##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_34012-1.3410000003579b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2801229 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66620258465124 33.0 33.0 33.0 33.0 33.0 2 32.63516620740396 33.0 33.0 33.0 33.0 33.0 3 32.711898955779766 33.0 33.0 33.0 33.0 33.0 4 36.65028028768801 37.0 37.0 37.0 37.0 37.0 5 36.6669547545024 37.0 37.0 37.0 37.0 37.0 6 36.55246750622673 37.0 37.0 37.0 37.0 37.0 7 36.4226976801968 37.0 37.0 37.0 37.0 37.0 8 36.37830002473914 37.0 37.0 37.0 37.0 37.0 9 36.440918254094896 37.0 37.0 37.0 37.0 37.0 10-11 36.29448003001539 37.0 37.0 37.0 35.0 37.0 12-13 36.33750685859671 37.0 37.0 37.0 37.0 37.0 14-15 38.24933609497832 40.0 37.0 40.0 37.0 40.0 16-17 38.254656795285214 40.0 37.0 40.0 37.0 40.0 18-19 38.121766731673844 40.0 37.0 40.0 37.0 40.0 20-21 38.18551642868184 40.0 37.0 40.0 37.0 40.0 22-23 38.039244560155566 40.0 37.0 40.0 35.0 40.0 24-25 38.092527601277865 40.0 37.0 40.0 37.0 40.0 26-27 37.987223822115226 40.0 37.0 40.0 37.0 40.0 28-29 37.814556218002885 40.0 37.0 40.0 33.0 40.0 30-31 37.72743106686387 40.0 37.0 40.0 33.0 40.0 32-33 37.644134235365975 40.0 37.0 40.0 33.0 40.0 34-35 37.62283840414332 40.0 37.0 40.0 33.0 40.0 36-37 37.61946417090499 40.0 37.0 40.0 33.0 40.0 38-39 37.42416649977564 40.0 37.0 40.0 33.0 40.0 40-41 37.29067491447504 40.0 37.0 40.0 33.0 40.0 42-43 37.128081638452265 40.0 37.0 40.0 33.0 40.0 44-45 37.10372625729635 40.0 37.0 40.0 33.0 40.0 46-47 37.12403948409788 40.0 37.0 40.0 33.0 40.0 48-49 36.80378862277951 37.0 37.0 40.0 33.0 40.0 50-51 36.77577217000109 37.0 37.0 40.0 33.0 40.0 52-53 36.70004183877862 37.0 37.0 40.0 33.0 40.0 54-55 36.218172809149124 37.0 37.0 40.0 33.0 40.0 56-57 36.17050284000344 37.0 37.0 40.0 33.0 40.0 58-59 35.950352505989336 37.0 37.0 40.0 33.0 40.0 60-61 35.65205165304229 37.0 37.0 40.0 30.0 40.0 62-63 35.45707223507968 37.0 35.0 38.5 27.0 40.0 64-65 35.23654581613999 37.0 33.0 37.0 27.0 40.0 66-67 34.45085121566284 37.0 33.0 37.0 27.0 40.0 68-69 34.25513604921268 37.0 33.0 37.0 27.0 40.0 70-71 33.84245914918059 37.0 33.0 37.0 27.0 40.0 72-73 32.86655767879027 37.0 33.0 37.0 22.0 38.5 74-75 31.867603469762738 37.0 33.0 37.0 15.0 37.0 76-77 29.41322255338639 33.0 27.0 35.0 15.0 37.0 78-79 31.369924415319133 35.0 33.0 37.0 15.0 37.0 80-81 31.582451488257476 37.0 33.0 37.0 15.0 37.0 82-83 31.423175863165774 37.0 33.0 37.0 15.0 37.0 84-85 31.280278049384755 37.0 33.0 37.0 15.0 37.0 86-87 31.139423802909366 35.0 33.0 37.0 15.0 37.0 88-89 30.969068576685448 33.0 33.0 37.0 15.0 37.0 90-91 30.858094250773497 33.0 33.0 37.0 15.0 37.0 92-93 30.69159108377073 33.0 33.0 37.0 15.0 37.0 94-95 30.567658874015656 33.0 33.0 37.0 15.0 37.0 96-97 30.414716361996824 33.0 30.0 37.0 15.0 37.0 98-99 30.29171927750284 33.0 27.0 37.0 15.0 37.0 100-101 28.82837961480479 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11019516677509955 2306 2 0.12577455582732 2306 3 0.15881417477407211 2306 4 0.18523366336238922 2306 5 0.14805330721001297 2306 6 0.20821248487959565 2306 7 0.17950396673290925 2306 8 0.14195318104238908 2306 9 0.13334669337697136 2306 10-11 0.2697023982913933 