##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_30521-1.341000000356df.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3043743 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68716051256627 33.0 33.0 33.0 33.0 33.0 2 32.66232990104618 33.0 33.0 33.0 33.0 33.0 3 32.7300780650666 33.0 33.0 33.0 33.0 33.0 4 36.67936616199199 37.0 37.0 37.0 37.0 37.0 5 36.68259639529356 37.0 37.0 37.0 37.0 37.0 6 36.5628858941113 37.0 37.0 37.0 37.0 37.0 7 36.49726208815922 37.0 37.0 37.0 37.0 37.0 8 36.42876681769781 37.0 37.0 37.0 37.0 37.0 9 36.43490366959365 37.0 37.0 37.0 37.0 37.0 10-11 36.300810219522475 37.0 37.0 37.0 37.0 37.0 12-13 36.35848772383214 37.0 37.0 37.0 37.0 37.0 14-15 38.27379512659249 40.0 37.0 40.0 37.0 40.0 16-17 38.22865103919746 40.0 37.0 40.0 37.0 40.0 18-19 38.12699478898186 40.0 37.0 40.0 37.0 40.0 20-21 38.117281255349084 40.0 37.0 40.0 37.0 40.0 22-23 38.02037852735924 40.0 37.0 40.0 35.0 40.0 24-25 38.031035471785884 40.0 37.0 40.0 35.0 40.0 26-27 37.96203785930678 40.0 37.0 40.0 33.0 40.0 28-29 37.814299203316445 40.0 37.0 40.0 33.0 40.0 30-31 37.68508609301114 40.0 37.0 40.0 33.0 40.0 32-33 37.59847250572732 40.0 37.0 40.0 33.0 40.0 34-35 37.49375719303502 40.0 37.0 40.0 33.0 40.0 36-37 37.43629669127781 40.0 37.0 40.0 33.0 40.0 38-39 37.334388284424804 40.0 37.0 40.0 33.0 40.0 40-41 37.17658471822358 40.0 37.0 40.0 33.0 40.0 42-43 37.04703961536832 37.0 37.0 40.0 33.0 40.0 44-45 36.89718169372381 37.0 37.0 40.0 33.0 40.0 46-47 36.851620521180664 37.0 37.0 40.0 33.0 40.0 48-49 36.67671892797782 37.0 37.0 40.0 33.0 40.0 50-51 36.52104415517341 37.0 37.0 40.0 33.0 40.0 52-53 36.32094431100129 37.0 37.0 40.0 33.0 40.0 54-55 35.92842020499103 37.0 37.0 40.0 30.0 40.0 56-57 35.769046203966624 37.0 37.0 40.0 27.0 40.0 58-59 35.57837307551919 37.0 33.0 40.0 27.0 40.0 60-61 35.26857047391977 37.0 33.0 40.0 27.0 40.0 62-63 34.972464330924126 37.0 33.0 37.0 27.0 40.0 64-65 34.72950393643616 37.0 33.0 37.0 27.0 40.0 66-67 34.255929623493174 37.0 33.0 37.0 27.0 40.0 68-69 34.01662853926892 37.0 33.0 37.0 27.0 40.0 70-71 33.72883206630783 37.0 33.0 37.0 27.0 37.0 72-73 33.2785778891319 37.0 33.0 37.0 27.0 37.0 74-75 32.85048540563379 37.0 33.0 37.0 24.5 37.0 76-77 30.0506619645614 33.0 30.0 33.0 22.0 37.0 78-79 32.58663182141199 33.0 33.0 37.0 27.0 37.0 80-81 33.02848072258401 37.0 33.0 37.0 27.0 37.0 82-83 32.94192857281314 37.0 33.0 37.0 27.0 37.0 84-85 32.85390537243124 37.0 33.0 37.0 27.0 37.0 86-87 32.748644350065035 37.0 33.0 37.0 27.0 37.0 88-89 32.594043583837404 33.0 33.0 37.0 24.5 37.0 90-91 32.50972733243247 33.0 33.0 37.0 22.0 37.0 92-93 32.35107957537808 33.0 33.0 37.0 22.0 37.0 94-95 32.22946368993703 33.0 33.0 37.0 22.0 37.0 96-97 32.05530328940387 33.0 33.0 37.0 22.0 37.0 98-99 31.927120818019134 33.0 33.0 37.0 22.0 37.0 100-101 30.17340458770665 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11708913240573082 2306 2 0.15656452681525224 2306 3 0.1154394347923926 2306 4 0.13017487159950747 2306 5 0.11290314650886302 2306 6 0.1567799618219965 2306 7 0.19060279118944834 2306 8 0.16070598855478124 2306 9 0.16282607311154607 2306 10-11 