##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_30749-1.34100000035870.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4775649 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.63861100344686 33.0 33.0 33.0 33.0 33.0 2 32.58363711403413 33.0 33.0 33.0 33.0 33.0 3 32.68464579369213 33.0 33.0 33.0 33.0 33.0 4 36.59501504402857 37.0 37.0 37.0 37.0 37.0 5 36.62820110941989 37.0 37.0 37.0 37.0 37.0 6 36.504249998272485 37.0 37.0 37.0 37.0 37.0 7 36.24827578408715 37.0 37.0 37.0 33.0 37.0 8 36.21914822467062 37.0 37.0 37.0 33.0 37.0 9 36.34950935464478 37.0 37.0 37.0 37.0 37.0 10-11 36.13721998831991 37.0 37.0 37.0 35.0 37.0 12-13 36.25813297836588 37.0 37.0 37.0 35.0 37.0 14-15 38.14209859225416 40.0 37.0 40.0 35.0 40.0 16-17 38.22398610115609 40.0 37.0 40.0 37.0 40.0 18-19 37.990214000233266 40.0 37.0 40.0 37.0 40.0 20-21 38.15524225084381 40.0 37.0 40.0 37.0 40.0 22-23 37.612397812318285 40.0 37.0 40.0 33.0 40.0 24-25 38.02927193769894 40.0 37.0 40.0 37.0 40.0 26-27 38.11913626818051 40.0 37.0 40.0 37.0 40.0 28-29 37.73067545374461 40.0 37.0 40.0 33.0 40.0 30-31 37.59353377938789 40.0 37.0 40.0 33.0 40.0 32-33 37.34289004489233 40.0 37.0 40.0 33.0 40.0 34-35 37.33273446185011 40.0 37.0 40.0 33.0 40.0 36-37 37.558096292252635 40.0 37.0 40.0 33.0 40.0 38-39 37.21195977761347 40.0 37.0 40.0 33.0 40.0 40-41 36.52727304707695 37.0 37.0 40.0 33.0 40.0 42-43 36.40623578072844 37.0 37.0 40.0 33.0 40.0 44-45 36.38088917338774 37.0 37.0 40.0 33.0 40.0 46-47 36.896023451472246 37.0 37.0 40.0 33.0 40.0 48-49 36.46880968429632 37.0 37.0 40.0 33.0 40.0 50-51 36.645111585880784 37.0 37.0 40.0 33.0 40.0 52-53 36.607352320072096 37.0 37.0 40.0 33.0 40.0 54-55 35.87082289757895 37.0 35.0 40.0 30.0 40.0 56-57 35.92063832580661 37.0 37.0 40.0 30.0 40.0 58-59 36.002888507928446 37.0 37.0 40.0 33.0 40.0 60-61 35.67345893720413 37.0 35.0 40.0 27.0 40.0 62-63 35.539171429893614 37.0 33.0 40.0 27.0 40.0 64-65 35.135133360931675 37.0 33.0 38.5 27.0 40.0 66-67 33.534377317093444 37.0 33.0 37.0 27.0 40.0 68-69 33.496057708596254 37.0 33.0 37.0 27.0 40.0 70-71 32.740902021903196 35.0 33.0 37.0 24.5 37.0 72-73 30.382277989860647 33.0 27.0 37.0 15.0 37.0 74-75 28.521474986959888 33.0 22.0 37.0 10.5 37.0 76-77 26.237200744862115 30.0 22.0 33.0 6.0 37.0 78-79 27.44533528322538 33.0 18.5 37.0 6.0 37.0 80-81 27.431804766221305 33.0 15.0 37.0 6.0 37.0 82-83 27.169038281498494 33.0 15.0 37.0 6.0 37.0 84-85 26.983833296793797 33.0 15.0 37.0 6.0 37.0 86-87 26.82446239244132 33.0 15.0 37.0 6.0 37.0 88-89 26.63944178058312 33.0 15.0 37.0 6.0 37.0 90-91 26.514791183355392 33.0 15.0 37.0 6.0 37.0 92-93 26.34633292773401 33.0 15.0 37.0 6.0 37.0 94-95 26.231680133946192 33.0 15.0 37.0 6.0 37.0 96-97 26.09173768842727 33.0 15.0 37.0 6.0 37.0 98-99 25.97369163856054 33.0 15.0 37.0 6.0 37.0 100-101 24.71394788436085 30.0 15.