##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_34211-1.3410000003589a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3662890 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67119050804146 33.0 33.0 33.0 33.0 33.0 2 32.64532732350685 33.0 33.0 33.0 33.0 33.0 3 32.70816459134727 33.0 33.0 33.0 33.0 33.0 4 36.65055680077753 37.0 37.0 37.0 37.0 37.0 5 36.65040282399963 37.0 37.0 37.0 37.0 37.0 6 36.5191564584249 37.0 37.0 37.0 37.0 37.0 7 36.45851035657636 37.0 37.0 37.0 37.0 37.0 8 36.392697023388635 37.0 37.0 37.0 37.0 37.0 9 36.38062813789112 37.0 37.0 37.0 37.0 37.0 10-11 36.24311390732454 37.0 37.0 37.0 35.0 37.0 12-13 36.277329513034786 37.0 37.0 37.0 35.0 37.0 14-15 38.0850002320572 40.0 37.0 40.0 37.0 40.0 16-17 38.02997482861893 40.0 37.0 40.0 35.0 40.0 18-19 37.93751982724024 40.0 37.0 40.0 33.0 40.0 20-21 37.903002410664804 40.0 37.0 40.0 33.0 40.0 22-23 37.821378883886766 40.0 37.0 40.0 33.0 40.0 24-25 37.784489023694405 40.0 37.0 40.0 33.0 40.0 26-27 37.69868464518454 40.0 37.0 40.0 33.0 40.0 28-29 37.53887313023323 40.0 37.0 40.0 33.0 40.0 30-31 37.39775013718676 40.0 37.0 40.0 33.0 40.0 32-33 37.267646858082 40.0 37.0 40.0 33.0 40.0 34-35 37.16154470923233 40.0 37.0 40.0 33.0 40.0 36-37 37.09872982808656 40.0 37.0 40.0 33.0 40.0 38-39 36.94445669949138 40.0 37.0 40.0 33.0 40.0 40-41 36.74734608464901 37.0 37.0 40.0 33.0 40.0 42-43 36.69567964093926 37.0 37.0 40.0 33.0 40.0 44-45 36.54029755193304 37.0 37.0 40.0 33.0 40.0 46-47 36.43246125327269 37.0 37.0 40.0 33.0 40.0 48-49 36.26170687626437 37.0 37.0 40.0 33.0 40.0 50-51 36.102401246010665 37.0 37.0 40.0 33.0 40.0 52-53 35.89966065046998 37.0 37.0 40.0 27.0 40.0 54-55 35.529104341107704 37.0 35.0 40.0 27.0 40.0 56-57 35.36574194147244 37.0 33.0 40.0 27.0 40.0 58-59 35.207962019061455 37.0 33.0 40.0 27.0 40.0 60-61 34.940023314923465 37.0 33.0 40.0 27.0 40.0 62-63 34.705969330228314 37.0 33.0 40.0 27.0 40.0 64-65 34.48802366437431 37.0 33.0 38.5 27.0 40.0 66-67 34.04282465484904 37.0 33.0 37.0 27.0 40.0 68-69 33.782793231573976 37.0 33.0 37.0 27.0 40.0 70-71 33.512049501896044 37.0 33.0 37.0 27.0 40.0 72-73 33.17666214382632 37.0 33.0 37.0 22.0 40.0 74-75 32.79839006904385 37.0 33.0 37.0 22.0 38.5 76-77 30.20766362080215 33.0 27.0 35.0 22.0 37.0 78-79 32.5335513760992 35.0 33.0 37.0 22.0 37.0 80-81 32.840938166311304 37.0 33.0 37.0 22.0 37.0 82-83 32.672144536144955 37.0 33.0 37.0 22.0 37.0 84-85 32.5136422333185 37.0 33.0 37.0 22.0 37.0 86-87 32.34322433925125 35.0 33.0 37.0 22.0 37.0 88-89 32.11566795617668 33.0 33.0 37.0 22.0 37.0 90-91 31.960715992017235 33.0 33.0 37.0 22.0 37.0 92-93 31.76421145598148 33.0 33.0 37.0 22.0 37.0 94-95 31.601375553183416 33.0 33.0 37.0 22.0 37.0 96-97 31.406239881623527 33.0 33.0 37.0 15.0 37.0 98-99 31.253924496777135 33.0 33.0 37.0 15.0 37.0 100-101 29.450218543281398 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.0934143609669178 2306 2 0.16905736150880557 2306 3 0.1389378743928802 2306 4 0.11459805646985899 2306 5 0.13773366520046437 2306 6 0.1950591180961183 2306 7 0.2190102452813676 2306 8 0.19205492649522427 2306 9 0.16839884160691554 2306 10-11 0.22735910291959271 2306 12-13 0.25867111159457323 2306 14-15 0.4268061677786861 2306 16-17 0.3699983760505816 2306 18-19 0.5056257767129253 2306 20-21 0.4248191141980442 