##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_30423-1.3410000003574b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2567359 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68112718166801 33.0 33.0 33.0 33.0 33.0 2 32.64625593849555 33.0 33.0 33.0 33.0 33.0 3 32.71877871384563 33.0 33.0 33.0 33.0 33.0 4 36.660184259388735 37.0 37.0 37.0 37.0 37.0 5 36.671846048799566 37.0 37.0 37.0 37.0 37.0 6 36.56835643164824 37.0 37.0 37.0 37.0 37.0 7 36.453248649682415 37.0 37.0 37.0 37.0 37.0 8 36.399014317826214 37.0 37.0 37.0 37.0 37.0 9 36.428694623541155 37.0 37.0 37.0 37.0 37.0 10-11 36.27523692635116 37.0 37.0 37.0 35.0 37.0 12-13 36.31866618575743 37.0 37.0 37.0 37.0 37.0 14-15 38.17451279700268 40.0 37.0 40.0 37.0 40.0 16-17 38.16006760254409 40.0 37.0 40.0 37.0 40.0 18-19 38.090719879845395 40.0 37.0 40.0 37.0 40.0 20-21 38.12224429851844 40.0 37.0 40.0 37.0 40.0 22-23 37.91075887711847 40.0 37.0 40.0 33.0 40.0 24-25 38.02281235308346 40.0 37.0 40.0 35.0 40.0 26-27 37.95809799097049 40.0 37.0 40.0 33.0 40.0 28-29 37.739956897340804 40.0 37.0 40.0 33.0 40.0 30-31 37.6663886897002 40.0 37.0 40.0 33.0 40.0 32-33 37.58080151626633 40.0 37.0 40.0 33.0 40.0 34-35 37.57743813779062 40.0 37.0 40.0 33.0 40.0 36-37 37.486926253788425 40.0 37.0 40.0 33.0 40.0 38-39 37.14296773454745 38.5 37.0 40.0 33.0 40.0 40-41 36.92346317752991 37.0 37.0 40.0 33.0 40.0 42-43 36.799161706640945 37.0 37.0 40.0 33.0 40.0 44-45 36.61515900191598 37.0 37.0 40.0 33.0 40.0 46-47 36.72534791589334 37.0 37.0 40.0 33.0 40.0 48-49 36.357780310427955 37.0 37.0 40.0 33.0 40.0 50-51 36.32859545548558 37.0 37.0 40.0 33.0 40.0 52-53 36.289686794873646 37.0 37.0 40.0 33.0 40.0 54-55 35.68438948351205 37.0 35.0 40.0 30.0 40.0 56-57 35.559828017819086 37.0 33.0 40.0 27.0 40.0 58-59 35.50484622524547 37.0 33.0 40.0 27.0 40.0 60-61 35.146596560901685 37.0 33.0 38.5 27.0 40.0 62-63 34.94051240983438 37.0 33.0 37.0 27.0 40.0 64-65 34.69091311343681 37.0 33.0 37.0 27.0 40.0 66-67 33.77349291626142 37.0 33.0 37.0 27.0 40.0 68-69 33.719899320663764 37.0 33.0 37.0 27.0 37.0 70-71 33.437375333952126 37.0 33.0 37.0 27.0 37.0 72-73 32.58356700406916 37.0 33.0 37.0 22.0 37.0 74-75 31.583676260312643 35.0 33.0 37.0 18.5 37.0 76-77 29.00610218516382 33.0 27.0 33.0 15.0 37.0 78-79 31.112711545210466 33.0 33.0 37.0 15.0 37.0 80-81 31.495503161030463 33.0 33.0 37.0 15.0 37.0 82-83 31.258843426260214 33.0 33.0 37.0 15.0 37.0 84-85 31.16426646994051 33.0 33.0 37.0 15.0 37.0 86-87 31.057459046436435 33.0 33.0 37.0 15.0 37.0 88-89 30.942513882943523 33.0 33.0 37.0 15.0 37.0 90-91 30.877681305964614 33.0 33.0 37.0 15.0 37.0 92-93 30.666236587871037 33.0 33.0 37.0 15.0 37.0 94-95 30.49060513157685 33.0 33.0 37.0 15.0 37.0 96-97 30.326268745430617 33.0 27.0 37.0 15.0 37.0 98-99 30.196870207867306 33.0 27.0 37.0 15.0 37.0 100-101 28.62574926217954 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11156729375519348 2306 2 0.09846495403333932 2306 3 0.1694246568321276 2306 4 0.17142472664624364 2306 5 0.14910961210502904 2306 6 0.17528248997329854 2306 7 0.14848778586284084 2306 8 0.06785250974981238 2306 9 0.18661338085948387 2306 10-11 0.28399835885202407 2306 12-13 0.2201989588782638 2306 