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.07442106650006508 2306 2 0.12892137835035555 2306 3 0.12548818015593355 2306 4 0.18638800851061177 2306 5 0.15523637807726942 2306 6 0.1542281608755829 2306 7 0.1550304546381227 2306 8 0.07049196867944119 2306 9 0.07373989605771669 2306 10-11 0.19504942079175436 2306 12-13 0.15254474034426124 2306 14-15 0.24317484096813757 2306 16-17 0.31693270163236065 2306 18-19 0.473300717102596 2306 20-21 0.4569067629364696 2306 22-23 0.30171804331984475 2306 24-25 0.35437632374488004 2306 26-27 0.39593105368017234 2306 28-29 0.4170761348142591 2306 30-31 0.538849874615984 2306 32-33 0.5412457595321527 2306 34-35 0.42830236426076596 2306 36-37 0.462532012124818 2306 38-39 0.44408580226361494 2306 40-41 0.508864269624155 2306 42-43 0.3945161257782388 2306 44-45 0.5183228659492372 2306 46-47 0.45708553253097506 2306 48-49 0.3764576917835072 2306 50-51 0.41840828656080475 2306 52-53 0.45006609809360043 2306 54-55 0.4831738723349943 2306 56-57 0.3383061632425779 2306 58-59 0.33841304841691766 2306 60-61 0.2592525005704829 2306 62-63 0.2965290787326751 2306 64-65 0.2514926653194891 2306 66-67 0.26398623790828424 2306 68-69 0.17302100452722158 2306 70-71 -0.13435000472470193 2306 72-73 -0.6378246807799073 2306 74-75 -0.44223167907444605 2306 76-77 -0.30173343945030595 2306 78-79 0.06932666897642648 2306 80-81 0.13919132447801275 2306 82-83 0.07845459405428201 2306 84-85 0.18124651657324975 2306 86-87 0.18247112349650862 2306 88-89 0.14909602462903138 2306 90-91 0.059659113214621584 2306 92-93 0.026326710158734556 2306 94-95 -0.07567388199643332 2306 96-97 0.03675528820436469 2306 98-99 -0.02792507316453552 2306 100-101 -0.15179903991095856 2307 1 0.10871219490569217 2307 2 0.23611257730868118 2307 3 0.1397891865114005 2307 4 0.17658555892776207 2307 5 0.10038344198966342 2307 6 0.11805152803245278 2307 7 0.19697856708889105 2307 8 0.14434551591872236 2307 9 0.17512815696942852 2307 10-11 0.19615961770156076 2307 12-13 0.2099315106979418 2307 14-15 0.289556591412925 2307 16-17 0.3314310381959018 2307 18-19 0.4262275959650097 2307 20-21 0.41062153270216584 2307 22-23 0.39776609122360895 2307 24-25 0.4382060569458588 2307 26-27 0.4574338747871778 2307 28-29 0.32038361789033587 2307 30-31 0.5662673341068043 2307 32-33 0.606496853642362 2307 34-35 0.4999912849131434 2307 36-37 0.5162621679270316 2307 38-39 0.5334259100287895 2307 40-41 0.4930669855169896 2307 42-43 0.5754547712407785 2307 44-45 0.5850908287893972 2307 46-47 0.44225330753564407 2307 48-49 0.5202991160843524 2307 50-51 0.5737982468001945 2307 52-53 0.49421158250637376 2307 54-55 0.6603123383700336 2307 56-57 0.6649858937409903 2307 58-59 0.5137252090768811 2307 60-61 0.3139091074003417 2307 62-63 0.29881081650827923 2307 64-65 0.22267001455706037 2307 66-67 0.0733021970317651 2307 68-69 -0.1905178639470435 2307 70-71 -0.13764827905773558 2307 72-73 -0.8976050379590248 2307 74-75 -0.6134081989531772 2307 76-77 -0.5766628939009166 2307 78-79 -0.05431471969058421 2307 80-81 0.039284184070133676 2307 82-83 -0.005857025234544011 2307 84-85 0.052814150644110214 2307 86-87 0.08013038684854479 2307 88-89 -0.05598339791986362 2307 90-91 -0.08790813420342403 2307 92-93 -0.11410090154231511 2307 94-95 -0.0588856570617331 2307 96-97 0.105917810221694 2307 98-99 -0.01636810064462324 2307 100-101 -0.22098100896328887 2201 1 0.13662155831260492 2201 2 0.18744631003098533 2201 3 0.1288183419066513 2201 4 0.12655642823698088 2201 5 0.13285309666230916 2201 6 0.1559991421979987 2201 7 0.15555045298716408 2201 8 0.11542661452662628 2201 9 0.23191524764720128 2201 10-11 0.22963134754991898 2201 12-13 0.1394086045049434 2201 14-15 0.23796657551970668 2201 16-17 0.18710377701609815 2201 18-19 0.3373519685743389 2201 20-21 