##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_30484-1.34100000035806.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8315787 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.683059823441845 33.0 33.0 33.0 33.0 33.0 2 32.66236581095692 33.0 33.0 33.0 33.0 33.0 3 32.722993145447326 33.0 33.0 33.0 33.0 33.0 4 36.66536492577311 37.0 37.0 37.0 37.0 37.0 5 36.66815167343752 37.0 37.0 37.0 37.0 37.0 6 36.5488745683361 37.0 37.0 37.0 37.0 37.0 7 36.469464164967185 37.0 37.0 37.0 37.0 37.0 8 36.42053975167955 37.0 37.0 37.0 37.0 37.0 9 36.43972759283036 37.0 37.0 37.0 37.0 37.0 10-11 36.30369843527738 37.0 37.0 37.0 37.0 37.0 12-13 36.34107589576308 37.0 37.0 37.0 37.0 37.0 14-15 38.21947892604753 40.0 37.0 40.0 37.0 40.0 16-17 38.19510191879614 40.0 37.0 40.0 37.0 40.0 18-19 38.09257373956308 40.0 37.0 40.0 37.0 40.0 20-21 38.08249117010813 40.0 37.0 40.0 35.0 40.0 22-23 37.9434018091132 40.0 37.0 40.0 33.0 40.0 24-25 37.97901882287269 40.0 37.0 40.0 33.0 40.0 26-27 37.90592291505302 40.0 37.0 40.0 33.0 40.0 28-29 37.73991523592415 40.0 37.0 40.0 33.0 40.0 30-31 37.636655195713885 40.0 37.0 40.0 33.0 40.0 32-33 37.51931344561856 40.0 37.0 40.0 33.0 40.0 34-35 37.40856343482584 40.0 37.0 40.0 33.0 40.0 36-37 37.26439716409283 40.0 37.0 40.0 33.0 40.0 38-39 37.22348997154448 40.0 37.0 40.0 33.0 40.0 40-41 37.12697830043025 40.0 37.0 40.0 33.0 40.0 42-43 36.97397504289131 38.5 37.0 40.0 33.0 40.0 44-45 36.86091256305627 37.0 37.0 40.0 33.0 40.0 46-47 36.834365887437954 37.0 37.0 40.0 33.0 40.0 48-49 36.6088373235149 37.0 37.0 40.0 33.0 40.0 50-51 36.50165516504932 37.0 37.0 40.0 33.0 40.0 52-53 36.342765573480904 37.0 37.0 40.0 33.0 40.0 54-55 35.94132064710171 37.0 37.0 40.0 30.0 40.0 56-57 35.812502653086234 37.0 37.0 40.0 27.0 40.0 58-59 35.6773679989639 37.0 37.0 40.0 27.0 40.0 60-61 35.31635334094055 37.0 33.0 40.0 27.0 40.0 62-63 35.071384584525795 37.0 33.0 38.5 27.0 40.0 64-65 34.84064935766152 37.0 33.0 37.0 27.0 40.0 66-67 34.27660087974836 37.0 33.0 37.0 27.0 40.0 68-69 34.05045000551361 37.0 33.0 37.0 27.0 40.0 70-71 33.73806051068888 37.0 33.0 37.0 27.0 38.5 72-73 33.16957980044462 37.0 33.0 37.0 24.5 37.0 74-75 32.568161498123985 37.0 33.0 37.0 22.0 37.0 76-77 29.95391777110212 33.0 27.0 35.0 22.0 37.0 78-79 32.22374003807457 33.0 33.0 37.0 22.0 37.0 80-81 32.56029669831611 37.0 33.0 37.0 22.0 37.0 82-83 32.43800989611687 37.0 33.0 37.0 22.0 37.0 84-85 32.30611516384438 37.0 33.0 37.0 22.0 37.0 86-87 32.172895301430884 33.0 33.0 37.0 22.0 37.0 88-89 31.996142457713262 33.0 33.0 37.0 22.0 37.0 90-91 31.88987103686037 33.0 33.0 37.0 22.0 37.0 92-93 31.722651145345594 33.0 33.0 37.0 18.5 37.0 94-95 31.592643486419263 33.0 33.0 37.0 15.0 37.0 96-97 31.426594019303284 33.0 33.0 37.0 15.0 37.0 98-99 31.298518408420033 33.0 33.0 37.0 15.0 37.0 100-101 29.647309448883192 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11681715556213135 2306 2 0.1785838000245903 2306 3 0.13345845274674417 2306 4 0.1277985795404959 2306 5 0.11931534531370147 2306 6 0.14600162166912156 2306 7 0.2234835364502743 2306 8 0.12081067077821928 2306 9 0.19594709007819233 2306 10-11 0.24373614368418117 2306 12-13 0.22339782050370616 2306 14-15 0.4241639347196795 2306 16-17 0.4364476179015355 