##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_34098-1.34100000035707.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2286754 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66932604031741 33.0 33.0 33.0 33.0 33.0 2 32.6415110676531 33.0 33.0 33.0 33.0 33.0 3 32.71244217786435 33.0 33.0 33.0 33.0 33.0 4 36.65736191999664 37.0 37.0 37.0 37.0 37.0 5 36.66714740632355 37.0 37.0 37.0 37.0 37.0 6 36.55198372890131 37.0 37.0 37.0 37.0 37.0 7 36.43646233919346 37.0 37.0 37.0 37.0 37.0 8 36.382846165350536 37.0 37.0 37.0 37.0 37.0 9 36.42675163135169 37.0 37.0 37.0 37.0 37.0 10-11 36.27510283134959 37.0 37.0 37.0 37.0 37.0 12-13 36.325988497232316 37.0 37.0 37.0 37.0 37.0 14-15 38.22051847290963 40.0 37.0 40.0 37.0 40.0 16-17 38.21549738187842 40.0 37.0 40.0 37.0 40.0 18-19 38.101376667538354 40.0 37.0 40.0 37.0 40.0 20-21 38.14186746803547 40.0 37.0 40.0 37.0 40.0 22-23 37.96787367596164 40.0 37.0 40.0 33.0 40.0 24-25 38.05798021999743 40.0 37.0 40.0 37.0 40.0 26-27 38.005375523558726 40.0 37.0 40.0 35.0 40.0 28-29 37.82338524388719 40.0 37.0 40.0 33.0 40.0 30-31 37.69249578223106 40.0 37.0 40.0 33.0 40.0 32-33 37.64812677708227 40.0 37.0 40.0 33.0 40.0 34-35 37.539902193239854 40.0 37.0 40.0 33.0 40.0 36-37 37.49853285486764 40.0 37.0 40.0 33.0 40.0 38-39 37.43952825708406 40.0 37.0 40.0 33.0 40.0 40-41 37.31289810797314 40.0 37.0 40.0 33.0 40.0 42-43 36.998727016548344 37.0 37.0 40.0 33.0 40.0 44-45 36.848278170717094 37.0 37.0 40.0 33.0 40.0 46-47 37.01774196087555 37.0 37.0 40.0 33.0 40.0 48-49 36.806136995933976 37.0 37.0 40.0 33.0 40.0 50-51 36.70559207505486 37.0 37.0 40.0 33.0 40.0 52-53 36.57692694535573 37.0 37.0 40.0 33.0 40.0 54-55 36.164593786651295 37.0 37.0 40.0 33.0 40.0 56-57 36.082462958411796 37.0 37.0 40.0 33.0 40.0 58-59 35.95143115525325 37.0 37.0 40.0 33.0 40.0 60-61 35.650000174920436 37.0 35.0 40.0 27.0 40.0 62-63 35.32652703351563 37.0 33.0 37.0 27.0 40.0 64-65 35.063609159533556 37.0 33.0 37.0 27.0 40.0 66-67 34.401782395482854 37.0 33.0 37.0 27.0 40.0 68-69 34.193045251041426 37.0 33.0 37.0 27.0 40.0 70-71 33.82066938551326 37.0 33.0 37.0 27.0 37.0 72-73 33.017481110779734 37.0 33.0 37.0 24.5 37.0 74-75 32.29813919643303 37.0 33.0 37.0 22.0 37.0 76-77 29.701350691854042 33.0 27.0 33.0 18.5 37.0 78-79 31.89394661603303 33.0 33.0 37.0 22.0 37.0 80-81 32.223547438858745 37.0 33.0 37.0 22.0 37.0 82-83 32.11121550459735 37.0 33.0 37.0 15.0 37.0 84-85 32.00123471960692 37.0 33.0 37.0 15.0 37.0 86-87 31.882565636706005 37.0 33.0 37.0 15.0 37.0 88-89 31.731195397493565 33.0 33.0 37.0 15.0 37.0 90-91 31.63968446103079 33.0 33.0 37.0 15.0 37.0 92-93 31.474926905124033 33.0 33.0 37.0 15.0 37.0 94-95 31.366831543751537 33.0 33.0 37.0 15.0 37.0 96-97 31.207022705546812 33.0 33.0 37.0 15.0 37.0 98-99 31.066015190090408 33.0 33.0 37.0 15.0 37.0 100-101 29.528271952295697 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16826744424177775 2306 2 0.19842274980607755 2306 3 0.15258098807442622 2306 4 0.15649974174805692 2306 5 0.09542041206352536 2306 6 0.15824374844748945 2306 7 0.2713634206800464 2306 8 0.18912857344392364 2306 9 0.2493662863359205 2306 10-11 0.22671168938083497 2306 12-13 0.2341709645517156 