14-15 0.3863856347034371 2306 16-17 0.3477240930518448 2306 18-19 0.4534061660886124 2306 20-21 0.4678756239876307 2306 22-23 0.35150616308978044 2306 24-25 0.4001473572461265 2306 26-27 0.3677558386153805 2306 28-29 0.36820808037730757 2306 30-31 0.5618736557095261 2306 32-33 0.41214951606774264 2306 34-35 0.512043812123423 2306 36-37 0.44641787866518 2306 38-39 0.3644209472440352 2306 40-41 0.6139680590328993 2306 42-43 0.43633538964049023 2306 44-45 0.2826817810681135 2306 46-47 0.2753745931898095 2306 48-49 0.022151132551421426 2306 50-51 0.1929064168211383 2306 52-53 0.32435880650302096 2306 54-55 0.2992577524675042 2306 56-57 0.28841254065171285 2306 58-59 0.3242715096542881 2306 60-61 0.3671117539005593 2306 62-63 0.24163302430248734 2306 64-65 0.1542205515320063 2306 66-67 0.1451705796106424 2306 68-69 0.5044754828827962 2306 70-71 0.16880203055654164 2306 72-73 -0.02571686297488185 2306 74-75 0.18784533134385129 2306 76-77 -0.17536667082609014 2306 78-79 0.22085717219167478 2306 80-81 0.34617814978152595 2306 82-83 0.4249450796424945 2306 84-85 0.4569085631689198 2306 86-87 0.5512125449731968 2306 88-89 0.41583840896635493 2306 90-91 0.3013705655770842 2306 92-93 0.3246239270603333 2306 94-95 0.36151823816228656 2306 96-97 0.3881106760051338 2306 98-99 0.41898983177078364 2306 100-101 0.33184112450782166 2307 1 0.1146587144490141 2307 2 0.21273421167690998 2307 3 0.12223878760217843 2307 4 0.16116909114558098 2307 5 0.09242286473786976 2307 6 0.14302306494859351 2307 7 0.21237336993424805 2307 8 0.2208913681084823 2307 9 0.20365868518596386 2307 10-11 0.21972721274132567 2307 12-13 0.1985297427809698 2307 14-15 0.33239467416610324 2307 16-17 0.41008599889287467 2307 18-19 0.4914084196593933 2307 20-21 0.49399532762418374 2307 22-23 0.4739952886665435 2307 24-25 0.3719169768458954 2307 26-27 0.36817067189520714 2307 28-29 0.3897784666947359 2307 30-31 0.4862849260813604 2307 32-33 0.6050850752743386 2307 34-35 0.5831222527531637 2307 36-37 0.4655836168355094 2307 38-39 0.5097942027725253 2307 40-41 0.5321813802800435 2307 42-43 0.5992757959127175 2307 44-45 0.5060968850282279 2307 46-47 0.5406944680473842 2307 48-49 0.48378818175858385 2307 50-51 0.42047614846995174 2307 52-53 0.3771584590250612 2307 54-55 0.35235389432914843 2307 56-57 0.529998160603995 2307 58-59 0.43526301784709176 2307 60-61 0.24672165954015668 2307 62-63 0.35294966358637936 2307 64-65 0.2429955029039732 2307 66-67 0.22346516789333037 2307 68-69 0.3118486880521658 2307 70-71 0.3066909760994392 2307 72-73 0.018117606586180557 2307 74-75 0.1464432074478239 2307 76-77 -0.04993625769242982 2307 78-79 0.3124909480558067 2307 80-81 0.49447695564081684 2307 82-83 0.5620361478224538 2307 84-85 0.6754334814927141 2307 86-87 0.5614518137587261 2307 88-89 0.420586959567828 2307 90-91 0.4215132707433007 2307 92-93 0.47837568176104384 2307 94-95 0.5052472683085156 2307 96-97 0.5573078886276477 2307 98-99 0.49848768836059065 2307 100-101 0.13589220124882928 2201 1 0.1571197553176873 2201 2 0.20138831796963785 2201 3 0.10914264395988482 2201 4 0.16103319867318078 2201 5 0.19022541015612404 2201 6 0.18233066742758552 2201 7 0.20639692049442715 2201 8 0.20455523215736804 2201 9 