FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_34042-1.3410000003584a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_34042-1.3410000003584a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5224388
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT4116077.878568743362859TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC82400.15772182311114719No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG73350.14039921996605154No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC68870.13182405288428042No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG61080.11691321548093288No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT53230.10188753208988305TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG547450.081.889943
GATCGGA548800.081.781471
CGGAAGA544900.081.643954
GAAGAGC544850.081.533576
ATCGGAA555550.080.576262
GGAAGAG557350.079.84135
TCGCCGG1500.066.500212
CGGCGAG551.1697921E-860.5492171
AAGAGCA774450.057.3369067
GAGCACA778700.057.133759
GTGCGCA250.004991261857.0892561
TCGGACG1500.057.0001873
ATCGGAC1500.057.0001872
AGAGCAC782400.056.8757068
CGGCGCC602.3246685E-855.503451
ACGGCGA701.2787496E-954.3707281
GCGCACA3700.053.9092869
ACATGCG451.5027343E-552.8604281
GCGGACC654.3692125E-851.233951
GTACCCC804.1709427E-947.5743831
Produced by FastQC (version 0.11.2)