##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_34042-1.3410000003584a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5224388 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.675252106084 33.0 33.0 33.0 33.0 33.0 2 32.64996684779155 33.0 33.0 33.0 33.0 33.0 3 32.72392517554209 33.0 33.0 33.0 33.0 33.0 4 36.6647301081007 37.0 37.0 37.0 37.0 37.0 5 36.675982143745834 37.0 37.0 37.0 37.0 37.0 6 36.56179441496305 37.0 37.0 37.0 37.0 37.0 7 36.44523932755377 37.0 37.0 37.0 37.0 37.0 8 36.40260505153905 37.0 37.0 37.0 37.0 37.0 9 36.42966640303132 37.0 37.0 37.0 37.0 37.0 10-11 36.2860739860822 37.0 37.0 37.0 37.0 37.0 12-13 36.345106929270955 37.0 37.0 37.0 37.0 37.0 14-15 38.25974573481143 40.0 37.0 40.0 37.0 40.0 16-17 38.25193257085806 40.0 37.0 40.0 37.0 40.0 18-19 38.147948333852696 40.0 37.0 40.0 37.0 40.0 20-21 38.18644135925586 40.0 37.0 40.0 37.0 40.0 22-23 37.99233230763106 40.0 37.0 40.0 33.0 40.0 24-25 38.10618612170459 40.0 37.0 40.0 37.0 40.0 26-27 38.07964760657133 40.0 37.0 40.0 37.0 40.0 28-29 37.889899257099586 40.0 37.0 40.0 33.0 40.0 30-31 37.785624555450326 40.0 37.0 40.0 33.0 40.0 32-33 37.6509236105741 40.0 37.0 40.0 33.0 40.0 34-35 37.59475684041844 40.0 37.0 40.0 33.0 40.0 36-37 37.62456119262199 40.0 37.0 40.0 33.0 40.0 38-39 37.44410024676574 40.0 37.0 40.0 33.0 40.0 40-41 37.126114676015646 40.0 37.0 40.0 33.0 40.0 42-43 37.049187388073015 38.5 37.0 40.0 33.0 40.0 44-45 36.96172221511878 37.0 37.0 40.0 33.0 40.0 46-47 37.06952689961006 37.0 37.0 40.0 33.0 40.0 48-49 36.83997513201547 37.0 37.0 40.0 33.0 40.0 50-51 36.75211699437331 37.0 37.0 40.0 33.0 40.0 52-53 36.61070942663523 37.0 37.0 40.0 33.0 40.0 54-55 36.18221311280862 37.0 37.0 40.0 33.0 40.0 56-57 36.10526381271835 37.0 37.0 40.0 33.0 40.0 58-59 35.99883239912503 37.0 37.0 40.0 33.0 40.0 60-61 35.684059262060934 37.0 35.0 40.0 30.0 40.0 62-63 35.46117899359695 37.0 33.0 40.0 27.0 40.0 64-65 35.152756839652795 37.0 33.0 37.0 27.0 40.0 66-67 34.356556882834894 37.0 33.0 37.0 27.0 40.0 68-69 34.170306933558535 37.0 33.0 37.0 27.0 40.0 70-71 33.748502695435334 37.0 33.0 37.0 27.0 37.0 72-73 32.80962277304059 37.0 33.0 37.0 22.0 37.0 74-75 31.9721255389148 35.0 33.0 37.0 18.5 37.0 76-77 29.3940726645877 33.0 27.0 33.0 15.0 37.0 78-79 31.489250224141088 33.0 33.0 37.0 15.0 37.0 80-81 31.792258825339925 37.0 33.0 37.0 15.0 37.0 82-83 31.674143842302676 37.0 33.0 37.0 15.0 37.0 84-85 31.55090060309456 35.0 33.0 37.0 15.0 37.0 86-87 31.429641519733988 33.0 33.0 37.0 15.0 37.0 88-89 31.285759786600842 33.0 33.0 37.0 15.0 37.0 90-91 31.182184592721676 33.0 33.0 37.0 15.0 37.0 92-93 31.011630740289583 33.0 33.0 37.0 15.0 37.0 94-95 30.895566428067745 33.0 33.0 37.0 15.0 37.0 96-97 30.74084505209031 33.0 33.0 37.0 15.0 37.0 98-99 30.61039465292394 33.0 33.0 37.0 15.0 37.0 100-101 29.10641447381014 33.0 27.