2306 22-23 0.3915456676423261 2306 24-25 0.42643735036246966 2306 26-27 0.38738275640691455 2306 28-29 0.3550633837606796 2306 30-31 0.44462000257320256 2306 32-33 0.49147187373762335 2306 34-35 0.5168699170714248 2306 36-37 0.35294339913074424 2306 38-39 0.39713014246424194 2306 40-41 0.48068824720214565 2306 42-43 0.3918872983149342 2306 44-45 0.3888973617477234 2306 46-47 0.4257763600540443 2306 48-49 0.31971580589803494 2306 50-51 0.25011997472548586 2306 52-53 0.16154910948503698 2306 54-55 0.41372861107080183 2306 56-57 0.5049517435814224 2306 58-59 0.20176711510194423 2306 60-61 0.18850988730318363 2306 62-63 0.21377446211897677 2306 64-65 0.14308866023422695 2306 66-67 0.1824134918517828 2306 68-69 0.24998555566433112 2306 70-71 0.0980558769289388 2306 72-73 0.04409007810488674 2306 74-75 0.2743101397166967 2306 76-77 0.0922968772346131 2306 78-79 0.338005892166926 2306 80-81 0.34932616749681245 2306 82-83 0.26255281480147374 2306 84-85 0.35275434791640947 2306 86-87 0.47592376652714563 2306 88-89 0.3796168104099493 2306 90-91 0.26559276297442125 2306 92-93 0.2557479087942589 2306 94-95 0.1693561150735441 2306 96-97 0.2539352021820598 2306 98-99 0.28439369647333734 2306 100-101 0.4585505103816807 2307 1 0.13650213953977186 2307 2 0.1918166066971807 2307 3 0.1477834153830173 2307 4 0.15346215054098877 2307 5 0.1404926521948653 2307 6 0.10540863896187602 2307 7 0.13391854953714244 2307 8 0.1350805540909974 2307 9 0.13273303312782758 2307 10-11 0.2263648717105795 2307 12-13 0.22331070969720912 2307 14-15 0.33275246712139506 2307 16-17 0.3566787024077698 2307 18-19 0.47728133489140845 2307 20-21 0.44210805906995176 2307 22-23 0.40092852872658824 2307 24-25 0.43274567453605783 2307 26-27 0.3788748962447599 2307 28-29 0.39318867422889525 2307 30-31 0.40155573809405354 2307 32-33 0.39668038381088166 2307 34-35 0.3977413401628169 2307 36-37 0.3878762133712499 2307 38-39 0.415914860332002 2307 40-41 0.5406255798371191 2307 42-43 0.42456534149972214 2307 44-45 0.43365464097342965 2307 46-47 0.3978933477418849 2307 48-49 0.5302334294807309 2307 50-51 0.4768462780517666 2307 52-53 0.34225621055691136 2307 54-55 0.5630320406244209 2307 56-57 0.47598496207991303 2307 58-59 0.5642145541866057 2307 60-61 0.4887505609681355 2307 62-63 0.3356431225348899 2307 64-65 0.35005851424398315 2307 66-67 0.5763212207038535 2307 68-69 0.24320694961668465 2307 70-71 0.2705566515108515 2307 72-73 0.16037252755419473 2307 74-75 0.24532777436485986 2307 76-77 0.06040346708242694 2307 78-79 0.18274666542097862 2307 80-81 0.38067488058429433 2307 82-83 0.38686131602236173 2307 84-85 0.4025919623516714 2307 86-87 0.4052230649772426 2307 88-89 0.3322066803539556 2307 90-91 0.46742372707070245 2307 92-93 0.375215826349244 2307 94-95 0.372829100607305 2307 96-97 0.5933381839535627 2307 98-99 0.4017634191730117 2307 100-101 0.31904895384089116 2201 1 0.15279096313017249 2201 2 0.19265657851305207 2201 3 0.12385646549801521 2201 4 0.1637052671891297 2201 5 0.1532996097219126 2201 6 0.18810357031001956 2201 7 0.1698178737549938 2201 8 0.21183624459795425 2201 9 0.21174655611886806 2201 10-11 0.2774037734768271 2201 12-13 0.26135307242820005 2201 14-15 0.3563969225970922 2201 16-17 0.3017382752533635 2201 18-19 0.4676629717750558 2201 20-21 0.3577073023768236 