FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_34211-1.3410000003589a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_34211-1.3410000003589a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3662890
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT338640.9245158877279962TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG95700.043.027353
CGGAAGA96200.042.5562934
GATCGGA100800.041.1971931
ATCGGAA100750.040.964932
GCCGTCT51800.038.79313350-51
ATGCCGT52300.038.37291348-49
TGCCGTC52000.038.278550-51
CGTCTTC53150.037.94264252-53
CGTATGC53000.037.7723546-47
TCTCGTA52250.037.49387442-43
GAGCACA108200.037.48089
TATGCCG53700.037.28404648-49
CCGTCTT53650.037.27910652-53
AGAGCAC109450.037.139518
CTCGTAT52900.037.12320744-45
TCGTATG54700.037.0737244-45
ATCTCGT52950.036.63943542-43
AATCTCG53850.036.16134340-41
GAAGAGC114000.035.7821586
GTATGCC58000.034.8436746-47
Produced by FastQC (version 0.11.2)