0.1921274235726642 2306 12-13 0.20741715837922214 2306 14-15 0.4098432527588187 2306 16-17 0.4871928493281814 2306 18-19 0.5289097035043042 2306 20-21 0.4671622793307648 2306 22-23 0.34662314511440684 2306 24-25 0.4100664719195777 2306 26-27 0.4651739662560601 2306 28-29 0.46074112298061465 2306 30-31 0.5306811095393229 2306 32-33 0.5072979543560479 2306 34-35 0.4353408299838648 2306 36-37 0.4460319986199863 2306 38-39 0.5574616012887148 2306 40-41 0.49186855634475535 2306 42-43 0.38770363859447343 2306 44-45 0.5718881567263594 2306 46-47 0.4070879174006379 2306 48-49 0.5278751094073968 2306 50-51 0.5431774743645548 2306 52-53 0.5352046453982169 2306 54-55 0.6194286299431937 2306 56-57 0.6854614315293617 2306 58-59 0.5733534158705496 2306 60-61 0.6017007642367247 2306 62-63 0.4881190446717838 2306 64-65 0.4102226931594686 2306 66-67 0.5519578388445581 2306 68-69 0.5717768682010274 2306 70-71 0.4534968048742769 2306 72-73 0.18043008154175055 2306 74-75 0.2772859963672758 2306 76-77 0.0059207229785691595 2306 78-79 0.22013411586090115 2306 80-81 0.24288580528544657 2306 82-83 0.3835956948261341 2306 84-85 0.3165824653976941 2306 86-87 0.5487437131066812 2306 88-89 0.539083445910741 2306 90-91 0.28633917401523945 2306 92-93 0.30059996555602453 2306 94-95 0.3252195848300943 2306 96-97 0.47476719563999126 2306 98-99 0.5153979711199277 2306 100-101 0.4480937774839333 2307 1 0.1348405372112751 2307 2 0.2228547324505854 2307 3 0.165692702601703 2307 4 0.17522683429914565 2307 5 0.11619864911707367 2307 6 0.10048451963553617 2307 7 0.18711343548664416 2307 8 0.23709166054527486 2307 9 0.17785598187471408 2307 10-11 0.2513621318363448 2307 12-13 0.18434738133545636 2307 14-15 0.37815078936432656 2307 16-17 0.41556161330071006 2307 18-19 0.4923558073088614 2307 20-21 0.4617041818100702 2307 22-23 0.43776053408944904 2307 24-25 0.3877154618694263 2307 26-27 0.48595275218182366 2307 28-29 0.4357214859138878 2307 30-31 0.5088952945507046 2307 32-33 0.5579766919444111 2307 34-35 0.5071331906613921 2307 36-37 0.40324962767076045 2307 38-39 0.4013746141182679 2307 40-41 0.5834792489793443 2307 42-43 0.4810602678667948 2307 44-45 0.47600136693360895 2307 46-47 0.36934069176570716 2307 48-49 0.5845708456463399 2307 50-51 0.5227322668158578 2307 52-53 0.6225694517465357 2307 54-55 0.6403169302795462 2307 56-57 0.7518347170623798 2307 58-59 0.5721336519181577 2307 60-61 0.37769721949442214 2307 62-63 0.3467409757507198 2307 64-65 0.36729430299612886 2307 66-67 0.4858640038764861 2307 68-69 0.4645559663058023 2307 70-71 0.34810743708712266 2307 72-73 0.30906797403132913 2307 74-75 0.2664377757876366 2307 76-77 0.09287516498376291 2307 78-79 0.4094807554049211 2307 80-81 0.4742796845628021 2307 82-83 0.472909102411073 2307 84-85 0.38630664013737714 2307 86-87 0.4750921250218454 2307 88-89 0.546723020138856 2307 90-91 0.3672124192670907 2307 92-93 0.2978029423339379 2307 94-95 0.39253444253689196 2307 96-97 0.5242261692759698 2307 98-99 0.4653687672771021 2307 100-101 0.2915070521410392 2201 1 0.14166490640496932 2201 2 0.150810342673104 2201 3 0.11986069093603646 2201 4 0.13080608659982573 2201 5 0.11768342660256792 