2201 22-23 0.32123212040622917 2201 24-25 0.3032976735105919 2201 26-27 0.21265281808199177 2201 28-29 0.292416468412938 2201 30-31 0.4403965965831347 2201 32-33 0.4549384057131718 2201 34-35 0.48223011101004687 2201 36-37 0.46355303650117463 2201 38-39 0.32838723344017495 2201 40-41 0.5798954052702712 2201 42-43 0.4539421474023868 2201 44-45 0.4902752566969326 2201 46-47 0.42926182087593645 2201 48-49 0.41777795704965826 2201 50-51 0.35866882420816637 2201 52-53 0.4327922396162762 2201 54-55 0.5429814692264117 2201 56-57 0.513355543308073 2201 58-59 0.4036304439256071 2201 60-61 0.4287765079979877 2201 62-63 0.37079889860663684 2201 64-65 0.4369683274955847 2201 66-67 0.42132013073264574 2201 68-69 0.46658066767583506 2201 70-71 0.37853524072070854 2201 72-73 0.40248199332134504 2201 74-75 0.45815081611116426 2201 76-77 0.2557197750927749 2201 78-79 0.4820743123976783 2201 80-81 0.4740414056499631 2201 82-83 0.5974190138725461 2201 84-85 0.6201382942924027 2201 86-87 0.6477432090330666 2201 88-89 0.594574261099595 2201 90-91 0.6805376727419699 2201 92-93 0.5835557797948105 2201 94-95 0.5621309720102943 2201 96-97 0.5849378126285458 2201 98-99 0.5288892508373699 2201 100-101 0.5009050564141191 1101 1 0.07985414376899769 1101 2 0.13704375472872954 1101 3 0.12305195539796188 1101 4 0.12743052518806763 1101 5 0.12258998453194181 1101 6 0.14001430702418105 1101 7 0.1410599171270448 1101 8 0.16492325839801936 1101 9 0.17637737449203428 1101 10-11 0.24359798806752764 1101 12-13 0.12687569911259544 1101 14-15 0.30526915973339896 1101 16-17 0.3286301893718715 1101 18-19 0.3393516112304198 1101 20-21 0.36506371909085544 1101 22-23 0.43217674442353626 1101 24-25 0.35397647620467154 1101 26-27 0.36356340213959726 1101 28-29 0.36696036192757475 1101 30-31 0.3750134654056083 1101 32-33 0.3272231120496869 1101 34-35 0.42449869110665617 1101 36-37 0.40317701127694505 1101 38-39 0.45703605288898785 1101 40-41 0.5871080307596444 1101 42-43 0.5062321325706307 1101 44-45 0.4950091953612912 1101 46-47 0.3768709295851238 1101 48-49 0.33497223059836756 1101 50-51 0.34201498943674835 1101 52-53 0.196128965797854 1101 54-55 0.5866525579357216 1101 56-57 0.6176348854751197 1101 58-59 0.5679139001794198 1101 60-61 0.3739706448909814 1101 62-63 0.4879324554998874 1101 64-65 0.308053692489608 1101 66-67 0.5798992918020289 1101 68-69 0.6077866774715162 1101 70-71 0.37266264853899855 1101 72-73 0.2570185379872214 1101 74-75 0.25586676278141596 1101 76-77 0.05644394950410714 1101 78-79 0.45993990573072097 1101 80-81 0.41376145746958315 1101 82-83 0.4160075361470703 1101 84-85 0.4593365317219096 1101 86-87 0.5691535566099688 1101 88-89 0.49011328791193165 1101 90-91 0.4590813840489787 1101 92-93 0.40055935518703834 1101 94-95 0.37187182923087647 1101 96-97 0.34398422670604134 1101 98-99 0.2860273859855518 1101 100-101 0.2662963754027352 2304 1 0.1432462495018143 2304 2 0.15465086699291675 2304 3 0.12641114913965623 2304 4 0.08624507702315753 2304 5 0.07022958657297096 2304 6 0.06485654005589225 2304 7 0.14848816854605928 2304 8 0.14485862594072785 2304 9 0.05808407337477206 2304 10-11 0.11952099897852975 2304 12-13 0.13185186990889264 2304 14-15 0.1644437981768121 2304 16-17 0.22203079896639366 2304 18-19 0.27212668059141976 2304 20-21 0.2856614035434575 2304 22-23 