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10631182971916076 2306 2 0.1825907450622637 2306 3 0.15731488192944454 2306 4 0.16335889081882016 2306 5 0.14767754083213447 2306 6 0.18478125917044252 2306 7 0.188148042270619 2306 8 0.18861714271172048 2306 9 0.15543992037111565 2306 10-11 0.25854971734793253 2306 12-13 0.2250092120949816 2306 14-15 0.3746625385174269 2306 16-17 0.39080702036860515 2306 18-19 0.4969791021618377 2306 20-21 0.44326381483983823 2306 22-23 0.4201301077650683 2306 24-25 0.3808099711313915 2306 26-27 0.4197812759582078 2306 28-29 0.45198738114859793 2306 30-31 0.5029969782245658 2306 32-33 0.48053155849139273 2306 34-35 0.5132569167757666 2306 36-37 0.44638056762770617 2306 38-39 0.5004326178070215 2306 40-41 0.5528062044071334 2306 42-43 0.5535040001237022 2306 44-45 0.5628733339906731 2306 46-47 0.4839748211657948 2306 48-49 0.4216188727092245 2306 50-51 0.5034001370629326 2306 52-53 0.5255347771452108 2306 54-55 0.6820382102827907 2306 56-57 0.5994989092271581 2306 58-59 0.5074077114924407 2306 60-61 0.4175985376340279 2306 62-63 0.3850898715010018 2306 64-65 0.400145087644205 2306 66-67 0.3915740396266685 2306 68-69 0.4359701198718682 2306 70-71 0.3655438234017083 2306 72-73 0.13548339968016165 2306 74-75 0.27521371453884313 2306 76-77 0.04225498173073916 2306 78-79 0.37068641669509717 2306 80-81 0.4365767508891736 2306 82-83 0.3921192966231679 2306 84-85 0.4350844192917833 2306 86-87 0.5554822293462003 2306 88-89 0.4610109301821268 2306 90-91 0.3558138117660654 2306 92-93 0.23685061961021603 2306 94-95 0.35513000731899425 2306 96-97 0.4920583619895318 2306 98-99 0.5293774389346453 2306 100-101 0.3755665666451016 2307 1 0.09704428708566581 2307 2 0.18016552840025923 2307 3 0.10030831217825664 2307 4 0.16473865505328433 2307 5 0.11069415158602425 2307 6 0.15245610308060975 2307 7 0.1878352801794776 2307 8 0.16975648200908466 2307 9 0.1215014477217835 2307 10-11 0.19091535195794052 2307 12-13 0.1331290867571724 2307 14-15 0.2803411798369666 2307 16-17 0.3607078916760358 2307 18-19 0.42683011791060466 2307 20-21 0.4281539695318557 2307 22-23 0.4343386507713092 2307 24-25 0.393246195621181 2307 26-27 0.3853401707490036 2307 28-29 0.3429702811579176 2307 30-31 0.48350700757877263 2307 32-33 0.5340217388100967 2307 34-35 0.4623582353854019 2307 36-37 0.3945331163184065 2307 38-39 0.4190410885925999 2307 40-41 0.5725568101288729 2307 42-43 0.5355134819986418 2307 44-45 0.4310505192483376 2307 46-47 0.37220226967944825 2307 48-49 0.4418645394690799 2307 50-51 0.4125506123215317 2307 52-53 0.4872281188654597 2307 54-55 0.5877148131157028 2307 56-57 0.5658994077827444 2307 58-59 0.5128332892577916 2307 60-61 0.25084305119286654 2307 62-63 0.3051369314208685 2307 64-65 0.30963744623147704 2307 66-67 0.2685973832106683 2307 68-69 0.10722978812272288 2307 70-71 0.14593707659339117 2307 72-73 -0.01105728851292298 2307 74-75 0.04443722586118071 2307 76-77 -0.11341142866903553 2307 78-79 0.33343161751514927 2307 80-81 0.2937731911090964 2307 82-83 0.41592299618519135 2307 84-85 0.4737684888100091 2307 86-87 0.3908019222918746 2307 88-89 0.3760127356988541 2307 90-91 0.4000006303291066 2307 92-93 0.3752091136927831 2307 94-95 0.37204536935421473 2307 96-97 0.4664855472471956 2307 98-99 0.38985293050113157 2307 100-101 0.312008300873039 2201 1 0.13611974017820927 2201 2 0.1590843546818661 2201 3 0.09843357662807506 2201 4 0.11454731881045177 2201 5 0.10667244239254359 2201 6 0.10409813758936082 2201 7 0.17133028155065944 2201 8 0.0798645588301028 2201 9 0.18511532812024 2201 10-11 0.2362913890563192 2201 12-13 