2306 14-15 0.37551738397473855 2306 16-17 0.4046647889471586 2306 18-19 0.5169907980881234 2306 20-21 0.41525804124398036 2306 22-23 0.48127861352934787 2306 24-25 0.45475589272200523 2306 26-27 0.31803047529965056 2306 28-29 0.5046385345088993 2306 30-31 0.6383879587232997 2306 32-33 0.5408749085892097 2306 34-35 0.5893186905920089 2306 36-37 0.5043781737720678 2306 38-39 0.5634078964227669 2306 40-41 0.6505084312912501 2306 42-43 0.6310081531626324 2306 44-45 0.5493597708112148 2306 46-47 0.5632873378898964 2306 48-49 0.4751812556395052 2306 50-51 0.4487181232788515 2306 52-53 0.5981632705026527 2306 54-55 0.7176320626807069 2306 56-57 0.5951874627115004 2306 58-59 0.36536043804227347 2306 60-61 0.41529319632219597 2306 62-63 0.5280363898004552 2306 64-65 0.3995581445951544 2306 66-67 0.4505943289046286 2306 68-69 0.5751403157631145 2306 70-71 0.40509403790691323 2306 72-73 0.34713479529107616 2306 74-75 0.4691746451669161 2306 76-77 0.2914003379996011 2306 78-79 0.5820831887375846 2306 80-81 0.7466898571154772 2306 82-83 0.7103108205921487 2306 84-85 0.6682202173742908 2306 86-87 0.8272550287501872 2306 88-89 0.7707165218432941 2306 90-91 0.706810958419986 2306 92-93 0.6022860124436953 2306 94-95 0.5799052252117036 2306 96-97 0.8324119210870116 2306 98-99 0.5637919833663396 2306 100-101 0.5422414885234552 2307 1 0.16255417980542575 2307 2 0.21646794528260216 2307 3 0.15335676650424546 2307 4 0.11023977937821883 2307 5 0.12790065099018477 2307 6 0.11792029139549953 2307 7 0.1499969769923979 2307 8 0.21794792378022976 2307 9 0.0791663908205038 2307 10-11 0.2211319766741724 2307 12-13 0.18401754372891332 2307 14-15 0.3233397379156244 2307 16-17 0.35991874017982894 2307 18-19 0.415932400630318 2307 20-21 0.3735128147363156 2307 22-23 0.22730184891164384 2307 24-25 0.25699753231825895 2307 26-27 0.3999425301763253 2307 28-29 0.3695242823134848 2307 30-31 0.44411372726767695 2307 32-33 0.5283711278968681 2307 34-35 0.4860553802636147 2307 36-37 0.5183551151494115 2307 38-39 0.5186150390835067 2307 40-41 0.48933915316454346 2307 42-43 0.5614011880471352 2307 44-45 0.2974790809510637 2307 46-47 0.4407531348991256 2307 48-49 0.4345144266158272 2307 50-51 0.4060164307070764 2307 52-53 0.4318010232198475 2307 54-55 0.5096861275632776 2307 56-57 0.43387857720068723 2307 58-59 0.43825054984601763 2307 60-61 0.3496918010994179 2307 62-63 0.4563084726444231 2307 64-65 0.20875593077883536 2307 66-67 0.34878257530495205 2307 68-69 0.1658003362672389 2307 70-71 0.16179895666948596 2307 72-73 0.0479599354876612 2307 74-75 0.16601254610033322 2307 76-77 0.008919117074924543 2307 78-79 0.2683127288089935 2307 80-81 0.5172127972437082 2307 82-83 0.43464417487476936 2307 84-85 0.3715003526019629 2307 86-87 0.491101397125135 2307 88-89 0.5434718707864654 2307 90-91 0.4439135601909925 2307 92-93 0.3589840572201872 2307 94-95 0.4082627746160199 2307 96-97 0.5454090903140774 2307 98-99 0.48649433715010915 2307 100-101 0.23160458425255115 2201 1 0.09166333472672505 2201 2 0.1426008747304195 2201 3 0.13101096932031453 2201 4 0.16142944643137014 2201 5 0.12992027455822353 2201 6 0.11757104644661354 2201 7 0.14774863913964964 2201 8 0.13302672143687744 