0.19077553958459248 2304 24-25 0.1444401451845323 2304 26-27 0.10176957170033774 2304 28-29 0.12249098036269146 2304 30-31 0.2008203544401823 2304 32-33 0.2369512397314324 2304 34-35 0.298671261218189 2304 36-37 0.2153296072860158 2304 38-39 0.2798283190632347 2304 40-41 0.3704023459682517 2304 42-43 0.27175511229475546 2304 44-45 0.2606585155833656 2304 46-47 0.29335707590594495 2304 48-49 0.19066340701944284 2304 50-51 0.24886208751660632 2304 52-53 0.10191625424713635 2304 54-55 0.41641634588675913 2304 56-57 0.36559180874013464 2304 58-59 0.26040669728162413 2304 60-61 0.4746057406364841 2304 62-63 0.2965637715210505 2304 64-65 0.1384489886599738 2304 66-67 0.3027981865072249 2304 68-69 0.3103189788061016 2304 70-71 0.12677062504730685 2304 72-73 0.09207075397753783 2304 74-75 0.29091378527714795 2304 76-77 0.16806840208257867 2304 78-79 0.24509047433419795 2304 80-81 0.3988201879930031 2304 82-83 0.38888085192008504 2304 84-85 0.3327776091298489 2304 86-87 0.42174165239509875 2304 88-89 0.46046147740273824 2304 90-91 0.3482430141483448 2304 92-93 0.3394803620048883 2304 94-95 0.36209873443392837 2304 96-97 0.3228733187873516 2304 98-99 0.43260353906439164 2304 100-101 0.3548632014315487 2202 1 0.10704601687041304 2202 2 0.17509465612597097 2202 3 0.12788024066557568 2202 4 0.1459139594045027 2202 5 0.12497485705357292 2202 6 0.1480905359289224 2202 7 0.11442668153184599 2202 8 0.1617758963989644 2202 9 0.09431925663723462 2202 10-11 0.18777340684400912 2202 12-13 0.15253868242997015 2202 14-15 0.23889493346651136 2202 16-17 0.253887949679104 2202 18-19 0.32395101033151263 2202 20-21 0.2789674256769885 2202 22-23 0.3071494908326571 2202 24-25 0.2734581862342864 2202 26-27 0.21954760630053016 2202 28-29 0.2776927526858941 2202 30-31 0.3139822261909373 2202 32-33 0.2825232510796667 2202 34-35 0.2641963082384393 2202 36-37 0.2949622901337321 2202 38-39 0.3231823633638484 2202 40-41 0.42687103206684895 2202 42-43 0.3976908571905682 2202 44-45 0.4182045432344381 2202 46-47 0.37627593358884326 2202 48-49 0.181890216110709 2202 50-51 0.10020866543041507 2202 52-53 0.25895130367199215 2202 54-55 0.40536216029238403 2202 56-57 0.4243603628651442 2202 58-59 0.4087872714412768 2202 60-61 0.3191476636854489 2202 62-63 0.34310589588564966 2202 64-65 0.3179106930205009 2202 66-67 0.3176580298165561 2202 68-69 0.39113089594607686 2202 70-71 0.28060466267469053 2202 72-73 0.21699828966912804 2202 74-75 0.2398478842312315 2202 76-77 -0.020401827435883035 2202 78-79 0.20610863747815245 2202 80-81 0.38017635635997493 2202 82-83 0.4600050366383712 2202 84-85 0.36518686331134376 2202 86-87 0.3614483187386668 2202 88-89 0.33309636819832633 2202 90-91 0.29840539478029626 2202 92-93 0.33203948915248915 2202 94-95 0.3869500311489418 2202 96-97 0.3823504387062293 2202 98-99 0.45630018343100076 2202 100-101 0.3971125727861953 1102 1 0.06510407239787952 1102 2 0.11572237595280654 1102 3 0.09482021886806535 1102 4 0.07595504364469718 1102 5 0.06379123163190314 1102 6 0.031620042511825375 1102 7 -0.021254162157894996 1102 8 0.07127269980566808 1102 9 0.12579653908782262 1102 10-11 0.16884897600355941 1102 12-13 0.07902816439776217 1102 14-15 0.12820657041441308 1102 16-17 0.10598916850724294 1102 18-19 0.21096934979701842 1102 20-21 0.1626894538650916 1102 