0.2058309022056406 2201 14-15 0.3242872454674455 2201 16-17 0.2173905931836586 2201 18-19 0.30953471863818294 2201 20-21 0.3222472464867181 2201 22-23 0.3476182754778918 2201 24-25 0.2802477405483117 2201 26-27 0.19093349809639903 2201 28-29 0.27785550235154943 2201 30-31 0.44124591001008895 2201 32-33 0.4936084415738975 2201 34-35 0.41897533039848867 2201 36-37 0.4076948180499471 2201 38-39 0.42349376126917804 2201 40-41 0.4647098634355942 2201 42-43 0.369948988653789 2201 44-45 0.38184635133419675 2201 46-47 0.4765491550285148 2201 48-49 0.45698008045330596 2201 50-51 0.4997866416383019 2201 52-53 0.5301307778006361 2201 54-55 0.6500405389273212 2201 56-57 0.616245140541281 2201 58-59 0.45177303769173704 2201 60-61 0.45703621251869464 2201 62-63 0.477579598996023 2201 64-65 0.4135305397604654 2201 66-67 0.48248665401069246 2201 68-69 0.4571948167384363 2201 70-71 0.3552045156298931 2201 72-73 0.3182653736396688 2201 74-75 0.37138988484814917 2201 76-77 0.2304738995254958 2201 78-79 0.5289720112399543 2201 80-81 0.4991993277123896 2201 82-83 0.4748428969155931 2201 84-85 0.594361972292873 2201 86-87 0.7470659439596297 2201 88-89 0.565415266691744 2201 90-91 0.6425636983273613 2201 92-93 0.5549207751472878 2201 94-95 0.5959409995295353 2201 96-97 0.501207114793992 2201 98-99 0.5674042986991985 2201 100-101 0.4279637470630604 1101 1 0.09128546768049972 1101 2 0.16242378548405867 1101 3 0.10143049829900974 1101 4 0.09929024490703142 1101 5 0.07964831244481729 1101 6 0.09612373552830178 1101 7 0.08731167521061423 1101 8 0.10481462785691775 1101 9 0.11254459440241504 1101 10-11 0.16899489058418737 1101 12-13 0.14398491089822585 1101 14-15 0.2189727318390453 1101 16-17 0.263494588365937 1101 18-19 0.24520227983360599 1101 20-21 0.3008580695891965 1101 22-23 0.17077059023221608 1101 24-25 0.24005943761394377 1101 26-27 0.2475984573122929 1101 28-29 0.19061509058034432 1101 30-31 0.29362982406940574 1101 32-33 0.31157364360311846 1101 34-35 0.3133610049839817 1101 36-37 0.2914617459689879 1101 38-39 0.3961487865240798 1101 40-41 0.3927765994192711 1101 42-43 0.350416398650772 1101 44-45 0.42269136269600693 1101 46-47 0.41544868927191914 1101 48-49 0.221852678885476 1101 50-51 0.19928734519751146 1101 52-53 0.20888223299925812 1101 54-55 0.48991413086191216 1101 56-57 0.4685512357816819 1101 58-59 0.4580021650031938 1101 60-61 0.3474527618368839 1101 62-63 0.2854141664210843 1101 64-65 0.1428630975195091 1101 66-67 0.3479856640303822 1101 68-69 0.3272340331700434 1101 70-71 0.16808796935534076 1101 72-73 -0.20677092076506653 1101 74-75 -0.09260262992512835 1101 76-77 -0.2536102580382753 1101 78-79 0.21904454289812492 1101 80-81 0.32995067339314943 1101 82-83 0.29893681273330586 1101 84-85 0.18624612738451418 1101 86-87 0.1674421736930114 1101 88-89 0.1460883948235221 1101 90-91 0.15387902642838114 1101 92-93 0.12640413873315737 1101 94-95 0.15509077809479166 1101 96-97 0.23430012908301023 1101 98-99 0.1490307572039562 1101 100-101 0.11586230268995834 2304 1 0.10365156675847231 2304 2 0.15518935532182354 2304 3 0.10030231295335312 2304 4 0.07320501453296657 2304 5 0.07563968062367366 2304 6 0.07889487385809701 2304 7 0.09116723635740698 2304 8 0.0790613998137033 2304 9 