FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_30749-1.34100000035870.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_30749-1.34100000035870.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4775649
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT116356124.36445810820686TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT158620.3321433380049497TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT94830.19856986977057986TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACATCTCGTAT84860.1776931261070485TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGCAATCTCGTAT76290.15974792117259873TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG61660.1291133414536956TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAACTCTCGTAT61500.1287783084560863TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCAGAAATCTCGTAT55850.11694745572800683TruSeq Adapter, Index 27 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1393750.091.578321
TCGGAAG1400850.090.9129943
ATCGGAA1400600.090.830882
CGGAAGA1400350.090.347574
GAAGAGC1406050.089.778046
GAGCACA1420850.088.7292569
GGAAGAG1430300.088.468395
AGAGCAC1436500.087.802258
AAGAGCA1439450.087.503557
TCGGACG6050.054.1688583
GCGCACA9050.050.373939
TTGTGTT241600.048.9509471
CGGAAGC9750.046.764694
GCCGTCT1350200.046.5502850-51
ATGCCGT1348650.046.52420848-49
GTATGCC1362850.046.4531346-47
TCTCGTA1345500.046.3347242-43
TCGTATG1372300.046.26929544-45
CGTCTTC1363600.046.10725852-53
TATGCCG1357950.046.09364748-49
Produced by FastQC (version 0.11.2)