2201 6 0.12575654410657222 2201 7 0.16168313689787794 2201 8 0.11300687125849862 2201 9 0.1194161571726724 2201 10-11 0.23808601408040175 2201 12-13 0.20553133693655212 2201 14-15 0.278237848279133 2201 16-17 0.22845788947421397 2201 18-19 0.35227791557878874 2201 20-21 0.4054686216745438 2201 22-23 0.38560896779509335 2201 24-25 0.38026370124509157 2201 26-27 0.329886159039539 2201 28-29 0.3779369258393217 2201 30-31 0.4274865701422925 2201 32-33 0.488284816280391 2201 34-35 0.45623273970241485 2201 36-37 0.4252595916051263 2201 38-39 0.43407720906399305 2201 40-41 0.538915223268198 2201 42-43 0.37278316721391036 2201 44-45 0.4482836731912556 2201 46-47 0.39500946103855483 2201 48-49 0.4115162725349535 2201 50-51 0.39668315065700455 2201 52-53 0.41261997558333263 2201 54-55 0.5989393325709429 2201 56-57 0.5687377245314309 2201 58-59 0.4610276662876842 2201 60-61 0.41320424924506227 2201 62-63 0.4175270781421716 2201 64-65 0.5058665530933553 2201 66-67 0.5170212664090741 2201 68-69 0.38389093464935087 2201 70-71 0.33672400719734696 2201 72-73 0.49968559517296995 2201 74-75 0.3569000731323726 2201 76-77 0.13590200833933253 2201 78-79 0.42461785730313295 2201 80-81 0.3403510927590787 2201 82-83 0.48500517333352633 2201 84-85 0.5774709671398526 2201 86-87 0.5776164260484578 2201 88-89 0.6308647033474202 2201 90-91 0.5037655741054721 2201 92-93 0.506165765292188 2201 94-95 0.41406013005128983 2201 96-97 0.2740300788911796 2201 98-99 0.27620307888900797 2201 100-101 0.3997886353255815 1101 1 0.11292883789393215 1101 2 0.15419894643675747 1101 3 0.16026327427190523 1101 4 0.12658741410243834 1101 5 0.0916956954233683 1101 6 0.13048135607575517 1101 7 0.12396056206515738 1101 8 0.09602336128248368 1101 9 0.18309609481628542 1101 10-11 0.19141236268902873 1101 12-13 0.13352413963326626 1101 14-15 0.2860096198685156 1101 16-17 0.2971663227700958 1101 18-19 0.3807318113343072 1101 20-21 0.4102326594527881 1101 22-23 0.29832648123309013 1101 24-25 0.319105603519688 1101 26-27 0.2964278092566772 1101 28-29 0.32931969887626167 1101 30-31 0.3463954346690059 1101 32-33 0.31656687768522573 1101 34-35 0.3381086444267183 1101 36-37 0.33078106710291877 1101 38-39 0.3221609144098352 1101 40-41 0.48268312795200785 1101 42-43 0.40290352718030675 1101 44-45 0.3799016998546847 1101 46-47 0.43789465848356457 1101 48-49 0.40099236756769585 1101 50-51 0.3276153583361534 1101 52-53 0.3402686034392204 1101 54-55 0.4306479581998204 1101 56-57 0.45648231643117043 1101 58-59 0.42426042970797795 1101 60-61 0.3562584972736502 1101 62-63 0.2509464551857121 1101 64-65 0.17428446233242312 1101 66-67 0.343650782438516 1101 68-69 0.349508880330454 1101 70-71 0.31612792674140877 1101 72-73 0.10756416219089715 1101 74-75 0.10652932596929077 1101 76-77 -0.19974944377480952 1101 78-79 0.25284297801739797 1101 80-81 0.4141348396083657 1101 82-83 0.36714173988580967 1101 84-85 0.29259163078992856 1101 86-87 0.2956410649179517 1101 88-89 0.3165622141534996 1101 90-91 0.24398569416175775 1101 92-93 0.1793477123095073 1101 94-95 0.28505530148073177 1101 96-97 0.08204505049675248 1101 98-99 0.2444440923826825 1101 100-101 