40-41 20.665779815043354 29.356391475539457 29.81346287065679 20.164365838760403 42-43 20.939323028979256 29.536415364710138 30.305596905911326 19.218664700399284 44-45 20.524349996566496 29.188877317740218 30.506908688055447 19.77986399763784 46-47 20.978472200349316 29.33030398374533 30.040822540978635 19.65040127492672 48-49 20.576561997415638 30.03458058656777 30.220316289381742 19.168541126634842 50-51 21.205696720622715 29.332459583602905 29.86175114181601 19.60009255395837 52-53 20.563550542061755 29.21095911453507 29.589074946945903 20.636415396457274 54-55 21.128339814692357 29.458077340803708 29.75428056914837 19.65930227535556 56-57 20.57465992320649 29.194169126070275 30.087748106602486 20.143422844120753 58-59 20.69289685104873 29.512191239370587 30.074473353798915 19.720438555781765 60-61 20.837557820317237 29.67897164584825 29.852266995073563 19.631203538760946 62-63 20.32594055839876 29.704450390185055 29.626427848682567 20.343181202733614 64-65 20.729115894329798 29.490944098050107 29.808537065480163 19.97140294213993 66-67 20.669205213744046 29.877598450153332 29.469485668126456 19.983710667976165 68-69 20.493449532270073 30.07057723892116 29.68556797494189 19.750405253866877 70-71 20.536442402162024 30.58399690503294 29.380956489550314 19.498604203254725 72-73 20.438332287946164 30.517768353335967 29.320137630397852 19.723761728320017 74-75 20.546806500619876 30.046311321862852 29.34268429751698 20.06419788000029 76-77 20.677047984494926 29.79269163803695 29.378582518096476 20.151677859371656 78-79 20.386457610298 29.586093946249136 29.827753782342082 20.199694661110787 80-81 20.44116419349201 29.83100560680252 29.539300490998727 20.18852970870674 82-83 20.055629355782454 29.88386064167427 29.541703373545335 20.518806628997943 84-85 20.132589945114084 29.94490260004074 29.567913945652812 20.354593509192362 86-87 20.16687222405674 29.765136858024675 29.94621959585762 20.121771322060965 88-89 20.346063845726235 30.134861757682064 29.56652245762835 19.952551938963357 90-91 20.631536528864533 29.84725881843492 29.167209348147704 20.353995304552846 92-93 20.25115772869714 30.15414892173031 29.158695433589983 20.43599791598256 94-95 19.7931602482469 30.447939731575136 29.16764104421202 20.59125897596595 96-97 19.969806838587388 30.61423788265306 29.013559421673698 20.40239585708585 98-99 20.156362256223943 30.29864407145743 28.85618655966407 20.68880711265455 100-101 20.154475875878216 30.126069014811314 28.838658715194914 20.880796394115556 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 58.0 1 46.0 2 35.0 3 67.5 4 89.5 5 76.0 6 67.0 7 60.0 8 61.0 9 70.5 10 84.0 11 89.0 12 92.0 13 99.0 14 122.0 15 159.5 16 207.5 17 279.5 18 386.0 19 581.5 20 863.5 21 1373.0 22 2238.0 23 3555.0 24 5452.5 25 8163.0 26 12388.5 27 17905.0 28 24223.0 29 31538.5 30 39311.0 31 47759.5 32 61416.0 33 83832.5 34 112178.0 35 148639.5 36 194984.5 37 250620.5 38 302889.5 39 318402.5 40 323046.0 41 310853.0 42 276553.5 43 263054.5 44 247118.5 45 232521.0 46 235325.0 47 217418.5 48 172353.5 49 130066.5 50 98891.5 51 78854.0 52 63344.5 53 48116.5 54 37027.0 55 28218.0 56 19426.0 57 12779.5 58 8334.5 59 5662.5 60 4061.5 61 3004.0 62 2040.5 63 1262.0 64 830.5 65 570.5 66 391.0 67 275.0 68 195.0 69 138.5 70 99.5 71 72.5 72 48.5 73 38.0 74 33.5 75 24.5 76 12.5 77 12.0 78 12.0 79 5.5 80 3.5 81 4.0 82 3.5 83 2.5 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15817318650635362 2 0.0 3 0.0018475055558721295 4 0.011441178984557524 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.5647757706991853E-5 22-23 1.6694327312097556E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.456799532736908E-4 38-39 0.0017028213858339505 40-41 0.0055202575645335915 42-43 0.002370594478317853 44-45 0.001079566499515642 46-47 0.0011240847056812355 48-49 0.003094015328508747 50-51 6.566435409425038E-4 52-53 0.01483569220468403 54-55 0.026755441905521683 56-57 0.02846939284289703 58-59 0.020867909140121942 60-61 0.030439323465724543 62-63 0.005620423528406177 64-65 0.010539685309704257 66-67 0.028068728987406692 68-69 0.027234012621801813 70-71 0.030750950908883697 72-73 0.030828857769673482 74-75 0.038764228018690526 76-77 0.018998144481167017 78-79 0.010817924098239215 80-81 0.015113930993218986 82-83 0.006933710610291185 84-85 0.011107292438315574 86-87 0.033455431933443504 88-89 0.015247485611715768 90-91 0.021346479856402076 92-93 0.0484914560658727 94-95 0.055603239500826254 96-97 0.03988831272437176 98-99 0.03477984856686991 100-101 0.034534998432959145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4492544.