2201 9 0.1709966460162562 2201 10-11 0.1938873429345236 2201 12-13 0.21994832267449738 2201 14-15 0.3726899894425486 2201 16-17 0.3271861395161437 2201 18-19 0.4127104101928083 2201 20-21 0.4240777801763542 2201 22-23 0.33988219288853116 2201 24-25 0.2936900062659973 2201 26-27 0.2782094991299715 2201 28-29 0.31219490081805645 2201 30-31 0.3936684105601316 2201 32-33 0.3810785405915098 2201 34-35 0.358315218505588 2201 36-37 0.2316682267690382 2201 38-39 0.40417675901492345 2201 40-41 0.5182415245024643 2201 42-43 0.33844591727058315 2201 44-45 0.5204852097883546 2201 46-47 0.45448510527563 2201 48-49 0.6276974474317711 2201 50-51 0.5498068672750165 2201 52-53 0.5700255888978845 2201 54-55 0.6736683901531464 2201 56-57 0.7278769732609263 2201 58-59 0.5457292129786921 2201 60-61 0.5483128127103427 2201 62-63 0.5059033132264474 2201 64-65 0.4769360706179242 2201 66-67 0.5561920361977499 2201 68-69 0.38749679654620195 2201 70-71 0.2462606726028227 2201 72-73 0.16715711895061958 2201 74-75 0.08612305802052589 2201 76-77 0.23022987922853133 2201 78-79 0.31980548638596673 2201 80-81 0.3662992924001429 2201 82-83 0.31461650573790934 2201 84-85 0.37724643153367765 2201 86-87 0.37775377257874254 2201 88-89 0.2743853991482119 2201 90-91 0.1850880697781747 2201 92-93 0.2108239431099861 2201 94-95 0.2947105419177518 2201 96-97 0.2659793238260697 2201 98-99 0.20171212724095966 2201 100-101 0.2063222485211469 1101 1 0.11411462241735393 1101 2 0.13037416736471386 1101 3 0.1340331802232626 1101 4 0.08030782362120448 1101 5 0.08238560762464431 1101 6 0.014620276486994044 1101 7 0.048130340977316166 1101 8 0.10555514268375532 1101 9 0.05264290650914916 1101 10-11 0.13609545083408392 1101 12-13 0.032098301172062804 1101 14-15 0.2828001239662754 1101 16-17 0.32950055089187913 1101 18-19 0.20931672257211886 1101 20-21 0.23407603192366366 1101 22-23 0.3176451201110311 1101 24-25 0.2892041733180122 1101 26-27 0.3622049471979665 1101 28-29 0.27709481604938446 1101 30-31 0.28505106260550406 1101 32-33 0.19125310146441876 1101 34-35 0.2780797277888567 1101 36-37 0.24832977637921516 1101 38-39 0.2803071203906242 1101 40-41 0.4650324556034988 1101 42-43 0.29304665466630553 1101 44-45 0.29295659698909304 1101 46-47 0.3299856370358256 1101 48-49 0.29384498916724766 1101 50-51 0.33770118863731824 1101 52-53 0.24052204409971978 1101 54-55 0.46745294714645524 1101 56-57 0.3441697710533802 1101 58-59 0.45343361150691663 1101 60-61 0.41483628273294215 1101 62-63 0.2860022621704559 1101 64-65 0.22928528517223867 1101 66-67 0.4372624254915394 1101 68-69 0.3013044315542146 1101 70-71 0.08315530638434865 1101 72-73 -0.20649412298327974 1101 74-75 0.12969070217987877 1101 76-77 -0.04443370353477505 1101 78-79 0.3190510128285595 1101 80-81 0.29896350539775796 1101 82-83 0.23805517849036306 1101 84-85 0.37434036612204835 1101 86-87 0.26538604489175555 1101 88-89 0.14904728222140307 1101 90-91 0.1974738195762349 1101 92-93 0.16682948787685703 1101 94-95 0.1812548776599705 1101 96-97 0.02484118434464122 1101 98-99 0.1790564720481882 1101 100-101 0.13671265385137588 2304 1 0.06252715779213958 2304 2 0.13584691078985855 2304 3 0.05103716256975588 2304 4 0.05491006256248454 