0.15648003282202438 2201 10-11 0.2538310075245178 2201 12-13 0.23591215491182993 2201 14-15 0.39713052119065395 2201 16-17 0.37217199909248677 2201 18-19 0.4635007013616672 2201 20-21 0.5440547876292285 2201 22-23 0.3814757745638957 2201 24-25 0.42173975276259057 2201 26-27 0.36033148061894593 2201 28-29 0.37899616023295835 2201 30-31 0.45877797780338625 2201 32-33 0.5095752219423488 2201 34-35 0.5304353033361764 2201 36-37 0.5548122494358267 2201 38-39 0.44226734427380165 2201 40-41 0.5394792571434195 2201 42-43 0.48788499002525043 2201 44-45 0.569880920876777 2201 46-47 0.5562079188647431 2201 48-49 0.4540547960230086 2201 50-51 0.4344262719854939 2201 52-53 0.5215585896171646 2201 54-55 0.7421239541162024 2201 56-57 0.5844917468740078 2201 58-59 0.5372393229364079 2201 60-61 0.49505204289789617 2201 62-63 0.533652819731337 2201 64-65 0.3229264965937162 2201 66-67 0.305190675248717 2201 68-69 0.40507712668149054 2201 70-71 0.27115622230446235 2201 72-73 0.28117738066635667 2201 74-75 0.3488391157874986 2201 76-77 0.3091143501303506 2201 78-79 0.5114485231025689 2201 80-81 0.38491463653709346 2201 82-83 0.39793513291431637 2201 84-85 0.4141889432705632 2201 86-87 0.3297151136001091 2201 88-89 0.48244763764307663 2201 90-91 0.5142112494414484 2201 92-93 0.37911680321790087 2201 94-95 0.41063890082820365 2201 96-97 0.4278807402437792 2201 98-99 0.2703347997433596 2201 100-101 0.3841288413553805 1101 1 0.10493121731604305 1101 2 0.1543940866729301 1101 3 0.08978931176646654 1101 4 0.1302778358940202 1101 5 0.07710543629619337 1101 6 0.08782842842261118 1101 7 0.20152419976561475 1101 8 0.15451966197564815 1101 9 0.1434165199994979 1101 10-11 0.1971958570084169 1101 12-13 0.099972081357528 1101 14-15 0.28336560842254954 1101 16-17 0.28286325127434253 1101 18-19 0.31823939168850757 1101 20-21 0.3279634191544929 1101 22-23 0.2186989027663344 1101 24-25 0.2279128443074825 1101 26-27 0.27759417776024975 1101 28-29 0.2542456747958042 1101 30-31 0.23791813899870107 1101 32-33 0.27821150983437803 1101 34-35 0.2950082722271503 1101 36-37 0.37134269286293176 1101 38-39 0.3758340154620612 1101 40-41 0.5343465220560475 1101 42-43 0.2667004390916574 1101 44-45 0.41879463715748244 1101 46-47 0.4475185189963682 1101 48-49 0.24060298784265655 1101 50-51 0.2133272145341536 1101 52-53 0.20925579209101386 1101 54-55 0.4423790481821399 1101 56-57 0.4289770638781292 1101 58-59 0.44651102992182956 1101 60-61 0.4847918735908934 1101 62-63 0.26078059483754146 1101 64-65 0.10856008484895341 1101 66-67 0.4202911348475453 1101 68-69 0.3063961728623781 1101 70-71 0.12490201932798328 1101 72-73 -0.14436703883833957 1101 74-75 -0.25901492169316853 1101 76-77 -0.46498754015908617 1101 78-79 0.08849541240386927 1101 80-81 0.3644042200601483 1101 82-83 0.47158212719998005 1101 84-85 0.2773607704905423 1101 86-87 0.4355669181921833 1101 88-89 0.20582511678416182 1101 90-91 0.1612821938087059 1101 92-93 0.01889402541641516 1101 94-95 0.01341389149731853 1101 96-97 0.1308523251461473 1101 98-99 0.2534402546624719 1101 100-101 0.10085835275400967 2304 1 0.10802215730743825 2304 2 0.1859789947777628 2304 3 0.12590414050984577 2304 4 0.11948595910710225 2304 5 0.05175021484018316 