0.3353095615322843 2201 22-23 0.28862250265190426 2201 24-25 0.2160810656953629 2201 26-27 0.209267667211968 2201 28-29 0.24210402193198632 2201 30-31 0.357552110798963 2201 32-33 0.44485137106106976 2201 34-35 0.35721886148672155 2201 36-37 0.44473435583748966 2201 38-39 0.3488298435984589 2201 40-41 0.6492642311741506 2201 42-43 0.47834148888100003 2201 44-45 0.43290831729151336 2201 46-47 0.35446351892358763 2201 48-49 0.25110129604819775 2201 50-51 0.33477205079491057 2201 52-53 0.43172020333800987 2201 54-55 0.6757554645563602 2201 56-57 0.45105673999270834 2201 58-59 0.4460429312421397 2201 60-61 0.4687326814375865 2201 62-63 0.4602861985011941 2201 64-65 0.30520971670346597 2201 66-67 0.29210199198463727 2201 68-69 0.2591256710983103 2201 70-71 0.04544017118951871 2201 72-73 -0.5467998522843267 2201 74-75 -0.37547054377820643 2201 76-77 -0.37298828371691073 2201 78-79 0.026535556793639614 2201 80-81 0.00830271654210435 2201 82-83 0.009574906737572775 2201 84-85 0.04734558399762179 2201 86-87 0.019349406410370307 2201 88-89 0.024369057704728192 2201 90-91 -0.0023384027410457975 2201 92-93 -0.07401532113131637 2201 94-95 -0.026400304755366477 2201 96-97 -0.11315154655618542 2201 98-99 -0.2573689817708207 2201 100-101 -0.17321645494078552 1101 1 0.04317264513057495 1101 2 0.08845271322208248 1101 3 0.08065194891266714 1101 4 0.10569076028817648 1101 5 0.08916903116080732 1101 6 0.12534124055521545 1101 7 0.18151889815140265 1101 8 0.0833342743627199 1101 9 0.17491494398298357 1101 10-11 0.1300702548086221 1101 12-13 0.12324574863672666 1101 14-15 0.20172814100245517 1101 16-17 0.2723118151203323 1101 18-19 0.2876527273599834 1101 20-21 0.38936155746843326 1101 22-23 0.15409597514592122 1101 24-25 0.24105721305872407 1101 26-27 0.32929657154649306 1101 28-29 0.21822115946563514 1101 30-31 0.35236263932464595 1101 32-33 0.28519709839310536 1101 34-35 0.365956939854577 1101 36-37 0.39861980507307493 1101 38-39 0.3035344364259842 1101 40-41 0.4561251210977417 1101 42-43 0.41138540397498957 1101 44-45 0.46054433824060226 1101 46-47 0.4173255260315756 1101 48-49 0.1369308148341446 1101 50-51 0.1554445077008353 1101 52-53 0.19686756483338996 1101 54-55 0.35307896441764797 1101 56-57 0.30344922036699984 1101 58-59 0.3109184476587572 1101 60-61 0.30432451736893285 1101 62-63 0.21440487872403224 1101 64-65 0.08776739415959156 1101 66-67 0.25317017772216843 1101 68-69 0.14746815184749806 1101 70-71 -0.14199656543238603 1101 72-73 -0.9944405031754471 1101 74-75 -0.805967287823016 1101 76-77 -0.7426190973870455 1101 78-79 -0.20159640217795172 1101 80-81 -0.18462153235758194 1101 82-83 -0.2092027417017448 1101 84-85 -0.2653008590072332 1101 86-87 -0.22221969406874464 1101 88-89 -0.392579951075291 1101 90-91 -0.40163833488521306 1101 92-93 -0.42680846956688256 1101 94-95 -0.40423356685882084 1101 96-97 -0.4507439110882032 1101 98-99 -0.3805657411862846 1101 100-101 -0.4178189172451887 2304 1 0.11038147179493762 2304 2 0.17262895843281711 2304 3 0.13759027173121297 2304 4 0.10984770437252678 2304 5 0.07625312069499302 2304 6 0.09119020203938533 2304 7 0.09358037367930194 2304 8 0.07251685725673696 2304 9 0.13531767494832536 2304 10-11 -0.004668099698342587 2304 12-13 0.12076915485341289 2304 14-15 0.17914567910057855 2304 16-17 0.149657550147559 2304 18-19 