2306 18-19 0.497821078373164 2306 20-21 0.48803388708950735 2306 22-23 0.4579565926402367 2306 24-25 0.3792947026350575 2306 26-27 0.3870446921159214 2306 28-29 0.41383761257595353 2306 30-31 0.5051510822085987 2306 32-33 0.5371374778146887 2306 34-35 0.4499371945256101 2306 36-37 0.5062084540896095 2306 38-39 0.49636883621278827 2306 40-41 0.648230067067658 2306 42-43 0.5898178584942642 2306 44-45 0.5438105420381163 2306 46-47 0.5005681617505431 2306 48-49 0.4493985938802325 2306 50-51 0.5237529824311835 2306 52-53 0.4812151279703514 2306 54-55 0.6607657628869035 2306 56-57 0.6303420748771629 2306 58-59 0.4522446173967012 2306 60-61 0.4966544486468578 2306 62-63 0.3736197494522955 2306 64-65 0.3336785881342834 2306 66-67 0.5674296023555883 2306 68-69 0.4109411878125897 2306 70-71 0.34707455979697244 2306 72-73 0.29352193570557006 2306 74-75 0.4889726392020748 2306 76-77 0.14768123973593106 2306 78-79 0.49237770452237584 2306 80-81 0.6295788834459444 2306 82-83 0.5509282421921924 2306 84-85 0.6204938812184722 2306 86-87 0.7008319228636353 2306 88-89 0.63141724252975 2306 90-91 0.5491094877734177 2306 92-93 0.4839772212217106 2306 94-95 0.5396766014518342 2306 96-97 0.6693236537036711 2306 98-99 0.6566909177272642 2306 100-101 0.4379217643842104 2307 1 0.13140467732755212 2307 2 0.19905205335702902 2307 3 0.10731097471671092 2307 4 0.163918616100176 2307 5 0.13687156540461132 2307 6 0.1481284697626819 2307 7 0.18766980770072905 2307 8 0.21032655401365474 2307 9 0.14544231025274712 2307 10-11 0.18744155385363115 2307 12-13 0.17422958608754158 2307 14-15 0.2515339580590563 2307 16-17 0.33605982340554164 2307 18-19 0.46905999700734924 2307 20-21 0.36759066936503615 2307 22-23 0.4232315499576629 2307 24-25 0.3756353695219943 2307 26-27 0.3726429059034544 2307 28-29 0.3179697192017201 2307 30-31 0.42960213416781556 2307 32-33 0.5045996701542279 2307 34-35 0.4117820998646735 2307 36-37 0.47237702629418976 2307 38-39 0.43529583213802425 2307 40-41 0.5216397577646887 2307 42-43 0.4947871785538851 2307 44-45 0.37970862949693185 2307 46-47 0.3157686216961011 2307 48-49 0.48377305430968676 2307 50-51 0.3954287277623507 2307 52-53 0.431644918830294 2307 54-55 0.5222925187469585 2307 56-57 0.5496949906338031 2307 58-59 0.4017275399764131 2307 60-61 0.361037456602574 2307 62-63 0.40643069398294784 2307 64-65 0.468294621090223 2307 66-67 0.4782529497472652 2307 68-69 0.40910684568878963 2307 70-71 0.39267410352363896 2307 72-73 0.2947193461577555 2307 74-75 0.33371119311098596 2307 76-77 0.12862390667391566 2307 78-79 0.4393508942458553 2307 80-81 0.5791870616039461 2307 82-83 0.6355176903223452 2307 84-85 0.56321080047897 2307 86-87 0.5559715436765273 2307 88-89 0.6003624658787245 2307 90-91 0.5308706038454396 2307 92-93 0.46572287613097885 2307 94-95 0.5538426088092372 2307 96-97 0.7470719792845557 2307 98-99 0.5373572904302328 2307 100-101 0.39215777715721245 2201 1 0.13519850022088065 2201 2 0.18641239466188608 2201 3 0.14610849872390475 2201 4 0.12426942705391752 2201 5 0.12070614690499326 2201 6 0.11910102764490915 2201 7 0.16859348987679112 2201 8 0.15845476014952453 2201 9 0.15552675504833502 2201 10-11 0.20520716022166852 2201 12-13 0.1890154587306654 2201 14-15 0.2985259344073725 