FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_34098-1.34100000035707.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_34098-1.34100000035707.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2286754
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1392606.089854877262705TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT44190.19324334843188204TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC30530.13350802053915725No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA28540.12480572899402384No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG28360.1240185870452178No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC25240.11037479326591318No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC25130.10989376207497614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG202400.074.6630253
GATCGGA202700.074.547851
CGGAAGA202150.074.28554
GAAGAGC202100.073.970036
ATCGGAA204800.073.602562
GGAAGAG209900.071.538015
GAGCACA269850.055.5043149
AGAGCAC271600.055.1291968
AAGAGCA272950.054.7521367
GTGACGC405.354054E-447.5632671
GCCGAAC601.49478E-647.49152
GCGCACA1550.045.9565059
ATCGGAC953.8562575E-1044.9919552
ACCGGGC857.1431714E-944.7654271
GCCGTCT167600.044.6705850-51
CGTCTTC167650.044.64357852-53
ATGCCGT167300.044.61948848-49
TCGTATG170300.044.35421844-45
TCTCGTA164700.044.26196742-43
GTATGCC170350.044.14798446-47
Produced by FastQC (version 0.11.2)