2306 12-13 0.20448252148599977 2306 14-15 0.3028905302467564 2306 16-17 0.41377700996836353 2306 18-19 0.5220205200645864 2306 20-21 0.3965279229181604 2306 22-23 0.3557862185166556 2306 24-25 0.3508938253547882 2306 26-27 0.39554546317440753 2306 28-29 0.4506291561809732 2306 30-31 0.6029045342733355 2306 32-33 0.5401867712897896 2306 34-35 0.517795759561622 2306 36-37 0.4622946926713567 2306 38-39 0.4621847488388937 2306 40-41 0.5789705433777712 2306 42-43 0.452346035522595 2306 44-45 0.5341694369750698 2306 46-47 0.5002220570625013 2306 48-49 0.4641877831487662 2306 50-51 0.473567848156101 2306 52-53 0.5202632567913454 2306 54-55 0.7073596448581867 2306 56-57 0.519799007784961 2306 58-59 0.45845567012666066 2306 60-61 0.5017566453445781 2306 62-63 0.5107920438824323 2306 64-65 0.5522329024101893 2306 66-67 0.41667662094811675 2306 68-69 0.5293587076011477 2306 70-71 0.1609909364755211 2306 72-73 -0.10514289674275545 2306 74-75 0.04148061106335987 2306 76-77 -0.14334999632830758 2306 78-79 0.2811612456999306 2306 80-81 0.4002513410035995 2306 82-83 0.5571177452931586 2306 84-85 0.47959275937201085 2306 86-87 0.5079769293532905 2306 88-89 0.42139907979989744 2306 90-91 0.3274077899556076 2306 92-93 0.11986390693137494 2306 94-95 0.2536142195502009 2306 96-97 0.47744085083096266 2306 98-99 0.3661623154208158 2306 100-101 0.34854750435694726 2307 1 0.12252258891751922 2307 2 0.24950876070825956 2307 3 0.13163480140065786 2307 4 0.15653849655114271 2307 5 0.134349297149555 2307 6 0.12171676528120656 2307 7 0.2085945579192554 2307 8 0.24285499071415018 2307 9 0.1956246874725025 2307 10-11 0.2042680703447033 2307 12-13 0.1533554275189104 2307 14-15 0.3190091172098519 2307 16-17 0.33557410615467376 2307 18-19 0.3832100883929783 2307 20-21 0.36968143230949124 2307 22-23 0.3328455523476279 2307 24-25 0.30556830636029275 2307 26-27 0.3293811468618131 2307 28-29 0.296081103377027 2307 30-31 0.41736139737445654 2307 32-33 0.3944587667706472 2307 34-35 0.4491207002143369 2307 36-37 0.3495671387208148 2307 38-39 0.3096901455746206 2307 40-41 0.46294778630149125 2307 42-43 0.5604976283161847 2307 44-45 0.44702913529585686 2307 46-47 0.26433359632585507 2307 48-49 0.3374004716982739 2307 50-51 0.286866682730178 2307 52-53 0.2986057023700539 2307 54-55 0.48900906214522877 2307 56-57 0.5080872506347092 2307 58-59 0.3465085413245603 2307 60-61 0.21093143233323275 2307 62-63 0.30406209586593747 2307 64-65 0.20427088466988863 2307 66-67 0.2635710601247183 2307 68-69 0.09925736329277157 2307 70-71 -0.03647343834374084 2307 72-73 -0.15441627082901022 2307 74-75 0.18134685271607154 2307 76-77 -0.010610472048039554 2307 78-79 0.29252364406981 2307 80-81 0.4342057192525779 2307 82-83 0.5140006848833458 2307 84-85 0.4144492835379552 2307 86-87 0.40750685854004587 2307 88-89 0.5369315913499193 2307 90-91 0.47370304015567655 2307 92-93 0.46916451688019833 2307 94-95 0.2587930683810775 2307 96-97 0.6245605743028975 2307 98-99 0.5350300432773842 2307 100-101 0.28461787375594483 2201 1 0.14001892053526888 2201 2 0.20660600436691112 2201 3 0.11123279074683268 2201 4 0.14943194430997409 2201 5 0.14167244909565113 2201 6 0.09665766078686744 