0.3131258210136636 2304 20-21 0.23289337551930345 2304 22-23 0.13725804699220845 2304 24-25 0.0802456295021372 2304 26-27 0.09961914522206428 2304 28-29 0.18979117646215116 2304 30-31 0.2840751821760321 2304 32-33 0.2519013590447301 2304 34-35 0.20683283649272965 2304 36-37 0.2222172325498164 2304 38-39 0.126617935403317 2304 40-41 0.3837814746252377 2304 42-43 0.21865090969330225 2304 44-45 0.28625336655851896 2304 46-47 0.20560657347777322 2304 48-49 0.23003995262114074 2304 50-51 0.2553881887425149 2304 52-53 0.22035905217242657 2304 54-55 0.46377697380894745 2304 56-57 0.5053375619105083 2304 58-59 0.44204209009819095 2304 60-61 0.2838813836726217 2304 62-63 0.2504418101330117 2304 64-65 0.10866561672750663 2304 66-67 -0.04232963502809639 2304 68-69 -0.07773746276374283 2304 70-71 -0.05108890835866475 2304 72-73 -0.7163699676412136 2304 74-75 -0.42355543923602923 2304 76-77 -0.26767736086182836 2304 78-79 0.12599542221060744 2304 80-81 0.17568555720540502 2304 82-83 0.041423320580395995 2304 84-85 -0.05122176760055197 2304 86-87 -0.0556247584538454 2304 88-89 -0.10347370845725123 2304 90-91 -0.08717948661511343 2304 92-93 -0.14810027667084924 2304 94-95 0.014318272509715513 2304 96-97 -0.08685768690706297 2304 98-99 0.016864976412310995 2304 100-101 -0.03607454193910442 2202 1 0.13133163948712223 2202 2 0.16128425196016138 2202 3 0.10732645363101767 2202 4 0.06166062855453447 2202 5 0.07586095865416098 2202 6 0.07013268494006297 2202 7 0.1349221752479508 2202 8 0.05682496193891495 2202 9 0.061635048915107404 2202 10-11 0.11662953412530896 2202 12-13 0.1574760500392003 2202 14-15 0.1690417627618146 2202 16-17 0.14320476804176252 2202 18-19 0.28907182941146914 2202 20-21 0.28389609556578677 2202 22-23 0.19086554164454128 2202 24-25 0.1937256384809274 2202 26-27 0.14402476225008343 2202 28-29 0.2262281796575465 2202 30-31 0.2244398136493473 2202 32-33 0.24189673760653818 2202 34-35 0.25257385109647146 2202 36-37 0.3723489435382774 2202 38-39 0.26605590249867106 2202 40-41 0.4118833839394185 2202 42-43 0.19224024869560452 2202 44-45 0.2699552045705147 2202 46-47 0.19340201541930924 2202 48-49 0.12700822314692317 2202 50-51 0.2134161550456355 2202 52-53 0.1856087418758534 2202 54-55 0.34397107421298756 2202 56-57 0.491213126386171 2202 58-59 0.3594832657920648 2202 60-61 0.2185516110473813 2202 62-63 0.3464409650700304 2202 64-65 0.3594721237124787 2202 66-67 0.22708476273248834 2202 68-69 0.13160543474595698 2202 70-71 0.11280122024935935 2202 72-73 -0.2474943635532192 2202 74-75 -0.13886395324558976 2202 76-77 -0.06552238423310186 2202 78-79 0.41643287434571263 2202 80-81 0.27212678571572013 2202 82-83 0.20800721948973688 2202 84-85 0.21321501112498709 2202 86-87 0.2697353176018247 2202 88-89 0.16519148815193319 2202 90-91 0.1422606591582536 2202 92-93 0.07755656173726422 2202 94-95 0.17410225328453421 2202 96-97 0.312708756170025 2202 98-99 0.3165721752926025 2202 100-101 0.24138848655536194 1102 1 0.11571028991095744 1102 2 0.20469182680216846 1102 3 0.14126104000749962 1102 4 0.11132376682729017 1102 5 0.062327466677828625 1102 6 0.01039400723016115 1102 7 0.0493238335076569 1102 8 0.06869977525901305 1102 9 0.0940918451478936 1102 10-11 0.16279726065736355 1102 12-13 0.12186676626898674 1102 14-15 0.151848605375001 1102 16-17 0.16019627416782356 