0.15102076715904644 2304 10-11 0.13099526030045183 2304 12-13 0.14330213906547584 2304 14-15 0.17671223536326863 2304 16-17 0.22977297513391903 2304 18-19 0.3237865311420194 2304 20-21 0.3000886155755893 2304 22-23 0.21521556147444443 2304 24-25 0.17308230746220232 2304 26-27 0.16584452958583995 2304 28-29 0.25304586605901846 2304 30-31 0.24993925119955662 2304 32-33 0.28603367886744024 2304 34-35 0.22702360728602144 2304 36-37 0.2852064413248243 2304 38-39 0.30580338557049913 2304 40-41 0.41889849611638397 2304 42-43 0.3369952972147843 2304 44-45 0.2776868005074675 2304 46-47 0.24594728182388081 2304 48-49 0.27603206502477917 2304 50-51 0.25183114394351236 2304 52-53 0.2213421380008569 2304 54-55 0.49025253857490014 2304 56-57 0.49164883429016726 2304 58-59 0.3774301500100492 2304 60-61 0.4970988016165805 2304 62-63 0.310645086433901 2304 64-65 0.24562928275285856 2304 66-67 0.2797842378690447 2304 68-69 0.15337849237680246 2304 70-71 0.15496067741083408 2304 72-73 0.07863752291743964 2304 74-75 0.22032833406333197 2304 76-77 0.12109337331054704 2304 78-79 0.2529906438222902 2304 80-81 0.3695200712788491 2304 82-83 0.28752685163373215 2304 84-85 0.2640369194621002 2304 86-87 0.30923099458906833 2304 88-89 0.20815119007721705 2304 90-91 0.16730599447605954 2304 92-93 0.09629438280934721 2304 94-95 0.17325751941109857 2304 96-97 0.22209042309723515 2304 98-99 0.2862316183746607 2304 100-101 0.20019571372596445 2202 1 0.08715723177702728 2202 2 0.1087970799330833 2202 3 0.09495519093179894 2202 4 0.08126273614561796 2202 5 0.06870124552737877 2202 6 0.052990316896043055 2202 7 0.04841136356210285 2202 8 0.020800251335614917 2202 9 0.02062843707380324 2202 10-11 0.14602401419017497 2202 12-13 0.14938914441335527 2202 14-15 0.17710544726819677 2202 16-17 0.1553902193063479 2202 18-19 0.24558293351149274 2202 20-21 0.20645091232503177 2202 22-23 0.17572328288790118 2202 24-25 0.15649952133620815 2202 26-27 0.05803496646127826 2202 28-29 0.16586574238245788 2202 30-31 0.14254486471941874 2202 32-33 0.15289723681284784 2202 34-35 0.19515134365918385 2202 36-37 0.20320383204298054 2202 38-39 0.26401638389418025 2202 40-41 0.3797684473083649 2202 42-43 0.2828950402380954 2202 44-45 0.30208973374768533 2202 46-47 0.23979206734627923 2202 48-49 0.15041790022269197 2202 50-51 0.18469206848892838 2202 52-53 0.22598520398562982 2202 54-55 0.33239459380481406 2202 56-57 0.4630597608651712 2202 58-59 0.4321162410433388 2202 60-61 0.3149890119429628 2202 62-63 0.12497499014543934 2202 64-65 0.15840490658645479 2202 66-67 0.29094305230285755 2202 68-69 0.2596265670973992 2202 70-71 0.14931851187271405 2202 72-73 0.046416380628237164 2202 74-75 0.06701571757081126 2202 76-77 -0.07986404707292394 2202 78-79 0.2992756526830114 2202 80-81 0.22211820386684167 2202 82-83 0.18280957928398678 2202 84-85 0.16354893616649946 2202 86-87 0.22778272580353587 2202 88-89 0.29389057758184123 2202 90-91 0.16604407174090952 2202 92-93 0.09525975205976422 2202 94-95 0.11056073176501258 2202 96-97 0.16368674607064193 2202 98-99 0.1303997862343671 2202 100-101 0.1902906121675798 1102 1 0.11049390068955489 1102 2 0.14660234173809528 1102 3 0.061799114242141684 1102 4 0.090479352473956 1102 5 