22-23 0.0703241483343433 1102 24-25 0.07455803342701728 1102 26-27 -0.0017174097364502927 1102 28-29 0.10774435971191565 1102 30-31 0.1304718097133346 1102 32-33 0.18970606795380718 1102 34-35 0.09462750261568686 1102 36-37 0.19281488556697468 1102 38-39 0.14666182081160883 1102 40-41 0.29421548930285013 1102 42-43 0.16363568667821937 1102 44-45 0.08122550756240798 1102 46-47 0.06986996624015518 1102 48-49 0.24743977130152928 1102 50-51 0.12222851308986549 1102 52-53 0.21390664989541364 1102 54-55 0.06435648447024533 1102 56-57 0.1951441613185736 1102 58-59 0.09475784826280176 1102 60-61 0.08381236494636823 1102 62-63 -0.004440112672597252 1102 64-65 -0.013507579721462548 1102 66-67 0.021794132868116378 1102 68-69 0.08446558622181755 1102 70-71 -0.22345103937878008 1102 72-73 -0.2167101042728632 1102 74-75 -0.05374205382477726 1102 76-77 -0.2716533799249241 1102 78-79 0.08528188358688027 1102 80-81 0.19094112304799893 1102 82-83 0.09220412385692356 1102 84-85 0.05424642876133845 1102 86-87 0.24062523809912761 1102 88-89 0.06496438739949184 1102 90-91 0.03803910027884427 1102 92-93 0.07177485729442523 1102 94-95 -0.04902735199195973 1102 96-97 0.006201683247127221 1102 98-99 0.07958493692988 1102 100-101 0.19113114690766153 2305 1 0.07528643953423853 2305 2 0.07196163877588191 2305 3 0.08096651278802369 2305 4 0.05901675542137497 2305 5 0.024755263193505073 2305 6 -0.03514532092196987 2305 7 -0.019362508362483766 2305 8 0.06389899941240174 2305 9 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-1.406588170100079 1213 86-87 -1.5196051640022716 1213 88-89 -1.5633296807434505 1213 90-91 -1.438256068134507 1213 92-93 -1.582919020188431 1213 94-95 -1.5144048250743296 1213 96-97 -1.6100427705165465 1213 98-99 -1.5953604796038015 1213 100-101 -1.506481126017519 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 6.0 8 37.0 9 140.0 10 202.0 11 168.0 12 170.0 13 395.0 14 825.0 15 1935.0 16 3470.0 17 5326.0 18 7627.0 19 10113.0 20 12777.0 21 15851.0 22 19184.0 23 23664.0 24 28507.0 25 34248.0 26 41395.0 27 50668.0 28 62443.0 29 79762.0 30 93514.0 31 108804.0 32 139540.0 33 189213.0 34 272524.0 35 420235.0 36 676221.0 37 979267.0 38 383453.0 39 1205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.35282642383546 24.641376702194247 14.965828277008486 16.039968596961803 2 36.92417735722339 21.961019850445958 28.45100453467071 12.663798257659936 3 43.073977063686684 18.960598312019258 22.297246015248284 15.668178609045771 4 35.6640236776864 20.026784836669833 28.15315684282953 16.156034642814237 5 38.16243458034503 17.560205193167143 27.978536073974375 16.298824152513454 6 34.49827868158749 18.337433010546317 30.482788180917254 16.68150012694894 7 40.14070856618681 19.137921149693273 21.345058137154 19.376312146965923 8 34.43851712718646 26.633232229196075 20.178383735247305 18.749866908370166 9 34.39297931414812 21.601385791001096 23.34877651253518 20.656858382315605 10-11 28.859411557540632 28.713242821924766 27.74664267832231 14.680702942212298 12-13 28.441886051724186 28.07971301349481 26.83538135188335 16.643019582897658 14-15 27.22815863976259 28.650860386197785 27.148931581346968 16.972049392692657 16-17 27.103926134827965 28.07575439065874 27.314825179025306 17.50549429548799 18-19 25.763372637452942 28.382588611724625 27.929326296994994 