2304 6 0.1057217464131881 2304 7 0.07127971886593087 2304 8 0.1556361866605016 2304 9 0.18889141588255853 2304 10-11 0.10375641359274113 2304 12-13 0.18525427335053735 2304 14-15 0.21395977118081788 2304 16-17 0.23443043744807568 2304 18-19 0.3869446222397386 2304 20-21 0.3652607457818249 2304 22-23 0.3422769831231278 2304 24-25 0.20762524896605328 2304 26-27 0.19510007052942058 2304 28-29 0.36209978414925104 2304 30-31 0.38064219151241474 2304 32-33 0.37457540652704324 2304 34-35 0.43536936075625476 2304 36-37 0.4172921894228736 2304 38-39 0.36226170297952365 2304 40-41 0.49583873064513995 2304 42-43 0.4196540424832591 2304 44-45 0.33743462356126486 2304 46-47 0.5156364446555628 2304 48-49 0.4609316013035283 2304 50-51 0.2601040054495485 2304 52-53 0.2991428416715465 2304 54-55 0.6472007329033929 2304 56-57 0.6081797538636167 2304 58-59 0.496363223662172 2304 60-61 0.5781143101914807 2304 62-63 0.3835916172380749 2304 64-65 0.4449637614684079 2304 66-67 0.43263397939676196 2304 68-69 0.2652683371203466 2304 70-71 0.24172932037635775 2304 72-73 -0.00960805494990069 2304 74-75 0.0911826513058216 2304 76-77 -0.14354233030335806 2304 78-79 0.17338033100226014 2304 80-81 0.31243968878180084 2304 82-83 0.2938472213779164 2304 84-85 0.32971719691357393 2304 86-87 0.30583528330220844 2304 88-89 0.19247582239859895 2304 90-91 0.21440818803796446 2304 92-93 0.2755517610010898 2304 94-95 0.22059387905324357 2304 96-97 0.38962586425211754 2304 98-99 0.3179417255540642 2304 100-101 0.3704918931459815 2202 1 0.14711476739365992 2202 2 0.18408447872512568 2202 3 0.08868782997576119 2202 4 0.08912946219219009 2202 5 0.10588965434448738 2202 6 0.0996738682451408 2202 7 0.08131180593039744 2202 8 0.09909216908938134 2202 9 0.12504518396568187 2202 10-11 0.2155315595199241 2202 12-13 0.1874077239460874 2202 14-15 0.157302551070849 2202 16-17 0.08612730447022443 2202 18-19 0.21483902899338148 2202 20-21 0.1989883362332634 2202 22-23 0.19469600427247968 2202 24-25 0.20184151005534545 2202 26-27 0.11330277854234083 2202 28-29 0.26043609718159644 2202 30-31 0.24833835069339472 2202 32-33 0.3402177853938184 2202 34-35 0.20932340833254415 2202 36-37 0.20346820766020102 2202 38-39 0.4133264574341098 2202 40-41 0.5284892054452186 2202 42-43 0.44321188117353927 2202 44-45 0.4317245313597482 2202 46-47 0.2744890133122553 2202 48-49 0.24938338092551504 2202 50-51 0.4197299946705826 2202 52-53 0.22684285234121404 2202 54-55 0.43079781514047966 2202 56-57 0.43853338818558285 2202 58-59 0.28204349285000063 2202 60-61 0.3003452069377559 2202 62-63 0.2701366497285065 2202 64-65 0.3281426449010212 2202 66-67 0.5099578193309711 2202 68-69 0.4317801635327072 2202 70-71 0.35785560756730206 2202 72-73 0.2810700412696505 2202 74-75 0.31068627769059987 2202 76-77 0.26340518275397073 2202 78-79 0.5252827842444105 2202 80-81 0.5691765410225607 2202 82-83 0.5421420268126802 2202 84-85 0.4355368483538484 2202 86-87 0.5204959229095643 2202 88-89 0.35119489013015226 2202 90-91 0.43987251362407775 2202 92-93 0.5118483984953315 2202 94-95 0.502183242698834 2202 96-97 0.5764269686925232 2202 98-99 0.5543932033026095 2202 100-101 0.5979249019653707 1102 1 0.11493571480782805 1102 2 