0.06863707096137261 1102 6 0.013537341895506927 1102 7 -0.00858249300918601 1102 8 0.054992510045117626 1102 9 0.1016775179317122 1102 10-11 0.15270566024931043 1102 12-13 0.07623987771889063 1102 14-15 0.12839867464407462 1102 16-17 0.1420308725504924 1102 18-19 0.21715182785463583 1102 20-21 0.17001299327697694 1102 22-23 0.049102344469964976 1102 24-25 0.10093006545312733 1102 26-27 0.10941259962237382 1102 28-29 0.17611881540776864 1102 30-31 0.22081985638046575 1102 32-33 0.16664684983022937 1102 34-35 0.17359063719160162 1102 36-37 0.10963853836742743 1102 38-39 0.1235165594128631 1102 40-41 0.23085368916082416 1102 42-43 0.1516860947930212 1102 44-45 0.14552798925025456 1102 46-47 0.15700208283357142 1102 48-49 0.18068707032669096 1102 50-51 0.2446463452343295 1102 52-53 0.07935366203161465 1102 54-55 0.17780568034314115 1102 56-57 0.17075902077011307 1102 58-59 0.12741385346482303 1102 60-61 0.18758809035624324 1102 62-63 0.0773806099596257 1102 64-65 0.01087319773363049 1102 66-67 0.14932319436486807 1102 68-69 0.17482806849258736 1102 70-71 0.08677490375686858 1102 72-73 -0.0025206962847050818 1102 74-75 0.051618666035032845 1102 76-77 -0.12182474936541254 1102 78-79 0.17954171893543602 1102 80-81 0.235428932770553 1102 82-83 0.27369377445677756 1102 84-85 0.33291497985045027 1102 86-87 0.41973644380571073 1102 88-89 0.29776787001844696 1102 90-91 0.2762010127645844 1102 92-93 0.1130102871932408 1102 94-95 0.13991035774715144 1102 96-97 0.32535269083586726 1102 98-99 0.44550574493521466 1102 100-101 0.34485690222193455 2305 1 0.051464509625205324 2305 2 0.11813953857070203 2305 3 0.08421270383847457 2305 4 0.052875493423989894 2305 5 0.03991141868820591 2305 6 0.026318468224474145 2305 7 -0.002276098237608437 2305 8 0.07971392895985474 2305 9 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-1.6672949534262997 1213 94-95 -1.7042591083678502 1213 96-97 -1.825898727032687 1213 98-99 -1.8141439757164086 1213 100-101 -1.764002136089541 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 4.0 9 26.0 10 35.0 11 37.0 12 45.0 13 124.0 14 320.0 15 810.0 16 1609.0 17 2746.0 18 4235.0 19 6314.0 20 8654.0 21 11759.0 22 16535.0 23 22960.0 24 30765.0 25 41253.0 26 57250.0 27 83058.0 28 137427.0 29 219524.0 30 193698.0 31 121713.0 32 142243.0 33 203769.0 34 321201.0 35 572586.0 36 1139572.0 37 1603485.0 38 280598.0 39 30.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.19231318557128 24.66957134714894 20.566798033579754 14.571317433700031 2 28.061487776175888 29.69341097942955 28.522517852808786 13.722583391585772 3 32.96890166508464 18.13642366181568 31.896662116773943 16.99801255632574 4 27.251464719807135 17.814622350573313 29.01617307947792 25.91773985014164 5 36.93695797479054 16.80920329807051 29.187399557613254 17.066439169525694 6 32.8177195108786 17.705097707138137 32.562914546163114 16.914268235820156 7 29.703689695328904 26.852504063633866 25.390744332159098 18.05306190887813 8 26.80750357745252 32.67136744054998 22.65312224130367 17.868006740693836 9 34.60185192983369 22.9502288114895 23.78337902927577 18.664540229401034 10-11 26.24824189933826 30.034589697396136 26.788975091436544 16.92819331182906 12-13 21.72823879084019 28.809651963062464 26.431603089203943 