2304 5 0.01859794760932232 2304 6 0.11647161123855199 2304 7 0.0768557859006549 2304 8 0.10169777499830701 2304 9 0.12565390439853275 2304 10-11 0.051018216142828976 2304 12-13 0.051494004016731765 2304 14-15 0.12936095555757277 2304 16-17 0.09062212747497966 2304 18-19 0.26141554243262277 2304 20-21 0.1670945236213086 2304 22-23 0.1480855301891495 2304 24-25 0.13510492980886113 2304 26-27 0.08138543062487003 2304 28-29 0.13241080701449448 2304 30-31 0.31647573002778984 2304 32-33 0.3892911309422047 2304 34-35 0.3011409017187745 2304 36-37 0.23678104534468503 2304 38-39 0.2533253513057119 2304 40-41 0.3186762399127119 2304 42-43 0.15759607037806234 2304 44-45 0.18171401483827765 2304 46-47 0.2403627901218286 2304 48-49 0.3070356662399618 2304 50-51 0.23812702687488496 2304 52-53 0.21675405102570977 2304 54-55 0.4965842041749724 2304 56-57 0.2849690548362176 2304 58-59 0.21405871714230074 2304 60-61 0.20382493598537366 2304 62-63 0.1589485072583372 2304 64-65 -0.051385634546342374 2304 66-67 0.07134759166292781 2304 68-69 0.18188004262802338 2304 70-71 -0.040314041455054905 2304 72-73 -0.21310434580721704 2304 74-75 -0.08500346015333093 2304 76-77 -0.10055890770168574 2304 78-79 0.2874885734881758 2304 80-81 0.3182711326233587 2304 82-83 0.2780903447724583 2304 84-85 0.33380196330697487 2304 86-87 0.36637925741059973 2304 88-89 0.4372350131892624 2304 90-91 0.2923185912180486 2304 92-93 0.237707358237909 2304 94-95 0.19525398066637578 2304 96-97 0.27811111471323713 2304 98-99 0.26939068950000333 2304 100-101 0.32050285280632806 2202 1 0.06864213058797475 2202 2 0.17114097788675764 2202 3 0.0611436024457106 2202 4 0.08731738307141512 2202 5 0.14808249656110917 2202 6 0.05455208419009239 2202 7 0.16251108642194367 2202 8 0.13775327380392355 2202 9 0.13762310429368796 2202 10-11 0.17170163671929828 2202 12-13 0.16799542997682693 2202 14-15 0.12902987862809567 2202 16-17 0.1990641821148813 2202 18-19 0.19942941555101612 2202 20-21 0.1658720944257439 2202 22-23 0.1896423561292906 2202 24-25 0.13602963852787298 2202 26-27 0.05526472730348786 2202 28-29 0.0963781488986939 2202 30-31 0.09056332927453781 2202 32-33 0.08048082106189014 2202 34-35 0.17178751712429374 2202 36-37 0.1288146876454661 2202 38-39 0.11258723971514684 2202 40-41 0.21188199404783603 2202 42-43 0.1693308705208949 2202 44-45 0.14614766862724338 2202 46-47 0.0805992885038691 2202 48-49 0.15338894658139424 2202 50-51 0.1740708776749713 2202 52-53 0.23946923011141763 2202 54-55 0.2979092129437646 2202 56-57 0.23089632239092595 2202 58-59 0.10368707650460607 2202 60-61 0.08766962562676639 2202 62-63 0.03054120212729572 2202 64-65 0.1303812533734856 2202 66-67 0.1959876529212039 2202 68-69 0.17778929769814766 2202 70-71 0.18646404405641448 2202 72-73 -0.04184507978033736 2202 74-75 -0.03415069960720274 2202 76-77 -0.24467009165676146 2202 78-79 0.13366862641978372 2202 80-81 0.17627497392985703 2202 82-83 0.19720784383904544 2202 84-85 0.10118355864096173 2202 86-87 0.2160797164632342 2202 88-89 0.2004303171434323 2202 90-91 0.032219105314485574 2202 92-93 -0.12373457224814643 2202 94-95 0.1776111308086321 2202 96-97 0.30687172687086317 2202 98-99 0.2681959631212898 2202 