0.14683922303918706 1102 3 0.10703869191984694 1102 4 0.11116741670472408 1102 5 0.022770430818482623 1102 6 0.026674302838799235 1102 7 0.06341176247102709 1102 8 0.09196946881414192 1102 9 0.16995841009924817 1102 10-11 0.20316794949529537 1102 12-13 0.08654983607124933 1102 14-15 0.17840902651629875 1102 16-17 0.14806327433840494 1102 18-19 0.304026049129547 1102 20-21 0.2547177292507854 1102 22-23 0.15619622156931 1102 24-25 0.18171460870810563 1102 26-27 0.19062133569140371 1102 28-29 0.36376363593286243 1102 30-31 0.41939412354577854 1102 32-33 0.3128044868279787 1102 34-35 0.19977480627547095 1102 36-37 0.27137694299266 1102 38-39 0.2853215320393545 1102 40-41 0.3313247841790954 1102 42-43 0.24840556507908218 1102 44-45 0.3296993249277662 1102 46-47 0.1404038329558901 1102 48-49 0.30574177582628437 1102 50-51 0.3585438437217192 1102 52-53 0.31758687667845464 1102 54-55 0.3645443021750978 1102 56-57 0.3222254061487888 1102 58-59 0.2484937318765148 1102 60-61 0.3759772509765824 1102 62-63 0.2564985213785107 1102 64-65 0.38336675036241274 1102 66-67 0.41377876674514624 1102 68-69 0.2842883483798744 1102 70-71 0.15694238143372985 1102 72-73 0.01450535924734453 1102 74-75 0.12157632549590147 1102 76-77 -0.041460238077554834 1102 78-79 0.3755238388583457 1102 80-81 0.48516060592188026 1102 82-83 0.49447724338518384 1102 84-85 0.3924173350987452 1102 86-87 0.4117388607626751 1102 88-89 0.3404699879137212 1102 90-91 0.22963957142503588 1102 92-93 0.1494894241363589 1102 94-95 0.127939290793865 1102 96-97 0.3213387461805688 1102 98-99 0.39701322358364877 1102 100-101 0.412227089420103 2305 1 0.08749365356406003 2305 2 0.12621563384902856 2305 3 0.0741330756601215 2305 4 0.027230908768217432 2305 5 0.04939195157031406 2305 6 0.05670748778416623 2305 7 0.09355394512312643 2305 8 0.08860528877007567 2305 9 0.08854881434404405 2305 10-11 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12 22.0 13 65.0 14 269.0 15 665.0 16 1483.0 17 2456.0 18 3491.0 19 4281.0 20 5953.0 21 8047.0 22 11036.0 23 14790.0 24 19932.0 25 26497.0 26 36097.0 27 54698.0 28 87417.0 29 102258.0 30 66375.0 31 56758.0 32 74847.0 33 108036.0 34 168793.0 35 304796.0 36 572729.0 37 720547.0 38 114967.0 39 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.574151949728524 21.607379516955934 21.392531141700445 12.4259373916151 2 31.51327882076484 27.297390041673175 29.58183876894505 11.607492368616933 3 38.11259182279967 14.858125765149424 32.163794852898505 14.865487559152404 4 31.503013568850857 14.218895107858964 29.859652457912876 24.418438865377304 5 38.89160027873001 13.037444315345068 32.599024912371036 15.47193049355388 6 33.885755751338245 13.73243866557034 36.74429637615931 15.637509206932105 7 33.68539421249619 23.614344546282776 25.404433115898478 17.29582812532256 8 30.04663547248359 33.42407509039445 19.900177575477368 16.629111861644592 9 38.36401531690738 23.749308141167635 20.37778900418679 17.508887537738197 10-11 30.23336432497364 28.889142500133406 25.053294066003236 15.824199108889719 12-13 25.359055745612512 27.236120854154017 24.801634675945202 22.603188724288266 14-15 24.19116298110237 28.042572152940043 24.624740832894815 23.14152403306277 16-17 27.88583521042441 