2201 7 0.13278626267103277 2201 8 0.09741329346928751 2201 9 0.11769843947184455 2201 10-11 0.21297780071611072 2201 12-13 0.17077618946142792 2201 14-15 0.27990476299527245 2201 16-17 0.23524189986046906 2201 18-19 0.2869778622805015 2201 20-21 0.3684881320866822 2201 22-23 0.2706395984581462 2201 24-25 0.26609801475143513 2201 26-27 0.17227289305179028 2201 28-29 0.25734649006354715 2201 30-31 0.2576502436907617 2201 32-33 0.3546852956302615 2201 34-35 0.308911327172396 2201 36-37 0.4198265178683869 2201 38-39 0.3649979393604923 2201 40-41 0.4599526247625505 2201 42-43 0.2879398300261684 2201 44-45 0.2924058726476986 2201 46-47 0.21894604900722925 2201 48-49 0.2986069040288797 2201 50-51 0.3580780396012173 2201 52-53 0.2825394893216284 2201 54-55 0.4544883340339041 2201 56-57 0.2936174094411257 2201 58-59 0.15273157896526612 2201 60-61 0.2303633485599903 2201 62-63 0.2592648928447545 2201 64-65 0.17250900021242188 2201 66-67 0.23003824286720942 2201 68-69 0.23647187507263112 2201 70-71 0.16259680442008317 2201 72-73 -0.06292491579966963 2201 74-75 0.1307687196774161 2201 76-77 0.03231310348860461 2201 78-79 0.29129070730211026 2201 80-81 0.18798880725971756 2201 82-83 0.22413532902524125 2201 84-85 0.33658969699916597 2201 86-87 0.38191226032048675 2201 88-89 0.27585447825164167 2201 90-91 0.40686591632750435 2201 92-93 0.2769810236994843 2201 94-95 0.22537545734369857 2201 96-97 0.1539383606218827 2201 98-99 0.15998385068781573 2201 100-101 0.11285670996292652 1101 1 0.10937224774084342 1101 2 0.14135088557146958 1101 3 0.13194303490185888 1101 4 0.11591813366062098 1101 5 0.12134046837956447 1101 6 0.12694183875880327 1101 7 0.09427086690901376 1101 8 0.1470871856460576 1101 9 0.1524697056055686 1101 10-11 0.17154805292779685 1101 12-13 0.12403571833549876 1101 14-15 0.2661708481300309 1101 16-17 0.2581550747412109 1101 18-19 0.3319089763460923 1101 20-21 0.39787964134680465 1101 22-23 0.306284189245126 1101 24-25 0.29334123337541484 1101 26-27 0.2631315693937921 1101 28-29 0.37202714532656955 1101 30-31 0.32926763251422386 1101 32-33 0.3618461727393836 1101 34-35 0.309883664068515 1101 36-37 0.31093826216890363 1101 38-39 0.3771136458009252 1101 40-41 0.4049798357105132 1101 42-43 0.24056357195185285 1101 44-45 0.32430321675429497 1101 46-47 0.27460642307914895 1101 48-49 0.3436550909038516 1101 50-51 0.42657122545031 1101 52-53 0.41621912714217046 1101 54-55 0.45477308591014776 1101 56-57 0.4531474936693698 1101 58-59 0.456701731541294 1101 60-61 0.541689469034786 1101 62-63 0.3088365640485691 1101 64-65 0.3817623910608603 1101 66-67 0.43855573438836615 1101 68-69 0.385544982962422 1101 70-71 0.22745550753205634 1101 72-73 -0.044082427592670115 1101 74-75 0.10865846074389296 1101 76-77 0.04184651562835029 1101 78-79 0.5240637230188412 1101 80-81 0.5548055141455173 1101 82-83 0.5427680946333773 1101 84-85 0.625475792764906 1101 86-87 0.5597644684126841 1101 88-89 0.551604766441443 1101 90-91 0.5636049554430258 1101 92-93 0.6911095390237385 1101 94-95 0.5496165330860485 1101 96-97 0.5712868824332737 1101 98-99 0.40923067005703473 1101 100-101 0.45355160355318347 2304 1 0.1130536242739737 2304 2 