1102 18-19 0.2402835606476188 1102 20-21 0.18758756433121704 1102 22-23 -0.005154578382629893 1102 24-25 0.09499772339108148 1102 26-27 0.09056863338253862 1102 28-29 0.12886857280172848 1102 30-31 0.16105145143463773 1102 32-33 0.25915396994329143 1102 34-35 0.20940883690633427 1102 36-37 0.23574866673448724 1102 38-39 0.13109187811864587 1102 40-41 0.21780251691663466 1102 42-43 0.073453670524664 1102 44-45 0.06746265205122626 1102 46-47 0.16987991646195866 1102 48-49 0.20956947387023916 1102 50-51 0.20348145727731293 1102 52-53 0.19877312517418488 1102 54-55 0.2678657443600798 1102 56-57 0.2672236129910601 1102 58-59 0.11213153276182197 1102 60-61 0.23457004048163554 1102 62-63 0.10238705881464227 1102 64-65 0.0377662784998094 1102 66-67 0.030279971010578777 1102 68-69 -0.02480860328696366 1102 70-71 -0.07029116252059708 1102 72-73 -0.4035739796358371 1102 74-75 -0.35284274147985073 1102 76-77 -0.40687237688912603 1102 78-79 -0.09829007049955507 1102 80-81 -0.05867154133662922 1102 82-83 -0.12889412332405925 1102 84-85 -0.055558702767548596 1102 86-87 -0.04864978171928058 1102 88-89 -0.04563093108159322 1102 90-91 -0.08759516234668041 1102 92-93 -0.17308424937274225 1102 94-95 -0.16709833383916006 1102 96-97 0.018622356521749595 1102 98-99 0.004945319706695983 1102 100-101 -0.08420787871916957 2305 1 0.03846894399656975 2305 2 0.12539891148446003 2305 3 0.06989815411321842 2305 4 5.692264876770992E-4 2305 5 0.021385317383895597 2305 6 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-1.1746516170311878 1213 100-101 -1.0984090872814924 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 12.0 9 48.0 10 38.0 11 27.0 12 37.0 13 97.0 14 285.0 15 745.0 16 1360.0 17 2390.0 18 4038.0 19 6396.0 20 9866.0 21 14968.0 22 22337.0 23 32042.0 24 46464.0 25 66457.0 26 98957.0 27 161960.0 28 306723.0 29 500533.0 30 345914.0 31 118164.0 32 113055.0 33 158814.0 34 244713.0 35 422101.0 36 777699.0 37 1036519.0 38 282526.0 39 361.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.31238500738532 19.39771920085415 14.587727424012334 12.70216836774819 2 21.737715648700313 45.41453946887638 21.14761784209853 11.700127040324782 3 25.468005980425414 15.520732560233522 45.397627094509986 13.613634364831078 4 21.579963038003843 15.794457794740508 21.14412560796201 41.48145355929364 5 51.80491698615204 14.833921002150701 19.71845083254653 13.64271117915073 6 48.47669918790095 15.667399341953313 22.008254794269845 13.847646675875886 7 22.693627609566782 43.68065994799869 18.563801485410675 15.061910957023853 8 21.07058119221073 46.419240610019706 17.995878675338158 14.5142995224314 9 48.68770715770778 17.18340271657318 18.99484237639743 15.13404774932161 10-11 31.04914117431997 34.80343718728072 20.88425049663407 13.263171141765234 12-13 16.87559115001961 33.824125265487474 20.723717341873325 28.576566242619588 14-15 16.72365368560378 33.932047769842384 20.696349333881113 28.647949210672728 16-17 30.70484242037051 19.674184597737394 21.11819775699596 28.502775224896133 18-19 16.20814260009477 19.875225335865345 35.1678588606491 28.748773203390787 20-21 30.34564740651895 20.118205972928905 34.645755852557784 14.890390767994369 22-23 16.104316445830737 48.455600315099 20.294329013774483 15.145754225295782 24-25 15.992831550224901 19.993701379644946 