0.19229675886410647 2304 1 0.1452842607268252 2304 2 0.18566290313460598 2304 3 0.09028400989001284 2304 4 0.12215090176722043 2304 5 0.062283517108078 2304 6 0.10514019725250279 2304 7 0.04630319058048826 2304 8 0.07907654317963164 2304 9 0.12759398678702638 2304 10-11 0.19485014618380347 2304 12-13 0.10115899697937891 2304 14-15 0.1589588755094553 2304 16-17 0.11839193015550364 2304 18-19 0.28925843812901775 2304 20-21 0.27338564529907927 2304 22-23 0.16807074653871013 2304 24-25 0.18849457834741656 2304 26-27 0.17754943523795674 2304 28-29 0.28051034248007056 2304 30-31 0.2997390530895103 2304 32-33 0.22874850184663842 2304 34-35 0.21012624488554366 2304 36-37 0.18468526413540332 2304 38-39 0.27966427153950235 2304 40-41 0.3797252692354718 2304 42-43 0.2675509527375084 2304 44-45 0.187141932468613 2304 46-47 0.2900497013472787 2304 48-49 0.2167819754935536 2304 50-51 0.1369428018715766 2304 52-53 0.2317974003046075 2304 54-55 0.3596513525543301 2304 56-57 0.38836450117118915 2304 58-59 0.22302863196848932 2304 60-61 0.22510240384204394 2304 62-63 0.1419063323161467 2304 64-65 0.23034122057221396 2304 66-67 0.13979249604985 2304 68-69 0.2200097061705648 2304 70-71 0.121910117271959 2304 72-73 0.031607552574755005 2304 74-75 0.15686593508910107 2304 76-77 -0.037564648607975926 2304 78-79 0.21752342339200226 2304 80-81 0.24743556673369937 2304 82-83 0.30173995971093603 2304 84-85 0.2286592669570382 2304 86-87 0.2784972308690712 2304 88-89 0.3026693500186681 2304 90-91 0.29686737981309363 2304 92-93 0.20424624013576675 2304 94-95 0.1462943098108198 2304 96-97 0.2586639177033163 2304 98-99 0.08656697022177084 2304 100-101 0.23894114577088388 2202 1 0.04499002612411118 2202 2 0.08663081749025991 2202 3 0.0552721578774964 2202 4 0.0705280281577032 2202 5 0.08995732080363439 2202 6 0.011971501025520581 2202 7 0.06577134765709758 2202 8 0.08954440072825065 2202 9 0.03496676485744388 2202 10-11 0.14140053918517737 2202 12-13 0.15316473707171951 2202 14-15 0.0790351905922364 2202 16-17 0.13347710197095353 2202 18-19 0.21327664478267394 2202 20-21 0.17814207224007106 2202 22-23 0.09751944720696315 2202 24-25 0.17928867609682442 2202 26-27 0.012145240296881354 2202 28-29 0.13572180665548927 2202 30-31 0.1054020686133228 2202 32-33 0.10950776655707983 2202 34-35 0.07402589268194504 2202 36-37 0.19052315494195682 2202 38-39 0.24261349896386264 2202 40-41 0.3590140710202405 2202 42-43 0.26851822155870764 2202 44-45 0.1697788632363313 2202 46-47 0.2177042681162007 2202 48-49 0.2150078531621915 2202 50-51 0.1769104218635178 2202 52-53 0.30637509166684396 2202 54-55 0.28038959866305646 2202 56-57 0.3123426201354391 2202 58-59 0.23011769764774925 2202 60-61 0.1549313888553172 2202 62-63 0.24790270183039098 2202 64-65 0.2706603986261058 2202 66-67 0.16357482441515003 2202 68-69 0.2673946461100414 2202 70-71 0.18409374083758223 2202 72-73 0.15487655194471017 2202 74-75 0.1742204832666303 2202 76-77 0.05169733767570861 2202 78-79 0.33192703993420736 2202 80-81 0.4577731554301323 2202 82-83 0.41220846279400547 2202 84-85 0.3391235095127314 2202 86-87 0.48180772519352644 2202 88-89 0.42491104586349593 