FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_34274-2.3410000003583d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_34274-2.3410000003583d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4492544
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT289110.6435329292267365TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGCG1700.067.180271
GTCGCGG250.00498638857.1032181
GCGACCG250.0050236956.9957963
CGGCGAC701.2751116E-954.384021
CCGAGCG352.7640094E-454.384021
CGACGGG1450.052.5087051
CGCGTGG405.3420005E-447.5860181
CGCCGAG459.542721E-442.2986831
TCGCCGG802.2658787E-741.5594372
GTCGGCG853.5925223E-739.1884881
GCGCAAC651.3293762E-436.535772
CGCGGGG1451.6370905E-1136.0997351
CGGTTCG550.002557627634.6080131
CGAGGGG5500.034.6080131
CGAGCGG550.002557627634.6080131
GTGTTTG556650.032.2711261
CGACCGC600.00391731531.7240121
CGCGCCC600.00391731531.7240121
TCGGCGG600.003953590531.6643332
GGTTCGC600.003953590531.6643332
Produced by FastQC (version 0.11.2)