34.722694234856874 29.290772835273277 26-27 15.979566337475806 20.12095110004944 20.676906950238596 43.222575612236156 28-29 30.09817095016824 34.30962995814809 20.491057864595994 15.10114122708767 30-31 15.772139032830932 20.78254704229729 34.41336455003289 29.031949374838895 32-33 15.560890258057073 34.608981941512035 20.502773549731145 29.32735425069975 34-35 15.6633370668573 34.40796214294644 34.877856391874694 15.05084439832157 36-37 15.520124566390109 20.682181486496166 34.672238455182445 29.125455491931284 38-39 29.749097488043287 34.58487590984094 20.468447679434455 15.197578922681318 40-41 15.706356602133875 48.39844199901578 20.47966117666768 15.415540222182667 42-43 15.534518826261486 34.204399111299104 34.57876750336613 15.682314559073271 44-45 15.50850099061048 20.395948940764498 34.68613986060991 29.409410208015114 46-47 29.735851237507944 20.337464380022094 20.8125160778715 29.114168304598465 48-49 15.570369917438509 34.54872828250868 34.65162912726305 15.229272672789762 50-51 29.772618314602894 20.508733501695964 34.08823950978353 15.630408673917618 52-53 15.884677798777789 20.40673187548424 20.309891451392716 43.39869887434526 54-55 29.533109708012915 20.380166535584387 34.52805666943493 15.558667086967768 56-57 15.665527358886559 20.379383625973034 34.7407149697418 29.2143740453986 58-59 15.745400844306046 20.48223380022335 34.59452491239005 29.177840443080555 60-61 29.809726797051166 20.349144032936465 34.35883976694127 15.482289403071094 62-63 15.678953827577683 20.41917132574876 34.77280882314293 29.12906602353063 64-65 29.77512971553889 20.66133563864275 34.52615504211043 15.03737960370793 66-67 15.717425759869935 48.24793146430104 20.407440214432736 15.627202561396283 68-69 15.661038696774432 48.531194426201665 20.204390618721316 15.603376258302589 70-71 15.682460273706331 48.2530463946807 20.353064659643312 15.71142867196965 72-73 15.634836850197104 47.31787295722371 20.51043748193392 16.536852710645267 74-75 15.844008176711998 45.71602945786498 20.704987419355415 17.734974946067613 76-77 16.191431323132143 44.12413375529313 20.988733438896585 18.695701482678142 78-79 16.42697954436271 42.12624033687992 21.562050665177836 19.88472945357954 80-81 16.73214364438048 40.68071692971074 21.90082648955194 20.68631293635684 82-83 17.05562621518774 39.373564404465995 22.025366476383017 21.545442903963245 84-85 17.32188030126721 38.609303497845545 22.22289120230345 21.845924998583797 86-87 17.550122349769104 37.919295654101006 22.382362194696377 22.148219801433513 88-89 18.041669467794154 37.29194952818541 22.391040329324632 22.275340674695798 90-91 18.28049380078635 36.88371637609244 22.421785291849208 22.41400453127201 92-93 18.561784048367976 36.09834950428145 22.473303977825932 22.86656246952464 94-95 18.818493866396032 35.78529437244992 22.533382764061123 22.862828997092922 96-97 18.915665489920674 35.825877204696724 22.57516222807715 22.683295077305456 98-99 19.18652945088025 35.41339696211128 22.61392868218613 22.786144904822336 100-101 19.310774531343462 35.83459443392494 22.271767849543934 22.58286318518766 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 22.0 1 14.5 2 23.0 3 42.0 4 41.5 5 29.5 6 26.5 7 31.0 8 30.5 9 36.0 10 36.5 11 37.0 12 49.5 13 70.5 14 84.0 15 95.5 16 143.5 17 230.5 18 371.5 19 576.0 20 934.0 21 1455.0 