100-101 0.2820780281877724 1102 1 0.0727056071274319 1102 2 0.10945896540849986 1102 3 0.07068395731894839 1102 4 0.078842763177839 1102 5 0.05515400954445937 1102 6 0.03434644388047303 1102 7 0.02796831868524663 1102 8 0.044155833798434685 1102 9 0.1274146543386223 1102 10-11 0.16497725435872468 1102 12-13 0.10347910722363451 1102 14-15 0.10014028911515283 1102 16-17 0.16277846905475002 1102 18-19 0.2800673123061941 1102 20-21 0.23763730243602765 1102 22-23 0.09803386628155408 1102 24-25 0.05436611394561908 1102 26-27 0.1605912574342554 1102 28-29 0.2725703715427201 1102 30-31 0.2575555716504283 1102 32-33 0.24944078039708728 1102 34-35 0.15525532738100623 1102 36-37 0.1644222252164269 1102 38-39 0.23846890002258192 1102 40-41 0.30131375934163174 1102 42-43 0.03749265462430884 1102 44-45 0.1593708038055084 1102 46-47 0.07613795758983599 1102 48-49 0.351833990130892 1102 50-51 0.2455169442785845 1102 52-53 0.17177468361178683 1102 54-55 0.29386965056212944 1102 56-57 0.3156798402815113 1102 58-59 0.19683990620362835 1102 60-61 0.30750164743475494 1102 62-63 0.10084046153907167 1102 64-65 0.059467303591077325 1102 66-67 0.24612395289696565 1102 68-69 0.0864362496617872 1102 70-71 -0.015480080460207546 1102 72-73 -0.09368003563030669 1102 74-75 -0.047380483564772646 1102 76-77 -0.2784816009082114 1102 78-79 -0.06816686562205732 1102 80-81 0.10329475512067887 1102 82-83 0.07431909936450865 1102 84-85 0.14574753812840413 1102 86-87 0.11226867906063376 1102 88-89 0.01340937777815654 1102 90-91 -0.04522491089137759 1102 92-93 -0.10439498906226774 1102 94-95 -0.2800969491740375 1102 96-97 -0.17406976172648214 1102 98-99 -0.015000105090894778 1102 100-101 -0.13857801330061292 2305 1 0.04412734802489382 2305 2 0.11929544249377955 2305 3 0.06003225634593434 2305 4 0.10153515362465981 2305 5 0.06694009097121523 2305 6 -0.013602347627809763 2305 7 0.054918520698208795 2305 8 0.07312736072950798 2305 9 0.01874124593775406 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-1.6533357471821155 1213 100-101 -1.6295948073765096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 36.0 9 71.0 10 53.0 11 23.0 12 28.0 13 60.0 14 160.0 15 403.0 16 809.0 17 1374.0 18 1895.0 19 2679.0 20 3755.0 21 5333.0 22 7088.0 23 9984.0 24 13042.0 25 17408.0 26 24166.0 27 35210.0 28 58783.0 29 85128.0 30 65182.0 31 51012.0 32 65319.0 33 92646.0 34 143067.0 35 256999.0 36 506097.0 37 703721.0 38 135174.0 39 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.80748345026407 24.31547559083674 20.343451367822425 14.533589591076765 2 29.94222378095764 27.635766680631146 28.798287878801133 13.623721659610085 3 34.870047434774456 18.117529963235533 30.4951767138857 16.51724588810431 4 29.86435234103298 17.537366958931333 28.917757702561808 23.680522997473883 5 36.47948139590004 16.159018416497794 30.257211750804853 17.10428843679731 6 32.97232671288647 17.100134076511946 33.0063487371182 16.921190473483374 7 31.76568183547509 24.294742678923924 25.497408116483015 18.442167369117975 8 29.140694626531754 30.718389472588658 21.99130295606786 18.14961294481173 9 33.32837725439641 23.322666102256736 24.160272596002894 19.188684047343965 10-11 25.98171906554006 29.386895136074976 