0.19244821022932967 2304 3 0.1597569268640271 2304 4 0.11614213409651342 2304 5 0.04891214725778781 2304 6 0.07059614352754551 2304 7 0.09980529209312294 2304 8 0.12865457320061324 2304 9 0.10866399874041122 2304 10-11 0.10148510577827352 2304 12-13 0.1742567169637539 2304 14-15 0.22706057382535505 2304 16-17 0.12579200635902765 2304 18-19 0.2935562170290069 2304 20-21 0.2561463925685956 2304 22-23 0.20857358469115894 2304 24-25 0.15134690300806852 2304 26-27 0.09811665368908962 2304 28-29 0.2284854966329135 2304 30-31 0.2545170726392598 2304 32-33 0.1832668702787572 2304 34-35 0.2070760405870118 2304 36-37 0.10978713200093182 2304 38-39 0.15825941812062894 2304 40-41 0.33905257228449415 2304 42-43 0.1234262268917945 2304 44-45 0.18152715513601692 2304 46-47 0.27808372714309115 2304 48-49 0.16466058042804832 2304 50-51 0.05816593197695141 2304 52-53 0.0879800379561928 2304 54-55 0.35450388178268355 2304 56-57 0.2572351484705777 2304 58-59 0.15752114935205697 2304 60-61 0.3767333282192311 2304 62-63 0.09898170523189975 2304 64-65 0.10585666497404844 2304 66-67 0.17809342574099674 2304 68-69 0.11766265987609614 2304 70-71 0.12944219968634485 2304 72-73 -0.11956931322481523 2304 74-75 0.035907003858174136 2304 76-77 0.004666227061299821 2304 78-79 0.2786702364873719 2304 80-81 0.4149987195665297 2304 82-83 0.17685051771269045 2304 84-85 0.3481395413718431 2304 86-87 0.47866041869669473 2304 88-89 0.3328991156268444 2304 90-91 0.3455806392875971 2304 92-93 0.18446848105555347 2304 94-95 0.3164842956395333 2304 96-97 0.3581761974611304 2304 98-99 0.3746657379306768 2304 100-101 0.4161864137254305 2202 1 0.09078795882290791 2202 2 0.12194516749294593 2202 3 0.0723100919535824 2202 4 0.07483406065288989 2202 5 0.07467814902847891 2202 6 0.03360798141868315 2202 7 0.0033566594353189316 2202 8 0.07811333649596719 2202 9 -0.0025043317988107106 2202 10-11 0.12473920575243369 2202 12-13 0.14657868446622757 2202 14-15 0.1647549138598805 2202 16-17 0.12083933833215355 2202 18-19 0.20128475811085167 2202 20-21 0.08469218568117753 2202 22-23 0.06702220428800842 2202 24-25 0.13784537254385754 2202 26-27 -0.06699925763447112 2202 28-29 0.011737059049828247 2202 30-31 0.12250765382383122 2202 32-33 0.1159157162033182 2202 34-35 0.07172185077213555 2202 36-37 0.0657528171367261 2202 38-39 0.05125590005872027 2202 40-41 0.2884814819651851 2202 42-43 0.19165433089806072 2202 44-45 0.19023438166990303 2202 46-47 0.1796192758012225 2202 48-49 0.0474524904716489 2202 50-51 0.05966700903939426 2202 52-53 0.12300579136347523 2202 54-55 0.26626382700129625 2202 56-57 0.32067081439743106 2202 58-59 0.244349921146366 2202 60-61 0.17592205624067248 2202 62-63 0.039542925199867796 2202 64-65 0.0702769870758857 2202 66-67 0.12090136344396285 2202 68-69 0.0548639488059095 2202 70-71 0.06426499516016548 2202 72-73 0.05854551497230176 2202 74-75 0.016248165369031398 2202 76-77 -0.1880437534685413 2202 78-79 0.24118132663875613 2202 80-81 0.3264368055020306 2202 82-83 0.21669856281403455 2202 84-85 0.2206300885046346 2202 86-87 0.13795744070534255 2202 88-89 0.1354473082237071 2202 90-91 0.073269726951807 2202 92-93 0.07451948409035936 