FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_34139-1.341000000356ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_34139-1.341000000356ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2711023
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1783636.579176938004585TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT53580.19763757076203337TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG269700.074.975373
CGGAAGA268750.074.937254
GATCGGA272900.074.101521
ATCGGAA273450.073.895092
GAAGAGC273150.073.6043556
GGAAGAG293500.068.7921755
GTCGACC353.3882134E-667.9581451
AGAGCAC335400.059.6886068
GAGCACA335150.059.5206079
CGCGGAG407.4819673E-659.463371
AAGAGCA341950.058.5869377
GTCGCGG250.00502657556.9869162
TCGACCG250.00502657556.9869163
TCGCGGT250.00502657556.9869163
CGTCTTC220700.045.0117552-53
GCCGTCT221000.044.98206750-51
ATGCCGT220800.044.8229648-49
TCGTATG225350.044.61256844-45
TGCCGTC221600.044.5708550-51
TATGCCG223050.044.5411848-49
Produced by FastQC (version 0.11.2)