27.924778966167764 16.706606832217194 12-13 22.343942549132965 28.431742111307116 27.66462855208737 21.559686787472547 14-15 22.268726762913722 28.60169917708682 27.926943606526983 21.202630453472477 16-17 25.324783514099025 25.398927912665727 28.47446642708398 20.80182214615127 18-19 20.803287104778214 25.48181833288583 32.02865284153871 21.686241720797252 20-21 24.09215162284689 25.78378600574701 31.717002229799196 18.407060141606898 22-23 20.466404285558106 32.576013993659124 28.258051820268943 18.69952990051383 24-25 20.74156205695934 24.708167122480162 32.132336928239766 22.41793389232073 26-27 20.491950598971293 24.8261946846928 28.90660735697849 25.77524735935741 28-29 23.458885389508445 29.167872888819694 28.20565745156672 19.167584270105138 30-31 19.335179910038423 26.904249429540737 31.160107296193644 22.600463364227195 32-33 19.023996459610434 30.52182263592848 28.213200895242775 22.240980009218305 34-35 19.607727809812513 30.45198565302608 31.898074738253435 18.042211798907974 36-37 19.22363697307892 31.05815069884436 32.23296750679297 17.485244821283754 38-39 19.57278472709335 27.19275152664345 31.45705457083433 21.77740917542887 40-41 23.243508829933017 26.430978750127643 27.9216251196359 22.403887300303438 42-43 19.948611547511362 30.071056528828326 31.346584303453817 18.633747620206496 44-45 19.478757193141114 26.870500938677853 31.54819092965623 22.1025509385248 46-47 23.024330215555043 26.594699099082554 28.332228040588188 22.048742644774215 48-49 19.67229388594746 30.769350189772698 31.10507665470028 18.45327926957956 50-51 22.991166015156097 27.201554004680027 30.396390140186654 19.410889839977223 52-53 19.271492795096634 26.788192417083582 27.464049834917244 26.47626495290254 54-55 23.330637397169134 26.78965180431615 30.911394099355384 18.96831669915933 56-57 19.989011943169174 26.76105809913178 30.330295651778332 22.919634305920713 58-59 19.789085372507877 26.858559131305192 30.72254257515646 22.629812921030467 60-61 22.784501984964965 27.11568657084972 30.76895821436047 19.330853229824847 62-63 19.37753306999063 27.169794817914156 30.542950261903172 22.909721850192042 64-65 23.331072970927483 27.103552952943716 30.342183588806765 19.223190487322032 66-67 20.380177974540633 33.654562645292984 26.775485994024358 19.189773386142026 68-69 20.637113630692102 33.56515801645438 26.8447865254008 18.95294182745271 70-71 20.03872595839967 34.430517787454285 26.759208569665716 18.771547684480332 72-73 19.911765143316572 34.385566770396906 26.635391577991456 19.067276508295063 74-75 20.67815132042604 33.100440922161205 26.527183492126266 19.69422426528649 76-77 20.74860147047889 32.11006285181929 26.799302812804797 20.34203286489702 78-79 20.563196596399024 31.726976306128158 27.353582595731197 20.35624450174162 80-81 19.811581937583238 31.71275430205935 27.65013197574358 20.825531784613837 82-83 19.16340817121763 31.46294018115208 27.892072333132305 21.481579314497985 84-85 19.31252659629137 31.173871191072134 28.16720555363365 21.346396659002853 86-87 19.238263841909113 31.39595490204184 28.763891887210047 20.601889368838997 88-89 19.57856994308315 31.576026212323228 28.651785520984795 20.193618323608824 90-91 