24.901718069035145 25.356893991062414 21.85555272947804 18-19 22.206672304107062 25.056137454870942 29.906510932051184 22.830679308970815 20-21 26.11091787319187 25.677067367672386 30.26345361127914 17.94856114785661 22-23 22.011200645645786 33.65861011657147 25.743742969409727 18.586446268373017 24-25 22.712698146227307 23.016356497085138 31.10126008867478 23.16968526801277 26-27 20.583837320764257 23.103294085478502 27.49416813153127 28.818700462225966 28-29 24.622501177279844 29.41643533296278 25.34608521831189 20.61497827144548 30-31 18.52002388446649 26.011126609095182 30.4714299792121 24.997419527226228 32-33 19.08476765423145 28.82499097321411 26.614859861826883 25.475381510727562 34-35 20.029142788367345 24.080212389463256 30.726166461332443 25.164478360836956 36-37 23.58304465956945 26.54641790379125 26.39421783870454 23.476319597934754 38-39 23.70551335711836 30.83371781615833 24.2382711964294 21.22249763029391 40-41 24.671935998304757 29.989673501917668 24.015340556298696 21.323049943478882 42-43 21.144258404376462 30.333814582251208 29.016135179724365 19.50579183364797 44-45 19.047454896580952 26.956610818588395 28.8498871586813 25.14604712614935 46-47 24.217045730523935 26.362247043259067 25.357538421029158 24.06316880518784 48-49 19.958710843467355 29.745954831220832 30.42856975919852 19.866764566113286 50-51 23.96348823703316 25.405371402351935 28.545843416738826 22.085296943876088 52-53 19.033920196778162 26.274337477743543 24.708374895279476 29.983367430198815 54-55 24.279622947025725 26.27156416827902 29.876663749755515 19.57214913493974 56-57 20.193244846222914 25.59325345687476 28.527576586585656 25.68592511031667 58-59 19.001517268005944 24.847742379619326 29.493964140737177 26.656776211637556 60-61 22.82574920607894 24.815534260511306 31.228230607549058 21.130485925860697 62-63 18.85739293426945 24.832989769430448 30.98765273402077 25.32196456227933 64-65 24.586553228952717 26.49265352511007 29.332433466852482 19.588359779084726 66-67 20.240358062992204 34.87467308244897 24.55363395948901 20.331334895069816 68-69 19.67497790386638 32.97594448430439 26.62063475953153 20.728442852297697 70-71 19.27250915047297 35.84524888429298 25.34038123009475 19.541860735139295 72-73 21.14188818248516 35.23205148136329 24.597227141332567 19.02883319481898 74-75 22.549345854480627 33.02570796761907 24.799723339795232 19.62522283810507 76-77 21.673803858135074 31.324654165673227 25.165515054331483 21.836026921860217 78-79 21.681398175344558 32.18592475438829 25.22426433351772 20.908412736749433 80-81 21.165342195776525 32.39210779228318 25.165635151933998 21.276914860006304 82-83 20.563483536919325 31.389820103165473 26.057636948227948 21.989059411687258 84-85 21.148407034295964 30.698468651680734 25.921626426271544 22.231497887751765 86-87 19.312298420287185 31.32082382359273 27.204667151773027 22.16221060434706 88-89 19.075672793903525 31.521705042976496 27.23040570113714 22.172216461982842 90-91 20.489298542354582 30.526849532867395 25.977329931141256 23.006521993636767 92-93 20.633959987700994 30.72105456573324 25.074111644267226 23.570873802298543 94-95 20.987475093900258 31.73615367002645 24.954776855961143 22.321594380112145 