17.924712453827443 20-21 25.720029424865103 28.34788719339942 28.178403464915736 17.753679916819745 22-23 25.085311862822866 29.761148624806488 27.202127176000225 17.95141233637042 24-25 24.461490790059216 28.683048084982076 28.127216487527605 18.7282446374311 26-27 24.468670912858425 28.355642675592225 27.56584281810265 19.609843593446705 28-29 24.74685289484533 29.311390732454427 27.266161418988833 18.675594953711414 30-31 23.895735334667435 28.53677014597763 28.24683514929468 19.320659370060252 32-33 23.90313386424381 29.117882327888633 27.46563505865587 19.513348749211687 34-35 23.53378070321522 29.2240826232838 28.297682977102777 18.9444536963982 36-37 23.602039770250787 28.27858586594887 28.338170395921114 19.78120396787923 38-39 23.95701078306897 29.22551183505739 27.563680006049974 19.253797375823662 40-41 23.165121552570398 29.632608630660464 27.781884521815957 19.42038529495318 42-43 23.265355487902596 28.948176687249223 28.124137445257208 19.662330379590976 44-45 22.941345496911897 28.42012942116755 28.337270388679848 20.301254693240708 46-47 23.650103819529427 28.159301788922903 27.66534169531811 20.525252696229558 48-49 22.8697800222181 29.029747631511484 28.141474001024907 19.95899834524551 50-51 23.29615064510374 28.26052207011433 28.3432170021217 20.100110282660232 52-53 22.849699593596718 28.26939753798904 27.60443231872677 21.27647054968747 54-55 22.99952483423815 28.46350781565753 28.46658000808328 20.070387342021036 56-57 22.57873443232696 28.23723624903908 28.421406207543487 20.762623111090477 58-59 22.411920256374966 28.65087479001326 28.215800833174953 20.721404120436816 60-61 22.85256385310335 28.39007473077382 28.431666928154055 20.325694487968782 62-63 22.349032290563056 28.498560691163107 28.17437321775725 20.978033800516588 64-65 22.762845141308397 28.53651880469193 28.287932011532547 20.412704042467126 66-67 22.358525191585162 29.281711980224028 27.62392887904314 20.735833949147665 68-69 22.209648205938358 29.60342010815505 27.48473629270271 20.702195393203887 70-71 22.06924190836156 29.407472292790764 27.75475509851566 20.768530700332015 72-73 22.110064897070956 29.477938177439217 27.539997239003565 20.871999686486262 74-75 21.753893058718603 29.5300280314703 27.88688160783499 20.829197301976105 76-77 21.96051863948543 29.21443733251851 27.80065363091193 21.024390397084126 78-79 21.834107873745612 29.46748452455014 27.72884710134036 20.96956050036389 80-81 21.765446968110393 29.32375715830779 27.84745217077655 21.063343702805273 82-83 21.914841567484803 29.39189631936815 27.498053312481296 21.195208800665753 84-85 21.655841394437587 29.43937526732122 27.767563883031087 21.137219455210104 86-87 21.926282253920625 29.003843508428012 27.67211365107083 21.39776058658053 88-89 21.838898896944634 29.372059161830833 27.458822292654055 21.330219648570477 90-91 21.75857703173173 29.07785073092794 27.702052228897067 21.461520008443262 92-93 21.85206202357125 29.044537360071725 27.465622697453107 21.637777918903915 94-95 21.53888179788663 29.13119401323638 27.731915041825534 21.598009147051453 96-97 21.68465562916349 28.84690151802654 27.708924443626294 21.759518409183677 98-99 21.558338026214955 29.17443427334802 27.530701277647264 21.736526422789762 100-101 21.610184766928867 28.898064936888 27.64699504531643 