2201 16-17 0.24876386974977294 2201 18-19 0.2663321173330502 2201 20-21 0.32440028268388943 2201 22-23 0.3356140956211604 2201 24-25 0.24497825587776134 2201 26-27 0.2411485395082451 2201 28-29 0.3530646115387768 2201 30-31 0.34800557042922264 2201 32-33 0.3946902895350206 2201 34-35 0.38049903894410164 2201 36-37 0.3689383664827446 2201 38-39 0.37963033844564364 2201 40-41 0.5384890489057312 2201 42-43 0.2895850071061261 2201 44-45 0.29343555175226754 2201 46-47 0.3620151655159205 2201 48-49 0.3188605846202748 2201 50-51 0.26120068942982044 2201 52-53 0.34373188952294953 2201 54-55 0.497391503408096 2201 56-57 0.4455652930585714 2201 58-59 0.3795944223406096 2201 60-61 0.2596772429025265 2201 62-63 0.1504357222579742 2201 64-65 0.18878451089747728 2201 66-67 0.21552615413956744 2201 68-69 0.2653114409989925 2201 70-71 0.2396892653327498 2201 72-73 0.21648585018981947 2201 74-75 0.2759078538959585 2201 76-77 0.1276583380677181 2201 78-79 0.3793700962268005 2201 80-81 0.4163985037355076 2201 82-83 0.4836254238987081 2201 84-85 0.47814340498263164 2201 86-87 0.3646827544867577 2201 88-89 0.40149872027929945 2201 90-91 0.4508444462262524 2201 92-93 0.38773403353917146 2201 94-95 0.4734148898387893 2201 96-97 0.4149133704953414 2201 98-99 0.3668165624390376 2201 100-101 0.329180535910357 1101 1 0.09209459761952132 1101 2 0.13946563546490864 1101 3 0.08544988713358492 1101 4 0.1097579909416595 1101 5 0.12106315179698157 1101 6 0.08492352745011544 1101 7 0.1441494149189566 1101 8 0.12187329175876727 1101 9 0.15479528267476184 1101 10-11 0.14224788264247223 1101 12-13 0.14737697655480275 1101 14-15 0.24174033438240627 1101 16-17 0.2307166577669264 1101 18-19 0.30077388713245057 1101 20-21 0.30760866229228867 1101 22-23 0.23441587696954258 1101 24-25 0.2159820364438758 1101 26-27 0.2281914429412879 1101 28-29 0.2510266186439054 1101 30-31 0.2381369707400367 1101 32-33 0.2648331475266801 1101 34-35 0.294276560818588 1101 36-37 0.30537658125636113 1101 38-39 0.3281972395103381 1101 40-41 0.42519455723447663 1101 42-43 0.2607566990455652 1101 44-45 0.33989608772479585 1101 46-47 0.3138138049990502 1101 48-49 0.26369427144827284 1101 50-51 0.17588470809773327 1101 52-53 0.20779647414977376 1101 54-55 0.4385719706007265 1101 56-57 0.3754630796668863 1101 58-59 0.36672188528429217 1101 60-61 0.2313627648989467 1101 62-63 0.14764717856693466 1101 64-65 0.18824061040612605 1101 66-67 0.34735918936902266 1101 68-69 0.24274723917240948 1101 70-71 0.1385762462452007 1101 72-73 -0.06955249060428059 1101 74-75 0.016792816876964878 1101 76-77 -0.18397927749996157 1101 78-79 0.22972531621508807 1101 80-81 0.317952249118008 1101 82-83 0.3254778602342796 1101 84-85 0.23543525586592295 1101 86-87 0.28624990766005 1101 88-89 0.22488412996928275 1101 90-91 0.14933161837216957 1101 92-93 0.21267101726061455 1101 94-95 0.21056252050352242 1101 96-97 0.2369553442809753 1101 98-99 0.17160398813789257 1101 100-101 0.23811247900579247 2304 1 0.08977043842991606 2304 2 0.1715177879333325 2304 3 0.08651691717714982 2304 4 0.08684447468921519 2304 5 0.07732746723910822 2304 6 0.052139037955583944 2304 7 0.11529671972275679 2304 8 0.1338540900306171 2304 9 0.105816403613197 2304 10-11 0.1012899481706313 