2202 94-95 0.11815273952919725 2202 96-97 0.14118963065480372 2202 98-99 0.45475109128085833 2202 100-101 0.37856526169309745 1102 1 0.07354763025328737 1102 2 0.15341959539089345 1102 3 0.09609032582945076 1102 4 0.10528899986228168 1102 5 0.03686028432476007 1102 6 0.0310299490347532 1102 7 0.05371179728975051 1102 8 0.18398257901848325 1102 9 0.07588718029764152 1102 10-11 0.13965876829804813 1102 12-13 0.09784941776966605 1102 14-15 0.11973859710739276 1102 16-17 0.08479193921814243 1102 18-19 0.1673607133554995 1102 20-21 0.14740895036443646 1102 22-23 0.07501574433405978 1102 24-25 0.03304052786860012 1102 26-27 0.09321899071969852 1102 28-29 0.14143093674891105 1102 30-31 0.17768120341762028 1102 32-33 0.23376329785600802 1102 34-35 0.18232338682497584 1102 36-37 0.23957716210871638 1102 38-39 0.0471707627411746 1102 40-41 0.22938828421542468 1102 42-43 0.03035078996687446 1102 44-45 0.09631293424554599 1102 46-47 -0.014650272016517363 1102 48-49 0.14294503293945837 1102 50-51 0.11432776714081427 1102 52-53 0.08704625168598312 1102 54-55 0.24290533293090277 1102 56-57 0.1973035416384974 1102 58-59 0.25197214443266347 1102 60-61 0.07260182564412077 1102 62-63 0.03371119161928249 1102 64-65 0.02378222309202016 1102 66-67 0.24448164636441305 1102 68-69 0.1116503829026314 1102 70-71 0.07267911419337025 1102 72-73 0.13455800247537297 1102 74-75 0.2560138106606793 1102 76-77 0.008782641798799062 1102 78-79 0.5056285812308801 1102 80-81 0.45836765265272916 1102 82-83 0.5679277968625804 1102 84-85 0.5397759003446936 1102 86-87 0.57139524768737 1102 88-89 0.39806997407199773 1102 90-91 0.47920433805590434 1102 92-93 0.4263093933869193 1102 94-95 0.29078223969533923 1102 96-97 0.48179454759099727 1102 98-99 0.4790678408734834 1102 100-101 0.5434825751532237 2305 1 0.09198862589697399 2305 2 0.10325119517288073 2305 3 0.07187122743685848 2305 4 0.004929567508284549 2305 5 -0.010782849164719721 2305 6 0.03578988330845334 2305 7 -0.03158026828121052 2305 8 -0.03903367980495176 2305 9 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Count 7 2.0 8 4.0 9 15.0 10 29.0 11 18.0 12 31.0 13 61.0 14 229.0 15 495.0 16 1003.0 17 1808.0 18 2612.0 19 3786.0 20 5383.0 21 7440.0 22 10244.0 23 13790.0 24 18311.0 25 24853.0 26 33846.0 27 51720.0 28 91812.0 29 135418.0 30 91182.0 31 58571.0 32 72799.0 33 103572.0 34 159404.0 35 276939.0 36 547665.0 37 849946.0 38 238066.0 39 175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.16560969583768 22.78984521399056 21.407172193938205 13.637372896233558 2 27.687168739149854 30.212381779568897 29.684899021108237 12.415550460173016 3 34.11676759469522 17.215942903878414 33.532547638112774 15.134741863313591 4 26.93652172453891 17.388021254513262 30.177253043741842 25.498203977205986 5 38.46479527378875 16.066555072791264 30.179503353706533 15.289146299713446 6 33.21549220003077 17.043126427721546 34.3663085024466 15.375072869801077 7 29.49237638193807 27.574896590032445 26.141525737453097 16.791201290576385 8 26.46270619074699 34.66792611385931 22.76140222737948 16.107965468014218 9 36.50597648389332 22.947963197582204 23.329081628099665 17.21697869042481 10-11 26.56414380973494 