23.030506156893402 14-15 21.309682588659186 29.159759956572906 26.926091630254106 22.6044658245138 16-17 25.296723750226818 25.197707367829498 27.57486618528333 21.930702696660358 18-19 20.07410245946511 25.398678275809527 32.0186785514399 22.50854071328546 20-21 24.402123465046753 25.76086699574524 31.74708298229524 18.08992655691277 22-23 19.760142011027906 34.74183458601715 27.252701419478626 18.245321983476327 24-25 19.992810641169836 25.12581377952786 31.823660493822437 23.05771508547987 26-27 20.090793409677843 25.182318005477388 26.97161849387909 27.755270090965677 28-29 24.114212037850173 29.986986035493533 26.86350056695636 19.03530135969993 30-31 19.07251145971547 26.42752605664051 31.07769752170015 23.422264961943867 32-33 18.732835310087996 30.834013476793835 27.264791588986114 23.16835962413205 34-35 19.097002366592985 30.792879472198464 32.27696717778235 17.833150983426194 36-37 18.750939112222405 26.97983040566394 32.33088945627484 21.938341025838817 38-39 24.010009370625315 31.0434336283423 26.840481828729544 18.10607517230284 40-41 19.40286993634104 34.91866905764221 26.81384769934963 18.864613306667117 42-43 19.51581892532432 30.798009939963798 31.3849442347339 18.301226899977976 44-45 19.151588653777356 26.4592926670583 31.493671244670846 22.8954474344935 46-47 23.934484563176188 26.23572878109061 26.969919181919348 22.859867473813846 48-49 19.915281246393008 30.996049750518118 30.823698893635367 18.26497010945351 50-51 24.168637408134416 26.395347167922484 30.515490458612515 18.920524965330582 52-53 19.2879822926446 26.203836363942667 26.575582472750554 27.93259887066218 54-55 24.048568019984135 26.452595787470777 30.805381180686826 18.693455011858255 56-57 19.640327902029554 26.01065454708027 30.830110549996693 23.51890700089348 58-59 19.51454016978688 25.924452458180287 31.37120078786136 23.189806584171478 60-61 23.75462986054581 26.47582803288954 30.817081293022675 18.952460813541975 62-63 19.2409591010244 26.617981215638086 30.721019846654325 23.42003983668319 64-65 24.079556446603277 26.335485209750043 30.812502285208144 18.77245605843854 66-67 19.975649340547648 34.92427946278057 26.14867936731557 18.951391829356215 68-69 19.884114644287617 34.99222322070573 26.242884608333593 18.880777526673057 70-71 19.504333102737455 35.58580129323888 26.37509786602756 18.534767737996106 72-73 19.47338450509624 35.80528319603452 26.02255129175257 18.698781007116665 74-75 20.403530834966162 34.57395743523779 25.746927466033735 19.275584263762312 76-77 20.813764132708855 33.19595646007615 26.29621943337448 19.694059973840513 78-79 20.480931689931346 32.54433842962534 27.155433651913803 19.81929622852951 80-81 19.917161747952015 32.7230718248671 26.94443800991394 20.415328417266945 82-83 19.09179521125391 32.5504623087528 27.304614205441887 21.05312827455141 84-85 19.339400057084912 31.927360500417606 27.61523849414516 21.118000948352318 86-87 19.2542289864219 31.89431440099507 28.111213573196736 20.74024303938629 88-89 19.526938395129136 32.314444128779904 27.75809660407064 20.40052087202032 90-91 20.008397083650344 31.525870331108536 27.44814427514559 21.01758831009553 92-93 19.644656616171616 31.907394931140153 26.99747556669525 21.450472885992976 