2202 90-91 0.2800841296245977 2202 92-93 0.1572297712608588 2202 94-95 0.33262253567558275 2202 96-97 0.31465331544549713 2202 98-99 0.407312058995668 2202 100-101 0.3452817142543445 1102 1 0.11075245724519078 1102 2 0.15394024117260585 1102 3 0.12208059992626374 1102 4 0.10624584045077512 1102 5 0.048265000005009995 1102 6 0.06294480911131473 1102 7 0.08074101769655329 1102 8 0.15759392191566235 1102 9 0.0647928395235553 1102 10-11 0.1974695807468052 1102 12-13 0.13387730720914703 1102 14-15 0.12425628564612623 1102 16-17 0.15245523012931272 1102 18-19 0.16685246774786577 1102 20-21 0.2132415231448448 1102 22-23 0.21674787256603167 1102 24-25 0.15876791173921845 1102 26-27 0.1474740536625916 1102 28-29 0.20154074947291178 1102 30-31 0.3670008825211539 1102 32-33 0.20605527704139348 1102 34-35 0.28171593655535787 1102 36-37 0.18572934155689325 1102 38-39 0.14014883874074258 1102 40-41 0.28272618519177684 1102 42-43 0.12675155218141754 1102 44-45 0.18464228644625535 1102 46-47 0.19012970655983708 1102 48-49 0.2930524419796967 1102 50-51 0.046761201942750574 1102 52-53 0.09769494351181862 1102 54-55 0.2853687994545524 1102 56-57 0.2334239756804095 1102 58-59 0.2501989534804281 1102 60-61 0.0930795187175022 1102 62-63 0.02862352485366415 1102 64-65 0.017779149182757692 1102 66-67 0.2291766751913542 1102 68-69 0.16237606864618215 1102 70-71 0.05432582360458582 1102 72-73 0.010564678050748455 1102 74-75 0.1165848455145877 1102 76-77 -0.10705490827093911 1102 78-79 0.23180682854416546 1102 80-81 0.3919093178817832 1102 82-83 0.38261714763735455 1102 84-85 0.3284990685791911 1102 86-87 0.3475650980942504 1102 88-89 0.17774086603287031 1102 90-91 0.22287226909014635 1102 92-93 0.20905087580192827 1102 94-95 0.20209697447934616 1102 96-97 0.23718098318479264 1102 98-99 0.25790154738450966 1102 100-101 0.34584828333850126 2305 1 0.07292704839064612 2305 2 0.1305199833260886 2305 3 0.08948202826633178 2305 4 0.03284939738335879 2305 5 0.02240996287741126 2305 6 0.03739522983908472 2305 7 0.012410596577005606 2305 8 0.06609391884057914 2305 9 -0.00703223813358278 2305 10-11 0.14848345709810218 2305 12-13 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-1.7739943659660256 1213 100-101 -1.5247064263281693 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 14.0 10 17.0 11 16.0 12 33.0 13 85.0 14 244.0 15 637.0 16 1309.0 17 2039.0 18 3033.0 19 4168.0 20 5773.0 21 7543.0 22 9788.0 23 12624.0 24 15985.0 25 20617.0 26 26593.0 27 35502.0 28 51139.0 29 70000.0 30 70273.0 31 75282.0 32 99430.0 33 141863.0 34 220118.0 35 387383.0 36 718553.0 37 891780.0 38 171873.0 39 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.95242509288426 25.485908673157624 18.299623198048568 16.262043035909546 2 32.879977054567355 23.812884333532757 28.09353483523412 15.21360377666577 3 38.724599893287355 18.92059895758041 24.149025787137184 18.20577536199505 4 33.27043297744196 18.579185776086124 27.472779139906905 20.67760210656501 5 36.841776720307855 16.92813749386857 27.01949540417834 19.210590381645233 6 32.94814969595002 17.866652999284106 29.71926342007193 19.465933884693946 7 34.91644991052135 20.262453170323514 