20.24486518156034 30.99858569286288 27.78207029458572 20.97447883099106 92-93 19.654294596428013 31.506635652816676 27.601349378107848 21.237720372647466 94-95 19.54323660442775 31.533204637596953 27.693050167709917 21.230508590265387 96-97 19.932559461613117 32.184045824218444 27.09251703566986 20.790877678498582 98-99 20.250634526093656 31.4945418944705 27.082781048470707 21.172042530965136 100-101 20.209487159353156 31.51316720498818 26.805589853031336 21.471755782627326 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 59.0 1 31.5 2 22.0 3 51.5 4 57.5 5 42.5 6 36.5 7 39.0 8 41.5 9 43.5 10 41.0 11 43.5 12 54.0 13 56.5 14 56.0 15 58.0 16 60.0 17 77.5 18 95.0 19 113.5 20 158.5 21 205.5 22 255.5 23 352.5 24 472.5 25 653.0 26 1021.5 27 1639.5 28 2350.0 29 3016.0 30 3966.5 31 5476.5 32 9618.0 33 18287.0 34 33001.0 35 54278.5 36 84096.5 37 122505.5 38 154979.5 39 171813.0 40 179000.0 41 170337.5 42 158804.5 43 158750.5 44 151329.0 45 136410.5 46 126358.5 47 110731.5 48 87647.0 49 71345.0 50 59672.0 51 48350.0 52 37953.0 53 29741.5 54 22905.0 55 16221.0 56 11653.5 57 9099.5 58 7245.0 59 5636.0 60 4267.0 61 3286.0 62 2525.5 63 1899.5 64 1483.0 65 1160.5 66 893.5 67 693.5 68 592.0 69 498.0 70 336.5 71 233.5 72 165.5 73 104.0 74 69.0 75 50.5 76 35.5 77 22.0 78 13.5 79 11.5 80 8.5 81 4.5 82 3.0 83 3.0 84 2.5 85 2.5 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15900267365881945 2 0.0 3 0.0015305537893450718 4 0.010582686200614496 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.373010826700205E-5 22-23 1.749204330680082E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 6.340865698715297E-4 38-39 0.0016617441141460777 40-41 0.005619318912309763 42-43 0.0023395607922846095 44-45 0.0011807129232090552 46-47 0.0012900381938765605 48-49 0.003104837686957145 50-51 7.434118405390348E-4 52-53 0.014212285186775666 54-55 0.026106874635400224 56-57 0.027790483803679805 58-59 0.02070620626442547 60-61 0.03032683008316592 62-63 0.00553185869577576 64-65 0.010823201796083006 66-67 0.02800913434501481 68-69 0.02691588163833976 70-71 0.030195639758364916 72-73 0.030436155353833427 74-75 0.03773908343442277 76-77 0.018541565905208866 78-79 0.010254710388611981 80-81 0.015261807785183715 82-83 0.007302928080589343 84-85 0.010976257175017514 86-87 0.03290690647091904 88-89 0.015239942731050215 90-91 0.020793666480959472 92-93 0.048474825013971774 94-95 0.055099936416422586 96-97 0.038854201195231315 98-99 0.03480916618053363 100-101 0.03456865058506512 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2286754.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.035445679711195 #Duplication Level Percentage of deduplicated Percentage of total 1 83.92717324052958 28.564987458797553 2 6.813422160326741 4.637957196614826 3 2.3490067548067177 2.3984847541349614 4 1.3789831160323682 1.877372197558342 5 0.9418446346293732 1.6028050950327732 6 0.6644718602149139 1.3569357542424811 7 0.5204480660023595 1.2399577315673835 8 0.3275803711173715 0.8919475141523945 9 0.27543428811704657 0.8437075876383895 >10 2.143776503611818 14.768208469803119 >50 0.3345831620392815 8.15089236073348 >100 0.2994177187028205 20.73768071221419 >500 0.018932145803846622 