FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_34012-1.3410000003579b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_34012-1.3410000003579b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2801229
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2623689.366174632634463TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC31990.11419987441226691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA335350.083.542471
TCGGAAG335300.083.4885253
CGGAAGA335000.082.9961854
ATCGGAA339200.082.430592
GAAGAGC338750.081.995596
GGAAGAG353300.078.7800455
AGAGCAC408450.067.794178
GAGCACA409700.067.726429
AAGAGCA417550.066.2825557
TCGGACG1300.054.8022083
ATCGGAC1300.054.8022082
GCCGTCT303450.045.51519850-51
ATGCCGT302650.045.3889848-49
CGTCTTC304550.045.35941352-53
TCGTATG307100.045.33616344-45
TCTCGTA305700.045.25637442-43
TGCCGTC303600.045.19542750-51
TATGCCG304750.045.13076448-49
GTATGCC307450.045.08655546-47
CGTATGC306200.045.0767146-47
Produced by FastQC (version 0.11.2)