21.8447552508667 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6.0 1 5.5 2 37.0 3 104.5 4 92.5 5 37.5 6 28.5 7 25.5 8 34.0 9 37.5 10 31.0 11 32.5 12 38.5 13 45.5 14 58.5 15 86.0 16 115.5 17 155.0 18 233.0 19 370.5 20 607.5 21 1018.0 22 1591.5 23 2501.5 24 3904.0 25 5788.0 26 8422.5 27 11894.5 28 16373.5 29 21963.0 30 28462.0 31 36495.0 32 46542.0 33 57938.0 34 70737.5 35 85136.0 36 101229.5 37 118770.5 38 135117.0 39 148740.5 40 161105.0 41 171394.0 42 178256.0 43 182103.0 44 183373.0 45 183275.0 46 182717.0 47 180846.5 48 175971.0 49 167869.0 50 157241.5 51 144055.5 52 127758.5 53 109233.5 54 90749.5 55 73987.5 56 59923.0 57 48264.5 58 38875.5 59 31580.5 60 25066.0 61 19512.0 62 15245.5 63 11789.0 64 9059.0 65 6927.0 66 5161.0 67 3848.5 68 2978.0 69 2267.5 70 1729.5 71 1379.5 72 1059.5 73 803.0 74 632.0 75 480.5 76 366.5 77 284.0 78 203.0 79 158.0 80 125.5 81 100.5 82 83.5 83 67.5 84 48.5 85 30.0 86 25.0 87 20.5 88 18.0 89 12.5 90 10.5 91 6.5 92 3.0 93 3.0 94 1.5 95 0.5 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16159371425295327 2 0.0 3 0.00141964405155492 4 0.01010131344375616 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.095127071793038E-5 22-23 1.5015465929907806E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.234724493501034E-4 38-39 0.0017609046408710062 40-41 0.0058833325598093315 42-43 0.0021567669244776664 44-45 0.001160286003674694 46-47 0.001187586850819981 48-49 0.003030394033126848 50-51 8.053749907859641E-4 52-53 0.014141838821258624 54-55 0.027573855616739786 56-57 0.0288023937382777 58-59 0.02058483874754634 60-61 0.031273120404926164 62-63 0.005310014769758306 64-65 0.010551777421653395 66-67 0.029266508139747578 68-69 0.02777861197032944 70-71 0.03173723480639604 72-73 0.03190103988926776 74-75 0.04011859487999912 76-77 0.01997056968677738 78-79 0.010797485045960977 80-81 0.014660554917019075 82-83 0.007043618563484025 84-85 0.010797485045960977 86-87 0.034440018673779445 88-89 0.015356726519223892 90-91 0.021799726445511602 92-93 0.050847827808096886 94-95 0.05862856924450366 96-97 0.04162014147298991 98-99 0.035927914843197586 100-101 0.03560030467745414 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3662890.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.2552860235915 #Duplication Level Percentage of deduplicated Percentage of total 1 97.03201186429888 90.48748019849715 2 2.217973216758561 4.1367545344296985 3 0.3146708330004934 0.8803415560422843 4 0.13976167199831918 0.5213405878935454 5 0.08528211815549633 0.39765041606442675 6 0.0466901305159452 0.26124608854459724 7 0.026709508827570345 0.17435620196853 8 0.025067779373001958 0.18701623483244675 9 0.010409949702166459 0.08737045532700606 >10 0.09711009500555576 1.58152242001163 >50 0.0038532369136449213 0.22536748035185059 >100 3.109040236345704E-4 0.06547232630181263 >500 1.1939389464492726E-4 0.06886631278380555 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 2.9297532113607955E-5 0.9252151869511804 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 33864 0.9245158877279962 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.463262615038945E-4 0.0 0.0 2 0.0012558389686831983 0.0 0.0 3 0.0018564576058795104 0.0 0.0 4 0.0024843770902211095 0.0 0.0 5 0.003248800810289143 0.0 2.730084714528692E-5 6 