2304 12-13 0.10505034143610459 2304 14-15 0.17667734815582747 2304 16-17 0.17747079723504555 2304 18-19 0.33812573960180003 2304 20-21 0.2921955955056248 2304 22-23 0.28840776045976213 2304 24-25 0.1747985311676814 2304 26-27 0.11302954416407118 2304 28-29 0.2792620073965679 2304 30-31 0.26798240091917336 2304 32-33 0.2552331428515515 2304 34-35 0.30463139729382505 2304 36-37 0.2894463495176538 2304 38-39 0.28322906907840917 2304 40-41 0.3999662922136338 2304 42-43 0.28573288079680736 2304 44-45 0.2499984413098133 2304 46-47 0.19449673258710476 2304 48-49 0.17936578965257866 2304 50-51 0.1755495638957072 2304 52-53 0.19751210840872346 2304 54-55 0.4455665562138975 2304 56-57 0.4037816582618703 2304 58-59 0.31387687002307985 2304 60-61 0.28024341463223834 2304 62-63 0.12971540609733978 2304 64-65 0.15325743400481429 2304 66-67 0.2911766758395018 2304 68-69 0.15361129975111965 2304 70-71 0.26625398374961406 2304 72-73 0.10189799304840363 2304 74-75 0.2603521355315337 2304 76-77 0.0505401499064142 2304 78-79 0.3522647703728481 2304 80-81 0.48511705936781624 2304 82-83 0.41840482436536064 2304 84-85 0.39753682980399674 2304 86-87 0.5776125546989448 2304 88-89 0.46780375483121617 2304 90-91 0.3578984146591573 2304 92-93 0.2791529789870957 2304 94-95 0.353333843142952 2304 96-97 0.3560207201529657 2304 98-99 0.3952888624370061 2304 100-101 0.4691991867127818 2202 1 0.09180016864597462 2202 2 0.13631577816563833 2202 3 0.10104446011712298 2202 4 0.0972929705174721 2202 5 0.045292726242820436 2202 6 0.06495943999901499 2202 7 0.09599060816725569 2202 8 0.07577138999346289 2202 9 0.004130035058764747 2202 10-11 0.1376307364021656 2202 12-13 0.09621092717818414 2202 14-15 0.06905996446815976 2202 16-17 0.11818119157398854 2202 18-19 0.17370656853952937 2202 20-21 0.11363703098489708 2202 22-23 0.1433961409672193 2202 24-25 0.14508039453007626 2202 26-27 0.03748246166102831 2202 28-29 0.11124448624953942 2202 30-31 0.1513823160474388 2202 32-33 0.1974167906592612 2202 34-35 0.20524094212496635 2202 36-37 0.20168391125928053 2202 38-39 0.23346941952924993 2202 40-41 0.35374513810370445 2202 42-43 0.20592071647296706 2202 44-45 0.2552043618264008 2202 46-47 0.29903767080107 2202 48-49 0.19788987735502417 2202 50-51 0.23907254652970522 2202 52-53 0.166691751234481 2202 54-55 0.37902402828224524 2202 56-57 0.39561534964460776 2202 58-59 0.2976524541788521 2202 60-61 0.31469434982547284 2202 62-63 0.16596675624145263 2202 64-65 0.18729321295950996 2202 66-67 0.11311487872232107 2202 68-69 0.1006803420411444 2202 70-71 0.13727855617879925 2202 72-73 0.0762920613881235 2202 74-75 0.10676009735598768 2202 76-77 -0.06796598430840817 2202 78-79 0.23964382311064725 2202 80-81 0.28813784263661546 2202 82-83 0.2770566695274468 2202 84-85 0.32159434241734886 2202 86-87 0.39057351005774876 2202 88-89 0.30922230547427176 2202 90-91 0.22781094415153902 2202 92-93 0.17002590014487495 2202 94-95 0.22948072944597797 2202 96-97 0.3426237412830204 2202 98-99 0.300778338403628 2202 100-101 0.3129176610704647 1102 1 0.08968128178617008 1102 2 0.13032667306379153 1102 3 0.05879441916901129 1102 4 0.07921039979525091 1102 5 0.05850035717866575 1102 6 0.062091632861189794 1102 7 0.017346070161444516 1102 8 