30.83064611997091 26.82599316228698 15.779216908007164 12-13 21.222774003839028 29.242628860403773 26.753078737939667 22.78151839781753 14-15 20.533433717843135 29.787853831300477 27.44711339201472 22.231599058841674 16-17 25.08754193248749 25.110656786717545 28.142683086602343 21.65911819419262 18-19 19.21008600153718 25.29161307411854 33.48921134259284 22.009089581751436 20-21 24.395796344353915 25.706667628149578 33.156648948845124 16.740887078651383 22-23 18.919800772805033 36.35312634762518 27.783319006262975 16.94375387330681 24-25 19.283928589915355 25.29018512945568 33.065629407663565 22.360256872965405 26-27 19.319591507870296 25.58239615540179 27.662304652707792 27.435707684020123 28-29 24.30708092769281 31.038661958733115 27.54067946604865 17.113577647525425 30-31 18.61604317247894 26.416190893354308 32.455879187313855 22.5118867468529 32-33 18.226249978134597 31.80113086077575 27.576324534695306 22.396294626394344 34-35 23.709950168301127 26.387007274307102 27.9762025882211 21.926839969170675 36-37 18.343182064017405 26.400833640641334 33.42530559148253 21.83067870385873 38-39 18.913923928623063 31.5272465046043 27.46086031447405 22.09796925229858 40-41 18.954776876097572 26.05239360572239 37.62835733668634 17.364472181493696 42-43 18.573257657663447 31.261337918569225 32.609384418515944 17.556020005251387 44-45 18.32679029714797 26.082224848877107 32.89627612766971 22.69470872630522 46-47 23.78502659402619 26.11794331915009 27.459657367414525 22.637372719409193 48-49 18.86049628713733 31.660354059355466 32.06090519201018 17.418244461497025 50-51 24.200670032955454 26.333626718925117 31.749045187866436 17.71665806025299 52-53 18.535238543407605 26.203321109492926 26.779832698457696 28.481607648641766 54-55 23.933665704689407 26.405454489620002 31.84492883725016 17.815950968440433 56-57 18.77449738459481 26.218202834461106 31.917727531859878 23.089572249084203 58-59 18.661959635553817 26.13116391901402 32.46210767351524 22.74476877191692 60-61 23.529459027612745 26.398505475465527 32.39672605853078 17.675309438390947 62-63 18.29297027285229 26.536962938527907 31.968464975602235 23.201601813017568 64-65 23.551048547585403 26.43525953239683 31.911224232591984 18.102467687425783 66-67 18.540622294496927 36.632384388592236 26.526745122573292 18.30024819433754 68-69 18.461626359264514 36.949730535100066 26.45878396654832 18.129859139087095 70-71 18.102498735428867 37.30542879094144 26.62598494156012 17.966087532069576 72-73 18.069629643913512 36.78537287540633 26.59692917943735 18.548068301242804 74-75 18.572850438602913 35.90303131163752 26.447757452214017 19.07636079754555 76-77 18.8999776305005 34.942175825694285 26.813888799129217 19.343957744675997 78-79 19.171634222914612 34.11325439154108 27.098820407333363 19.616290978210944 80-81 18.859823728619908 34.16388918146455 26.98415985461271 19.992127235302835 82-83 18.553854304749247 33.58396307618928 27.202160220006277 20.660022399055197 84-85 18.81631643916681 32.80662497199579 27.644709278298983 20.732349310538424 86-87 18.83508902995091 32.68299023788994 27.9316371965444 20.550283535614746 88-89 19.25804042151025 32.93928918525014 