94-95 19.351200154747474 32.22228286995275 26.874771374015832 21.551745601283944 96-97 19.98270851155806 32.54652592837628 26.60244752114641 20.868318038919252 98-99 20.336609502684656 31.537248435172614 26.6973080315966 21.428834030546135 100-101 20.24422975162951 31.55929807953106 26.356771052487215 21.839701116352213 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 59.0 1 38.5 2 27.5 3 59.0 4 74.0 5 59.0 6 50.5 7 52.0 8 60.5 9 67.5 10 71.5 11 62.5 12 60.0 13 68.5 14 85.5 15 98.5 16 99.5 17 130.0 18 165.0 19 179.0 20 215.5 21 301.5 22 395.0 23 517.0 24 691.5 25 951.0 26 1324.0 27 1827.0 28 2473.5 29 3379.5 30 4895.5 31 7989.0 32 17829.0 33 38936.0 34 73644.5 35 122137.5 36 189995.5 37 281185.0 38 362360.5 39 389734.5 40 394417.0 41 375056.0 42 344861.5 43 355882.5 44 351758.0 45 326625.0 46 326355.0 47 298759.0 48 230232.5 49 178441.5 50 143651.0 51 114709.0 52 86974.0 53 60414.5 54 42532.5 55 29985.0 56 21288.0 57 14384.5 58 8680.0 59 5447.0 60 3726.0 61 2686.0 62 1790.0 63 1023.0 64 621.0 65 439.5 66 344.5 67 275.5 68 221.0 69 159.5 70 98.0 71 63.0 72 42.0 73 31.5 74 21.5 75 14.0 76 12.5 77 11.0 78 9.5 79 7.0 80 5.0 81 4.0 82 3.5 83 1.5 84 1.5 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15712845217468535 2 0.0 3 0.0017035488175839927 4 0.009991600930099372 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.7852494875954847E-5 22-23 9.570498975190969E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.464989200648956E-4 38-39 0.001598273328856892 40-41 0.005742299385114582 42-43 0.00236391324687217 44-45 0.0011293188790725344 46-47 0.0011867418729236802 48-49 0.003081700670011492 50-51 8.230629118664234E-4 52-53 0.015236234368504024 54-55 0.027658742038301903 56-57 0.029620694328216055 58-59 0.02170589167573312 60-61 0.0312668201519489 62-63 0.0059049978676928285 64-65 0.011006073821469614 66-67 0.029314438361009942 68-69 0.028089414492185497 70-71 0.03229086354229433 72-73 0.032845952482855406 74-75 0.04039707617428109 76-77 0.019983201860198745 78-79 0.011293188790725344 80-81 0.01598273328856892 82-83 0.007464989200648957 84-85 0.011312329788675727 86-87 0.03560225618771041 88-89 0.01607843827832083 90-91 0.021935583651137704 92-93 0.050187696625901446 94-95 0.05700189189623741 96-97 0.04144983106155209 98-99 0.036884703050386 100-101 0.03612863363134591 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5224388.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.555751395361245 #Duplication Level Percentage of deduplicated Percentage of total 1 77.70143063531565 15.972112912034545 2 8.498032866958088 3.4936690192559885 3 3.5796213301472646 2.2074541845611853 4 1.9133851994286992 1.5732428193208015 5 1.3286225446105602 1.3655417362643463 6 0.8719167447790228 1.0753742305878138 7 0.6340302137209929 0.9123077215277541 8 0.5053857198303606 0.8310866572478864 9 0.49418829274051024 0.914257051926474 >10 3.454700393047923 14.591951834110231 >50 0.46666217038736096 6.728027964769377 >100 0.4438522331024207 19.73148354983989 >500 0.07308339649187418 10.431246482279615 >1k 0.034522449116974066 11.536499944440838 >5k 4.7150881919911727E-4 0.6569963543649885 >10k+ 9.430150302440753E-5 7.978747537468098 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 