0.051465151179549196 1102 9 0.09690357197433741 1102 10-11 0.16620619758023025 1102 12-13 0.1087308111812888 1102 14-15 0.1212589649504352 1102 16-17 0.11643808002679634 1102 18-19 0.15727178593701296 1102 20-21 0.17253895592985202 1102 22-23 0.04705222761910477 1102 24-25 0.04998213719908762 1102 26-27 0.07576527254105514 1102 28-29 0.08939242358813004 1102 30-31 0.1718075042096885 1102 32-33 0.10295508623976701 1102 34-35 0.04311505747521949 1102 36-37 0.06940268363500479 1102 38-39 0.02635066383135154 1102 40-41 0.13810361312506814 1102 42-43 -0.021277425591939902 1102 44-45 -0.01782498559724388 1102 46-47 -0.03713185514780548 1102 48-49 0.05668408602714692 1102 50-51 0.0835355838954186 1102 52-53 0.01835459098292347 1102 54-55 0.09445882218814461 1102 56-57 0.1394984061137592 1102 58-59 0.03763462575653875 1102 60-61 0.032843866438753366 1102 62-63 -0.0629699920124196 1102 64-65 -0.08377765435270135 1102 66-67 0.005585209472137365 1102 68-69 -0.0187967019234776 1102 70-71 -0.13922692837950734 1102 72-73 -0.227838013831267 1102 74-75 -0.078483589028707 1102 76-77 -0.3993965309855554 1102 78-79 0.01924532408629176 1102 80-81 0.19364021087328354 1102 82-83 0.10425937737748825 1102 84-85 0.0620524557730846 1102 86-87 0.05540494674486496 1102 88-89 0.03972215533121215 1102 90-91 -0.08890473820602196 1102 92-93 -0.09749097708700916 1102 94-95 -0.07895190956574183 1102 96-97 -0.024606808436796967 1102 98-99 0.12321961128125025 1102 100-101 0.013260757873499784 2305 1 0.0879694297344571 2305 2 0.1461335441192233 2305 3 0.07252261970894125 2305 4 0.06148331481577429 2305 5 0.023868680288302357 2305 6 -0.017232745995485743 2305 7 0.03694592161102861 2305 8 0.035584317437773905 2305 9 -0.011180246891044021 2305 10-11 0.0755671635511419 2305 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1213 98-99 -1.8296186446179767 1213 100-101 -1.679900935987252 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 33.0 10 51.0 11 44.0 12 104.0 13 353.0 14 1024.0 15 2418.0 16 4506.0 17 7487.0 18 11063.0 19 15055.0 20 20303.0 21 26169.0 22 32855.0 23 41937.0 24 52518.0 25 66288.0 26 85141.0 27 115980.0 28 174007.0 29 239705.0 30 213838.0 31 205174.0 32 266456.0 33 377022.0 34 571252.0 35 961724.0 36 1760130.0 37 2480274.0 38 582317.0 39 554.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.54188357477327 28.28255271708607 16.917380941282715 17.258182766857946 2 30.681678114170076 28.130326089400796 25.034647953344646 16.153347843084482 3 33.200571261725514 21.366335771633842 26.052668541690416 19.380424424950228 4 30.196236286923888 21.05708396783884 24.577558240810387 24.169121504426887 5 34.56430521849585 19.85035210738322 25.0969030351547 20.48843963896622 6 32.3886001409127 20.209223733123515 26.489952183719957 20.91222394224383 7 30.46822868358701 24.221399610163175 22.324862337142594 22.98550936910722 8 28.754380072505466 26.985275115872977 21.821614719087922 22.438730092533635 9 29.70676136846699 22.68411877312394 25.52156518679471 22.087554671614367 10-11 24.04450113981996 29.802915827449645 28.356137548977628 17.79644548375277 12-13 21.473565881377194 29.259659969645686 28.747838298407597 20.518935850569527 14-15 21.618958013234348 29.315036568396952 28.857274723366533 20.20873069500217 