96-97 20.379495032741627 32.973224149117115 23.871539041540267 22.775741776600988 98-99 20.606858204927885 30.728525978845976 25.468815448787513 23.195800367438625 100-101 21.785842452070376 31.377612151406296 24.93382803041904 21.902717366104287 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 232.0 1 157.0 2 95.5 3 205.0 4 240.0 5 161.0 6 154.5 7 166.5 8 177.0 9 191.0 10 194.5 11 199.0 12 200.5 13 193.0 14 196.0 15 202.5 16 215.5 17 226.5 18 245.5 19 277.0 20 293.0 21 337.0 22 370.0 23 408.5 24 521.0 25 647.0 26 769.5 27 968.0 28 1277.5 29 1647.0 30 2228.5 31 3281.5 32 5532.5 33 9579.5 34 16006.5 35 27240.5 36 44159.0 37 66822.0 38 93237.5 39 113609.5 40 128554.5 41 137688.5 42 169014.0 43 208198.5 44 201592.5 45 177765.0 46 171468.0 47 159917.5 48 132371.5 49 109562.5 50 92679.5 51 84218.5 52 82137.5 53 81760.5 54 68192.5 55 42196.5 56 27201.0 57 21895.5 58 18857.5 59 15055.0 60 11633.5 61 9031.0 62 6517.5 63 4581.5 64 3371.0 65 2378.0 66 1731.5 67 1385.0 68 1066.5 69 794.0 70 596.0 71 462.5 72 287.5 73 156.0 74 90.0 75 56.0 76 45.0 77 26.0 78 20.5 79 18.0 80 12.0 81 8.5 82 7.0 83 4.5 84 1.5 85 0.0 86 0.5 87 0.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16187841279696374 2 0.0 3 0.0019475266217151557 4 0.011802011327593844 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.7265372704012177E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 5.647827202973951E-4 38-39 0.002239655614972429 40-41 0.006894244240871651 42-43 0.0031355178609614003 44-45 0.001441169700069215 46-47 0.0018501502906293978 48-49 0.0038561027109960083 50-51 8.569117135546685E-4 52-53 0.01587234196697852 54-55 0.027927531755395328 56-57 0.030011385240630545 58-59 0.02237708088350714 60-61 0.03215366452451721 62-63 0.00588153039757977 64-65 0.012133090853285419 66-67 0.030050335773064853 68-69 0.02857021554056133 70-71 0.03213418925830007 72-73 0.032640546179946 74-75 0.04027485053706942 76-77 0.020371128463140528 78-79 0.01164620919785663 80-81 0.01643712468727591 82-83 0.008608067667980987 84-85 0.011977288723548207 86-87 0.035503410313867285 88-89 0.016846105277836095 90-91 0.022688685142981564 92-93 0.05055779109972544 94-95 0.05731570847707703 96-97 0.04105386118575548 98-99 0.036769302617982134 100-101 0.036613500488244924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2567359.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.834243313798403 #Duplication Level Percentage of deduplicated Percentage of total 1 82.92034382439198 26.397064009695153 2 7.446805402421848 4.741268301824111 3 2.439381743662834 2.329676158890014 4 1.6232973030564042 2.0670576526452127 5 0.9295845339856614 1.4796310117821723 6 0.6889761843220942 1.3159821293473177 7 0.4792856816621438 1.0680387904796689 8 0.3215986463560468 0.8190279645989281 9 0.29298658318086285 0.8394305558992222 >10 2.2530981528827274 14.296542585845575 >50 0.31399803727643844 7.019948263659714 >100 0.25796318794041756 16.407607266660758 >500 0.022653956994362526 4.729384759662658 >1k 0.009039987069256385 4.642940703186799 >5k 3.7004054885105627E-4 0.7515615033646182 >10k+ 6.167342479989576E-4 11.094838342458004 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 207576 8.085195720582902 