FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_34271-1.341000000358dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_34271-1.341000000358dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5067364
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3236416.386772294234241TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG438000.081.020733
GATCGGA441950.080.472641
ATCGGAA441450.080.322982
CGGAAGA439400.080.274544
GAAGAGC454800.077.437416
GGAAGAG496150.071.270785
GAGCACA519500.067.646879
AGAGCAC522400.067.4167948
AAGAGCA537750.065.43947
GCCGTCT382600.045.4061250-51
ATGCCGT381900.045.33627348-49
TGCCGTC382200.045.14912450-51
TCGTATG389650.045.05978844-45
TATGCCG385050.045.0579148-49
GTATGCC388650.044.99076546-47
CGTATGC387200.044.9384546-47
CGTATCT383300.044.85976438-39
TATCTCG384800.044.8389540-41
TCTCGTA387950.044.7859442-43
TCGTATC383000.044.591138-39
Produced by FastQC (version 0.11.2)