16-17 23.876128621380033 26.880101666865684 28.852753203034183 20.3910165087201 18-19 21.42845890593398 26.72062187259005 31.12471495482027 20.7262042666557 20-21 23.53694350785545 27.122486158702163 30.695648738853205 18.64492159458918 22-23 21.369983118829346 31.452039220883105 28.182541997894607 18.995435662392943 24-25 21.34220128533836 26.701032626256545 30.5884818839155 21.368284204489605 26-27 21.314927859503857 26.93280263190964 28.62887781998264 23.123391688603856 28-29 23.042888183643953 29.5692277832513 28.49620246406023 18.891681569044515 30-31 20.650222282028146 27.94868964296464 30.63013157984927 20.770956495157943 32-33 20.348753521464655 30.222581458616006 28.566959447133506 20.861705572785834 34-35 22.601258305437597 30.281571666037145 28.66853131279096 18.4486387157343 36-37 22.454645402128317 29.932702780786634 29.04347156846564 18.569180248619407 38-39 20.61812415479494 27.480321915537786 32.98371584358402 18.91783808608325 40-41 20.620485072576166 27.49788252617183 28.21004282089485 23.671589580357157 42-43 20.67148404903999 29.45072028039893 30.405933151273494 19.47186251928759 44-45 20.51988824500574 27.208177889245768 30.665389081917542 21.60654478383095 46-47 22.69837666023385 27.367715221912874 28.391880847977124 21.542027269876147 48-49 20.48795816164902 29.917473074621775 30.28597641148124 19.308592352247967 50-51 22.84704425438137 27.88625373223891 29.674812244136678 19.591889769243046 52-53 20.54838699590164 27.818018490000902 27.565220622937048 24.068373891160412 54-55 22.863230488725794 27.774238721282217 29.70163989560211 19.660890894389876 56-57 20.728535901588753 27.455645551497916 29.969418647570105 21.846399899343222 58-59 20.76842277198808 27.692415110697567 29.98257518278798 21.556586934526372 60-61 22.539961708068194 27.86087180575033 29.866630210762 19.73253627541947 62-63 20.232994558285107 27.880678770336832 29.828804163024035 22.057522508354026 64-65 22.375103263555292 27.642046694790512 29.937236684698977 20.045613356955215 66-67 20.660646648817234 31.41277839515228 27.812417509234006 20.114157446796483 68-69 20.504910183847116 31.788860530859424 27.79148300037842 19.91474628491504 70-71 20.3300063170586 32.20165581154656 27.706271390292397 19.762066481102444 72-73 20.326384741365985 32.05537702411648 27.54022370460455 20.078014529912988 74-75 20.576908536841003 31.496407456879588 27.555482119208563 20.371201887070846 76-77 20.753461115250953 30.963666160443864 27.697798396873587 20.585074327431595 78-79 20.646449065363434 30.55204462967247 28.023678890822296 20.777827414141804 80-81 20.379277736601324 30.714244263401685 27.835137162693428 21.071340837303563 82-83 20.21142797767743 30.55778641740664 27.794373946804708 21.436411658111222 84-85 20.41712678544326 30.313124106872475 27.986229990996865 21.283519116687405 86-87 20.422472776772786 30.08340131972595 28.510744336117988 20.983381567383276 88-89 20.669414641045847 30.363685653283763 28.16565493870458 20.801244766965805 90-91 20.818515215078943 30.112284626224987 27.77976271485507 21.289437443840995 92-93 20.58610877789203 30.173875636879576 27.630056920252272 21.609958664976123 94-95 20.26889959763783 30.175021107139077 27.573810615570444 21.982268679652645 96-97 20.163980268905842 