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.291058279890805 #Duplication Level Percentage of deduplicated Percentage of total 1 89.72934868705902 43.3312520686341 2 4.929040786818644 4.760571918004359 3 1.4042779584568887 2.0344220619902305 4 0.7219519413419181 1.3945529309849147 5 0.5368521124098682 1.2962578324033716 6 0.37510647304065514 1.086857313046234 7 0.26273168735232355 0.8881313858133575 8 0.20135813777618178 0.77790380531839 9 0.1868452058470889 0.8120657452391967 >10 1.3458012907470325 12.975070437885266 >50 0.1654854613649288 5.5987745260122095 >100 0.11116131340781076 10.300326557121075 >500 0.019649348055297475 6.700548132973879 >1k 0.010343155530235714 7.394885517297885 >5k 0.0 0.0 >10k+ 4.6440792002772416E-5 0.6483797672754277 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 28911 0.6435329292267365 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00102391874180865 0.0 0.0 2 0.0012465097726366175 0.0 0.0 3 0.0017584691435409425 0.0 0.0 4 0.0023594649267764545 0.0 2.2259103082796742E-5 5 0.002915942503846373 0.0 4.4518206165593484E-5 6 0.005364443842954015 0.0 4.4518206165593484E-5 7 0.006655471821756225 0.0 4.4518206165593484E-5 8 0.008480718274545558 0.0 4.4518206165593484E-5 9 0.009437859707105818 0.0 8.903641233118697E-5 10-11 0.01304383440651889 0.0 1.0016596387258533E-4 12-13 0.019287512821243375 0.0 1.1129551541398371E-4 14-15 0.027467733204171178 0.0 1.1129551541398371E-4 16-17 0.039031337255684084 0.0 1.3355461849678043E-4 18-19 0.05233115134765513 0.0 1.3355461849678043E-4 20-21 0.06703328893384239 0.0 1.3355461849678043E-4 22-23 0.08581997193572283 0.0 1.3355461849678043E-4 24-25 0.10657658556043079 0.0 1.3355461849678043E-4 26-27 0.1284795429939028 0.0 1.3355461849678043E-4 28-29 0.150193298051171 0.0 1.3355461849678043E-4 30-31 0.17514575260698614 0.0 1.3355461849678043E-4 32-33 0.20008707761125988 0.0 1.3355461849678043E-4 34-35 0.22498388440936806 0.0 1.3355461849678043E-4 36-37 0.2540431434839592 0.0 1.3355461849678043E-4 38-39 0.2854284788307026 0.0 1.446841700381788E-4 40-41 0.32116546883013275 0.0 1.5581372157957718E-4 42-43 0.360842320075218 0.0 1.5581372157957718E-4 44-45 0.4034462433756909 0.0 1.7807282466237394E-4 46-47 0.448509797566813 0.0 1.7807282466237394E-4 48-49 0.49545424596843124 0.0 1.7807282466237394E-4 50-51 0.5485755954755257 0.0 1.7807282466237394E-4 52-53 0.6026763455182631 0.0 1.7807282466237394E-4 54-55 0.6624976850532793 0.0 1.7807282466237394E-4 56-57 0.7227642066499516 0.0 1.7807282466237394E-4 58-59 0.7889405201151063 0.0 1.7807282466237394E-4 60-61 0.8569086913784261 0.0 1.7807282466237394E-4 62-63 0.9281489507949171 0.0 1.7807282466237394E-4 64-65 1.0020603025813437 0.0 1.7807282466237394E-4 66-67 1.0774852733774005 0.0 1.7807282466237394E-4 68-69 1.1564049233574563 0.0 2.0033192774517066E-4 70-71 1.240822571798963 0.0 2.1146147928656904E-4 72-73 1.3281672922958574 0.0 2.2259103082796741E-4 74-75 1.4189176555644196 0.0 2.2259103082796741E-4 76-77 1.515900567696165 0.0 2.2259103082796741E-4 78-79 1.6207409432161377 0.0 2.2259103082796741E-4 80-81 1.7297771596672176 0.0 2.4485013391076417E-4 82-83 1.8454020706308052 0.0 2.4485013391076417E-4 84-85 1.9662690003703915 0.0 2.4485013391076417E-4 86-87 2.0941030293748932 0.0 2.6710923699356086E-4 88-89 2.2282029958972025 0.0 2.6710923699356086E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGGCG 170 0.0 67.18027 1 GTCGCGG 25 0.004986388 57.103218 1 GCGACCG 25 0.00502369 56.995796 3 CGGCGAC 70 1.2751116E-9 54.38402 1 CCGAGCG 35 2.7640094E-4 54.38402 1 CGACGGG 145 0.0 52.508705 1 CGCGTGG 40 5.3420005E-4 47.586018 1 CGCCGAG 45 9.542721E-4 42.298683 1 TCGCCGG 80 2.2658787E-7 41.559437 2 GTCGGCG 85 3.5925223E-7 39.188488 1 GCGCAAC 65 1.3293762E-4 36.53577 2 CGCGGGG 145 1.6370905E-11 36.099735 1 CGGTTCG 55 0.0025576276 34.608013 1 CGAGGGG 550 0.0 34.608013 1 CGAGCGG 55 0.0025576276 34.608013 1 GTGTTTG 55665 0.0 32.271126 1 CGACCGC 60 0.003917315 31.724012 1 CGCGCCC 60 0.003917315 31.724012 1 TCGGCGG 60 0.0039535905 31.664333 2 GGTTCGC 60 0.0039535905 31.664333 2 >>END_MODULE