FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_30484-1.34100000035806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_30484-1.34100000035806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8315787
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT3036523.6515124786144715TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG468400.072.040143
CGGAAGA467300.071.8633044
GATCGGA483750.069.8864751
GAAGAGC480800.069.732256
ATCGGAA498450.067.5827262
GGAAGAG543300.061.8939635
CGGTGCG1400.047.5694161
AGAGCAC749700.044.8919378
GAGCACA751550.044.83839
AAGAGCA753100.044.61997
GCCGTCT379000.043.67045650-51
ATGCCGT378700.043.5376548-49
CGTCTTC381300.043.39562252-53
TGCCGTC379250.043.3097250-51
CCGTCTT379950.043.21846852-53
TATGCCG382400.043.18471548-49
TCGTATG389600.043.0174244-45
TCTCGTA383650.042.95990442-43
CGTATGC386850.042.85994746-47
CTGCTTG392250.042.8575458-59
Produced by FastQC (version 0.11.2)