30.546215330750027 27.653258266938558 21.63654613340558 98-99 20.468656911226336 30.297796860804077 27.557876875985492 21.675669351984094 100-101 20.68405185228982 30.243977198792876 27.161546123026696 21.91042482589061 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 25.0 1 16.5 2 18.5 3 40.5 4 44.5 5 37.5 6 44.0 7 40.0 8 39.5 9 42.5 10 51.5 11 66.5 12 62.0 13 64.5 14 91.0 15 122.0 16 150.5 17 228.5 18 351.0 19 524.5 20 820.5 21 1299.0 22 2077.5 23 3351.5 24 5206.0 25 7495.0 26 10711.5 27 15439.0 28 21184.0 29 27619.5 30 34968.5 31 43881.5 32 60347.0 33 88891.5 34 130880.0 35 191965.5 36 266467.5 37 353981.0 38 444870.0 39 501634.0 40 547425.0 41 560012.5 42 530578.0 43 523725.5 44 513243.5 45 501454.0 46 513072.5 47 485214.0 48 402060.0 49 321410.5 50 260606.5 51 213048.0 52 171726.0 53 136254.5 54 110442.5 55 88421.0 56 65853.5 57 45956.0 58 32598.5 59 23642.0 60 16915.5 61 11832.0 62 7904.0 63 5152.5 64 3448.0 65 2367.5 66 1636.0 67 1204.5 68 893.5 69 639.5 70 469.5 71 338.5 72 247.5 73 180.5 74 138.0 75 116.5 76 90.5 77 71.0 78 56.5 79 38.5 80 31.5 81 29.0 82 24.0 83 15.0 84 16.0 85 15.5 86 10.5 87 8.5 88 4.0 89 2.0 90 2.0 91 2.5 92 1.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16003295899714604 2 0.0 3 0.0019120258852228901 4 0.010979117189990556 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.0063300082120912E-5 22-23 1.5632916042702873E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.756331421187194E-4 38-39 0.0015332283041881666 40-41 0.005459495294913157 42-43 0.002477215926766763 44-45 0.001154430723153443 46-47 0.0010943041229892012 48-49 0.0031085452284913026 50-51 8.177217622336887E-4 52-53 0.014899371520699123 54-55 0.026563931952562035 56-57 0.028710451578425472 58-59 0.021441145618568632 60-61 0.030634502783681208 62-63 0.00583829287594788 64-65 0.01079873738949783 66-67 0.02833766665740717 68-69 0.02704494475387597 70-71 0.0310553889848309 72-73 0.03116962952514296 74-75 0.038739568485821003 76-77 0.019186398112409565 78-79 0.0104680410885945 80-81 0.01562690338268645 82-83 0.007395571820201743 84-85 0.01088291462972777 86-87 0.03426013677358499 88-89 0.015620890722670025 90-91 0.021423107638519363 92-93 0.048949065193709264 94-95 0.05553292791169375 96-97 0.03996615112917154 98-99 0.035949694238200186 100-101 0.035853491677937395 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8315787.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.942936691791935 #Duplication Level Percentage of deduplicated Percentage of total 1 89.01408675650345 40.89568552531713 2 5.273750731190295 4.845831919427343 3 1.6260504362941606 2.2411659675696987 4 0.803398922169797 1.4764202327800344 5 0.6140573960670291 1.4105800036317064 6 0.35879767362054965 0.9890531282586682 7 0.266219042406988 0.8561619228017604 8 0.24214859786611162 0.8900014161415161 9 0.15882795533448255 0.6567320427137 >10 1.29505540021571 12.279528893577067 >50 0.197182608759099 6.335969714390911 >100 0.12671559647502662 10.85558511062798 >500 0.012758796517052008 4.129810945065949 >1k 0.010897644090632949 8.417183009442212 >5k 2.6253389862117484E-5 0.06673671264427623 >10k+ 2.6189099638876332E-5 3.653553455610137 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 303652 