4.153041617086429 >1k 0.004796819410224909 2.6541903654505656 >5k 0.0 0.0 >10k+ 1.2915865583944806E-4 6.121831184973095 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 139260 6.089854877262705 TruSeq Adapter, Index 27 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 4419 0.19324334843188204 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3053 0.13350802053915725 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 2854 0.12480572899402384 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2836 0.1240185870452178 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2524 0.11037479326591318 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2513 0.10989376207497614 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004504201151501211 0.0 0.0 2 0.005203882883773244 0.0 0.0 3 0.0059035646160452765 0.0 0.0 4 0.006428325915249301 0.0 0.0 5 0.007084277539254332 0.0 0.0 6 0.008133800137662382 0.0 0.0 7 0.010320305551012484 0.0 0.0 8 0.01171966901555655 0.0 0.0 9 0.013687523887571641 0.0 0.0 10-11 0.02112164229296199 0.0 0.0 12-13 0.027724888641279297 0.0 0.0 14-15 0.03369404841972508 0.0 0.0 16-17 0.03955388292750335 0.0 0.0 18-19 0.04611339916755366 0.0 0.0 20-21 0.05247612992040246 0.0 0.0 22-23 0.05883886067325125 0.0 0.0 24-25 0.0661855188621076 0.0 0.0 26-27 0.07357590715923096 0.0 0.0 28-29 0.08105375567288829 0.0 0.0 30-31 0.13956464053413703 0.0 0.0 32-33 0.15054089770915455 0.0 0.0 34-35 0.15994287098656 0.0 0.0 36-37 0.17179373032691753 0.0 0.0 38-39 0.18456292194088214 0.0 0.0 40-41 0.20002151521326736 0.0 0.0 42-43 0.2180820499275392 0.0 0.0 44-45 0.23806670940555913 0.0 0.0 46-47 0.2605877151630652 0.0 0.0 48-49 0.28498911557605233 0.0 0.0 50-51 0.31203618753919315 0.0 0.0 52-53 0.3408105987788804 0.0 0.0 54-55 0.3734551246001975 0.0 0.0 56-57 0.406121515475648 0.0 0.0 58-59 0.4429859967447307 0.0 0.0 60-61 0.4824086893474331 0.0 0.0 62-63 0.5228590394944099 0.0 0.0 64-65 0.5678135907928881 0.0 0.0 66-67 0.6163540109692605 0.0 0.0 68-69 0.6679992688325898 0.0 0.0 70-71 0.7230336100866119 0.0 0.0 72-73 0.7812383841899916 0.0 0.0 74-75 0.844275335256875 0.0 2.1865054133501025E-5 76-77 0.9123849788827307 0.0 4.373010826700205E-5 78-79 0.9869448134779693 0.0 4.373010826700205E-5 80-81 1.0674738078516535 0.0 4.373010826700205E-5 82-83 1.156049142146466 0.0 4.373010826700205E-5 84-85 1.2519055394677348 0.0 4.373010826700205E-5 86-87 1.355567761114663 0.0 4.373010826700205E-5 88-89 1.4626846613146844 0.0 4.373010826700205E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 20240 0.0 74.663025 3 GATCGGA 20270 0.0 74.54785 1 CGGAAGA 20215 0.0 74.2855 4 GAAGAGC 20210 0.0 73.97003 6 ATCGGAA 20480 0.0 73.60256 2 GGAAGAG 20990 0.0 71.53801 5 GAGCACA 26985 0.0 55.504314 9 AGAGCAC 27160 0.0 55.129196 8 AAGAGCA 27295 0.0 54.752136 7 GTGACGC 40 5.354054E-4 47.563267 1 GCCGAAC 60 1.49478E-6 47.4915 2 GCGCACA 155 0.0 45.956505 9 ATCGGAC 95 3.8562575E-10 44.991955 2 ACCGGGC 85 7.1431714E-9 44.765427 1 GCCGTCT 16760 0.0 44.67058 50-51 CGTCTTC 16765 0.0 44.643578 52-53 ATGCCGT 16730 0.0 44.619488 48-49 TCGTATG 17030 0.0 44.354218 44-45 TCTCGTA 16470 0.0 44.261967 42-43 GTATGCC 17035 0.0 44.147984 46-47 >>END_MODULE