0.0054874702762026705 0.0 8.190254143586075E-5 7 0.007562334659244476 0.0 8.190254143586075E-5 8 0.009992110055175012 0.0 8.190254143586075E-5 9 0.011493656648165793 0.0 8.190254143586075E-5 10-11 0.016039247697856064 0.0 8.190254143586075E-5 12-13 0.02114450611402472 0.0 1.2285381215379113E-4 14-15 0.024966624714364886 0.0 1.365042357264346E-4 16-17 0.029484914916909872 0.0 1.638050828717215E-4 18-19 0.034958734769539895 0.0 1.638050828717215E-4 20-21 0.04110142537722945 0.0 1.638050828717215E-4 22-23 0.0472714168320643 0.0 1.638050828717215E-4 24-25 0.05316839981544627 0.0 1.638050828717215E-4 26-27 0.058382861620196076 0.0 1.638050828717215E-4 28-29 0.06391128316711668 0.0 1.638050828717215E-4 30-31 0.09321874257758218 0.0 1.638050828717215E-4 32-33 0.10119058994400595 0.0 1.638050828717215E-4 34-35 0.10730597970455023 0.0 2.047563535896519E-4 36-37 0.1142676957265984 0.0 2.1840677716229536E-4 38-39 0.12320872316667986 0.0 2.4570762430758226E-4 40-41 0.13341923999901717 0.0 3.139597421707996E-4 42-43 0.14525415723649904 0.0 3.4126058931608646E-4 44-45 0.15850871852553583 1.365042357264346E-5 3.549110128887299E-4 46-47 0.171940735321017 2.730084714528692E-5 3.8221186003401685E-4 48-49 0.1880482351367363 2.730084714528692E-5 3.8221186003401685E-4 50-51 0.20568458239259163 2.730084714528692E-5 3.8221186003401685E-4 52-53 0.22480882581786513 2.730084714528692E-5 3.8221186003401685E-4 54-55 0.24686791031125696 5.460169429057384E-5 4.2316313075194725E-4 56-57 0.2713158189298614 5.460169429057384E-5 4.368135543245907E-4 58-59 0.29738812795361036 5.460169429057384E-5 4.368135543245907E-4 60-61 0.32572640729041824 5.460169429057384E-5 4.914152486151645E-4 62-63 0.3566855679531736 5.460169429057384E-5 5.05065672187808E-4 64-65 0.39075702519049166 5.460169429057384E-5 5.460169429057384E-4 66-67 0.42769507137806484 5.460169429057384E-5 5.460169429057384E-4 68-69 0.4672539988915856 5.460169429057384E-5 6.142690607689557E-4 70-71 0.5121775428691553 5.460169429057384E-5 6.279194843415992E-4 72-73 0.5589302436054591 5.460169429057384E-5 6.279194843415992E-4 74-75 0.6109383574172307 5.460169429057384E-5 6.415699079142425E-4 76-77 0.6681882338808973 6.82521178632173E-5 6.825211786321729E-4 78-79 0.7278951865876399 8.190254143586075E-5 7.507732964953902E-4 80-81 0.7925845438983972 8.190254143586075E-5 7.917245672133206E-4 82-83 0.8631162824982459 8.190254143586075E-5 7.917245672133206E-4 84-85 0.9375929935105887 8.190254143586075E-5 8.190254143586076E-4 86-87 1.0170930603976642 8.190254143586075E-5 8.463262615038945E-4 88-89 1.1009203115572677 8.190254143586075E-5 8.736271086491814E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 9570 0.0 43.02735 3 CGGAAGA 9620 0.0 42.556293 4 GATCGGA 10080 0.0 41.197193 1 ATCGGAA 10075 0.0 40.96493 2 GCCGTCT 5180 0.0 38.793133 50-51 ATGCCGT 5230 0.0 38.372913 48-49 TGCCGTC 5200 0.0 38.2785 50-51 CGTCTTC 5315 0.0 37.942642 52-53 CGTATGC 5300 0.0 37.77235 46-47 TCTCGTA 5225 0.0 37.493874 42-43 GAGCACA 10820 0.0 37.4808 9 TATGCCG 5370 0.0 37.284046 48-49 CCGTCTT 5365 0.0 37.279106 52-53 AGAGCAC 10945 0.0 37.13951 8 CTCGTAT 5290 0.0 37.123207 44-45 TCGTATG 5470 0.0 37.07372 44-45 ATCTCGT 5295 0.0 36.639435 42-43 AATCTCG 5385 0.0 36.161343 40-41 GAAGAGC 11400 0.0 35.782158 6 GTATGCC 5800 0.0 34.84367 46-47 >>END_MODULE