3.6515124786144715 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002609494447128095 0.0 0.0 2 0.002777848927587972 0.0 0.0 3 0.0030183553282449394 0.0 0.0 4 0.0032829123689676032 0.0 0.0 5 0.0036677226100187512 0.0 2.405064006569673E-5 6 0.00396835561083996 0.0 2.405064006569673E-5 7 0.004762026733007952 0.0 2.405064006569673E-5 8 0.005411394014781764 0.0 2.405064006569673E-5 9 0.005916457456161395 0.0 2.405064006569673E-5 10-11 0.00973449656659075 0.0 3.0063300082120912E-5 12-13 0.013011396275541931 0.0 3.6075960098545096E-5 14-15 0.01699177720641474 0.0 5.4113940147817644E-5 16-17 0.021260765818075907 0.0 6.0126600164241824E-5 18-19 0.026618045892709854 0.0 6.0126600164241824E-5 20-21 0.03327406053089142 0.0 7.816458021351437E-5 22-23 0.04146330347326116 0.0 8.417724022993855E-5 24-25 0.05105349619945773 0.0 8.417724022993855E-5 26-27 0.06164179048838071 0.0 8.417724022993855E-5 28-29 0.07405192076228023 0.0 8.417724022993855E-5 30-31 0.10991142510023405 0.0 9.620256026278691E-5 32-33 0.12543611326264129 0.0 9.620256026278691E-5 34-35 0.14042567468358677 0.0 9.620256026278691E-5 36-37 0.156148780626536 0.0 9.620256026278691E-5 38-39 0.17256935513139043 0.0 9.620256026278691E-5 40-41 0.1903127148398582 0.0 9.620256026278691E-5 42-43 0.20922854325152868 0.0 9.620256026278691E-5 44-45 0.22943108090671394 0.0 1.1424054031205946E-4 46-47 0.24953741600163643 0.0 1.2025320032848365E-4 48-49 0.2707440678795645 0.0 1.2025320032848365E-4 50-51 0.2932434416610238 0.0 1.2025320032848365E-4 52-53 0.315670663522286 0.0 1.2025320032848365E-4 54-55 0.3400700378689353 0.0 1.2025320032848365E-4 56-57 0.36466181733611025 0.0 1.2025320032848365E-4 58-59 0.3903118249661758 0.0 1.2025320032848365E-4 60-61 0.41867354226364867 0.0 1.2025320032848365E-4 62-63 0.4480694370839464 0.0 1.2025320032848365E-4 64-65 0.47832514228659295 0.0 1.2025320032848365E-4 66-67 0.5086710373894858 0.0 1.2025320032848365E-4 68-69 0.5421856043210342 0.0 1.2025320032848365E-4 70-71 0.5780932099391194 0.0 1.2025320032848365E-4 72-73 0.6169951202453838 0.0 1.2025320032848365E-4 74-75 0.6589634871600247 0.0 1.32278520361332E-4 76-77 0.7030543230604631 0.0 1.4430384039418038E-4 78-79 0.7522318693347966 0.0 1.5031650041060456E-4 80-81 0.8056002396405777 0.0 1.5632916042702873E-4 82-83 0.8629429782172151 6.012660016424182E-6 1.5632916042702873E-4 84-85 0.9261240096698004 1.2025320032848364E-5 1.5632916042702873E-4 86-87 0.994836688337496 1.2025320032848364E-5 1.5632916042702873E-4 88-89 1.070259495583521 1.2025320032848364E-5 1.683544804598771E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 46840 0.0 72.04014 3 CGGAAGA 46730 0.0 71.863304 4 GATCGGA 48375 0.0 69.886475 1 GAAGAGC 48080 0.0 69.73225 6 ATCGGAA 49845 0.0 67.582726 2 GGAAGAG 54330 0.0 61.893963 5 CGGTGCG 140 0.0 47.569416 1 AGAGCAC 74970 0.0 44.891937 8 GAGCACA 75155 0.0 44.8383 9 AAGAGCA 75310 0.0 44.6199 7 GCCGTCT 37900 0.0 43.670456 50-51 ATGCCGT 37870 0.0 43.53765 48-49 CGTCTTC 38130 0.0 43.395622 52-53 TGCCGTC 37925 0.0 43.30972 50-51 CCGTCTT 37995 0.0 43.218468 52-53 TATGCCG 38240 0.0 43.184715 48-49 TCGTATG 38960 0.0 43.01742 44-45 TCTCGTA 38365 0.0 42.959904 42-43 CGTATGC 38685 0.0 42.859947 46-47 CTGCTTG 39225 0.0 42.85754 58-59 >>END_MODULE