FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_34211-2.34100000035863.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_34211-2.34100000035863.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3372912
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT742052.200027750501644TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG225800.037.9224433
CGGAAGA224200.037.874214
GAGCACA224750.037.4834759
AGAGCAC226700.037.370538
ATCGGAA231550.036.9397132
GCCGTCT115300.036.7315250-51
GATCGGA233950.036.6996351
CGTCTTC115750.036.56928352-53
GAAGAGC235950.036.0061156
ATGCCGT118650.035.63067648-49
TGCCGTC116850.035.55314350-51
CCGTCTT116650.035.53370352-53
CTGCTTG121500.035.11286558-59
TATGCCG120200.034.5586848-49
AAGAGCA245850.034.440317
GCTTGAA119850.034.2477760-61
CGTATGC121350.034.2266146-47
TGCTTGA120050.034.210560-61
TCGTATG124600.033.998944-45
GTATGCC124900.033.93826746-47
Produced by FastQC (version 0.11.2)