FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_30521-1.341000000356df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_30521-1.341000000356df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043743
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT640482.104251245916623TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC38030.12494484586905005No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA36270.11916249170839982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGAGC130200.052.195276
CGGAAGA130650.052.0552644
TCGGAAG131350.051.9241833
GATCGGA136050.050.1071741
ATCGGAA136100.050.0770872
GGAAGAG153800.044.3713845
GCCGTCT79000.042.60544650-51
ATGCCGT79600.042.06988548-49
TATGCCG80300.041.70314848-49
TGCCGTC80300.041.56072650-51
GATCTCG79400.041.062740-41
GTATGCC83050.040.85989446-47
TCGATCT79350.040.84950338-39
TCGTATG83800.040.746944-45
TCTCGTA81100.040.43453642-43
GAGCACA168400.040.3270539
CTCGATC79450.040.31986638-39
ACTCGAT83350.039.91476436-37
CGTATGC84750.039.84414346-47
CCGTCTT84000.039.70277852-53
Produced by FastQC (version 0.11.2)