411607 7.878568743362859 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8240 0.15772182311114719 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 7335 0.14039921996605154 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 6887 0.13182405288428042 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6108 0.11691321548093288 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 5323 0.10188753208988305 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004593839508091666 0.0 0.0 2 0.005187210444553505 0.0 0.0 3 0.005799722378965728 0.0 0.0 4 0.006163401340022984 0.0 0.0 5 0.006833336268286352 0.0 0.0 6 0.007828668161706213 0.0 0.0 7 0.009034551032580275 0.0 0.0 8 0.010278715899355102 0.0 0.0 9 0.011618585755881837 0.0 0.0 10-11 0.017906403582582303 0.0 0.0 12-13 0.023246742010738865 0.0 0.0 14-15 0.02863493293377138 0.0 0.0 16-17 0.03307564445825999 0.0 0.0 18-19 0.038492546878218084 0.0 0.0 20-21 0.044828217199794504 0.0 0.0 22-23 0.051221310515222065 0.0 0.0 24-25 0.05784409580605422 0.0 0.0 26-27 0.06583546245033868 0.0 0.0 28-29 0.07459246901263841 0.0 0.0 30-31 0.11622413955471914 0.0 0.0 32-33 0.12762260383417157 0.0 0.0 34-35 0.1378821787355763 0.0 0.0 36-37 0.1490892330355249 0.0 0.0 38-39 0.16101407475861287 0.0 0.0 40-41 0.17427878633822755 0.0 0.0 42-43 0.18821343284610562 0.0 1.9140997950381938E-5 44-45 0.2031816932433043 0.0 1.9140997950381938E-5 46-47 0.21989178445398772 0.0 1.9140997950381938E-5 48-49 0.2364391771820929 0.0 1.9140997950381938E-5 50-51 0.2542690167728737 0.0 1.9140997950381938E-5 52-53 0.27284535528371934 0.0 1.9140997950381938E-5 54-55 0.29265628816236466 0.0 1.9140997950381938E-5 56-57 0.31559677420589743 0.0 1.9140997950381938E-5 58-59 0.33862339474020686 0.0 1.9140997950381938E-5 60-61 0.36336313459107555 0.0 1.9140997950381938E-5 62-63 0.3905337811816427 0.0 1.9140997950381938E-5 64-65 0.4200970525160076 0.0 1.9140997950381938E-5 66-67 0.4511820331874279 0.0 1.9140997950381938E-5 68-69 0.48602821995609824 0.0 1.9140997950381938E-5 70-71 0.5239943893906808 0.0 1.9140997950381938E-5 72-73 0.5639895046080039 0.0 1.9140997950381938E-5 74-75 0.6067217825322315 0.0 1.9140997950381938E-5 76-77 0.6536650800055432 0.0 1.9140997950381938E-5 78-79 0.7059008634121355 0.0 1.9140997950381938E-5 80-81 0.7626634928339933 0.0 1.9140997950381938E-5 82-83 0.8221632849627554 0.0 1.9140997950381938E-5 84-85 0.888946226811638 0.0 1.9140997950381938E-5 86-87 0.9605335591460664 0.0 1.9140997950381938E-5 88-89 1.0384661323010467 0.0 1.9140997950381938E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 54745 0.0 81.88994 3 GATCGGA 54880 0.0 81.78147 1 CGGAAGA 54490 0.0 81.64395 4 GAAGAGC 54485 0.0 81.53357 6 ATCGGAA 55555 0.0 80.57626 2 GGAAGAG 55735 0.0 79.8413 5 TCGCCGG 150 0.0 66.50021 2 CGGCGAG 55 1.1697921E-8 60.549217 1 AAGAGCA 77445 0.0 57.336906 7 GAGCACA 77870 0.0 57.13375 9 GTGCGCA 25 0.0049912618 57.089256 1 TCGGACG 150 0.0 57.000187 3 ATCGGAC 150 0.0 57.000187 2 AGAGCAC 78240 0.0 56.875706 8 CGGCGCC 60 2.3246685E-8 55.50345 1 ACGGCGA 70 1.2787496E-9 54.370728 1 GCGCACA 370 0.0 53.909286 9 ACATGCG 45 1.5027343E-5 52.860428 1 GCGGACC 65 4.3692125E-8 51.23395 1 GTACCCC 80 4.1709427E-9 47.574383 1 >>END_MODULE