22 2232.0 23 3299.5 24 4794.5 25 6802.0 26 9369.5 27 12562.5 28 16336.0 29 21069.0 30 26476.0 31 32822.5 32 42833.5 33 59046.0 34 84510.5 35 121059.5 36 169375.5 37 226066.0 38 278486.5 39 314551.5 40 333447.5 41 333586.5 42 316867.0 43 291402.5 44 259818.0 45 230715.5 46 208676.0 47 190813.0 48 175102.0 49 156865.5 50 137534.0 51 123381.0 52 111599.0 53 99293.5 54 85933.5 55 71836.0 56 58829.0 57 44775.5 58 31846.0 59 22939.0 60 16687.5 61 12230.5 62 8731.0 63 5878.0 64 3838.5 65 2338.0 66 1453.5 67 953.5 68 649.0 69 458.5 70 302.0 71 204.5 72 153.5 73 123.0 74 96.0 75 63.5 76 44.0 77 29.5 78 24.5 79 22.0 80 12.5 81 8.5 82 8.5 83 6.5 84 5.5 85 3.5 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15512027789311986 2 0.0 3 0.0014448298021902365 4 0.009925352554176406 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.187912470116627E-5 22-23 1.4657693645408196E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.004011810750749E-4 38-39 0.001863621049201899 40-41 0.006260929142824358 42-43 0.002910599166731056 44-45 0.0012249643975091133 46-47 0.001329662209262029 48-49 0.003727242098403798 50-51 9.318105246009495E-4 52-53 0.016406147101681888 54-55 0.027095793681654576 56-57 0.029420085102569306 58-59 0.022551908651578037 60-61 0.031147598996492415 62-63 0.0062504593616490665 64-65 0.012249643975091134 66-67 0.02885471691910356 68-69 0.0274412964604392 70-71 0.03101149184121362 72-73 0.031597799587029955 74-75 0.038685841442702346 76-77 0.01977741664012577 78-79 0.011202665857561979 80-81 0.017076213096900546 82-83 0.008574750782563794 84-85 0.011569108198697182 86-87 0.03415242619380109 88-89 0.016458496007558346 90-91 0.02201794981163817 92-93 0.048694952246281085 94-95 0.05526997482436419 96-97 0.039607182186128 98-99 0.035775242275971286 100-101 0.035817121400672454 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4775649.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.962234430970646 #Duplication Level Percentage of deduplicated Percentage of total 1 90.95562102216319 46.35301681346002 2 4.55241430360149 4.640024099340861 3 1.4753986885398576 2.2556884153354457 4 0.8309165273593546 1.6938145143942183 5 0.4401704242789378 1.121603417584152 6 0.3819425155735131 1.1678786410687216 7 0.24457070565863784 0.8724708745706395 8 0.13915728037787312 0.5673412756314785 9 0.11008427912934851 0.5049126756138841 >10 0.7666480287685844 7.526380295613885 >50 0.06527349515157382 2.2921879917837047 >100 0.0352378877401899 2.987861423793707 >500 0.0011350739420828804 0.3755644596024062 >1k 0.0010904597589559632 1.2079680406343043 >5k 2.544809882059706E-4 0.9402250065672453 >10k+ 8.482696811963724E-5 25.493062055005332 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 1163561 24.36445810820686 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 15862 0.3321433380049497 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT 9483 0.19856986977057986 TruSeq Adapter, Index 27 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACATCTCGTAT 8486 0.1776931261070485 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGCAATCTCGTAT 7629 0.15974792117259873 TruSeq Adapter, Index 13 (97% over 38bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG 6166 0.1291133414536956 TruSeq Adapter, Index 13 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAACTCTCGTAT 6150 0.1287783084560863 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCAGAAATCTCGTAT 5585 0.11694745572800683 TruSeq Adapter, Index 27 (97% over 37bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0051720719005940346 0.0 0.0 2 0.005695560959358613 0.0 0.0 3 0.006407506079278439 0.0 0.0 4 0.006805357763939519 0.0 0.0 5 0.007852335881468676 0.0 0.0 6 0.008564281001388502 0.0 0.0 7 0.010239445989435153 0.0 0.0 8 0.01189367141513122 0.0 0.0 9 0.012919709970309794 0.0 0.0 10-11 0.022133117404566374 0.0 0.0 12-13 0.02812183223683315 0.0 0.0 14-15 0.03345095085505656 0.0 2.0939562350583137E-5 16-17 0.03671752258174753 0.0 2.0939562350583137E-5 18-19 0.04008879212019141 0.0 3.140934352587471E-5 20-21 0.04343912209628471 0.0 4.187912470116627E-5 22-23 0.04738622959936963 0.0 4.187912470116627E-5 24-25 0.05141709535185689 0.0 4.187912470116627E-5 26-27 0.05529091438671477 0.0 4.187912470116627E-5 28-29 0.06049439563083468 0.0 4.187912470116627E-5 30-31 0.10762935048199732 0.0 4.187912470116627E-5 32-33 0.11580624957990003 0.0 4.187912470116627E-5 34-35 0.12203576937919852 0.0 5.234890587645784E-5 36-37 0.12891441561136507 0.0 8.375824940233255E-5 38-39 0.1369761471163396 0.0 8.375824940233255E-5 40-41 0.14494365059073647 0.0 8.375824940233255E-5 42-43 0.15341370356154732 0.0 8.375824940233255E-5 44-45 0.16369502867568364 0.0 8.375824940233255E-5 46-47 0.1748453456273692 0.0 8.375824940233255E-5 48-49 0.1859851927978794 0.0 1.0469781175291568E-4 50-51 0.19875832583173514 0.0 1.256373741034988E-4 52-53 0.2123376320160883 0.0 1.256373741034988E-4 54-55 0.2273617680026317 0.0 1.256373741034988E-4 56-57 0.24401918985252058 0.0 1.3610715527879037E-4 58-59 0.261126812292947 0.0 1.4657693645408194E-4 60-61 0.2799724184084718 0.0 1.4657693645408194E-4 62-63 0.30127842310019015 0.0 1.4657693645408194E-4 64-65 0.32383033175176823 0.0 1.4657693645408194E-4 66-67 0.34795270757964 0.0 1.4657693645408194E-4 68-69 0.3722949488121928 0.0 1.4657693645408194E-4 70-71 0.398909132559784 0.0 1.570467176293735E-4 72-73 0.4289155254081697 0.0 1.675164988046651E-4 74-75 0.4609321162422113 0.0 1.7798627997995666E-4 76-77 0.495681319963004 2.0939562350583137E-5 1.989258423305398E-4 78-79 0.5344090405304075 2.0939562350583137E-5 2.0939562350583135E-4 80-81 0.57705245925737 2.0939562350583137E-5 2.1986540468112292E-4 82-83 0.6225017793393107 2.0939562350583137E-5 2.4080496703170605E-4 84-85 0.674075921408797 2.0939562350583137E-5 2.512747482069976E-4 86-87 0.7275032147463099 2.0939562350583137E-5 2.512747482069976E-4 88-89 0.786291036045572 2.0939562350583137E-5 2.512747482069976E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 139375 0.0 91.57832 1 TCGGAAG 140085 0.0 90.912994 3 ATCGGAA 140060 0.0 90.83088 2 CGGAAGA 140035 0.0 90.34757 4 GAAGAGC 140605 0.0 89.77804 6 GAGCACA 142085 0.0 88.729256 9 GGAAGAG 143030 0.0 88.46839 5 AGAGCAC 143650 0.0 87.80225 8 AAGAGCA 143945 0.0 87.50355 7 TCGGACG 605 0.0 54.168858 3 GCGCACA 905 0.0 50.37393 9 TTGTGTT 24160 0.0 48.950947 1 CGGAAGC 975 0.0 46.76469 4 GCCGTCT 135020 0.0 46.55028 50-51 ATGCCGT 134865 0.0 46.524208 48-49 GTATGCC 136285 0.0 46.45313 46-47 TCTCGTA 134550 0.0 46.33472 42-43 TCGTATG 137230 0.0 46.269295 44-45 CGTCTTC 136360 0.0 46.107258 52-53 TATGCCG 135795 0.0 46.093647 48-49 >>END_MODULE