23.24072696019342 21.58036995896171 8 31.66998659216629 25.824847892874004 21.71694522172207 20.788220293237636 9 31.872434696359054 22.206014108287068 24.37462689852593 21.546924296827953 10-11 24.915096313979202 28.689429429488627 28.564287457909554 17.831186798622618 12-13 23.801697449489 27.48476136125816 28.15705859528876 20.556482593964077 14-15 24.003866292259236 27.560983302466735 27.87871709273746 20.55643331253657 16-17 24.994702246543156 26.134269549038798 28.200869784341187 20.67015842007686 18-19 23.216234090723166 26.102121631162685 29.204551764061552 21.477092514052597 20-21 23.874371127917176 26.483461317200565 29.02871563072178 20.613451924160483 22-23 22.555294308588902 28.988376289595465 27.64466557623026 20.81166382558537 24-25 22.643501767396263 25.6504902023594 29.53605149974883 22.16995653049551 26-27 22.376412857458728 25.787919676529853 28.644649039028593 23.19101842698283 28-29 23.359298074771754 27.941255224241996 27.698347068067182 21.001099632919072 30-31 21.52936368149348 27.769246615105153 28.81591842675285 21.88547127664852 32-33 21.301141390715316 28.882481208170336 27.812581417025022 22.00379598408933 34-35 21.642168869053663 28.704181003455286 29.21769347806303 20.43595664942802 36-37 21.105356101487484 29.145668547585608 29.658543396660342 20.090431954266567 38-39 21.25477381387317 27.695874215754046 29.130351074091692 21.91900089628109 40-41 22.739695286772204 27.306073138140146 27.608315593824777 22.345915981262873 42-43 21.925909912135765 28.226948164273892 28.58919141445221 21.257950509138137 44-45 21.437310777489934 27.025105915323593 28.755365582406966 22.782217724779503 46-47 22.608751790784172 27.148411222691003 28.093968262729497 22.14886872379533 48-49 21.280078405874264 28.716562140285863 28.947042029642372 21.056317424197495 50-51 22.66933818091498 27.528002767668607 27.67300639316993 22.129652658246478 52-53 21.477243546405763 26.96116597528984 27.24486897301585 24.316721505288545 54-55 23.04138727328325 26.92723719686958 28.763186307879803 21.268189221967372 56-57 22.128327113186298 27.071023053568847 27.98979077597628 22.81085905726857 58-59 21.937035645318996 27.641992163014812 28.019924850511863 22.401047341154324 60-61 22.955575774137156 27.32776827555564 28.152749941625554 21.56390600868165 62-63 21.687015774014924 27.125772712181355 28.547650194351622 22.6395613194521 64-65 23.18752935621603 27.28709052647904 28.48966242261418 21.035717694690753 66-67 22.307733765723746 29.55136317672874 27.151701534935587 20.989201522611932 68-69 22.542425795935536 29.232211399426873 27.148204379945838 21.077158424691746 70-71 22.05507730616686 29.535140936915283 27.162084066399846 21.24769769051801 72-73 21.99095287320795 29.71946151043867 26.875822934757267 21.41376268159612 74-75 22.404971889413947 28.95891575237969 26.83367919685167 21.8024331613547 76-77 22.122617639327025 28.370531019978966 26.740569137749738 22.766282202944268 78-79 21.798120481468498 28.37697749158107 27.206098101522652 22.61880392542778 80-81 21.329292548260888 28.435831069071995 27.714049504765054 22.52082687790207 82-83 20.771651408927934 28.4941453818131 