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 27700 1.078929748430196 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 25366 0.9880192057285327 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11777 0.45872042047878775 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10133 0.3946857451567934 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 8728 0.33996024708659756 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5300 0.20643782190180648 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5112 0.1991151218041575 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 4946 0.19264933342006318 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 3654 0.14232524551494355 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3186 0.12409639633568972 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2948 0.11482616961632558 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004596162827247767 0.0 0.0 2 0.004946717619156495 0.0 0.0 3 0.005492025073236739 0.0 0.0 4 0.0058036293327111636 0.0 0.0 5 0.006699491578700135 0.0 0.0 6 0.007205848500346075 0.0 0.0 7 0.008179611811203653 0.0 0.0 8 0.009153375122061231 0.0 0.0 9 0.010399792159958931 0.0 0.0 10-11 0.015969718298064275 0.0 0.0 12-13 0.02116961437804374 0.0 0.0 14-15 0.026720065249931934 0.0 0.0 16-17 0.03166678286908843 0.0 0.0 18-19 0.03826889811670281 0.0 0.0 20-21 0.04370249739128809 0.0 0.0 22-23 0.04935032459426204 0.0 0.0 24-25 0.05542660765401333 0.0 0.0 26-27 0.06132761331781025 0.0 0.0 28-29 0.06857241235059063 0.0 0.0 30-31 0.12283050403157486 0.0 0.0 32-33 0.13194492862120177 0.0 0.0 34-35 0.1401440156986226 0.0 0.0 36-37 0.14939476715176958 0.0 0.0 38-39 0.15930767765629972 0.0 0.0 40-41 0.17126549111363076 0.0 0.0 42-43 0.18246376918849294 0.0 0.0 44-45 0.19574590074859027 0.0 0.0 46-47 0.20922278497085917 0.0 0.0 48-49 0.224238215224283 0.0 0.0 50-51 0.2410453699696848 0.0 0.0 52-53 0.2591768428178529 0.0 0.0 54-55 0.278457356372833 0.0 0.0 56-57 0.30143817050907173 0.0 0.0 58-59 0.3260743822737685 0.0 0.0 60-61 0.350944297233071 0.0 0.0 62-63 0.37939766117632945 0.0 0.0 64-65 0.4105775623899891 0.0 0.0 66-67 0.44621729956737644 0.0 0.0 68-69 0.4862000211111886 0.0 0.0 70-71 0.528792428328099 0.0 0.0 72-73 0.5736439664261991 0.0 0.0 74-75 0.6245717875840504 0.0 0.0 76-77 0.6845556075328771 0.0 0.0 78-79 0.7518037017807014 0.0 0.0 80-81 0.8276014378978553 0.0 0.0 82-83 0.9115008847613442 0.0 0.0 84-85 1.0062675301740038 0.0 0.0 86-87 1.1132062169723829 0.0 0.0 88-89 1.2283245155819658 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 28660 0.0 80.73791 3 GATCGGA 28855 0.0 80.36927 1 CGGAAGA 28550 0.0 80.31388 4 GAAGAGC 28585 0.0 80.19269 6 ATCGGAA 29055 0.0 79.46047 2 GGAAGAG 30260 0.0 75.957756 5 GAGCACA 36725 0.0 62.392326 9 AGAGCAC 36890 0.0 62.16475 8 AAGAGCA 36940 0.0 61.977768 7 GCGCACA 255 0.0 48.420452 9 GTACGGG 205 0.0 46.400597 1 ATCGGAC 125 0.0 45.595047 2 GCCGTCT 24765 0.0 45.51876 50-51 ATGCCGT 24700 0.0 45.425663 48-49 TCGTATG 25225 0.0 45.356037 44-45 TCTCGTA 24655 0.0 45.33465 42-43 AATCTCG 24695 0.0 45.131428 40-41 CTGCTTG 25220 0.0 45.104275 58-59 GTATGCC 25275 0.0 45.065327 46-47 TATGCCG 24935 0.0 44.854668 48-49 >>END_MODULE