27.45331132046504 20.34935907277457 90-91 19.630063194472836 32.51838284849484 27.10340723845135 20.74814671858098 92-93 19.30370326188417 32.48883564037538 27.35350701166608 20.85395408607437 94-95 18.94264607262801 32.520317072234214 27.377635459798494 21.159401395339284 96-97 19.17396565803061 33.19090397258814 26.949255232354968 20.685875137026283 98-99 19.542472316500394 32.46499208634738 26.88311720776624 21.109418389385993 100-101 19.670115205233873 32.30921141910279 26.48959724593068 21.53107612973266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 151.0 1 108.5 2 77.0 3 150.0 4 175.0 5 115.0 6 92.0 7 96.5 8 104.5 9 104.0 10 98.0 11 103.5 12 101.0 13 91.5 14 111.5 15 142.5 16 157.5 17 183.5 18 236.0 19 313.5 20 464.0 21 694.0 22 1032.5 23 1673.5 24 2707.5 25 4111.0 26 6623.0 27 10563.0 28 14805.5 29 19170.0 30 23765.5 31 28037.5 32 34358.5 33 45421.0 34 61938.0 35 85925.0 36 113614.0 37 145366.0 38 182487.5 39 204095.5 40 206772.0 41 197640.5 42 184311.5 43 177970.5 44 166281.0 45 151573.0 46 145160.5 47 129799.0 48 100663.5 49 76866.0 50 61900.5 51 49330.0 52 38893.0 53 31107.5 54 25146.0 55 19680.5 56 14007.5 57 9772.0 58 6917.5 59 5021.0 60 3805.5 61 2809.5 62 1892.0 63 1289.0 64 913.0 65 628.5 66 441.0 67 313.0 68 239.0 69 177.0 70 126.5 71 82.0 72 58.0 73 41.5 74 30.5 75 25.0 76 16.0 77 13.0 78 7.5 79 4.5 80 4.5 81 3.0 82 1.0 83 1.5 84 2.5 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1570739129146528 2 0.0 3 0.0020705197611476964 4 0.011352160069740817 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.7849308285756E-5 22-23 1.7849308285756003E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.853695645732641E-4 38-39 0.0015707391291465281 40-41 0.005783175884584945 42-43 0.0023025607688625244 44-45 0.001142355730288384 46-47 0.0012851501965744322 48-49 0.0031593275665788125 50-51 8.38917489430532E-4 52-53 0.014922021726892018 54-55 0.02666686657891947 56-57 0.02864813979863838 58-59 0.021276375476621152 60-61 0.031182741575215736 62-63 0.005461888335441336 64-65 0.010959475287454185 66-67 0.02864813979863838 68-69 0.02754148268492151 70-71 0.031147042958644226 72-73 0.03105779641721544 74-75 0.038840094829805064 76-77 0.019295102256902237 78-79 0.010870228746025406 80-81 0.015493199592036211 82-83 0.007782298412589617 84-85 0.011120119062025988 86-87 0.03439561706665182 88-89 0.015814487141179816 90-91 0.021008635852334815 92-93 0.04853226922897057 94-95 0.05538640361070087 96-97 0.04025019018437979 98-99 0.03587710965436956 100-101 0.036019904120655616 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2801229.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.19064222699117 #Duplication Level Percentage of deduplicated Percentage of total 1 90.18855554045413 44.36432968559602 2 4.569435518779474 4.495469355671737 3 1.3416213423628234 1.979856463687958 4 0.7938581811666826 1.5620157507496089 5 0.5231958730286367 1.2868170502394982 6 0.40531769220024677 1.1962702551175242 7 0.26402042423875366 0.9091133960542871 8 0.2182753114350787 0.8589682201430425 9 0.19202201447492273 0.850111759236784 >10 1.2610401098441995 12.048225641719315 >50 0.12410663065820705 4.231203253801281 >100 0.1038007406410934 10.69076414882416 >500 0.011717478928309454 3.8011487983369245 >1k 0.002959862352922122 2.2682254426863753 >5k 0.0 0.0 >10k+ 7.32794346101255E-5 9.457480778135391 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 262368 9.366174632634463 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3199 0.11419987441226691 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004248135372009928 0.0 0.0 2 0.004605121537725049 0.0 0.0 3 0.005390491102298313 0.0 0.0 4 0.005925970350870993 0.0 0.0 5 0.007211120547445425 0.0 0.0 6 0.008032188728590201 0.0 0.0 7 0.009353037541736145 0.0 0.0 8 0.010281201572595457 0.0 0.0 9 0.011494954536026865 0.0 0.0 10-11 0.01795640413547054 0.0 0.0 12-13 0.023275498004625826 0.0 0.0 14-15 0.028505345332352335 0.0 0.0 16-17 0.03228939868893261 0.0 0.0 18-19 0.03742999947523033 0.0 0.0 20-21 0.0432845725929583 0.0 0.0 22-23 0.04878215954497116 0.0 0.0 24-25 0.05565414323498721 0.0 0.0 26-27 0.06277601724100386 0.0 0.0 28-29 0.07043337049559319 0.0 0.0 30-31 0.13585108536288892 0.0 1.7849308285756E-5 32-33 0.1470604509663437 0.0 3.5698616571512E-5 34-35 0.15544962586064903 0.0 3.5698616571512E-5 36-37 0.16557018365867268 0.0 3.5698616571512E-5 38-39 0.176226220705269 0.0 3.5698616571512E-5 40-41 0.1871142987595802 0.0 3.5698616571512E-5 42-43 0.19905548600275094 0.0 3.5698616571512E-5 44-45 0.2117641935022092 0.0 3.5698616571512E-5 46-47 0.22561525673195587 0.0 3.5698616571512E-5 48-49 0.2412155521737066 0.0 3.5698616571512E-5 50-51 0.2562982176751704 0.0 3.5698616571512E-5 52-53 0.27296947161406654 0.0 3.5698616571512E-5 54-55 0.29196113563011095 0.0 3.5698616571512E-5 56-57 0.3134517028061612 0.0 3.5698616571512E-5 58-59 0.33679859804393 0.0 3.5698616571512E-5 60-61 0.3620732185765605 0.0 3.5698616571512E-5 62-63 0.38770482527490613 0.0 3.5698616571512E-5 64-65 0.41406825361296773 0.0 3.5698616571512E-5 66-67 0.4432697219684646 0.0 3.5698616571512E-5 68-69 0.4775760924936876 0.0 5.354792485726801E-5 70-71 0.5144527634120595 0.0 7.1397233143024E-5 72-73 0.5528644748430064 0.0 7.1397233143024E-5 74-75 0.5960598008945359 0.0 7.1397233143024E-5 76-77 0.6415933863314995 0.0 7.1397233143024E-5 78-79 0.6927138052619046 0.0 1.0709584971453601E-4 80-81 0.7476718254737473 0.0 1.0709584971453601E-4 82-83 0.8080024874796028 0.0 1.0709584971453601E-4 84-85 0.8732774078806125 0.0 1.0709584971453601E-4 86-87 0.9446389424070649 0.0 1.0709584971453601E-4 88-89 1.0230688030146768 0.0 1.0709584971453601E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 33535 0.0 83.54247 1 TCGGAAG 33530 0.0 83.488525 3 CGGAAGA 33500 0.0 82.996185 4 ATCGGAA 33920 0.0 82.43059 2 GAAGAGC 33875 0.0 81.99559 6 GGAAGAG 35330 0.0 78.780045 5 AGAGCAC 40845 0.0 67.79417 8 GAGCACA 40970 0.0 67.72642 9 AAGAGCA 41755 0.0 66.282555 7 TCGGACG 130 0.0 54.802208 3 ATCGGAC 130 0.0 54.802208 2 GCCGTCT 30345 0.0 45.515198 50-51 ATGCCGT 30265 0.0 45.38898 48-49 CGTCTTC 30455 0.0 45.359413 52-53 TCGTATG 30710 0.0 45.336163 44-45 TCTCGTA 30570 0.0 45.256374 42-43 TGCCGTC 30360 0.0 45.195427 50-51 TATGCCG 30475 0.0 45.130764 48-49 GTATGCC 30745 0.0 45.086555 46-47 CGTATGC 30620 0.0 45.07671 46-47 >>END_MODULE