27.687271801316726 23.04693140794224 84-85 20.790349298726287 29.134579067376855 27.443261731472735 22.631809902424127 86-87 20.642702795967498 29.151876165671137 27.644485707947513 22.56093533041385 88-89 20.98088475237517 29.020815702410225 27.581738068516664 22.41656147669794 90-91 21.482892795024075 28.894218556993728 26.842205056433617 22.780683591548577 92-93 21.436952108225178 28.842500533726856 26.79928402535396 22.92126333269401 94-95 21.75741240246601 29.121862488517316 26.61630749087672 22.504417618139954 96-97 21.750309441125992 29.85776554255563 26.439517488764473 21.9524075275539 98-99 21.91494578202778 29.481733108359037 26.647227906102373 21.95609320351081 100-101 22.038701624407338 29.471063996420334 26.332528192923455 22.157706186248877 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 21.0 1 17.5 2 26.5 3 44.5 4 39.0 5 26.0 6 27.5 7 31.0 8 29.5 9 28.5 10 29.0 11 24.5 12 24.5 13 29.0 14 38.5 15 45.0 16 51.0 17 80.5 18 112.5 19 153.5 20 218.5 21 295.0 22 416.5 23 585.5 24 878.0 25 1276.0 26 1780.5 27 2434.5 28 3251.0 29 4307.5 30 5835.0 31 8132.5 32 12667.5 33 21192.5 34 34907.5 35 54477.0 36 83528.5 37 118069.5 38 140601.0 39 145448.5 40 148960.0 41 153402.0 42 161331.5 43 175845.0 44 175262.0 45 164239.5 46 160330.0 47 156770.0 48 147439.5 49 131851.0 50 117688.0 51 113971.5 52 110303.5 53 99347.5 54 85360.5 55 70819.0 56 57287.5 57 43854.5 58 31627.0 59 22935.0 60 17241.0 61 13822.0 62 11026.0 63 8375.5 64 6266.0 65 4558.0 66 3247.5 67 2402.5 68 1905.5 69 1422.0 70 953.5 71 706.0 72 531.0 73 364.5 74 273.5 75 221.0 76 162.0 77 120.0 78 93.0 79 61.5 80 48.5 81 36.5 82 25.5 83 19.5 84 15.5 85 14.5 86 10.5 87 6.0 88 5.0 89 5.0 90 2.5 91 2.0 92 1.5 93 0.5 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15592643662753394 2 0.0 3 0.0018069856752031955 4 0.010020556926126812 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.464071375277085E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 5.913771300665003E-4 38-39 0.0018069856752031955 40-41 0.005256685600591115 42-43 0.0022997999502586126 44-45 0.0012484628301403897 46-47 0.0011334728326274591 48-49 0.0030718756478454326 50-51 8.542114100960562E-4 52-53 0.013782372559049827 54-55 0.025182809455331807 56-57 0.026562689425486972 58-59 0.01999183242474808 60-61 0.029158177940778837 62-63 0.005305967028096656 64-65 0.010102692638636048 66-67 0.026743387993007295 68-69 0.025724905157892768 70-71 0.028731072235730806 72-73 0.029519575075819475 74-75 0.036698236349126716 76-77 0.017839876757006094 78-79 0.009987702641123117 80-81 0.014455885401625564 82-83 0.0067515555682592125 84-85 0.010398381203669298 86-87 0.031802947883576245 88-89 0.014439458259123716 90-91 0.020041113852253625 92-93 0.047852266107880986 94-95 0.05462024881864205 96-97 0.038143824889289274 98-99 0.03367564212878683 100-101 0.0329364207162037 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3043743.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.46332431819318 #Duplication Level Percentage of deduplicated Percentage of total 1 85.30992991556768 40.490928711449186 2 7.3397048997935475 6.967335881174655 3 2.085204842197092 2.9691226108520223 4 1.1143902690860974 2.11570667034688 5 0.7962842981802403 1.8897149947006795 6 0.5517620956468966 1.5713077975304746 7 0.38028151893260764 1.2634597545719433 8 0.28475815207894273 1.0812454819497785 9 0.2507876572793069 1.071289432120286 >10 1.509779899626426 14.490597934847537 >50 0.21240005918433838 6.922153646326944 >100 0.1566725985487546 13.931479451005327 >500 0.0065392000518258685 2.1111914227125865 >1k 0.0014350835210666677 1.0114008956678966 >5k 0.0 0.0 >10k+ 6.951030525271283E-5 2.113065314743914 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 64048 2.104251245916623 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 3803 0.12494484586905005 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3627 0.11916249170839982 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0011498999751293063 0.0 0.0 2 0.0011827542601330007 0.0 0.0 3 0.001215608545136695 0.0 0.0 4 0.0013141714001477787 0.0 0.0 5 0.0016755685351884178 0.0 0.0 6 0.0018726942452105845 0.0 0.0 7 0.002332654235262307 0.0 0.0 8 0.0025297799452844738 0.0 0.0 9 0.0027269056553066405 0.0 0.0 10-11 0.004944569893056017 0.0 0.0 12-13 0.0067515555682592125 0.0 0.0 14-15 0.008722812668480882 0.0 0.0 16-17 0.0106776426262007 0.0 0.0 18-19 0.013010296861463008 0.0 0.0 20-21 0.015753629659271497 0.0 0.0 22-23 0.018792651022113235 0.0 0.0 24-25 0.02243947665752332 0.0 0.0 26-27 0.026365563715464807 0.0 0.0 28-29 0.030965163615982033 0.0 0.0 30-31 0.06286667435456936 0.0 0.0 32-33 0.07053814990293202 0.0 0.0 34-35 0.07766752974873371 0.0 0.0 36-37 0.08596323671216657 0.0 0.0 38-39 0.09535956222322317 0.0 0.0 40-41 0.1064971648394756 0.0 0.0 42-43 0.11858754172083516 0.0 0.0 44-45 0.1333062614024903 0.0 0.0 46-47 0.14886276535173962 0.0 0.0 48-49 0.1659305664111589 0.0 0.0 50-51 0.18426325744322042 0.0 0.0 52-53 0.2051914369905738 0.0 0.0 54-55 0.2286001150557061 0.0 0.0 56-57 0.2534379545184991 0.0 0.0 58-59 0.28121625248912274 0.0 0.0 60-61 0.31188572754007154 0.0 0.0 62-63 0.34664556107398026 0.0 0.0 64-65 0.38380375741315875 0.0 0.0 66-67 0.4247237693852602 0.0 0.0 68-69 0.4679764355926239 0.0 0.0 70-71 0.5156644302754865 0.0 0.0 72-73 0.5712538805017375 0.0 0.0 74-75 0.6351226105489196 0.0 0.0 76-77 0.7076977261220806 0.0 0.0 78-79 0.7887163929411911 0.0 0.0 80-81 0.8780800481512401 0.0 0.0 82-83 0.9808646787852983 0.0 0.0 84-85 1.1043146546866802 0.0 0.0 86-87 1.2396743089019013 0.0 0.0 88-89 1.3856130428883122 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAGAGC 13020 0.0 52.19527 6 CGGAAGA 13065 0.0 52.055264 4 TCGGAAG 13135 0.0 51.924183 3 GATCGGA 13605 0.0 50.107174 1 ATCGGAA 13610 0.0 50.077087 2 GGAAGAG 15380 0.0 44.371384 5 GCCGTCT 7900 0.0 42.605446 50-51 ATGCCGT 7960 0.0 42.069885 48-49 TATGCCG 8030 0.0 41.703148 48-49 TGCCGTC 8030 0.0 41.560726 50-51 GATCTCG 7940 0.0 41.0627 40-41 GTATGCC 8305 0.0 40.859894 46-47 TCGATCT 7935 0.0 40.849503 38-39 TCGTATG 8380 0.0 40.7469 44-45 TCTCGTA 8110 0.0 40.434536 42-43 GAGCACA 16840 0.0 40.327053 9 CTCGATC 7945 0.0 40.319866 38-39 ACTCGAT 8335 0.0 39.914764 36-37 CGTATGC 8475 0.0 39.844143 46-47 CCGTCTT 8400 0.0 39.702778 52-53 >>END_MODULE